Direct Electron Communication Between Glucose Oxidase and Carbon Electrode Covered with Polypyrrole

Glucose oxidase can be effectively adsorbed onto the polypyrrole (PPy) thin film electrochemically formed on an anodized galssy carbon electrode (GCEa ). Direct electron communication between the redox of GOD and the modified electrode was successfully achieved, which was detected using cyclic voltammetry. GOD entrapped in PPy film still remained its biological activity and could catalyze the oxidation of glucose. As a third generation biosensor, GOD-PPy/GCEa responded linearly up to 20 mM glucose with a wider linear concentration range.

基于镀铂碳糊电极电聚酪胺和过氧化聚吡咯的葡萄糖传感器研究

用镀铂碳糊电极电聚酪胺和过氧化聚毗咯固定葡萄糖氧化酶（GOD）制成电流型葡萄糖传感器。铂黑沉积层的微孔结构使电极具有较大的有效表面积，增加了固定的酶量，电极灵敏度提高。通过聚合酪胺（PTY）和过氧化聚吡咯（OPPY），可提高酶电极的稳定性和抗干扰能力。该电极具有响应时间短（〈10s）、响应范围宽（0.01—18mmol／L）、灵敏度高（检出限为5μmol／L）、稳定性好等优点，电极经过60d、约60次反复测试后，响应电流下降至最初值的70％，并具有抗果糖、尿酸和抗坏血酸干扰的特点。

电导聚合物修饰葡萄糖氧化酶电极研究

采用分步聚合方法,制备以聚吡咯膜为载体的葡萄糖氧化酶电极,探讨该电极的生物电化学响应特性和实用性,以及电位、pH值、温度和浓度等对电极响应的影响.实验表明,该电极的线性响应范围为(1.0×10-6～2.0×10-2)mol/L,响应时间为9 s,具有良好的重现性和高选择性,且可显著提高酶电极的耐温能力.