With annual Bischofia javanica seedlings as experimental material, the plasticity and comprehensive evaluation methods were employed to investigate the effects of different shading degrees （100% NS （natural sunshine...With annual Bischofia javanica seedlings as experimental material, the plasticity and comprehensive evaluation methods were employed to investigate the effects of different shading degrees （100% NS （natural sunshine）, 41.3% NS, 14.6% NS and 3.6% NS） on B. javanica seedlings growth, so as to obtain the shade toler- ance in B. javanica. The results showed that with the shading degree increasing, the seedling height increment, diameter increment, leaf area, fresh weights （above- ground part, underground part and whole plant） and dry weights （aboveground part, underground part and whole plant） of B. javanica seedlings all increased first and then decreased, and reached peak values under 41.3% NS, while the total root length, average root diameter, total surface area, total root volume and total root number all presented a decreasing trend. Also the comprehensive evaluation by subordinate function value method and plasticity analysis were carried out on these growth indexes. It was concluded that B. javanica seedlings have medium shade tol- erance from the comDrehensive analysis of growth indexes.展开更多
Objective: Sarcopenia causes loss of skeletal muscle and function, thus seriously affecting the physical function and quality of life in the elderly. This article discusses the specific molecular mechanism and amelior...Objective: Sarcopenia causes loss of skeletal muscle and function, thus seriously affecting the physical function and quality of life in the elderly. This article discusses the specific molecular mechanism and ameliorating effects of Tudangshen(TDS) on sarcopenia in elderly rats with type 2 diabetes mellitus(T2DM).Methods: Elderly Sprague-Dawley(SD) rats were randomly selected and fed with a high-fat diet combined with an intraperitoneal injection of streptozotocin to establish T2DM model. The model rats were stratified and randomly divided into the model group,metformin group, TDS high-dose group, TDS medium-dose group, and TDS low-dose group according to blood glucose combined with body weight, and the same batch of old SD rats was set as the normal control group. The effects of TDS in an elderly T2DM sarcopenia rat model were evaluated by observing the body positions of the rats, analyzing blood biochemistry, testing exercise capacity, and pathologically staining sectioned gastrocnemius muscle tissues. The molecular mechanisms of the effects were analyzed using quantitative real-time polymerase chain reaction and western blotting.Results: TDS has no statistically significant effect on blood glucose, insulin and glycosylated serum protein in aged rats with T2DM, but it can reduce levels of glycosylated serum protein, total cholesterol, triglycerides, and low-density lipoprotein;it improves pathological changes in rat gastrocnemius muscle tissues, and increases muscle cell activity in elderly rats with T2DM and sarcopenia. TDS also promoted the upregulation of the expression of mammalian target of rapamycin(mTOR)/protein kinase B(PKB/AKT)/phosphatidylinositol 3-kinase(PI3K)/ribosomal protein S6 kinase/eukaryotic initiation factor 4 E binding rotein1 mRNA in rats and triggered an increase in corresponding protein levels.Conclusions: TDS alleviated muscle decline in elderly rats with T2DM by activating the PI3 K/AKT/mTOR signaling pathway and regulating the synthesis of corresponding proteins.展开更多
文摘With annual Bischofia javanica seedlings as experimental material, the plasticity and comprehensive evaluation methods were employed to investigate the effects of different shading degrees （100% NS （natural sunshine）, 41.3% NS, 14.6% NS and 3.6% NS） on B. javanica seedlings growth, so as to obtain the shade toler- ance in B. javanica. The results showed that with the shading degree increasing, the seedling height increment, diameter increment, leaf area, fresh weights （above- ground part, underground part and whole plant） and dry weights （aboveground part, underground part and whole plant） of B. javanica seedlings all increased first and then decreased, and reached peak values under 41.3% NS, while the total root length, average root diameter, total surface area, total root volume and total root number all presented a decreasing trend. Also the comprehensive evaluation by subordinate function value method and plasticity analysis were carried out on these growth indexes. It was concluded that B. javanica seedlings have medium shade tol- erance from the comDrehensive analysis of growth indexes.
基金supported by the National Natural Science Foundation of China(Grant No.:82074394)。
文摘Objective: Sarcopenia causes loss of skeletal muscle and function, thus seriously affecting the physical function and quality of life in the elderly. This article discusses the specific molecular mechanism and ameliorating effects of Tudangshen(TDS) on sarcopenia in elderly rats with type 2 diabetes mellitus(T2DM).Methods: Elderly Sprague-Dawley(SD) rats were randomly selected and fed with a high-fat diet combined with an intraperitoneal injection of streptozotocin to establish T2DM model. The model rats were stratified and randomly divided into the model group,metformin group, TDS high-dose group, TDS medium-dose group, and TDS low-dose group according to blood glucose combined with body weight, and the same batch of old SD rats was set as the normal control group. The effects of TDS in an elderly T2DM sarcopenia rat model were evaluated by observing the body positions of the rats, analyzing blood biochemistry, testing exercise capacity, and pathologically staining sectioned gastrocnemius muscle tissues. The molecular mechanisms of the effects were analyzed using quantitative real-time polymerase chain reaction and western blotting.Results: TDS has no statistically significant effect on blood glucose, insulin and glycosylated serum protein in aged rats with T2DM, but it can reduce levels of glycosylated serum protein, total cholesterol, triglycerides, and low-density lipoprotein;it improves pathological changes in rat gastrocnemius muscle tissues, and increases muscle cell activity in elderly rats with T2DM and sarcopenia. TDS also promoted the upregulation of the expression of mammalian target of rapamycin(mTOR)/protein kinase B(PKB/AKT)/phosphatidylinositol 3-kinase(PI3K)/ribosomal protein S6 kinase/eukaryotic initiation factor 4 E binding rotein1 mRNA in rats and triggered an increase in corresponding protein levels.Conclusions: TDS alleviated muscle decline in elderly rats with T2DM by activating the PI3 K/AKT/mTOR signaling pathway and regulating the synthesis of corresponding proteins.
