It has been suggested that progression of bladder transitional cell cancer （BTCC） may be regulated at the molecular level by a typical pattern of expression of genes involved in apoptosis. Recently Livin, belonging ...It has been suggested that progression of bladder transitional cell cancer （BTCC） may be regulated at the molecular level by a typical pattern of expression of genes involved in apoptosis. Recently Livin, belonging to the inhibitors of apoptosis （IAP） family, has been found to be expressed in most solid tumors, where its expression is suggested to have clinical significance. In order to explore the significance of Livin expression in the development of BTCC, immunohistochemistry and RT-QPCR were used to detect the expression of Livin mRNA in tumor tissues and adjacent normal tissues of 30 cases of BTCC. The results showed that the positive rate of Livin expression in adjacent normal tissues and tumor tissues was 0 and 60% （18/30） respectively. The -△△CT value of Livin in BTCC tissues was 8.0454 （7.4264-8.6644） times of that in adjacent normal tissues. The expression of Livin mRNA had no correlation with tumor pathological grades and clinical stages. It was suggested that there was weak expression of Livin mRNA in adjacent normal tissues, but strong in tumor tissues.展开更多
目的:研究RhoGTP酶GDP解离抑制因子2(rho GDP dissociation inhibitor 2,RhoGDI2)蛋白在膀胱癌组织中的表达情况及意义。方法:收集来自广西医科大学附属肿瘤医院2010年10月至2014年6月的膀胱癌组织标本44例。用免疫组织化学的方法定位...目的:研究RhoGTP酶GDP解离抑制因子2(rho GDP dissociation inhibitor 2,RhoGDI2)蛋白在膀胱癌组织中的表达情况及意义。方法:收集来自广西医科大学附属肿瘤医院2010年10月至2014年6月的膀胱癌组织标本44例。用免疫组织化学的方法定位、定量检测膀胱癌组织中RhoGDI2蛋白的表达情况,按不同病理参数对膀胱癌组织进行分组并比较各分组RhoGDI2蛋白的表达是否有统计学差异。结果:全部膀胱癌组织标本均可见RhoGDI2蛋白阳性表达,不同性别和不同年龄分组的RhoGDI2蛋白表达无差异(P>0.05)。在Ta^T3期,RhoGDI2蛋白表达与分期相关(P<0.05),随着分期增高表达降低,T3与T4期比较无差异(P>0.05);RhoGDI2蛋白表达与分化相关(P<0.05),分化程度越高,表达越低;原发与复发、非转移与转移组之间比较有差异(P<0.05),复发组和转移组的RhoGDI2蛋白表达降低。结论:膀胱癌组织中RhoGDI2蛋白可能抑制膀胱癌的浸润、转移,在蛋白水平上可能是膀胱癌转移抑制因子。展开更多
文摘It has been suggested that progression of bladder transitional cell cancer （BTCC） may be regulated at the molecular level by a typical pattern of expression of genes involved in apoptosis. Recently Livin, belonging to the inhibitors of apoptosis （IAP） family, has been found to be expressed in most solid tumors, where its expression is suggested to have clinical significance. In order to explore the significance of Livin expression in the development of BTCC, immunohistochemistry and RT-QPCR were used to detect the expression of Livin mRNA in tumor tissues and adjacent normal tissues of 30 cases of BTCC. The results showed that the positive rate of Livin expression in adjacent normal tissues and tumor tissues was 0 and 60% （18/30） respectively. The -△△CT value of Livin in BTCC tissues was 8.0454 （7.4264-8.6644） times of that in adjacent normal tissues. The expression of Livin mRNA had no correlation with tumor pathological grades and clinical stages. It was suggested that there was weak expression of Livin mRNA in adjacent normal tissues, but strong in tumor tissues.
文摘目的:研究RhoGTP酶GDP解离抑制因子2(rho GDP dissociation inhibitor 2,RhoGDI2)蛋白在膀胱癌组织中的表达情况及意义。方法:收集来自广西医科大学附属肿瘤医院2010年10月至2014年6月的膀胱癌组织标本44例。用免疫组织化学的方法定位、定量检测膀胱癌组织中RhoGDI2蛋白的表达情况,按不同病理参数对膀胱癌组织进行分组并比较各分组RhoGDI2蛋白的表达是否有统计学差异。结果:全部膀胱癌组织标本均可见RhoGDI2蛋白阳性表达,不同性别和不同年龄分组的RhoGDI2蛋白表达无差异(P>0.05)。在Ta^T3期,RhoGDI2蛋白表达与分期相关(P<0.05),随着分期增高表达降低,T3与T4期比较无差异(P>0.05);RhoGDI2蛋白表达与分化相关(P<0.05),分化程度越高,表达越低;原发与复发、非转移与转移组之间比较有差异(P<0.05),复发组和转移组的RhoGDI2蛋白表达降低。结论:膀胱癌组织中RhoGDI2蛋白可能抑制膀胱癌的浸润、转移,在蛋白水平上可能是膀胱癌转移抑制因子。
