Vegetation C–N–P accumulation and allocation patterns at the community level in early restored plantations in the loess hilly-gully region

Accumulation of vegetation biomass is a crucial process for carbon fixation in the early stage of afforestation and a primary driving force for subsequent ecological functions.Accurately assessing the storage and allocation of elements in plantations is essential for their management and estimating carbon sink capacity.However,current knowledge of the storage and allocation patterns of elements within plant organs at the community level is limited.To clarify the distribution patterns of elements in plant organs at the community level,we measured the biomass within plant organs of five typical plantations in the early stage of afforestation in the loess hilly-gully region.We assessed the main drivers of element accumulation and distribution by employing redundancy analysis and random forest.Results revealed significant differences in biomass storages among plantations and a significant effect of plantation type on the storages of elements within plant organs.Furthermore,the dominant factors influencing C–N–P storage and allocation at the community level were found to be inconsistent.While the storage of elements was mainly influenced by stand openness,total soil nitrogen,and plant diversity,the allocation of elements in organs was mainly influenced by stand openness and soil water content.Overall,the spatial structure of the community had an important influence on both element storage and allocation,but soil conditions played a more important role in element allocation than in storage.Random forest results showed that at the community level,factors influencing element storage and allocation within plant organs often differed.The regulation of elemental storage could be regulated by the major growth demand resources,while the allocation was regulated by other limiting class factors,which often differed from those that had a significant effect on element storage.The differences in plant organ elemental storage and allocation drivers at the community level reflect community adaptation strategies and the regulation of resources by ecosystems in combination with plants.Our study provides valuable insights for enhancing plantation C sink estimates and serves as a reference for regulating element storage and allocation at the local scale.

Planting density affected biomass and grain yield of maize for seed production in an arid region of Northwest China

Field experiments were conducted from 2012 to 2015 in an arid region of Northwest China to investigate the effects of planting density on plant growth, yield, and water use efficiency（WUE） of maize for seed production. Five planting densities of 6.75, 8.25, 9.75, 11.25 and 12.75 plants/m^2 were conducted in 2012, and a planting density of 14.25 plants/m^2 was added from 2013 to 2015. Through comparison with the Aqua Crop yield model, a modified model was developed to estimate the biomass accumulation and yield under different planting densities using adjustment coefficient for normalized biomass water productivity and harvest index. It was found that the modified yield model had a better performance and could generate results with higher determination coefficient and lower error. The results indicated that higher planting density increased the leaf area index and biomass accumulation, but decreased the biomass accumulation per plant. The total yield increased rapidly as planting density increased to 11.25 plants/m^2, but only a slight increase was observed when the density was greater than 11.25 plants/m^2. The WUE also reached the maximum when planting density was 11.25 plants/m^2, which was the recommended planting density of maize for seed production in Northwest China.

One-time fertilization at first flowering improves lint yield and dry matter partitioning in late planted short-season cotton

Cotton producers have substantially reduced their inputs(labor,nutrients,and management)mainly by adopting a shortseason cropping management that is characterized by late sowing,high density,and reduced fertilization with one-time application at the first bloom stage without lint yield reduction.However,it has been hypothesized that one-time fertilization at an earlier growth stage could be a more effective and economic management practice.A two-year field experiment was conducted by applying five fertilizer one-time fertilization at 0(FT1),5(FT2),10(FT3),15(FT4),and 20(FT5)days after the first flower appeared in the field and one three-split fertilizer application taken as the conventional control(FT6),making six treatments altogether.Cotton growth period,biomass accumulation,yield,and its formation were quantified.The results showed that the one-time fertilization did not affect the cotton growth progress as compared to FT6,however,the total crop cycles for FT3–FT5 were 3 days shorter.FT1 produced the highest cotton lint yield(1396 kg ha–1),which was similar to the FT6 but higher than the other treatments,and could be attributed to more bolls per unit area and higher lint percentage.Cotton yield was positively correlated with cotton plant biomass accumulated.FT1 had both the highest average(VT)(193.7 kg ha–1 d–1)and the highest maximum(VM)(220.9 kg ha–1 d–1)rates during the fast biomass accumulation period.These results suggest that one-time fertilizer application at the first flower stage might be an adjustment that is more effective than at first bloom,and allowed for easier decision making for application date due to non counting of plants with flowers is needed.

The Relative Contribution of Non-Foliar Organs of Cotton to Yield and Related Physiological Characteristics Under Water Defi cit

Water deficit is one of the most important causes of decreased yield in cultivated plants. Non-foliar green organs in cotton play an important role in yield formation at the late growth stage. Although better photosynthetic performance was observed in a non-foliar organ （bract） compared with leaves under water deficit. However, the physiological response of each organ in cotton to water deficit has not been comprehensively studied in relation to the water status and photosynthesis characteristics. We studied the maintenance of water status of each organ in cotton by measuring their relative water content, proline content and stomatal characteristics. Water deficit significantly decreased the surface area of each organ, but to a lesser extent in non-foliar organs. Our results showed that the relative contribution of biomass accumulation of non-foliar organs increased under water deficit. Non-foliar organs （bracts and capsule wall） showed less ontogenetic decrease in O2 evolution capacity and in RuBPC activity （per dry weight） as well as better antioxidant systems than leaves at various days after anthesis. We conclude that the photosynthesis from non-foliar organs is important for increasing cotton yield especially under water deficit conditions.

Effects of drip irrigation modes on growth and physiological characteristics of Arabica coffee under different N levels

The objective of this study was to obtain the water-saving and efficient production mode of Arabica coffee. The effects of three drip irrigation modes,conventional drip irrigation( CDI),alternate drip irrigation( ADI) and fixed drip irrigation( FDI) on growth,photosynthetic characteristics,biomass accumulation and irrigation water use efficiency of Arabica coffee were investigated under three nitrogen levels,high nitrogen( NH),middle nitrogen( NM) and low nitrogen( NL). The results show that there was a significant Logistic curve between the plant height,the stem diameter of Arabica coffee and growth days. Compared with CDI,ADI had no significant effects on leaf net photosynthetic rate,stomatal conductance,instantaneous water use efficiency and biomass accumulation above ground of Arabica coffee,while FDI decreased significantly,ADI and FDI increased irrigation water use efficiency by 50. 59% and 32. 85%,respectively. Compared with NH,with the reduction of N application rate,net photosynthetic rate,stomatal conductance,biomass accumulation above ground and irrigation water use efficiency decreased by 6. 81%-12. 30%,13. 70%-22. 69%,9. 61%-16. 67% and 9. 78%-15. 64%,respectively. Compared with CDINH,ADINHdecreased net photosynthesis rate and the stomatal conductance not significantly,other treatments decreased by 9. 16%-19. 22%,14. 49%-32. 91%,and decreased biomass accumulation above ground by 8. 26%-27. 34% except ADINH,and increased irrigation water use efficiency by 16. 46%-60. 95% except CDINMand CDINL. Therefore,alternate drip irrigation under high N level( ADINH) is the best water and nitrogen coupling mode of young Arabica coffee tree for water efficiency.

Effect of Interplanting with Zero Tillage and Straw Manure on Rice Growth and Rice Quality

The interplanting with zero-tillage of rice, i.e. direct sowing rice 10-20 days before wheat harvesting, and remaining about 30-cm high stubble after cutting wheat or rice with no tillage, is a new cultivation technology in wheat-rice rotation system. To study the effects of interplanting with zero tillage and straw manure on rice growth and quality, an experiment was conducted in a wheat-rotation rotation system. Four treatments, i.e. ZIS （Zero-tillage, straw manure and rice interplanting）, Zt （Zero-tillage, no straw manure and rice interplanting）, PTS （Plowing tillage, straw manure and rice transplanting）, and PT （Plowing tillage, no straw manure and rice transplanting）, were used. ZtS reduced plant height, leaf area per plant and the biomass of rice plants, but the biomass accumulation of rice at the late stage was quicker than that under conventional transplanting cultivation. In the first year （2002）, there was no significant difference in rice yield among the four treatments. However, rice yield decreased in interplanting with zero-tillage in the second year （2003）. Compared with the transplanting treatments, the number of filled grains per panicle decreased but l000-grain weight increased in interplanting with zero-tillage, which were the main factors resulting in higher yield, tnterplanting with zero-tillage improved the milling and appearance qualities of rice. The rates of milled and head rice increased while chalky rice rate and chalkiness decreased in interplanting with zero-tillage. Zero-tillage and interplanting also affected rice nutritional and cooking qualities. In 2002, ZIS showed raised protein content, decreased amylose content, softer gel consistency, resulting in improved rice quality. In 2003, zero-tillage and interplanting decreased protein content and showed similar amylose content as compared with transplanting treatments. Moreover, protein content in PTS was obviously increased in comparison with the other three treatments. The rice in interplanting with zero-tillage treatments had higher peak viscosity and breakdown, lower setback, showing better rice taste quality. The straw manure had no significant effect on rice viscosity under interplanting with zero-tillage, but had the negative influence on the rice taste quality under transplanting with plowing tillage.

The efficacy of chemical topping in field-grown cotton is mediated by drip irrigation amount in irrigated agricultural area

Background:Cotton production in China is challenged by high labor input including manual topping(MT).Recently,to replace MT in the Xinjiang cotton region of China,mepiquat chloride(MC)was applied once more than the traditional multiple-application;this was designated as chemical topping(CT),but it is unclear whether the amount of irrigation needs to be adjusted to accommodate CT.Results:The main plots were assigned to three drip irrigation amounts[300(I_(1))480(I_(2)), and 660(I_(3))mm],and the subplots were assigned to the CT treatments[450(MC)750(MC_(2)),and 1050(MC_(3))mL·hm^(-2)25%MC]with MT as a control that was performed after early bloom.The optimum drip irrigation amount for CT was explored based on leaf photosynthesis,chlorophyll fluorescence,biomass accumulation,and yield.There were significant influe nces of drip irrigation,topping treatme nts and their interaction on chlorophyll fluorescence characteristics,gas exchange parameters and biomass accumulation characteristics as well as yield.The combination of I_(2) and MC_(2)(I_(2)MC_(2))performed best.Compared with I_(2)MC_(2)the net photosynthetic rate(Pn),stomatai conductance(Gs),transpiration rate(Tr),and photochemical quenching coefficient(qP)of I_(2)MC_(2)significantly increased by 4.0%~7.2%,6.8%〜17.1%,5.2%~17.6%,and 4.8%~9.6%,respectively,from the peak flowering to boll opening stages.Moreover,I_(2)MC_(2) showed fast reproductive organ biomass accumulation and the highest seed cotton yield;the latter was 6.6%~12.8%higher than that of I_(2)MT.Further analysis revealed that a 25%MC emulsion in water(MCEW)application resulted in yield improvement by increasing Pn,φPSⅡ,and qP to promote biomass accumulation and transport to reproductive organs.Conclusion:The results showed that the 480 mm drip irrigation combined with 750 mL·hm^(-2)MC increased the rate of dry matter accumulation in reproductive organs by increasing Pn,φPSⅡ and qP to improve photosynthetic performance,thus achieving higher yield.

Contribution of Biofertiliser (<i>Frateuria auranta</i>) in an Integrated Potassium Management Package on Growth of Apple Banana

Bananas demand high amounts of potassium for optimal growth and productivity, yet deficiencies are widespread amid the low input production strategy of smallholder farmers in Uganda. Of the potassium pool in the soil, 90% - 98% is unavailable for plant uptake. Judicious application of fertilisers is required to alleviate soil fertility problems complemented with manures and biofertilisers in an integrated nutrient management (INM) package. Biofertilisers such as potassium solubilising bacteria (KSB) have potential to solubilise unavailable forms of K in soil to forms that are readily absorbed by the plants. However, the added value of each component in this integrated K management package in apple bananas is not known. Therefore, an experiment was set up to quantify the relative contribution of mineral K, manure and KSB on the growth of apple bananas. Potted tissue culture plantlets of apple banana (cv. Sukali ndiizi) were used. The treatments comprised of a full factorial combination of mineral fertiliser (Muriate of potash, 60% K2O), animal manure and KSB (Frateuria auranta). The manure and muriate of potash were applied to supply a total of 150 kg⋅K⋅ha−. Soil microbiological assays were run to evaluate the contribution of indigenous microbial K solubilising activity in the soil to the experimental INM package. Data on pseudostem height, girth at collar and 30-cm height, leaf length and width at the widest point were collected once a week for 24 weeks. Bacillus, Pseudomonas and Frateuria were present as indigenous KSBs in the soil. The biofertiliser applied as F. auranta solubilised 7.4 mg⋅K⋅l−1 (6.2 mg⋅K⋅kg−1) from soil minerals. The integrated K management package significantly (p < 0.001) increased the above ground biomass and leaf area of potted apple bananas by up to 57.5% compared to no input. The Study recommends an economic analysis study to determine the integrated K management package that would suit the resource constrained smallholder farmers.

Leaf Gas Exchange,Photon Capture and Light Harvest in Aldina heterophylla along a Vegetation Gradient in the Amazon Rainforest

Adaptation along environmental gradients is presumed to induce physiological and biochemical leaf changes in plant species. In this paper, we report how leaf gas exchange, photon capture and light harvest for photosynthesis in Aldina heterophylla change along a vegetation gradient from low stature open vegetation on extremely nutrient-poor white sand (Campina, CP), through inter-mediate closet type (Campinarana, CR) to tall closed rain forest (RF). The pigment concentrations did not differ between the CP, CR and RF habitats. The performance index for the photosynthesis (PIABS) of individuals in RF and CP was approximately 30% higher than that in CR individuals. This species showed similar potential rates of photosynthesis in the different vegetation types;however, the dark respiration rates were higher in CP. Our results indicate that the differences in the leaves and soil nitrogen concentrations are not enough to change the levels of gas exchange. Other environmental features may be driving the observed morphological features in this gradient, in particular, the tree height.

Co-Production of Lipids, Eicosapentaenoic Acid, Fucoxanthin, and Chrysolaminarin by Phaeodactylum tricornutum Cultured in a Flat-Plate Photobioreactor Under Varying Nitrogen Conditions

The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L^(-1)(high nitrogen, HN) and 2.9 m mol L^(-1)(low nitrogen, LN) of KNO_3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass(4.1 g L^(-1)). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid(TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids(NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L^(-1)d^(-1) was achieved in the HN group(on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase(on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L^(-1)d^(-1), respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions.

Effects of Caragana microphylla plantations on organic carbon sequestration in total and labile soil organic carbon fractions in the Horqin Sandy Land, northern China

Afforestation is conducive to soil carbon（C） sequestration in semi-arid regions. However, little is known about the effects of afforestation on sequestrations of total and labile soil organic carbon（SOC） fractions in semi-arid sandy lands. In the present study, we examined the effects of Caragana microphylla Lam. plantations with different ages（12-and 25-year-old） on sequestrations of total SOC as well as labile SOC fractions such as light fraction organic carbon（LFOC） and microbial biomass carbon（MBC）. The analyzed samples were taken from soil depths of 0–5 and 5–15 cm under two shrub-related scenarios： under shrubs and between shrubs with moving sand dunes as control sites in the Horqin Sandy Land of northern China. The results showed that the concentrations and storages of total SOC at soil depths of 0–5 and 5–15 cm were higher in 12-and 25-year-old C. microphylla plantations than in moving sand dunes（i.e., control sites）, with the highest value observed under shrubs in 25-year-old C. microphylla plantations. Furthermore, the concentrations and storages of LFOC and MBC showed similar patterns with those of total SOC at the same soil depth. The 12-year-old C. microphylla plantations had higher percentages of LFOC concentration to SOC concentration and MBC concentration to SOC concentration than the 25-year-old C. microphylla plantations and moving sand dunes at both soil depths. A significant positive correlation existed among SOC, LFOC, and MBC, implying that restoring the total and labile SOC fractions is possible by afforestation with C. microphylla shrubs in the Horqin Sandy Land. At soil depth of 0–15 cm, the accumulation rate of total SOC under shrubs was higher in young C. microphylla plantations（18.53 g C/（m^2·a）; 0–12 years） than in old C. microphylla plantations（16.24 g C/（m^2·a）; 12–25 years）, and the accumulation rates of LFOC and MBC under shrubs and between shrubs were also higher in young C. microphylla plantations than in old C. microphylla plantations. It can be concluded that the establishment of C. microphylla in the Horqin Sandy Land may be a good mitigation strategy for SOC sequestration in the surface soils.

Nutrients removal and biomass production from anaerobic digested effluent by microalgae: A review

This article summarizes the recent progress of nutrient removal from wastewater via microalgae.Removal of nitrogen and phosphorous compounds from wastewater are of great importance,while those compounds are suitable for growth of some microalgae species.Such a combination provides more opportunities for anaerobic digestion facilities,which are producing large amount of wastewater with high nitrogen and phosphorous contents.However,in order to optimize and maximize the performance and durability of the nutrient removal process,it is suggested that the basic principles about nitrogen and phosphorous migration should be investigated thoroughly,especially from the fundamentals of substance transfer mechanism between water environment and algal cells.

Carbon Isotope Discrimination is not Correlated with Transpiration Efficiency in Three Cool-Season Grain Legumes(Pulses)

The carbon isotope discrimination （δ^13C） of leaves has been shown to be correlated with the transpiration efficiency of leaves in a wide range of species. This has led to δ^13C being used in breeding programs to select for improved transpiration efficiency. The correlation between δ^13C and transpiration efficiency was determined under well-watered conditions during the vegetative phase in six genotypes of lentil （Lens culinaris Medikus）, six genotypes of chickpea （Cicerarietinum L.） and 10 cultivars of narrow-leafed lupin （Lupinus angustifolius L.）. Biomass （dry matter） accumulation and water use （transpiration） varied among the genotypes in all three species and transpiration efficiency was 40% to 75% higher in the most efficient compared with the least efficient genotypes. However, δ^13C and transpiration efficiency were not significantly correlated in any of the species. This suggests that the δ^13C technique cannot be used in selection for transpiration efficiency in the three grain legumes （pulses） studied.

Response of Growth Dynamics of Two Spring Geophytes to Light Regime in a Lime-Beech Forest

Blomaee accumulation, leaf longevity and growth rate of two spring forest geophytes, Scllla blfolla L. and Arum maculatum L. were estimated separately for three size groups within each population of these species. Despite the differences in leaf longevity, both species showed a similar pattern of blomass accumulation In relation to their phenologles and reproductive demands. Eco-physlological acclimation to changing light environment was assumed through photosynthetic parameters and dynamics of leaf area Index In the predominant size group of each species. A light response curve was measured under natural light for each species through the continuum of Its phenology to quantify the photosynthetic photon flux density at light saturation, light-saturated photosynthetic rate, light compensation point, and dark respiration. Light-saturated assimilation per leaf area basis, dark respiration rate and light compensation points were significantly higher in S. blfolla relative to A. maculatum. However, the acclimation of photosynthesis that would respond to light changes in environment was not found in S. bifolla. In contrast, In A. maculatum a marked shift In the light dependence of photosynthesis through the season was noticed, which resulted In a strong photosynthetic acclimation to the low-light conditions. Accompanied by significant leaf area Index, this efficient low-light photosynthesis enabled greater leaf longevity, and consequently longer accumulative period to A. maculatum. From the different parameters that we determined （both photosynthetic acclimation and growth strategy） it would appear that these species belong to two distinct subgroups： S. blfolla to the early and A. maculatum to the late vernals.

A biofilter model for simultaneous simulation of toluene removal and bed pressure drop under varied inlet Ioadings

In this study, a biofiltration model including the effect of biomass accumulation and inert biomass growth is developed to simultaneously predict the Volatile Organic Compounds （VOCs） removal and filter bed pressure drop tmder varied inlet loadings. A laboratoryscale experimental biofilter for gaseous toluene removal was set up and operated for 100 days with inlet toluene concentration ranging from 250 to 2500 mg· m^-2 According to sensitivity analysis based on the model, the VOCs removal efficiency of the biofilter is more sensitive to Henry＇s constant, the specific surface area of the filter bed and the thickness of water layer, while the filter bed pressure drop is more sensitive to biomass yield coefficient and original void fraction. The calculated toluene removal efficiency and bed pressure drop satisfactorily fit the experimental data under varied inlet toluene loadings, which indicates the model in this study can be used to predict VOCs removal and bed pressure drop simultaneously. Based on the model, the effect of mass-transfer parameters on VOCs removal and the stable-run time of a biofilter are analyzed. The results demonstrate that the model can function as a good tool to evaluate the effect of biomass accumulation and optimize the design and operation of biofilters.