This study explored the factors influencing cooperative innovation in environmentally friendly agricultural biotechnology in China.First,we constructed an evolutionary game model comprising the factors of net income o...This study explored the factors influencing cooperative innovation in environmentally friendly agricultural biotechnology in China.First,we constructed an evolutionary game model comprising the factors of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies.Using MATLAB simulation,we assigned different values to the aforementioned variables to explore the evolutionary trend of innovators’willingness to cooperate.Results showed that when the values of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies exceeded the threshold,innovators’willingness to cooperate was significantly enhanced.Furthermore,the proportion of innovators who cooperated with others gradually increased to 100%;otherwise,it gradually decreased to 0%.Comparing the simulation curve with the real evolution curve of cooperative innovation in agricultural biotechnology in China,we found that the gradual decline in the willingness to cooperate could be due to insufficient subsidies for cooperative innovation,low income from cooperative innovation,weak profitability of innovators,and weak market constraints.展开更多
Through the online and offline practical reform of the Modern Instrumental Analysis course of biotechnology majors from teaching content,teaching method,teaching demonstration to teaching effect,the traditional single...Through the online and offline practical reform of the Modern Instrumental Analysis course of biotechnology majors from teaching content,teaching method,teaching demonstration to teaching effect,the traditional single offline lecture is transformed into diversified and interactive modern teaching.The practical reform enriches and optimizes the course content,perfects and improves the course assessment system,and improves the teaching quality.It achieves the student-centered and application-oriented teaching goal,and also provides reference for further cultivating high-quality applied talents.展开更多
Kappaphycus alvarezii(K.alvarezii)is a perennial large algae native to tropical and subtropical regions.With a slender thallus,it branches better at the upper part than the lower end.The sea moss is mainly found in Ha...Kappaphycus alvarezii(K.alvarezii)is a perennial large algae native to tropical and subtropical regions.With a slender thallus,it branches better at the upper part than the lower end.The sea moss is mainly found in Hainan Province,China,and Southeast Asian countries such as Malaysia and the Philippines because it grows best in the subtidal zone of clean.展开更多
α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from d...α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA content.Biotechnological enrichment of ALA in staple oil crops is a promising strategy.Chia(Salvia hispanica)has the highest ALA content in its seed oil among known oil crops.In this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),respectively.The highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective controls.FA-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly up-regulated.In contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly down-regulated.This means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic B.napus seeds.展开更多
经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原...经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原有双语课程教学的基础上,通过优化教材和教学内容,打造双向教学模式和建设多元评价体系并持续改进,构建了多层次启发式、互动、研讨相结合的复合教学体系,显著增强留学生专业基础知识水平和自主学习能力,为后续课程学习和研究工作的开展奠定了良好的基础。教学实践结果表明,该教学体系对提高该类课程的学习成效和课程质量,具有较为显著的示范效应。展开更多
基金funded by National Social Science Fund the Evolution of Japan’s Food Security Policy and Its Enlightenment to China[Grant No.22CSS016].
文摘This study explored the factors influencing cooperative innovation in environmentally friendly agricultural biotechnology in China.First,we constructed an evolutionary game model comprising the factors of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies.Using MATLAB simulation,we assigned different values to the aforementioned variables to explore the evolutionary trend of innovators’willingness to cooperate.Results showed that when the values of net income of cooperative innovation,net income of independent innovation,market constraints,and government subsidies exceeded the threshold,innovators’willingness to cooperate was significantly enhanced.Furthermore,the proportion of innovators who cooperated with others gradually increased to 100%;otherwise,it gradually decreased to 0%.Comparing the simulation curve with the real evolution curve of cooperative innovation in agricultural biotechnology in China,we found that the gradual decline in the willingness to cooperate could be due to insufficient subsidies for cooperative innovation,low income from cooperative innovation,weak profitability of innovators,and weak market constraints.
基金Supported Biotechnology Application Demonstration Major in Hebei Province(20801001002)Provincial Biotechnology Application Demonstration Major(SYLZY2021-1)+2 种基金School-level Biotechnology Application Demonstration Major(XYYZY2024-1)School-level Education and Teaching Reform Project of Langfang Normal University in 2022(K2022-22)Industry-University Cooperative Education Project of Ministry of Education(20210211904).
文摘Through the online and offline practical reform of the Modern Instrumental Analysis course of biotechnology majors from teaching content,teaching method,teaching demonstration to teaching effect,the traditional single offline lecture is transformed into diversified and interactive modern teaching.The practical reform enriches and optimizes the course content,perfects and improves the course assessment system,and improves the teaching quality.It achieves the student-centered and application-oriented teaching goal,and also provides reference for further cultivating high-quality applied talents.
文摘Kappaphycus alvarezii(K.alvarezii)is a perennial large algae native to tropical and subtropical regions.With a slender thallus,it branches better at the upper part than the lower end.The sea moss is mainly found in Hainan Province,China,and Southeast Asian countries such as Malaysia and the Philippines because it grows best in the subtidal zone of clean.
基金This work was supported by the National Natural Science Foundation of China(31871549,32001441 and 32272015)the Chongqing Research Program of Basic Research and Frontier Technology,China(cstc2015jcyjBX0143)+2 种基金the Fundamental Research Funds for the Central Universities,China(XDJK2020C038)the National Key R&D Program of China(2016YFD0100506)the Young Eagles Program of Chongqing Municipal Commission of Education,China(CY220219)。
文摘α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA content.Biotechnological enrichment of ALA in staple oil crops is a promising strategy.Chia(Salvia hispanica)has the highest ALA content in its seed oil among known oil crops.In this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),respectively.The highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective controls.FA-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly up-regulated.In contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly down-regulated.This means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic B.napus seeds.
文摘经过十多年的建设,"Essentials of Biotechnology"已建设成为江南大学乃至江苏省来华留学生英语授课品牌课程。该课程针对来华留学生学科背景有较大差异,英语能力参差不齐和课程知识涉及面广,知识点较多,理论性强等问题,在原有双语课程教学的基础上,通过优化教材和教学内容,打造双向教学模式和建设多元评价体系并持续改进,构建了多层次启发式、互动、研讨相结合的复合教学体系,显著增强留学生专业基础知识水平和自主学习能力,为后续课程学习和研究工作的开展奠定了良好的基础。教学实践结果表明,该教学体系对提高该类课程的学习成效和课程质量,具有较为显著的示范效应。
