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巴西蘑菇Agaricus blazei Murill水溶性多糖的分离纯化 被引量:3
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作者 林跃鑫 叶竹秋 +1 位作者 黄谚谚 谢华玲 《药物生物技术》 CAS CSCD 2003年第4期238-241,共4页
从巴西蘑菇子实体中提取的水溶性粗多糖经过脱蛋白、脱色等步骤获得半纯品多糖。依次采用DE AE 纤维素和SepharoseCL 4B柱层析进一步纯化后 ,获得 5种水溶性多糖组分。其中主要组分AB I、AB II b和AB III b得率分别为 3.3%、1.5 2 %、0 ... 从巴西蘑菇子实体中提取的水溶性粗多糖经过脱蛋白、脱色等步骤获得半纯品多糖。依次采用DE AE 纤维素和SepharoseCL 4B柱层析进一步纯化后 ,获得 5种水溶性多糖组分。其中主要组分AB I、AB II b和AB III b得率分别为 3.3%、1.5 2 %、0 .85 %。SephadexG 10 0凝胶层析、旋光度和HPLC鉴定各组分已达到层析纯。 展开更多
关键词 巴西蘑菇 多糖 提取 纯化 Agaricus-blazei-Murill水溶性多糖
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Agaricus Blazei Murrill菌种分离与培养研究 被引量:2
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作者 杨旭 《哈尔滨师范大学自然科学学报》 CAS 1999年第6期92-94,共3页
本文报导了 Agaricus blazei murrill子实体形态 ,母种、原种和栽培种的分离与培养 .
关键词 子实体 分离 培养 真菌 AgaricusblazeiMurrill
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姬松茸(Agaricus blazei Murill)生理活性物质的研究进展 被引量:28
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作者 张卉 刘长江 《沈阳农业大学学报》 CAS CSCD 2003年第1期59-62,共4页
回顾了姬松茸的研究历史 ,并对姬松茸多糖、凝集素、甾醇类物质、核酸。
关键词 姬松茸 生理活性物质 多糖 凝集素 甾醇类物质 核酸 脂质 维生素 食药兼用真菌
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巴西蘑菇Agaricus blazei Murill水溶性多糖部分理化性质的研究 被引量:3
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作者 林跃鑫 叶竹秋 +1 位作者 黄谚谚 谢华玲 《食品科学》 EI CAS CSCD 北大核心 2002年第9期31-33,共3页
本文以物理及化学方法研究了巴西蘑菇AgaricusblazeiMurill水溶性多糖AB-I、AB-II-b和AB-III-b的理化性质,结果表明,3种经纯化的多糖都不含糖醛酸而含蛋白质,蛋白质含量分别为AB-I:20.1%、AB-II-b:16.3%、AB-III-b:25.6%。它们的比旋度... 本文以物理及化学方法研究了巴西蘑菇AgaricusblazeiMurill水溶性多糖AB-I、AB-II-b和AB-III-b的理化性质,结果表明,3种经纯化的多糖都不含糖醛酸而含蛋白质,蛋白质含量分别为AB-I:20.1%、AB-II-b:16.3%、AB-III-b:25.6%。它们的比旋度分别为AB-I:+44.33°、AB-II-b;+3.70°、AB-III-b:+5.04°。AB-I、AB-II-b的1.0%水溶液均具有良好的增稠性,其粘度具有很好的抗盐浓度、耐热和对广泛pH的稳定性能。 展开更多
关键词 水溶性多糖 理化性质 巴西蘑菇 食用菌 药用菌
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姬松茸(Agaricus blazei Murrill.) gpd启动子的克隆及其序列分析 被引量:3
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作者 邓勋 宋小双 宋瑞清 《安徽农业科学》 CAS 北大核心 2009年第3期993-995,998,共4页
[目的]对姬松茸中分离的gpd启动子片段进行序列分析。[方法]根据报道的gpd启动子序列设计合成1对引物,以姬松茸基因组DNA为模板,采用PCR扩增法克隆得到了2条DNA特异性片段,并利用启动子信号扫描和启动子预测程序对2条序列的片段启动子... [目的]对姬松茸中分离的gpd启动子片段进行序列分析。[方法]根据报道的gpd启动子序列设计合成1对引物,以姬松茸基因组DNA为模板,采用PCR扩增法克隆得到了2条DNA特异性片段,并利用启动子信号扫描和启动子预测程序对2条序列的片段启动子区和转录结合位点进行分析。[结果]测序结果表明,2条序列分别为372 bp和614 bp,分别命名为J-gpd1和J-gpd2,GenBank登陆号分别为:EU220746和EU220747;J-gpd1有3个,J-gpd2有4个核心启动子区;2条序列不仅具有基本启动子作用元件CAATBOX和TATABOX外,还具有多个重要作用元件,如CGCGBOX、GATABOX、ASF-motif、W-BOX、TGACGT-motif和GTGA motif等。利用TFSEARCH ver.1.3分析表明,2条序列还含有多个转录因子结合位点,包括GCR1、ADR1、HSF、ABF1和RAP1等。[结论]研究推测,2条序列所启动的基因具有较高的多样性和准确性。 展开更多
关键词 姬松茸 gpd启动子 克隆 序列分析
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用稻草栽培巴氏菇(Agaricusblazei Murrill)技术初步研究 被引量:3
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作者 杨旭 《东北农业大学学报》 CAS CSCD 2000年第1期102-104,共3页
报道了用稻草栽培巴氏菇的技术。通过组织分离法培养种菌 ;用大麦粒制作栽培菌种 ;用稻草、米糠、鸡粪和其它配料做栽培料 ,堆积发酵。堆高 1.50 m,宽 2 m,长度不限 ,每 2 d翻堆 1次 ,共翻 4~ 5次 ;栽培料装入栽培床的厚度为 2 0 cm,... 报道了用稻草栽培巴氏菇的技术。通过组织分离法培养种菌 ;用大麦粒制作栽培菌种 ;用稻草、米糠、鸡粪和其它配料做栽培料 ,堆积发酵。堆高 1.50 m,宽 2 m,长度不限 ,每 2 d翻堆 1次 ,共翻 4~ 5次 ;栽培料装入栽培床的厚度为 2 0 cm,其含水率为 6 0 %~ 6 5% ;料温 2 5℃播种 ,用量 3~ 5瓶 / m2 ;8~ 10 d后覆上消毒的稻田土 3~ 4 cm;4周后出菇 ;栽培料的 p H值 6 .5~7.0 ;出菇相对湿度为 90 %~ 95% ;采菇时洗净菇柄基部的泥土 ,纵切后以 6 0℃烘干 ;产量为 1kg/m2 。栽培料发酵与栽培时保温、保湿与适当通风的条件 。 展开更多
关键词 稻草 巴氏菇 栽培技术 产量 药用菌 食用菌
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Biodegrading Wheat Bran with Agaricus blazei and Its Effects on Intestinal Development Identified with Mice 被引量:1
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作者 SHEN Heng-sheng CHEN Jun-chen +2 位作者 WU Li LI Yi-bin Ting Zhou 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2012年第3期456-464,共9页
Agaricus blazei Murill is a well known edible/medicinal mushroom used for immune-nutrient food therapies.The biodegradation of different substrates with this fungus may result in different metabolites and degraded com... Agaricus blazei Murill is a well known edible/medicinal mushroom used for immune-nutrient food therapies.The biodegradation of different substrates with this fungus may result in different metabolites and degraded compounds,which may enrich the function of the food therapies.In this study,soluble compounds from the culture of A.blazei grown in liquid media with whole wheat bran and its water filtrate,respectively,were compared.Total soluble sugar,arabinoxylan,protein,and amino acids were significantly higher in the fungal culture resulted from the medium with whole wheat bran (43.54,1.56,0.59,and 2.19 mg mL^-1,respectively) than that from the medium with the bran filtrate (17.28,0.37,0.13,and 1.13 mg mL^-1,respectively).The biodegraded wheat bran with cultured mycelia was fed to Mus musculus Linnaeus as supplementation or as dietary fiber exclusive ingredient.As non-specific food therapies,feeding effects on mice intestinal development were indicated indirectly in growth performance,intestinal absorption and serum parameters.Compared to feeding uncultured wheat bran,feeding mice with culture mixes of A.blazei resulted in remarkably increase in villus height and villus height/crypt depth ratio,which were increased by 25.4 and 31.0%,respectively,when applied as supplementation,and by 44.3 and 43.4%,respectively,when applied as dietary fiber.These increases are concomitant with the higher level of D-xylose in blood serum about 16.9 and 29.2% as supplementation and dietary fiber,respectively.The results implying that culturing A.blazei with whole wheat bran enhanced extracellular metabolism of the fungus and extensive degradation of wheat bran insoluble fibrous compounds.Furthermore,feeding the culture mix including metabolites and degraded wheat bran improved intestinal villus development,proving the nutritional benefits of the A.blazei mycelial cultures. 展开更多
关键词 Agaricus blazei mycelium metabolite wheat bran submerged culture dietary fiber MICE intestinal villus
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Study on Anti-infection Effect of Polysaccharide from Agaricus blazei Murrill on Mice 被引量:2
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作者 Chao REN Yuqing ZHOU +5 位作者 Shuifeng XIAN Jie LI Deliang WEI Yao LU Nina JIA Shunzi YUAN 《Agricultural Biotechnology》 CAS 2018年第4期102-105,共4页
In order to study the anti-infection effect of phoshporylated Agaricus blazei polysaccharide on mice, mice were drenched phoshporylated A. blazei polysaccharide for 14 d, and an A. blazei polysaccharide control group ... In order to study the anti-infection effect of phoshporylated Agaricus blazei polysaccharide on mice, mice were drenched phoshporylated A. blazei polysaccharide for 14 d, and an A. blazei polysaccharide control group and a blank control group were also set. The mice in all the three groups were infected with Escherichia coli on the 15 th day and the 29 th day of the experiment, the body weight of the mice in each group was recorded, as well as the spleen index on the 29 th day of infection and the 36 th day of the experiment, and the anti- E. coli titer in serum was also detected. The results showed that phoshporylated A. blazei polysaccharide could promote and maintain the development of spleen, and improve the immune response of organisms, thereby resisting bacterial infection. Furthermore, phoshporylated A. blazei polysaccharide has better immunoregulation and anti-infection effects than A. blazei polysaccharide, and could play an important role in veterinary clinical treatment and prevention and control of diseases as a immunologic adjuvant or immunopotentiator. 展开更多
关键词 Polysaccharide from Agaricus blazei Murrill PHOSPHORYLATION anti-infection effect MOUSE Escherichia coli body weight Spleen index ELISA
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Transcriptome Sequencing and Analysis of Agaricus blazei Mycelia Under Cadmium Stress 被引量:1
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作者 ZHANG Yu-hui MA Xin-wang +2 位作者 XIA Zhi-lan HUANG Min-feng SU Rong-rong 《Agricultural Science & Technology》 CAS 2022年第4期54-64,共11页
To explore the mechanism of Agaricus blazei Murrill enrichment of the heavy metal cadmium,we employed Illumina high-throughput sequencing to analyze the transcriptomes of A.blazei mycelia treated with and without exog... To explore the mechanism of Agaricus blazei Murrill enrichment of the heavy metal cadmium,we employed Illumina high-throughput sequencing to analyze the transcriptomes of A.blazei mycelia treated with and without exogenous cadmium addition,and then the differentially expressed genes(DEGs)of the strains with high and low cadmium enrichment between the control and cadmium treatment were screened out.The results showed that the DEGs were mainly involved in steroid biosynthesis,antibiotic biosynthesis,protein processing in endoplasmic reticulum,glutathione metabolism and other pathways.Carbon metabolism and glutathione metabolism may play an important role in the response of A.blazei mycelium to cadmium stress. 展开更多
关键词 Agaricus blazei Murrill Cadmium stress Transcriptome sequencing GO analysis
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Effect of Hot Water Extract from Agaricus Blazei Murill on Chemotaxis of Neutrophils 被引量:1
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作者 Mayuko Miyagawa Yuriko Hirono +5 位作者 Ayaka Kawazoe Eri Shigeyoshi Masahito Nose Masaaki Sakura K. E. Pinkerton Minoru Takeuchi 《Journal of Cosmetics, Dermatological Sciences and Applications》 2013年第1期12-17,共6页
Hot water extract from the edible Brazilian mushroom, Agaricus Blazei Murill (ABM), is used for both traditional and alternative medicine. ABM is reported to stimulate anti-tumor, anti-infection, and immune activity. ... Hot water extract from the edible Brazilian mushroom, Agaricus Blazei Murill (ABM), is used for both traditional and alternative medicine. ABM is reported to stimulate anti-tumor, anti-infection, and immune activity. However, there are few reports of how ABM affects neutrophils. Therefore, in this study, we examined the effect of hot water ABM extract on neutrophil migration, phagocytosis, and reactive oxygen species production using neutrophils from guinea pig. Migratory direction and velocity as indicators of chemotactic activity of neutrophils were significantly (p 0.001) increased at concentration of 50 and 100 mg/ml in ABM extract compared with control. Phagocytic activity of neutrophil was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. Production of reactive oxygen species (ROS: H2O2 or ) by neutrophils was significantly (p 0.01) increased at concentration of 5 mg/ml in ABM extract compared with control. These results suggest that enhancement in neutrophil chemotactic activity, phagocytic activity and ROS production are mechanisms by which ABM extract inhibits bacterial infection in the skin and dermatitis. 展开更多
关键词 NEUTROPHIL Chemotactic ACTIVITY Phagocytic ACTIVITY ROS Production AGARICUS blazei Murill
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Study on Several Characteristics of Agaricus blazei MurrilStrain J_3 Irradiated by ^(60)Co
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作者 WENG Bo-qi, JIANG Zhi-he, HUANG Ting-jun, CHEN Jian and ZHENG Wei-wen(Fujian Academy of Agricultural Sciences , Fuzhou 350003 , P. R. China) 《Agricultural Sciences in China》 CAS CSCD 2003年第8期843-849,共7页
A new mutant strain J3 from Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations were increased more than 70% to compare with their original strain. The component analysis on a... A new mutant strain J3 from Agaricus blazei Murril was obtained by 60Co irradiation. The yields of successive generations were increased more than 70% to compare with their original strain. The component analysis on amino acids and fatty acid illuminated that the nutrient value of strain J3 fruiting body was better than the original strain. The apparent nodule structure was found in the hyphea of J3 strain through the observation by scanning electron microscope. RAPD analysis showed the great difference of PCR fingerprints between J3 and its original strain. It is a promising mutant strain for further commercial development in the future. 展开更多
关键词 Agaricus blazei Murril 60Co irradiation ULTRASTRUCTURE RAPD analysis Nutrient evaluation
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Research of Submerge Fermentation Conditions on <i>Agaricus blazei</i>Murill
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作者 Xinzhi Cao Yingqing Zhao +2 位作者 Li Xiong Linsheng Ren Jia Liu 《Food and Nutrition Sciences》 2014年第19期1926-1943,共18页
Rotational speed, temperature, inoculation amount and pH are the four factors that affect the submerge fermentation of Agaricus blazei Murill. The orthogonal experiment was on the basis of single factor experiments. A... Rotational speed, temperature, inoculation amount and pH are the four factors that affect the submerge fermentation of Agaricus blazei Murill. The orthogonal experiment was on the basis of single factor experiments. And the optimal submerge fermentation condition of Agaricus blazei Murill was determined through orthogonal test. The results were as follows: temperature (25℃), rotational speed (150 r/min), inoculation amount (6%) and pH (7), in this condition the cell dry weight reached 1.32 g/100mL;temperature (25℃), rotational speed (150 r/min), inoculation amount (8%) and pH (6.5) in this condition the extracellular polysaccharide reached 6.95 mg/mL. 展开更多
关键词 AGARICUS blazei Murill SUBMERGED Fermentation Cell Dry Weight EXTRACELLULAR POLYSACCHARIDE
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Effects of Arterially Infused Hydroalcoholic <i>Agaricus blazei</i>Extracts on Perfusion Pressure and Oxygen Uptake in the Bivascularly Perfused Rat Liver
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作者 Andrea Luiza de Oliveira Valoto Jorgete Constantin +2 位作者 Fabrício Bracht Rosane Marina Peralta Adelar Bracht 《Journal of Biosciences and Medicines》 2015年第10期74-79,共6页
In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vei... In a preceding work we have reported experiments showing that an hydroalcoholic exctract of Agaricus blazei is able to exert purinergic effects in the isolated perfused rat liver when it is infused into the portal vein in monovascular perfusion (entry: portal vein;exit: hepatic vein). In the present communication we are presenting and discussing experiments done with the bivascularly perfused rat liver (entry: portal vein + hepatic artery;exit: hepatic vein) in order to verify if the hemodynamic effects also occur in the arterial bed. It was found that the A. blazei extract is also active when infused into the hepatic arterial bed, with differences in both sensitivity and nature of the effects on either perfusion pressure or oxygen consumption. Constriction of the arterial bed required much higher concentrations of the extract than the portal bed. The kinetics of the response was also different, with a biphasic instead of a monophasic response. These results provide a promising starting point for future studies aiming to bring to light more mechanistic details about these and possibly other effects. 展开更多
关键词 AGARICUS blazei PURINERGIC Action HEMODYNAMICS Oxygen Uptake
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Effects of Agaricus blazei Murill Polysaccharides on Leadinduced Disturbance of Morphology and ASIC1a and ASIC2b Gene Expression in Hippocampus of Rats
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作者 Xin LI Xiru LV +3 位作者 Cuiping FENG Mingchang CHANG Hongyan CHENG Junlong MENG 《Agricultural Biotechnology》 CAS 2014年第3期56-59,共4页
The present study was undertaken to investigate the effects of Agaricus blazei murill polysaccharides on morphology of hippocampus tissue and expression of ASIC1a and ASIC2b mRNA in lead-poisoning rats.Forty SD rats w... The present study was undertaken to investigate the effects of Agaricus blazei murill polysaccharides on morphology of hippocampus tissue and expression of ASIC1a and ASIC2b mRNA in lead-poisoning rats.Forty SD rats were divided into five groups:control group,lead group,experimental group 1,2,3.After 60d of treatments,hippocampus tissues were observed by HE staining and the expression levels of ASIC1a and ASIC2b mRNA were determined by the flouoreescent quantitative real-time polymerase chain reaction(FQRT-PCR).The results showed that after lead induction,the number of hippocampus neuron increased,cell gap increased,the chromatin was stained lightly;after administration of ABMP,the number of the hippocampus neuron increased as the dose of ABMP rose,cell gap decreased,the chromatin was stained darkly.The expression level of ASIC1a mRNA in hippocampus of rats in ABMP group decreased extremely significantly compared with that in the model group(P〈0.01).Lead exposure and ABMP exhibited no significant effects on the expression level of ASIC2b mRNA.ABMP possesses a protective effect on nervous system of rats exposed to lead by combining with lead. 展开更多
关键词 LEAD Agaricus blazei Murill polysaccharides HE staining QRT-PCR
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Study on the Cultivation of <i>Agaricus blazei</i>(Almond Mushroom) Grown on Compost Mixed with Selected Agro-Residues
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作者 Tun Tun Win Shoji Ohga 《Advances in Microbiology》 2018年第10期778-789,共12页
The Agaricus blazei strain (KUMB 1221) from Forest Production Control Laboratory, Kyushu University was grown on the basal media of compost mixed with selected agro-residues (sawdust, woodchips and corncob) in the rat... The Agaricus blazei strain (KUMB 1221) from Forest Production Control Laboratory, Kyushu University was grown on the basal media of compost mixed with selected agro-residues (sawdust, woodchips and corncob) in the ratio of 25%, 50%, 75% and 100% (by weight). The data was collected on the colony diameter of mycelial growth, days required for spawn run (colonization), days required for primordial formation, whiteness measurement, days required for fruit body formation, number, size and fresh weight of mushrooms, biological efficiency (BE) and mushroom production (MP). It was observed that the mycelial growth showed two types of mycelia according the level of mycelial density;compact (C) and somewhat compact (SC). The fastest spawn run (17 days) was found in woodchips (50%) among all the different treatments while the least spawn run took 26 days in corncob (75%). Sawdust substrates promoted longer days for primordial formation and fruit body development, if compared with woodchips substrates and corncob substrates. With regard to yield, 100%, 75% and 50% mixture with compost were superior to 25% mixture with compost in each group of selected agro-residues and it indicated that adding more compost gave the increased yield. Interestingly, it was noted that mushroom size on compost (100%) was double to the mushroom size of other treatments. In conclusion, it was clearly showed that compost (100%), woodchips (25%) and corncob (25%) could produce better yields among all treatments, and it was also possible to obtain acceptable yields of good quality almond mushroom using main substrates of compost mixed with different agro-residues at various concentrations. 展开更多
关键词 AGARICUS blazei MUSHROOM CULTIVATION Agro-Residues COMPOST Substrate
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Study on Anti-infection Effect of Polysaccharide from Agaricus blazei Murrill on Chickens
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作者 Chao REN Shuifeng XIAN +4 位作者 Yuqing ZHOU Jie LI Deliang WEI Nina JIA Shunzi YUAN 《Agricultural Biotechnology》 CAS 2018年第5期105-107,111,共4页
In order to study the anti-bacterial infection effect of polysaccharide from Agaricus blazei Murrill on chickens, the experimental groups were orally administrated A. blazei polysaccharide at low dose and high dose, r... In order to study the anti-bacterial infection effect of polysaccharide from Agaricus blazei Murrill on chickens, the experimental groups were orally administrated A. blazei polysaccharide at low dose and high dose, respectively, for 14 d continuously, and then, the chickens in various groups were infected with Escherichia coli or Pasteurella pneumotropica , so as to observe the clinical symptoms of chickens and record the change in body weight. Anatomy was performed 14 d later, and the organ indices were determined, so as to study the anti-bacterial infection effect of A. blazei polysaccharide on chickens. The results showed that after bacterial infection, the high-dose A. blazei polysaccharide group was significantly differed from other groups in changes of body weight and organ indices. It indicates that oral administration of high concentration of A. blazei polysaccharide could promote the development of poultry organs, thereby improving the immunity of organisms. 展开更多
关键词 Polysaccharide from Agaricus blazei Murrill Escherichia coli Pasteurella pneumotropica CHICKEN Body weight Immune organ index
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巴氏菇Agaricus blazeimurrill栽培新法初步研究
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作者 杨旭 《哈尔滨师范大学自然科学学报》 CAS 2000年第5期97-99,共3页
本文报导了以苷蔗渣为原料 ,不用粪肥 ,经栽培料二次发酵生产agaricus blazei murrill技术 .
关键词 甘蔗渣 二次发酵 栽培 巴氏菇
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姬松茸blazeispirol类成分的结构、活性及生物合成研究进展
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作者 丁婵 彭思源 +4 位作者 孟俊华 厉晓 龚婕 李云耀 崔培梧 《中国中药杂志》 CAS CSCD 北大核心 2024年第5期1144-1153,共10页
姬松茸是一种珍稀药食两用真菌,肉脆味美,其子实体与菌丝体均富含多糖、甾醇、萜类、多肽、脂质、多酚等活性成分,具有较强的抗肿瘤、降血脂、降血糖、免疫调节、优化肠道菌群、抗氧化等药理活性,是一种极具食用、药用开发前景的真菌资... 姬松茸是一种珍稀药食两用真菌,肉脆味美,其子实体与菌丝体均富含多糖、甾醇、萜类、多肽、脂质、多酚等活性成分,具有较强的抗肿瘤、降血脂、降血糖、免疫调节、优化肠道菌群、抗氧化等药理活性,是一种极具食用、药用开发前景的真菌资源。在已报道的姬松茸化学成分中,blazeispirols为姬松茸特有的一系列具有螺环结构且有别于经典甾体的甾醇化合物,是从姬松茸菌丝体中发现的重要活性成分,具有显著的保肝活性,可作为姬松茸菌株专属的系统发育和化学分类学标志,也被视为优秀的成药性先导化合物分子。依据骨架结构特征,可将目前已发现的17个blazeispirol类化合物分为blazeispirane和problazeispirane 2种类型。为进一步挖掘姬松茸特有blazeispirol类化合物资源,该文从姬松茸blazeispirol系列化合物的发现、分离、结构、生物活性和生物合成途径等角度进行了系统综述,同时以blazeispirol A为例总结了姬松茸发酵合成blazeispirol A的相关代谢调控策略,以期为blazeispirol系列化合物的高效合成与制备、相关药物及功能性食品的研发、姬松茸等食药真菌资源及其衍生产品的品质提升等相关研究提供参考。 展开更多
关键词 姬松茸 blazeispirol 甾醇 生物活性 生物合成
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食用菌姬松茸多糖的分离纯化及结构分析 被引量:1
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作者 耿敏 李晓婧 +3 位作者 王嘉怿 张春玉 周义发 张梦珊 《东北师大学报(自然科学版)》 CAS 北大核心 2024年第1期115-124,共10页
采用热水煮提、乙醇沉淀和离子交换-凝胶组合柱层析的方法,从食用菌姬松茸中提取分离纯化出了两种电荷和相对分子质量分布均一的姬松茸多糖级分WABM-N-a和WABM-A-b,对两种均一级分的结构进行了研究.结果表明:WABM-N-a级分以1,6-Galp构... 采用热水煮提、乙醇沉淀和离子交换-凝胶组合柱层析的方法,从食用菌姬松茸中提取分离纯化出了两种电荷和相对分子质量分布均一的姬松茸多糖级分WABM-N-a和WABM-A-b,对两种均一级分的结构进行了研究.结果表明:WABM-N-a级分以1,6-Galp构成主链骨架结构,同时存在较多的1,6-Glcp 1,3-Fucp结构和少量的1,4-Glcp,1,3-Glcp结构连接在Gal的O-3和/或O-4位上,且侧链上的Glc在O-3位上存在少量的分支.WABM-A-b级分为1,6-Glcp连接的葡聚糖结构,Glc在O-2,O-3和/或O-4位上可能均有分支,侧链结构可能为1,3-Glcp,1,4-Glcp,T-Glcp和1,6-Galp. 展开更多
关键词 食用菌 姬松茸多糖 分离纯化 结构分析
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杏鲍菇菌渣代料栽培对姬松茸不同潮次产量及品质的影响
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作者 陈华 林怡 +3 位作者 叶菁 刘朋虎 翁伯琦 王义祥 《农业环境科学学报》 CAS CSCD 北大核心 2024年第1期183-189,共7页
为探究杏鲍菇代料栽培姬松茸的适宜比例,通过床栽试验研究了杏鲍菇替代不同比例稻草对姬松茸J2和J37菌株不同潮次子实体产量、营养品质的影响。结果表明:随着替代比例的增加,姬松茸J2和J37子实体产量呈现先升后降的变化趋势,替代比例为... 为探究杏鲍菇代料栽培姬松茸的适宜比例,通过床栽试验研究了杏鲍菇替代不同比例稻草对姬松茸J2和J37菌株不同潮次子实体产量、营养品质的影响。结果表明:随着替代比例的增加,姬松茸J2和J37子实体产量呈现先升后降的变化趋势,替代比例为30%时产量最高,姬松茸J2产量可达2.038 kg·m^(-2),姬松茸J37产量可达2.267 kg·m^(-2)。两种姬松茸子实体产量主要集中于第一潮和第二潮,且杏鲍菇菌渣替代栽培处理前两潮产量占比均高于传统栽培配方。随着潮次增加,姬松茸J2和J37子实体中多糖、粗蛋白和氨基酸的质量分数总体呈降低的趋势,但杏鲍菇菌渣替代处理多糖、粗蛋白和氨基酸质量分数随潮次增加降低的幅度小于传统栽培配方。杏鲍菇菌渣替代处理姬松茸子实体中粗蛋白、氨基酸和多糖的质量分数分别比传统栽培配方提高2.42%~10.44%、4.09%~12.00%和11.07%~23.70%,其中替代比例为30%时营养品质最优。从生产成本分析,杏鲍菇菌渣替代可降低姬松茸栽培原料成本35.08%~54.00%。研究表明,适宜比例的杏鲍菇菌渣和养殖场垫料组合代料栽培姬松茸的产量和品质优且不同潮次间相对稳定,而且该方式可以有效降低栽培材料的投入成本,综合效益比较高。 展开更多
关键词 杏鲍菇菌渣 姬松茸 产量 品质
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