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口蹄疫病毒P12A-3C免疫原基因在百脉根中的遗传转化与表达 被引量:16
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作者 王炜 张永光 +9 位作者 潘丽 王永录 方玉珍 蒋守田 张维德 吕建亮 孙元 智晓莹 黄振华 刘力宽 《中国人兽共患病学报》 CAS CSCD 北大核心 2007年第3期236-239,247,共5页
目的利用植物反应器研究口蹄疫转基因植物疫苗是近些年来科学家研究的热点,本研究以豆科牧草百脉根为转化受体,将口蹄疫病毒P12A-3C基因通过根癌农杆菌介导法导入百脉根基因组。方法百脉根外植体经过浸菌,抗性培养基上愈伤、出芽和生根... 目的利用植物反应器研究口蹄疫转基因植物疫苗是近些年来科学家研究的热点,本研究以豆科牧草百脉根为转化受体,将口蹄疫病毒P12A-3C基因通过根癌农杆菌介导法导入百脉根基因组。方法百脉根外植体经过浸菌,抗性培养基上愈伤、出芽和生根等阶段。结果最终获得了转基因抗性植株。结论对转基因植株进行PCR、RT-PCR检测,表明外源基因整合在植物染色体基因组,并且具有转录活性,ELISA检测表明,转基因植株表达出外源目的蛋白。 展开更多
关键词 口蹄疫 百脉根 转基因植物 疫苗
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低量施肥对高燕麦草草地植被及绵羊择食行为的影响 被引量:7
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作者 王明玖 HutchinsonGK 《草业学报》 CSCD 1997年第4期24-30,共7页
于1995年8~11月在新西兰TaraHils山地草地试验站进行草地施肥和放牧研究。结果表明,在所有小区的施肥区域,绵羊的分布比例都显著多于非施肥区域(P<0.005)。通过复相关分析,发现这种分布由于施肥量、建群植... 于1995年8~11月在新西兰TaraHils山地草地试验站进行草地施肥和放牧研究。结果表明,在所有小区的施肥区域,绵羊的分布比例都显著多于非施肥区域(P<0.005)。通过复相关分析,发现这种分布由于施肥量、建群植物、观测日期和观测时间的不同而略有差异,而这又与施肥促进豆科牧草的生长(主要是兔足三叶草Trifoliumarvense)、提高高燕麦草的适口性、提高草群的含水量等有直接关系。 展开更多
关键词 高燕麦草 百脉根 低量施肥 绵羊择食行为 草地
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Reproductive Development of Lotus tenuis (Fabaceae) Crop Defoliated at Different Times and Intensities
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作者 Osvaldo R. Vignolio Gustavo S. Cambareri +2 位作者 Lucas R. Petigrosso Natalia Murillo Néstor O. Maceira 《American Journal of Plant Sciences》 2016年第8期1180-1191,共12页
Lotus tenuis forage yield has been quantified under defoliation conditions in pastures, grasslands and under dual-purpose production of both livestock forage and seeds. However, little is known about the effects of de... Lotus tenuis forage yield has been quantified under defoliation conditions in pastures, grasslands and under dual-purpose production of both livestock forage and seeds. However, little is known about the effects of defoliation management on L. tenuis flower and pod production and subsequent seed yield. Two field experiments were conducted to study the response of L. tenuis to defoliation at different flowering stages and intensities. In Experiment 1, crops were defoliated at the beginning of the flowering (DBF), mid-flowering (DMF) or full flowering (DFF). In Experiment 2, defoliation was in vegetative stage at low (LDI) or high (HDI) intensities. Defoliation in Experiment 1 neither affected plant cover nor the photosynthetically active radiation intercepted by the crop during pod production. There were less umbels with dehiscent (shattered) pods in the DFF treatment than in Control, DBF and DMF treatments. Flower peak occurred first in the Control, DBF and DMF treatments, and eight days later in DFF plots, however, seed yield was not affected (1324 ± 32.8 kg·ha<sup>-1</sup>). Defoliation intensity did not affect seed yield (962 ± 25.9 kg·ha<sup>-</sup><sup>1</sup>) because of self-compensation which increased harvest index in HDI (14.5% ± 0.6%) compared to the Control and LDI (12.0% ± 0.3%) treatments. Plant survival was not affected by defoliation treatments in any of the experiments. Flowering can be synchronized through defoliation. The blooming of large numbers of flowers in a short time was achieved, reducing the number of shattered pods. Compensatory responses through plant plasticity conferred L. tenuis the ability to overcome defoliation without affecting seed yield. Lotus tenuis defoliation as management tool will be considered in future researches because it is possible to harvest forage and to increase seed yield through a reduction of shattered pods. 展开更多
关键词 CUTTING Narrowleaf birdsfoot trefoil Vegetative Biomass Flowering Time Yield
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补播百脉根改良低湿草地技术研究 被引量:7
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作者 姬宏亮 蔡东萍 《草食家畜》 2006年第3期61-64,共4页
该课题利用草原退化理论、改良理论,借鉴新疆畜牧科学院草原研究所在阿勒泰补播百脉根改良草场技术、新疆农业科学院农业机械化研究所研制的草原松土补播机,大面积补播百脉根改良草原,和对照组相比,补播组在植被高度、盖度、密度和产草... 该课题利用草原退化理论、改良理论,借鉴新疆畜牧科学院草原研究所在阿勒泰补播百脉根改良草场技术、新疆农业科学院农业机械化研究所研制的草原松土补播机,大面积补播百脉根改良草原,和对照组相比,补播组在植被高度、盖度、密度和产草量方面有显著增加,土壤理化性质方面得到改善,经济和生态效益显著,为我区草原改良,特别是低湿草地的改良起到借鉴作用。 展开更多
关键词 百脉根 补播 改良 试验 草原退化理论
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Establishing Legumes in a Tall Fescue Sward
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作者 Doo-Hong Min Joseph L. Moyer 《American Journal of Plant Sciences》 2015年第2期355-361,共7页
Tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.] is a common grass species in the eastern half of the USA, but legumes grown with it could provide benefits. Obstacles to legume establishment in fescue pastures... Tall fescue [Schedonorus arundinaceus (Schreb.) Dumort.] is a common grass species in the eastern half of the USA, but legumes grown with it could provide benefits. Obstacles to legume establishment in fescue pastures include disease, insect damage, and grass competition. Experiments were performed in 2010 and 2011 at Site 1 to test the efficacy of insect control, disease control, and two methods of grass suppression on seedling establishment of three legumes. The highest seedling density of red clover (Trifolium pretense L.) was obtained with glyphosate treatment regardless of pest control and for clipping without pest control. No overall or consistent benefit was found for white clover (Trifolium pretense L.) or trefoil (Lotus corniculatus L.) from seed and foliar insecticides or fungicides, or for grass suppression by either clipping or glyphosate application. Experiments were performed in 2012 and 2013 at Site 2 to test the efficacy of grass suppression by grazing cattle or by clethodim application. Seedling density of red clover was not significantly improved by either treatment, but the density of birdsfoot trefoil was increased by clethodim, and the density of white clover was increased in 2013 by both methods of grass suppression. Overall, red clover was least sensitive to grass competition. Birdsfoot trefoil was better served by the immediate effect of chemical suppression, whereas white clover benefitted most from the lengthier grass suppression provided by grazing. 展开更多
关键词 RED CLOVER WHITE (Ladino) CLOVER birdsfoot trefoil HERBICIDE GRAZING
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