It is of great significance to systematically analyze the cultivated land system resilience(CLSR) for the black soil protection and national food security.The CLSR is impacted by planting structure adjustment and cult...It is of great significance to systematically analyze the cultivated land system resilience(CLSR) for the black soil protection and national food security.The CLSR is impacted by planting structure adjustment and cultivated land quality decline,posing major hidden dangers to food security.It is urgent to evaluate the CLSR at multiple spatio-temporal scales.This study took Liaoning Province in the black soil region of Northeast China as an example.Based on the resilience theory,this study constructed the CLSR evaluation system from the input-feedback perspective at the provincial-scale and the city-scale,and used the rank-sum ratio comprehensive evaluation method(RSR) to analyze the key influencing factors of CLSR in Liaoning Province and its 14 cities from 2000 to 2019.The results showed that:1) the time series changes of CLSR at the provincial-scale and the city-scale in Liaoning Province were similar,both showing an increasing trend.2) The CLSR in Liaoning Province presented a spatial pattern of ‘high in the west and low in the east’ at the city-scale.3) There were seven and six main influencing factors of CLSR at the provincial-scale and the city-scale,respectively.In addition to the net income per capita of rural households,other influencing factors of CLSR were different at the provincial-scale and the city-scale.The feedback factors were dominant at the provincial-scale,and the input factors and feedback factors were dominant at the city-scale.The results could provide a reference for the utilization of black soil and draw on the experience of regional agricultural planning and adjustment.展开更多
[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the ap...[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.展开更多
As the population continues to shrink in the black soil region of Northeast China since 2000,it is critical to master the impact of population shrinkage on rural functions to realize rural revitalization and sustainab...As the population continues to shrink in the black soil region of Northeast China since 2000,it is critical to master the impact of population shrinkage on rural functions to realize rural revitalization and sustainable development.In this study,we focused on the impacts of depopulation on the evolution and interrelationship of rural subfunctions.Based on the rural function indexes system,the TOPSIS(Technique for Order Preference by Similarity to an Ideal Solution)method,spatial analysis method,and mathematical statistics analysis method were used to summarize the spatial and temporal characteristics of rural function development,as well as the effect of population shrinkage in the typical black soil region of Northeast China.The results showed that depopulation varied in the extent and duration between the forested region and plain areas,which both impacted the trajectories of rural subfunctions evolution.For the economic development function and ecological conservation function,the effect of continuous slight depopulation was beneficial,while the effect of rapid depopulation was adverse,which was exactly opposite to the agricultural production function.All forms of population shrinkage were conducive to the development of the social security function.With the deepening population shrinkage,depopulation mainly promoted the collaborative development between subfunctions in this study,except the relationship between agricultural production and social security function.But effects of depopulation on the interrelationship of rural subfunctions varied between the forested region and plain areas in some cases.The results provided evidence for the cognition that population shrinkage had complicated effects on rural subfunctions.展开更多
Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this st...Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this study,320 counties in the black soil region of Northeast China(BSRNC)represent the research units used to construct the CQE system measuring the soil properties(SP),cultivated land productivity(CLP),ecological environment(EE)and social economy(SE).The total of 19 factors were selected to calculate the integrated fertility index(IFI)and divided into grades.Simultaneously,we used the coupling coordination degree model to comprehensively analyze the spatial pattern of the cultivated land quality(CLQ)in the BSRNC,and use the structural equation model(SEM)to analyze the driving mechanism.The results show the following:1)The CLQ of 262 counties in the BSRNC is in a state of coupling and coordination,and the coupling and coordination degree presents a spatial distribution pattern of‘high in the southwest and northeast,low in the northwest and southeast’.The coordinated development degree of 271 counties is between 0.4 and 0.6,which is in a transitional state between coordination and disorder.2)The CLQ in the BSRNC is generally good,with an average grade of 3.High-quality cultivated land accounts for 58.45%of all counties,middle-and upper-quality cultivated land accounts for 27.05%,and poor-quality cultivated land accounts for 14.49%.3)The SEM analysis shows that the SP,CLP,EE,and SE all influence the CLQ.Among them,the SP has the largest driving force on the CLQ,while the SE has the smallest driving force on it.The results confirm that the main factors affecting the evaluation results are crop productivity level,normalized difference vegetation index,ratio vegetation index,difference vegetation index,and organic carbon content.When implementing protection measures in counties with a low CLQ,considering a balanced coordination of multiple systems and reasonably controlling the quality degradation are important.This study provides the current situation and driving factors of the CLQ in the BSRNC and will play an important role in black soil governance and utilization.展开更多
The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security ...The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this region.In this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis methods.The results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late 2000s.CSp of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in April.For the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,respectively.The rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after 1984.The rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation characteristics.The spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast China.Compared with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has increased.The trends of seasonal and monthly C in 82.2%~87.7%of the stations show significant decreases at 95%confidence level.CSp is closely related to wind speed at 2m height,temperature difference,minimum temperature and precipitation in the same period,of which the correlation between CSp and wind speed is the strongest,indicating that the main control factor for CSp in the study area is wind speed,but the impact of the change of temperature and precipitation on CSp cannot be ignored.展开更多
It is of great significance to study the influence of spatial pattern change of cultivated land on eco-system service,including the sustainable utilization and ecological protection in black soil region in Northeast C...It is of great significance to study the influence of spatial pattern change of cultivated land on eco-system service,including the sustainable utilization and ecological protection in black soil region in Northeast China.Taking Lishu County of Jilin Province as the study area,we quantitatively evaluated soil conservation,habitat quality,carbon storage and grain production service,and analyzed the change of cultivated land and ecosystem service pattern in Lishu County from 1990 to 2020 by using Pearson correlation analysis on the correlation between cultivated land change and ecosystem service.The results show that:(1)The cultivated land area of Lishu County was reduced by 19.67 km2,the characteristics of cultivated land change are signifi-cant;(2)the overall change rate of carbon storage,habitat quality,soil conservation and grain production ser-vice in the study area was 10.82%,-0.09%,4.07%and 1.80%,respectively.They all had significant spatial differentiation features;(3)the change of habitat quality,grain production service showed a significant pos-itive correlation with the change of cultivated land area.The change in soil conservation and carbon storage were negatively related to cultivated land area.In order to promote the rational development and utilization of cultivated land in the research area,it is suggested to scientifically carry out the comprehensive utilization of cultivated land reserve resources such as saline-alkali land,strengthen the management and protection of newly added cultivated land at the same time.The results can provide scientific reference for formulating comprehensive land use planning under ecological security conditions.展开更多
Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity (BSP) is the production...Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity (BSP) is the production capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local environment and field management. Based on 22-yr (1990-2011) long-term experimental data on black soil (Typic hapludoll) in Gongzhuling, Jilin Province, Northeast China, the decision support system for an agro-technology transfer (DSSAT)-CERES-Maize model was applied to simulate the yield by BSP of spring maize (Zea mays L.) to examine the effects of long-term fertilization on changes of BSP and explore the mechanisms of BSP increasing. Five treatments were examined: (1) no-fertilization control (control); (2) chemical nitrogen, phosphorus, and potassium (NPK); (3) NPK plus farmyard manure (NPKM); (4) 1.5 time of NPKM (1.5NPKM) and (5) NPK plus straw (NPKS). Results showed that after 22-yr fertilization, the yield by BSP of spring maize significantly increased 78.0, 101.2, and 69.4% under the NPKM, 1.5NPKM and NPKS, respectively, compared to the initial value (in 1992), but not significant under NPK (26.9% increase) and the control (8.9% decrease). The contribution percentage of BSP showed a significant rising trend (P〈0.05) under 1.5NPKM. The average contribution percentage of BSP among fertilizations ranged from 74.4 to 84.7%, and ranked as 1.5NPKM〉NPKM〉NPK〉NPKS, indicating that organic manure combined with chemical fertilizers (I.5NPKM and NPKM) could more effectively increase BSP compared with the inorganic fertilizer application alone (NPK) in the black soil. This study showed that soil organic matter (SOM) was the key factor among various fertility factors that could affect BSP in the black soil, and total N, total P and/or available P also played important role in BSP increasing. Compared with the chemical fertilization, a balanced chemical plus manure or straw fertilization (NPKM or NPKS) not only increased the concentrations of soil nutrient, but also improved the soil physical properties, and structure and diversity of soil microbial population, resulting in an iincrease of BSP. We recommend that a balanced chemical plus manure or straw fertilization (NPKM or NPKS) should be the fertilization practices to enhance spring maize yield and improve BSP in the black soil of Northeast China.展开更多
Characteristics of soil erosion change along a long slope in the gentle hilly areas in black soil region in Northeast China are discussed. A simplified slope model based on segments was used to analyze the runoff data...Characteristics of soil erosion change along a long slope in the gentle hilly areas in black soil region in Northeast China are discussed. A simplified slope model based on segments was used to analyze the runoff data and soil erosion data observed between 2003 and 2004 over 10 field plots with different slope length in Heshan Farm, Heilongjiang Province. We found that soil erosion rate over long slopes in the black soil region changed alternatively along the slope and creates alternative zones of intensive erosion and week erosion.The exact place of each zone is different for different rainfall conditions. In a year with less and mild precipitation, rill cannot happen within the top 50 m, while in a year with large and intensive precipitation, rill can be formed starting even at 15 m from the top of the slope.展开更多
The objective of this paper is to investigate a simple and practical method for soil productivity assessment in the black soil region of Northeast China. Firstly, eight kinds of physicochemical properties for each of ...The objective of this paper is to investigate a simple and practical method for soil productivity assessment in the black soil region of Northeast China. Firstly, eight kinds of physicochemical properties for each of 120 soil samples collected from 25 black soil profiles were analyzed using cluster and correlation analysis. Subsequently, parameter indices were calculated using physicochemical properties. Finally, a modified productivity index (MPI) model were developed and validated. The results showed that the suitable parameters for soil productivity assessment in black soil region of Northeast China were soil available water, soil pH, clay content, and organic matter content. Compared with original productivity index (PI) model, MPI model added clay content and organic matter content in parameters while omitted bulk density. Simulation results of original PI model and MPI model were compared using crop yield of land block where investigated soil profiles were located. MPI model was proven to perform better with a higher significant correlation with maize yield. The correlation equation between MPI and yield was: Y= 3.2002Ln(MP/)+ 10.056, R^2 = 0.7564. The results showed that MPI model was an effective and practical method to assess soil productivity in the research area.展开更多
Water erosion is the major reason for the loss of soil organic carbon in the Northeast China, which leads to the soil quality deterioration and adjacent water pollution. In this study, the effect of extraction tempera...Water erosion is the major reason for the loss of soil organic carbon in the Northeast China, which leads to the soil quality deterioration and adjacent water pollution. In this study, the effect of extraction temperature, pH value, and salt on the water extractable organic matter (WEOM) was determined by means of the UV absorbance, fluorescence excitation-emission matrix, and derived fluorescence indexes. In general, the carbon content and aromaticity of WEOM increased with the increasing of extraction temperature, with the exception that there was no significant difference in the amount at 0 and 20℃. More fluorophores, especially microbially-derived organic matter were extracted at high temperature. The pH values of extractant, including 5, 7, and 10, showed no effect on the carbon amount of WEOM, whereas the aromaticity and microbially-derived component gradually increased with the increasing of pH values. The fluorescence intensity of humic acid-like fluorophore was stronger in neutral and alkali condition than that in acidic condition. The addition of 10 mmol L-1 CaCl2 significantly decreased the carbon amount of recovered WEOM. Moreover, it significantly decreased the aromaticity of WEOM and the quantity of fulvic acid-like and humic acid-like fluorophores, whereas increased the percentage of tyrosine-like and tryptophan-like fluorophores in the total fluorophores and the amount of microbially-derived organic matter. Generally, 10 mmol L-1 KCl showed the same influence trend, but with low influence degree.展开更多
The black soil region of northeast China is one of the most important grain-producing areas in China. Increasingly severe gully erosion in this region has destroyed much farmland and reduced grain production. We analy...The black soil region of northeast China is one of the most important grain-producing areas in China. Increasingly severe gully erosion in this region has destroyed much farmland and reduced grain production. We analyzed SPOT5 imagery from 2007 and TM imagery from 2008 to describe the distributions of gullies and farmland shelterbelts in Kedong County and to assess the effect of farmland shelterbelts on gully erosion. The ima- gery revealed 2311 gullies with average density of 418.51 m km-2, indicating very serious gully erosion. With increasing slope gradient there was an inverse trend between gully density and shelterbelt density, indicating that farmland shelterbelts can prevent gully erosion. The defense effect of farmland shelterbelts against gullyerosion varied with distance: for distances 〈120 m, the defense effect was consistent and very strong; for distances of 120-240 m, a weak linear decrease was found in the defense effect; and for distances 〉240 m, the defense effect of the shelterbelts was significantly weaker. We recommend an optimal planting density of farmland shel- terbelts for the prevention of gully erosion at 1100-1300 m km-2.展开更多
How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical mode...How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical model with N,P,K,irrigation water( W) and water use efficiency( WUE),which was set up under the condition of controlled irrigation with quadratic D- 416 optimized saturation design. The results show that the decending order of single factor' s influence on the WUE was N,K,P and W. All the interactions between N&P,N&K,N&W,K&P,P&W and K&W on the WUE were raised initially,and when reached a certain value,they began to decline. The decending order of each interaction on the WUE was K&P,K&W,N&K,N&P,P&W and N&W. When the WUE was targeted within 1. 8- 2. 5 kg / km^3,an optimized proportion plan was obtained in the 95% confidence interval,i. e. N 87. 76- 103. 32 kg / hm^2,K_2 O 52. 37- 66. 53 kg / hm^2 and P_2O_536. 80- 46. 71 kg / hm^2. Furthermore,the late tillering of the soil moisture content was 70. 07%- 72. 57% of the saturated moisture content.展开更多
By two years (2007-2008) located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, ...By two years (2007-2008) located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers' increasing production range was higher than the single chemical fertilizers' which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.展开更多
In order to get a good indicator to evaluate the impacts of no tillage (NT) on soil structure and soil quality, we studied the dynamics of total soil organic carbon (SOC) and aggregate-associated SOC, and their re...In order to get a good indicator to evaluate the impacts of no tillage (NT) on soil structure and soil quality, we studied the dynamics of total soil organic carbon (SOC) and aggregate-associated SOC, and their relationships in the plow layer (30 cm) in black soil of Northeast China under NT practice. The tillage experiment was established in Dehui County, Jilin Province, China, in 2001. The total SOC and aggregate-associated SOC under 5-yr tillage treatments were measured. NT practices did not lead to the increase of average SOC content at 0-30 cm depth, but it did significantly increase SOC at the top soil (0-5 cm). In NT plots, the change of SOC in 〉 1 000 μm aggregate was the same with that of total SOC, but the effect of NT on SOC in 〉 1 000 lain aggregate was greater than the effect on total SOC, suggesting that 〉 1 000 μm aggregate had more sensitive response to the impact of tillage practices. Also, significant positive correlation occurred between total SOC and SOC in 〉 1 000 μm aggregate in black soil. Consequently, in the short term soil macroaggregate 〉 1 000 μm could be used as an indicator to evaluate the impacts of tillage practices on soil structure in black soil of Northeast China.展开更多
Soil macro-arthropods in the black soil region in Jilin Province of China were investigated with the emphasis laid on the species richness and abundance in relation to the types of land-use, i.e., farm yard, farm land...Soil macro-arthropods in the black soil region in Jilin Province of China were investigated with the emphasis laid on the species richness and abundance in relation to the types of land-use, i.e., farm yard, farm land and Three-North Forest Shelter Belt. Soil macro-arthropods were hand-sorted in the field. A total of 2 357 soil macro-arthropod individuals was captured and fell into 70 families. The results suggested that type of land use affected the species richness and abundance of soil macro-arthropods. Agricultural practices had a strong impact on the soil macro-arthropods community, the conventional cultivations changed the vertical structure of macro-arthropods in the soil profile, and improved the richness and abundance of macro-arthropods in the lower soil layers especially in July. The results also showed that different groups of soil macro-arthropods had various responses to land use changes.展开更多
A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a bl...A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a black soil was conducted from 2004 to 2005 in Heilongjiang Province. The results showed that the nitrogen application rates at 45 kg·hm^-2 with the ratio of 75% NO3-N to 25% NH4-N resulted in the highest potassium and reducing sugar contents in the flue-cured tobacco leaving with the highest quality grade and value. It is recommended that this ni- trogen application rate and NO3-N to NH4-N ratio should be widely applied on flue-cured tobacco grown in the black soil in Heilongjiang Province.展开更多
Regressive models were obtained by employing '311 B' and '3414' optimal regressive desingns through 5 year (1993~1997) field experiment,and by datum processing with computer.These models express the...Regressive models were obtained by employing '311 B' and '3414' optimal regressive desingns through 5 year (1993~1997) field experiment,and by datum processing with computer.These models express the relationships between corn yields in high,middle and low yield areas and N,P,and K application rates in black soil.By analysis to the models,the fertilizer application rates for maximum yield and optimal yield were achieved.展开更多
A total of 1400 soil samples from the plow layer (0-20 cm) at an approximate interval of 5 km were collected in the autumn of 2002 over the entire black soil arable crops region to determine the spatial variability of...A total of 1400 soil samples from the plow layer (0-20 cm) at an approximate interval of 5 km were collected in the autumn of 2002 over the entire black soil arable crops region to determine the spatial variability of seven variables, such as total organic matter content (OMC), total N, total P, total K, alkali-dissolvable N (AN), available P (AP) and available K (AK), with classical statistics and geostatistical analysis across the entire black soil area in Northeast China. In nonsampled areas ordinary kriging was utilized for interpolation of estimated nutrient determinations. Classical statistics revealed highly significant (P≤0.01) correlations with all seven of the soil properties, except for OMC with AP and total K with AK. In addition, using coefficients of variation, all soil properties, except for total K, were moderately variable. A geostatistical analysis indicated that structural factors, such as parent material, terrain, and water table, were the main causes of the spatial correlations. Strong spatial correlations were noted with OMC, total N, total P, AN, and AP, while they were moderate for total K and AK. The effective spatial autocorrelation of OMC, total N, total P, and AN ranged from 1 037 to 1 353 km, whereas the ranges of total K, AP, and AK were only from 6 to 138 km. The fit of the experimental semi-variograms to the theoretical models indicated that except for AN, kriging could successfully interpolate other six variables. Thus, the geostatistical method used on a large scale could accurately evaluate the spatial variability of most black soil nutrient properties in Northeast China.展开更多
Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fer...Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.展开更多
基金Under the auspices of National Natural Science Foundation of China(No.42301296)Postdoctoral Research Foundation of China(No.2022M723130)Key Projects of Social Science Planning Fund of Liaoning Province,China(No.L23AGL001)。
文摘It is of great significance to systematically analyze the cultivated land system resilience(CLSR) for the black soil protection and national food security.The CLSR is impacted by planting structure adjustment and cultivated land quality decline,posing major hidden dangers to food security.It is urgent to evaluate the CLSR at multiple spatio-temporal scales.This study took Liaoning Province in the black soil region of Northeast China as an example.Based on the resilience theory,this study constructed the CLSR evaluation system from the input-feedback perspective at the provincial-scale and the city-scale,and used the rank-sum ratio comprehensive evaluation method(RSR) to analyze the key influencing factors of CLSR in Liaoning Province and its 14 cities from 2000 to 2019.The results showed that:1) the time series changes of CLSR at the provincial-scale and the city-scale in Liaoning Province were similar,both showing an increasing trend.2) The CLSR in Liaoning Province presented a spatial pattern of ‘high in the west and low in the east’ at the city-scale.3) There were seven and six main influencing factors of CLSR at the provincial-scale and the city-scale,respectively.In addition to the net income per capita of rural households,other influencing factors of CLSR were different at the provincial-scale and the city-scale.The feedback factors were dominant at the provincial-scale,and the input factors and feedback factors were dominant at the city-scale.The results could provide a reference for the utilization of black soil and draw on the experience of regional agricultural planning and adjustment.
基金Supported by National Key Research and Development Program of China(2017FYD0101406)Zhoukou Comprehensive Test Station of Henan Provincial Corn Industry Technology System(HARS-22-02-Z5)。
文摘[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.
基金Under the auspices of China Postdoctoral Science Foundation(No.2022M713130)National Natural Science Foundation of China(No.42101212)Strategic Pilot Science and Technology Project of the Chinese Academy of Sciences(No.XDA28020403)。
文摘As the population continues to shrink in the black soil region of Northeast China since 2000,it is critical to master the impact of population shrinkage on rural functions to realize rural revitalization and sustainable development.In this study,we focused on the impacts of depopulation on the evolution and interrelationship of rural subfunctions.Based on the rural function indexes system,the TOPSIS(Technique for Order Preference by Similarity to an Ideal Solution)method,spatial analysis method,and mathematical statistics analysis method were used to summarize the spatial and temporal characteristics of rural function development,as well as the effect of population shrinkage in the typical black soil region of Northeast China.The results showed that depopulation varied in the extent and duration between the forested region and plain areas,which both impacted the trajectories of rural subfunctions evolution.For the economic development function and ecological conservation function,the effect of continuous slight depopulation was beneficial,while the effect of rapid depopulation was adverse,which was exactly opposite to the agricultural production function.All forms of population shrinkage were conducive to the development of the social security function.With the deepening population shrinkage,depopulation mainly promoted the collaborative development between subfunctions in this study,except the relationship between agricultural production and social security function.But effects of depopulation on the interrelationship of rural subfunctions varied between the forested region and plain areas in some cases.The results provided evidence for the cognition that population shrinkage had complicated effects on rural subfunctions.
基金Under the auspices of National Key R&D Program of China(No.2021YFD1500104-4)National Natural Science Foundation of China(No.42171407,42077242)+1 种基金Natural Science Foundation of Jilin Province(No.20210101098JC)Special Investigation on Basic Science and Technology Resources(No.2021FY100406)。
文摘Cultivated land is an important natural resource to ensure food,ecological and economic security.The cultivated land quality evaluation(CQE)is greatly significant for protecting and managing cultivated land.In this study,320 counties in the black soil region of Northeast China(BSRNC)represent the research units used to construct the CQE system measuring the soil properties(SP),cultivated land productivity(CLP),ecological environment(EE)and social economy(SE).The total of 19 factors were selected to calculate the integrated fertility index(IFI)and divided into grades.Simultaneously,we used the coupling coordination degree model to comprehensively analyze the spatial pattern of the cultivated land quality(CLQ)in the BSRNC,and use the structural equation model(SEM)to analyze the driving mechanism.The results show the following:1)The CLQ of 262 counties in the BSRNC is in a state of coupling and coordination,and the coupling and coordination degree presents a spatial distribution pattern of‘high in the southwest and northeast,low in the northwest and southeast’.The coordinated development degree of 271 counties is between 0.4 and 0.6,which is in a transitional state between coordination and disorder.2)The CLQ in the BSRNC is generally good,with an average grade of 3.High-quality cultivated land accounts for 58.45%of all counties,middle-and upper-quality cultivated land accounts for 27.05%,and poor-quality cultivated land accounts for 14.49%.3)The SEM analysis shows that the SP,CLP,EE,and SE all influence the CLQ.Among them,the SP has the largest driving force on the CLQ,while the SE has the smallest driving force on it.The results confirm that the main factors affecting the evaluation results are crop productivity level,normalized difference vegetation index,ratio vegetation index,difference vegetation index,and organic carbon content.When implementing protection measures in counties with a low CLQ,considering a balanced coordination of multiple systems and reasonably controlling the quality degradation are important.This study provides the current situation and driving factors of the CLQ in the BSRNC and will play an important role in black soil governance and utilization.
基金supported by the Open Research Fund of Innovation and Open Laboratory of Eco-meteorology in Northeast China,China Meteorological Administration(stqx2019zd02)Heilongjiang Meteorological Science and Technology Research Project(HQGG202004)Heilongjiang Provincial Natural Science Foundation of China(LH2020C105)。
文摘The dry and windy climate and low ground cover in spring in the black soil region of Northeast China make the soil strongly affected by wind erosion,which seriously threatens the food security and ecological security of this region.In this paper,based on the daily observation data of 124 meteorological stations in study area from 1961 to 2020,seasonal and monthly wind erosion climate factor(C)in spring(March to May)were calculated by using the method proposed by the Food and Agriculture Organization of the United Nations(FAO),the wind erosion characterization in spring were systematically analyzed based on C by various statistical analysis methods.The results showed that in the past 60 years,spring wind erosion climate factor(CSp)and monthly C of the whole region and each province(region)all showed highly significant decreasing trend,but they began to show rebounded trend in the middle or late 2000s.CSp of the study area showed a significant upward trend since 2008 with an increase of 4.59(10a)^(-1).The main contributors to this upward trend are the changes of C in March and in April.For the four provinces(regions),CSp in Heilongjiang,Jilin,Liaoning and eastern Inner Mongolia all showed rebounded since 2008,2011,2008 and 2009,respectively.The rebounded trend of CSp in eastern Inner Mongolia was the most obvious with a tendency rate of 11.27(10a)^(-1),and its mutation occurred after 1984.The rebound trend of CSp in Heilongjiang Province takes the second place,with a trend rate of 4.72(10a)^(-1),but there’s no obvious time mutation characteristics.The spatial characteristics of CSpand monthly C are similar,showing decreasing characteristics centered on the typical black soil belt of Northeast China.Compared with 1961-1990,in the period from 1991 to 2020,the proportion of high value areas(CSp>35,monthly C>10)has decreased to varying degrees,while the proportion of low value areas(CSp≤10,monthly C≤4)has increased.The trends of seasonal and monthly C in 82.2%~87.7%of the stations show significant decreases at 95%confidence level.CSp is closely related to wind speed at 2m height,temperature difference,minimum temperature and precipitation in the same period,of which the correlation between CSp and wind speed is the strongest,indicating that the main control factor for CSp in the study area is wind speed,but the impact of the change of temperature and precipitation on CSp cannot be ignored.
基金Supported by projects of National Natural Science Foundation of China(No.42071255)Jilin Province Philosophy and Social Science Think Tank Fund(No.2023JLSKZKZB066)Special Research of Northeast Revitaliza tion and Development of Jilin University(No.23dbzx10).
文摘It is of great significance to study the influence of spatial pattern change of cultivated land on eco-system service,including the sustainable utilization and ecological protection in black soil region in Northeast China.Taking Lishu County of Jilin Province as the study area,we quantitatively evaluated soil conservation,habitat quality,carbon storage and grain production service,and analyzed the change of cultivated land and ecosystem service pattern in Lishu County from 1990 to 2020 by using Pearson correlation analysis on the correlation between cultivated land change and ecosystem service.The results show that:(1)The cultivated land area of Lishu County was reduced by 19.67 km2,the characteristics of cultivated land change are signifi-cant;(2)the overall change rate of carbon storage,habitat quality,soil conservation and grain production ser-vice in the study area was 10.82%,-0.09%,4.07%and 1.80%,respectively.They all had significant spatial differentiation features;(3)the change of habitat quality,grain production service showed a significant pos-itive correlation with the change of cultivated land area.The change in soil conservation and carbon storage were negatively related to cultivated land area.In order to promote the rational development and utilization of cultivated land in the research area,it is suggested to scientifically carry out the comprehensive utilization of cultivated land reserve resources such as saline-alkali land,strengthen the management and protection of newly added cultivated land at the same time.The results can provide scientific reference for formulating comprehensive land use planning under ecological security conditions.
基金supported by the National 973 Program of China (2011CB100501)the National 863 Program of China(2013AA102901)+1 种基金the Special Fund for Agro-Scientific Research in the Public Interest, China (201203077)the Science and Technology Project for Grain Production, China (2011BAD16B15)
文摘Increasing basic farmland soil productivity has significance in reducing fertilizer application and maintaining high yield of crops. In this study, we defined that the basic soil productivity (BSP) is the production capacity of a farmland soil with its own physical and chemical properties for a specific crop season under local environment and field management. Based on 22-yr (1990-2011) long-term experimental data on black soil (Typic hapludoll) in Gongzhuling, Jilin Province, Northeast China, the decision support system for an agro-technology transfer (DSSAT)-CERES-Maize model was applied to simulate the yield by BSP of spring maize (Zea mays L.) to examine the effects of long-term fertilization on changes of BSP and explore the mechanisms of BSP increasing. Five treatments were examined: (1) no-fertilization control (control); (2) chemical nitrogen, phosphorus, and potassium (NPK); (3) NPK plus farmyard manure (NPKM); (4) 1.5 time of NPKM (1.5NPKM) and (5) NPK plus straw (NPKS). Results showed that after 22-yr fertilization, the yield by BSP of spring maize significantly increased 78.0, 101.2, and 69.4% under the NPKM, 1.5NPKM and NPKS, respectively, compared to the initial value (in 1992), but not significant under NPK (26.9% increase) and the control (8.9% decrease). The contribution percentage of BSP showed a significant rising trend (P〈0.05) under 1.5NPKM. The average contribution percentage of BSP among fertilizations ranged from 74.4 to 84.7%, and ranked as 1.5NPKM〉NPKM〉NPK〉NPKS, indicating that organic manure combined with chemical fertilizers (I.5NPKM and NPKM) could more effectively increase BSP compared with the inorganic fertilizer application alone (NPK) in the black soil. This study showed that soil organic matter (SOM) was the key factor among various fertility factors that could affect BSP in the black soil, and total N, total P and/or available P also played important role in BSP increasing. Compared with the chemical fertilization, a balanced chemical plus manure or straw fertilization (NPKM or NPKS) not only increased the concentrations of soil nutrient, but also improved the soil physical properties, and structure and diversity of soil microbial population, resulting in an iincrease of BSP. We recommend that a balanced chemical plus manure or straw fertilization (NPKM or NPKS) should be the fertilization practices to enhance spring maize yield and improve BSP in the black soil of Northeast China.
基金National Basic Research Program of China, No.2007CB407207Knowledge Innovation Project of Institute of Geographic Sciences and Natural Resources Research,CAS,No.CXIOG-A04-10the support from CAS through its "One Hundred Talent" program
文摘Characteristics of soil erosion change along a long slope in the gentle hilly areas in black soil region in Northeast China are discussed. A simplified slope model based on segments was used to analyze the runoff data and soil erosion data observed between 2003 and 2004 over 10 field plots with different slope length in Heshan Farm, Heilongjiang Province. We found that soil erosion rate over long slopes in the black soil region changed alternatively along the slope and creates alternative zones of intensive erosion and week erosion.The exact place of each zone is different for different rainfall conditions. In a year with less and mild precipitation, rill cannot happen within the top 50 m, while in a year with large and intensive precipitation, rill can be formed starting even at 15 m from the top of the slope.
基金supported by the National Natural Science Foundation of China (40671111)
文摘The objective of this paper is to investigate a simple and practical method for soil productivity assessment in the black soil region of Northeast China. Firstly, eight kinds of physicochemical properties for each of 120 soil samples collected from 25 black soil profiles were analyzed using cluster and correlation analysis. Subsequently, parameter indices were calculated using physicochemical properties. Finally, a modified productivity index (MPI) model were developed and validated. The results showed that the suitable parameters for soil productivity assessment in black soil region of Northeast China were soil available water, soil pH, clay content, and organic matter content. Compared with original productivity index (PI) model, MPI model added clay content and organic matter content in parameters while omitted bulk density. Simulation results of original PI model and MPI model were compared using crop yield of land block where investigated soil profiles were located. MPI model was proven to perform better with a higher significant correlation with maize yield. The correlation equation between MPI and yield was: Y= 3.2002Ln(MP/)+ 10.056, R^2 = 0.7564. The results showed that MPI model was an effective and practical method to assess soil productivity in the research area.
基金supported by the National Natural Science Foundation of China (51109089 and 31071862)
文摘Water erosion is the major reason for the loss of soil organic carbon in the Northeast China, which leads to the soil quality deterioration and adjacent water pollution. In this study, the effect of extraction temperature, pH value, and salt on the water extractable organic matter (WEOM) was determined by means of the UV absorbance, fluorescence excitation-emission matrix, and derived fluorescence indexes. In general, the carbon content and aromaticity of WEOM increased with the increasing of extraction temperature, with the exception that there was no significant difference in the amount at 0 and 20℃. More fluorophores, especially microbially-derived organic matter were extracted at high temperature. The pH values of extractant, including 5, 7, and 10, showed no effect on the carbon amount of WEOM, whereas the aromaticity and microbially-derived component gradually increased with the increasing of pH values. The fluorescence intensity of humic acid-like fluorophore was stronger in neutral and alkali condition than that in acidic condition. The addition of 10 mmol L-1 CaCl2 significantly decreased the carbon amount of recovered WEOM. Moreover, it significantly decreased the aromaticity of WEOM and the quantity of fulvic acid-like and humic acid-like fluorophores, whereas increased the percentage of tyrosine-like and tryptophan-like fluorophores in the total fluorophores and the amount of microbially-derived organic matter. Generally, 10 mmol L-1 KCl showed the same influence trend, but with low influence degree.
基金supported by the National Natural Science Foundation of China(31400612,41271305)the Key Technologies Research and Development Program of Henan Province(142102110147)
文摘The black soil region of northeast China is one of the most important grain-producing areas in China. Increasingly severe gully erosion in this region has destroyed much farmland and reduced grain production. We analyzed SPOT5 imagery from 2007 and TM imagery from 2008 to describe the distributions of gullies and farmland shelterbelts in Kedong County and to assess the effect of farmland shelterbelts on gully erosion. The ima- gery revealed 2311 gullies with average density of 418.51 m km-2, indicating very serious gully erosion. With increasing slope gradient there was an inverse trend between gully density and shelterbelt density, indicating that farmland shelterbelts can prevent gully erosion. The defense effect of farmland shelterbelts against gullyerosion varied with distance: for distances 〈120 m, the defense effect was consistent and very strong; for distances of 120-240 m, a weak linear decrease was found in the defense effect; and for distances 〉240 m, the defense effect of the shelterbelts was significantly weaker. We recommend an optimal planting density of farmland shel- terbelts for the prevention of gully erosion at 1100-1300 m km-2.
文摘How to improve the water use efficiency of rice in black soil regions was studied. The black soil region in paddy fields was chosen as the research object. The research showed the fertilizer coupling mathematical model with N,P,K,irrigation water( W) and water use efficiency( WUE),which was set up under the condition of controlled irrigation with quadratic D- 416 optimized saturation design. The results show that the decending order of single factor' s influence on the WUE was N,K,P and W. All the interactions between N&P,N&K,N&W,K&P,P&W and K&W on the WUE were raised initially,and when reached a certain value,they began to decline. The decending order of each interaction on the WUE was K&P,K&W,N&K,N&P,P&W and N&W. When the WUE was targeted within 1. 8- 2. 5 kg / km^3,an optimized proportion plan was obtained in the 95% confidence interval,i. e. N 87. 76- 103. 32 kg / hm^2,K_2 O 52. 37- 66. 53 kg / hm^2 and P_2O_536. 80- 46. 71 kg / hm^2. Furthermore,the late tillering of the soil moisture content was 70. 07%- 72. 57% of the saturated moisture content.
基金Supported by Black Soil Conservation Tillage and Oriented Cultivation Technology Research (GB06B107-1)Innovation Fund of Northeast Agricultural University (CXP7003-3-3)+1 种基金Northeast Agricultural University and the Scientific Research Fund of Heilongjiang Province to Black CollegesUniversities Cold Key Laboratory of Utilization and Protection of Open-funded Projects (GXS08-5)
文摘By two years (2007-2008) located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers' increasing production range was higher than the single chemical fertilizers' which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.
基金supported by the National Natural Science Foundation of China (40801071)the Key Technologies R&D Program of China during the 11th Five-Year Plan period (2006BAD15B01)+1 种基金the Program for Advanced Science Field (KZCX3-SW-NA3-31)Doctoral Research Foundation in Northeast Institute of Geography and Agroecology,Chinese Academy of Sciences (O8H2041)
文摘In order to get a good indicator to evaluate the impacts of no tillage (NT) on soil structure and soil quality, we studied the dynamics of total soil organic carbon (SOC) and aggregate-associated SOC, and their relationships in the plow layer (30 cm) in black soil of Northeast China under NT practice. The tillage experiment was established in Dehui County, Jilin Province, China, in 2001. The total SOC and aggregate-associated SOC under 5-yr tillage treatments were measured. NT practices did not lead to the increase of average SOC content at 0-30 cm depth, but it did significantly increase SOC at the top soil (0-5 cm). In NT plots, the change of SOC in 〉 1 000 μm aggregate was the same with that of total SOC, but the effect of NT on SOC in 〉 1 000 lain aggregate was greater than the effect on total SOC, suggesting that 〉 1 000 μm aggregate had more sensitive response to the impact of tillage practices. Also, significant positive correlation occurred between total SOC and SOC in 〉 1 000 μm aggregate in black soil. Consequently, in the short term soil macroaggregate 〉 1 000 μm could be used as an indicator to evaluate the impacts of tillage practices on soil structure in black soil of Northeast China.
文摘Soil macro-arthropods in the black soil region in Jilin Province of China were investigated with the emphasis laid on the species richness and abundance in relation to the types of land-use, i.e., farm yard, farm land and Three-North Forest Shelter Belt. Soil macro-arthropods were hand-sorted in the field. A total of 2 357 soil macro-arthropod individuals was captured and fell into 70 families. The results suggested that type of land use affected the species richness and abundance of soil macro-arthropods. Agricultural practices had a strong impact on the soil macro-arthropods community, the conventional cultivations changed the vertical structure of macro-arthropods in the soil profile, and improved the richness and abundance of macro-arthropods in the lower soil layers especially in July. The results also showed that different groups of soil macro-arthropods had various responses to land use changes.
文摘A field experiment about effects of nitrogen application rates and different NO3-N to NH4-N ratios on agronomic, chemical and biological characteristics as well as yield and quality of flue-cured tobacco grown in a black soil was conducted from 2004 to 2005 in Heilongjiang Province. The results showed that the nitrogen application rates at 45 kg·hm^-2 with the ratio of 75% NO3-N to 25% NH4-N resulted in the highest potassium and reducing sugar contents in the flue-cured tobacco leaving with the highest quality grade and value. It is recommended that this ni- trogen application rate and NO3-N to NH4-N ratio should be widely applied on flue-cured tobacco grown in the black soil in Heilongjiang Province.
文摘Regressive models were obtained by employing '311 B' and '3414' optimal regressive desingns through 5 year (1993~1997) field experiment,and by datum processing with computer.These models express the relationships between corn yields in high,middle and low yield areas and N,P,and K application rates in black soil.By analysis to the models,the fertilizer application rates for maximum yield and optimal yield were achieved.
基金Project supported by the National Basic Research Program (973 Program) of China (No. 2005CB121108) the Heilongjiang Provincial Natural Science Foundation of China (No. C2004-25).
文摘A total of 1400 soil samples from the plow layer (0-20 cm) at an approximate interval of 5 km were collected in the autumn of 2002 over the entire black soil arable crops region to determine the spatial variability of seven variables, such as total organic matter content (OMC), total N, total P, total K, alkali-dissolvable N (AN), available P (AP) and available K (AK), with classical statistics and geostatistical analysis across the entire black soil area in Northeast China. In nonsampled areas ordinary kriging was utilized for interpolation of estimated nutrient determinations. Classical statistics revealed highly significant (P≤0.01) correlations with all seven of the soil properties, except for OMC with AP and total K with AK. In addition, using coefficients of variation, all soil properties, except for total K, were moderately variable. A geostatistical analysis indicated that structural factors, such as parent material, terrain, and water table, were the main causes of the spatial correlations. Strong spatial correlations were noted with OMC, total N, total P, AN, and AP, while they were moderate for total K and AK. The effective spatial autocorrelation of OMC, total N, total P, and AN ranged from 1 037 to 1 353 km, whereas the ranges of total K, AP, and AK were only from 6 to 138 km. The fit of the experimental semi-variograms to the theoretical models indicated that except for AN, kriging could successfully interpolate other six variables. Thus, the geostatistical method used on a large scale could accurately evaluate the spatial variability of most black soil nutrient properties in Northeast China.
基金the Heilongjiang Provincial Natural Science Funds for Distinguished Young Scholars, China (No.JC200622)the Heilongjiang Provincial Natural Science Foundation of China (No.C2004-17)the National "Eleventh Five Years Plan" Key Project on Science and Technology of China (No.2006BAD25B05).
文摘Black soil(Mollisol) is one of the main soil types in northeastern China. Biolog and polymerase chain reactiondenaturing gradient gel electrophoresis(PCR-DGGE) methods were used to examine the infiuence of various fertilizer combinations on the structure and function of the bacterial community in a black soil collected from Harbin,Heilongjiang Province. Biolog results showed that substrate richness and catabolic diversity of the soil bacterial community were the greatest in the chemical fertilizer and chemical fertilizer+manure treatments. The metabolic ability of the bacterial community in the manure treatment was similar to the control. DGGE fingerprinting indicated similarity in the distribution of most 16S rDNA bands among all treatments,suggesting that microorganisms with those bands were stable and not infiuenced by fertilization. However,chemical fertilizer increased the diversity of soil bacterial community. Principal component analysis of Biolog and DGGE data revealed that the structure and function of the bacterial community were similar in the control and manure treatments,suggesting that the application of manure increased the soil microbial population,but had no effect on the bacterial community structure. Catabolic function was similar in the chemical fertilizer and chemical fertilizer+manure treatments,but the composition structure of the soil microbes differed between them. The use of chemical fertilizers could result in a decline in the catabolic activity of fast-growing or eutrophic bacteria.