期刊文献+
共找到7篇文章
< 1 >
每页显示 20 50 100
Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells 被引量:12
1
作者 Ping Wang Liang Zhao +3 位作者 Jason Liu Michael D Weir Xuedong Zhou Hockin H K Xu 《Bone Research》 SCIE CAS 2014年第3期139-151,共13页
Tissue engineering is promising to meet the increasing need for bone regeneration. Nanostructured calcium phosphate (CAP) biomaterials/scaffolds are of special interest as they share chemical/crystallographic simila... Tissue engineering is promising to meet the increasing need for bone regeneration. Nanostructured calcium phosphate (CAP) biomaterials/scaffolds are of special interest as they share chemical/crystallographic similarities to inorganic components of bone. Three applications of nano-CaP are discussed in this review: nanostructured calcium phosphate cement (CPC); nano-CaP composites; and nano-CaP coatings. The interactions between stem cells and nano-CaP are highlighted, including cell attachment, orientation/ morphology, differentiation and in vivo bone regeneration. Several trends can be seen: (i) nano-CaP biomaterials support stem cell attachment/proliferation and induce osteogenic differentiation, in some cases even without osteogenic supplements; (ii) the influence of nano-CaP surface patterns on cell alignment is not prominent due to non-uniform distribution of nano-crystals; (iii) nano-CaP can achieve better bone regeneration than conventional CaP biomaterials; (iv) combining stem cells with nano-CaP accelerates bone regeneration, the effect of which can be further enhanced by growth factors; and (v) cell microencapsulation in nano-CaP scaffolds is promising for bone tissue engineering. These understandings would help researchers to further uncover the underlying mechanisms and interactions in nano-CaP stem cell constructs in vitro and in vivo, tailor nano-CaP composite construct design and stem cell type selection to enhance cell function and bone regeneration, and translate laboratory findings to clinical treatments. 展开更多
关键词 CPC Bone tissue engineering via nanostructured calcium phosphate biomaterials and stem cells STEM
下载PDF
Regenerative Engineering for Knee Osteoarthritis Treatment: Biomaterials and Cell-Based Technologies 被引量:3
2
作者 Jorge L. Escobar Ivirico Maumita Bhattacharjee +2 位作者 Emmanuel Kuyinu Lakshmi S. Nair Cato T. Laurencin 《Engineering》 SCIE EI 2017年第1期16-27,共12页
Knee osteoarthritis (OA) is the most common form of arthritis worldwide. The incidence of this disease is rising and its treatment poses an economic burden. Two early targets of knee OA treatment include the predomi... Knee osteoarthritis (OA) is the most common form of arthritis worldwide. The incidence of this disease is rising and its treatment poses an economic burden. Two early targets of knee OA treatment include the predominant symptom of pain, and cartilage damage in the knee joint. Current treatments have been beneficial in treating the disease but none is as effective as total knee arthroplasty (TKA). However, while TKA is an end-stage solution of the disease, it is an invasive and expensive procedure, Therefore, innovative regenerative engineering strategies should be established as these could defer or annul the need for a TKA. Several biomaterial and cell-based therapies are currently in development and have shown early promise in both preclinical and clinical studies. The use of advanced biomaterials and stem cells independently or in conjunction to treat knee OA could potentially reduce pain and regenerate fo- cal articular cartilage damage. In this review, we discuss the pathogenesis of pain and cartilage damage in knee OA and explore novel treatment options currently being studied, along with some of their limitations. 展开更多
关键词 Knee osteoarthritis Osteoarthritic pain Mesenchymal stem cells biomaterials Regenerative engineering
下载PDF
Extracellular matrix from human umbilical cordderived mesenchymal stem cells as a scaffold for peripheral nerve regeneration 被引量:8
3
作者 Bo Xiao Feng Rao +10 位作者 Zhi-yuan Guo Xun Sun Yi-guo Wang Shu-yun Liu Ai-yuan Wang Quan-yi Guo Hao-ye Meng Qing Zhao Jiang Peng Yu Wang Shi-bi Lu 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第7期1172-1179,共8页
The extracellular matrix,which includes collagens,laminin,or fibronectin,plays an important role in peripheral nerve regeneration.Recently,a Schwann cell-derived extracellular matrix with classical biomaterial was use... The extracellular matrix,which includes collagens,laminin,or fibronectin,plays an important role in peripheral nerve regeneration.Recently,a Schwann cell-derived extracellular matrix with classical biomaterial was used to mimic the neural niche.However,extensive clinical use of Schwann cells remains limited because of the limited origin,loss of an autologous nerve,and extended in vitro culture times.In the present study,human umbilical cord-derived mesenchymal stem cells(h UCMSCs),which are easily accessible and more proliferative than Schwann cells,were used to prepare an extracellular matrix.We identified the morphology and function of h UCMSCs and investigated their effect on peripheral nerve regeneration.Compared with a non-coated dish tissue culture,the h UCMSC-derived extracellular matrix enhanced Schwann cell proliferation,upregulated gene and protein expression levels of brain-derived neurotrophic factor,glial cell-derived neurotrophic factor,and vascular endothelial growth factor in Schwann cells,and enhanced neurite outgrowth from dorsal root ganglion neurons.These findings suggest that the h UCMSC-derived extracellular matrix promotes peripheral nerve repair and can be used as a basis for the rational design of engineered neural niches. 展开更多
关键词 umbilical Schwann regeneration scaffold neurotrophic biomaterial niche fibronectin glial engineered
下载PDF
In vitro Characterization of PBLG-g-HA/PLLA Nanocomposite Scaffolds 被引量:1
4
作者 廖岚 YANG Shuang +3 位作者 Richard J Miron WEI Junchao ZHANG Yufeng 张萌 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2014年第4期841-847,共7页
The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vit... The purpose of the present study was to synthesize a new composites scaffold containing poly(γ-benzyl-L-glutamate) modified hydroxyapatite/(poly(L-lactic acid))(PBLG-g-HA/PLLA) and to investigate their in vitro behaviour on bone mesenchymal stromal cells(BMSCs). The results demonstrated that BMSC proliferation was signifi cantly increased on PBLG-g-HA/PLLA scaffolds after 3 and 7 days post seeding when compared to PLLA and HA/PLLA scaffolds. The in vitro osteogenic differentiation also favoured the composite PBLG-g-HA/PLLA scaffolds when compared to controls by signifi cantly increasing Runx2, ALP or osteocalcin mRNA expression as assessed by real-time PCR. The results illustrate the potential of PBLG-g-HA/PLLA scaffolds for bone tissue engineering applications. And the in vivo testing further confi rms the PBLG-gHA/PLLA scaffolds' potentioal for healing critical bone defects. 展开更多
关键词 hydroxyapatite poly(L-lactide) tissue engineering biomaterials
下载PDF
Modelling the development of biological structures displaying longitudinal geometries in vitro:culturing pluripotent stem cells on plasma-treated,growth factor-coupled polycaprolactone fibres
5
作者 Badwi B.Boumelhem Stuart T.Fraser +7 位作者 Syamak Farajikhah Rachel A.Shparberg Michael B.Morris Marcela M.M.Bilek Anyu Zhang Behnam Akhavan Simon Fleming Maryanne Large 《Engineered Regeneration》 EI 2024年第1期124-138,共15页
Many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear axes.Model... Many biological structures such as nerves,blood and lymphatic vessels,and muscle fibres exhibit longitudinal ge-ometries with distinct cell types extending along both the length and width of internal linear axes.Modelling these three-dimensional structures in vitro is challenging:the best-defined stem-cell differentiation systems are mono-layer cultures or organoids using pluripotent stem cells.Pluripotent stem cells can differentiate into functionally mature cells depending on the signals received,holding great promise for regenerative medicine.However,the integration of in vitro differentiated cell types into diseased tissue remains a challenge.Engineered scaffolds can bridge this gap if the appropriate signalling systems are incorporated into the scaffold.Here,we have taken a biomimicry approach to generate longitudinal structures in vitro.In this approach,mouse embryonic stem cells are directed to differentiate to specific cell types on the surface of polycaprolactone(PCL)fibres treated by plasma-immersion ion implantation and to which with lineage-specifying molecules have been covalently im-mobilised.We demonstrate the simplicity and utility of our method for efficiently generating high yields of the following cell types from these pluripotent stem cells:neurons,vascular endothelial cells,osteoclasts,adipocytes,and cells of the erythroid,myeloid,and lymphoid lineages.Strategically arranged plasma-treated scaffolds with differentiated cell types could ultimately serve as a means for the repair or treatment of diseased or damaged tissue. 展开更多
关键词 Pluripotent stem cells Lineage commitment Growth factors Biomaterial engineering Plasma-immersion ion implantation Covalent biomolecule attachment POLYCAPROLACTONE
原文传递
Biomedical applications of magneto-responsive scaffolds 被引量:3
6
作者 Adedokun A. Adedoyin Adam K. Ekenseair 《Nano Research》 SCIE EI CAS CSCD 2018年第10期5049-5064,共16页
Stimuli-responsive biomaterials, capable of responding on-demand to changes in their local environment, have become a subject of interest in the field of regenerative medicine. Magneto-responsive biomaterials, which c... Stimuli-responsive biomaterials, capable of responding on-demand to changes in their local environment, have become a subject of interest in the field of regenerative medicine. Magneto-responsive biomaterials, which can be manipulated spatiotemporally via an external magnetic field, have emerged as promising candidates as active scaffolds for advanced drug delivery and tissue regeneration applications. These specialized biomaterials can be synthesized by physically and/or chemically incorporating magnetic nanoparticles into the biomaterial structure. However, despite their promising impact on the future of regenerative medicine, magneto-responsive biomaterials still have several limitations that need to be overcome before they can be implemented clinically in a reliable manner, as predicting their behavior and biocompatibility remains an ongoing challenge. This review article will focus on discussing the current fabrication methods used to synthesize magneto-responsive materials, efforts to predict and characterize magneto-responsive biomaterial behavior, and the application of magneto-responsive biomaterials as controlled drug delivery systems, tissue engineering scaffolds, and artificial muscles. 展开更多
关键词 biomaterials magnetic nanoparticles magneto-responsive scaffolds regenerative medicine stimuli-responsive materials tissue engineering biomaterials magnetic nanoparticles magneto-responsivescaffolds regenerative medicine stimuli-responsivematerials tissue engineering
原文传递
Preparation and Characterization of Hydroxyapatite-Coated Selenium Nanoparticles and their Interaction with Osteosarcoma(SaOS-2) Cells 被引量:4
7
作者 Thiagarajan Hemalatha Gunasekaran Krithiga +1 位作者 Baskar Santhosh Kumar Thotapalli P. Sastry 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2014年第6期1152-1158,共7页
Nanotechnology has taken a firm step to revolutionize the field of orthopedic implants. Current research on bone implants focuses to develop implants with multifaceted functions viz., osteoinduction, chemoprevention, ... Nanotechnology has taken a firm step to revolutionize the field of orthopedic implants. Current research on bone implants focuses to develop implants with multifaceted functions viz., osteoinduction, chemoprevention, antimicrobial action etc., especially for cancerous bone resection. The objective of the present study was to synthesize a novel composite for bone implants, possessing the above properties. Selenium was selected owing to its chemopreventive and chemotherapeutic properties. Hydroxyapatite was selected owing to its bioactivity and similarity in composition to bone mineral properties. Selenium nanoparticles were prepared by chemical reduction method and coated with hydroxyapatite.Hydroxyapatite-coated selenium nanoparticle(HASnp) was characterized physico-chemically using fourier transform infrared spectroscopy, X-ray diffractometry, scanning electron microscope, and energy-dispersive X-ray spectroscopy.HASnp was analysed in vitro using SaOS-2 cell line. Enhanced cell proliferation and alkaline phosphatase activity were observed in HASnp-treated cells. The results indicate that HASnp is highly suitable for the use in orthopedic applications. 展开更多
关键词 Osteogenesis Biocompatibility Bone tissue engineering biomaterials Biomedical applications
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部