Alteration of Sex and Non-Sex Hormones and Distribution Features of Blood ABO System Groups among the Women with Uterine Body Tumors

Objectives: The aim of the investigation was to study the hormonal status (sex hormones: estradiol (E2), progesterone (P), testosterone (T);non-sex gonadotropic hormones-luteinizing hormone (LH) and follicle-stimulating hormone (FSH)) of women with benign and malignant tumors of uterine body in the reproductive, menopause and postmenopause periods. Also the distribution features of the blood ABO system phenotype groups and their link to the development of uterine body tumors have been studied. Methods: The determination of hormones was made by the enzyme analysis method (ELAIZA), provided by the proper ELAIZA kits. For the study of blood ABO system antigens, internationally recognized immunoserology methods were used. Results: Investigations revealed the increased level of E2 and T on the background of the reduced P in the blood of the women with uterine tumors in the reproductive, menopause and post-menopause period. As for gonadotropic hormones, the decreased levels of LH and FSH have also been detected. From the ABO system phenotype groups A(II) group had the highest frequency between the women with malignant uterine tumor in the reproductive age. O (I) phenotype group was the most frequent in case of menopause and post-menopause women with uterine malignant tumors. Conclusions: Hormonal imbalance creates good conditions for the proliferation of uterine tissues and hence causes the development of benign and malignant uterine tumors. The imbalance of the sex steroid and gonadotropic hormones in the blood of post-menopause women indicates on the genotoxic mechanism of cancer development on the background of age-related changes. A(II) group had the highest frequency between the reproductive age women with uterine malignant tumor, while O (I) group was the most frequent in case of menopause and post-menopause patients.

微柱凝胶检验法在RhD、ABO血型抗原鉴定中的应用价值分析

目的探讨微柱凝胶检验法在RhD、ABO血型抗原鉴定中的应用价值。方法120例需行RhD及ABO血型鉴定患者,将所有取得的血样分为两份,分别使用试管法及手工微柱凝胶免疫检验法(微柱凝胶检验法)对血型进行分析。比较两种检测方式的鉴定结果。结果120例患者血样经试管法及微柱凝胶检验法检测后,结果均发现ABO阳性112例(其中A型34例,B型33例,AB型11例,O型34例),ABO阳性率为93.33%;RhD阴性1例,RhD阴性率为0.83%。两种方法的抗原检测结果符合率为100.00%,两种方法检测血型结果比较差异无统计学意义(P>0.05)。结论微柱凝胶检验法应用在RhD、ABO血型抗原鉴定中,操作简便,准确率高,值得在临床中推广应用。

Significant association between ABO blood group and pancreatic cancer

AIM:To evaluate whether the ABO blood group is related to pancreatic cancer risk in the general population of the United States.METHODS:Using the University of Pittsburgh's clinicalpancreatic cancer registry,the blood donor database from our local blood bank (Central Blood Bank),and the blood product recipient database from the regional transfusion service (Centralized Transfusion Service) in Pittsburgh,Pennsylvania,we identified 274 pancreatic cancer patients with previously determined serological ABO blood group information.The ABO blood group frequency was compared between these patients and 708842 individual,community-based blood donors who had made donations to Pittsburgh's Central Blood Bank between 1979 and 2009.RESULTS:The frequency of blood group A was statistically significantly higher amongst pancreatic cancer patients compared to its frequency amongst the regional blood donors [47.63% vs 39.10%,odds ratio (OR)=1.43,P=0.004].Conversely,the frequency of blood group O was significantly lower amongst pancreatic cancer patients relative to the community blood donors (32.12% vs 43.99%,OR=0.60,P=0.00007).There were limited blood group B (n=38) and AB (n=17) pancreatic cancer patients;the overall P trend value comparing patient to donor blood groups was 0.001.CONCLUSION:The ABO blood group is associated with pancreatic cancer risk.Future studies should examine the mechanism linking pancreatic cancer risk to ABO blood group.

Relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China

AIM:To study the relationship between ABO blood groups and carcinoma of esophagus and cardia in Chaoshan inhabitants of China, which is a unique Littoral high-risk area of esophageal carcinoma in China. The poor communication and transportation in the past has made Chaoshan a relatively closed area and kept its culture and custure of old China thousand years ago. METHODS: Data on age, sex, ABO blood type and X-ray or pathological diagnose of the patients with carcinoma of esophagus or cardia were collected from the Tumor Hospital. First Affiliated Hospital, Second Affiliated Hospital of Shantou University Medical College; and the Central Hospital of Shantou and the Central Hospital of Jieyang. A total of 6685 patients with esophageal carcinoma (EC) and 2955 patients with cardiac cancer (CC) in Chaoshan district were retrospectively assessed for their association with ABO blood groups. RESULTS: The distribution of ABO blood groups in patients with EC or CC was similar to the normal local population in Chaoshan. However, blood group B in male patients with CC and in the patients with carcinoma in the upper third esophagus was 2.3% and 4.7% higher than the corresponding controls. The relative risk B O was 1.1415 (P【0.05) and 1.2696 (P【0.05), respectively. No relationship was found between ABO blood groups and tumor differentiation. CONCLUSION: ABO blood group B is associated with the incidence of CC in male individuals and carcinoma in the upper third esophagus. The distribution of ABO blood groups varies in the different geographical and ethnic groups. As a result, proper controls are very important for such studies.

Challenge to ABO blood type barrier in living donor liver transplantation

ABO incompatible living donor liver transplantation has the potential to expand the donor pool for patients with end stage liver diseases on the expense of challenges to overcome immunological barriers across blood type.There is a profound impact of age on incidence and severity of antibody mediated rejection(AMR).Even children older than 1 year have chances of AMR;children aged 8 years or older have risks of hepatic necrosis similar to adult liver recipients.The mechanism of AMR is based on circulatory disturbances secondary to inflammation and injury of the vascular endothelium caused by an antibody-antigen-complement reaction.The strategy to overcome ABO blood type barrier is based on both pre-transplant desensitization and adequate treatment of this phenomenon.Nowadays,rituximab is the standard means of desensitization but unfortunately an insufficient aid to treat AMR.Because of low incidence(less than 5%in the rituximab era),in practice of AMR only some case reports about the treatment of clinical AMR are available in the literature.Initial experiences revealed that the proteasome inhibitor,bortezomib might be a promising treatment based on its capacity to deplete plasma cell agents.Although ABO blood type barrier has been counteracted in 95%of patients by applying“rituximab-desensitization”,many issues,such as prediction of high-risk patients of infection and AMR and secure treatment strategies for evoked AMR,remain to be resolved.

ABO blood group is a predictor of survival in patients with laryngeal cancer

Background: Whether the ABO blood group is associated with the survival of patients with laryngeal cancer remains unknown. The purpose of this study was to investigate the association between the ABO blood group and clinico?pathologic characteristics of patients with laryngeal cancer and assess whether the ABO blood group was associated with prognosis.Methods: We analyzed the records of 1260 patients with laryngeal cancer who underwent curative treatment at Sun Yat?sen University Cancer Center between January 1993 and December 2009. The Chi?square test was used to assess the relationship between the ABO blood group and clinicopathologic characteristics. The Kaplan–Meier method was used to estimate 3?, 5?, and 10?year overall survival(OS) rates. The Cox proportional hazards model was used in univariate and multivariate analyses of OS.Results: No signiicant association was found between the ABO blood group and clinicopathologic characteristics except for primary tumor site. The median OS for patients with blood groups A, B, AB, and O were 87.0, 80.0, 90.0, and 72.5 months, respectively. The 3?, 5?, and 10?year OS rates were 82.4%, 76.0%, and 67.5% for patients with blood group A; 77.4%, 69.8%, and 58.4% for patients with blood group B; 82.2%, 73.1%, and 65.6% for patients with blood group AB; and 71.7%, 66.4%, and 55.5% for patients with blood group O, respectively. Univariate and multivariate analyses showed that the ABO blood group had signiicant efects on prognosis in patients with laryngeal cancer.Conclusions: The ABO blood group is associated with survival in patients with laryngeal cancer. Patients with blood group O had signiicantly shorter OS than patients with other ABO blood groups.

Association of ABO blood group and Plasmodium falciparum malaria in Dore Bafeno Area,Southern Ethiopia

Objective:To assess the distribution of ABO blood group and their relationship with Plasmodium falciparum(P.falciparum) malaria among febrile outpatients who sought medical attention at Dore Bafeno Health Center,Southern Ethiopia.Methods:A total of 269 febrile outpatients who visited Dore Bafeno Health Center,Southern Ethiopia,were examined for malaria and also tested for ABO blood groups in January 2010.The blood specimens were collected by finger pricking,stained with Geimsa,and examined microscopically.Positive cases of the parasitemia were counted.CareStart^(TM) Malaria PflPv Combo was also used to test the blood specimens for malaria.ABO blood groups were determined by agglutination test using ERYCLONE antisera.Data on socio-demographic characteristics and treatment status of the participants were also collected.Chi-square and ANOVA tests were used to assess the difference between frequencies and means,respectively.Results:Out of a total of 269 participants,178(66.2%) febrile patients were found to be infected with Plasmodium parasites,among which 146(54.3%),28(10.4%),and 4(1.5%) belonged to P.falciparum,P.vivax,and mixed infections,respectively.All febrile patients were also tested for ABO blood groups and 51.3%,23.5%,21.9%and 3.3%were found to be blood types of 0,A,B and AB,respectively.Both total malaria infection and P.falciparum infection showed significant association with blood types(P<0.05).The proportion of A or B but not 0 phenotypes was higher(P<0.05) in individuals with P.falciparum as compared with non-infected individuals.The chance of having P.falciparum infection in patients with blood groups A,B and AB was 2.5,2.5 and 3.3times more than individuals showing blood 0 phenotypes,respectively.The mean P.falciparum malaria parasitemia for blood groups A,B,AB,and 0 were 3 744/μ L,1 805/ μ L,5 331/μ L,and1 515/μ L,respectively(P<0.01).Conclusions:The present findings indicate that individuals of blood groups A,B and AB are more susceptible to P.falciparum infection as compared with individuals of blood group O.Nevertheless,further in depth studies are required to clearly establish the role that ABO blood group plays in P.falciparum malaria.

ABO血型次要不合的异基因造血干细胞移植合并过客淋巴细胞综合征患者的诊治

目的探讨异基因造血干细胞移植(allogeneic hematopoietic stem cell transplantation,allo-HSCT)患者发生过客淋巴细胞综合征(passenger lymphocyte syndrome,PLS)的临床特点和诊治方法。方法回顾2018年12月至2022年12月在苏州弘慈血液病医院行allo-HSCT的489名患者。分析移植后并发PLS的4名患者的临床过程、诊治措施及预后。结果489名allo-HSCT患者中,4例确诊为PLS,供受体ABO血型均为次要不合(O供A或B)。PLS在allo-HSCT中的总体发生率为0.82%(4/489),在供受体ABO血型次要不合的移植中发生率为2.2%(4/179)。PLS发生在供体干细胞回输6~13 d,临床表现为头晕乏力明显,腰痛、黄疸、尿色加深,实验室检查血红蛋白迅速下降,间接胆红素、乳酸脱氢酶升高,尿胆原阳性、直接抗人球蛋白试验(DAT)阳性,并且血浆中均检测出针对受者红细胞的抗-A或抗-B。治疗上予以输注O型洗涤红细胞,甲强龙,丙种球蛋白、利妥昔单抗等治疗后患者溶血表现改善。所有患者都获得了粒系、血小板植入,并且红细胞脱离输注时间均在3周内。结论PLS是异基因造血干细胞移植少见的并发症,主要发生在ABO血型次要不合的allo-HSCT患者中,及时处置临床预后良好。

先天性腹膜鞘状突未闭疾患与ABO血型的相关性

目的探讨先天性腹膜鞘状突未闭(PPV)疾患与ABO血型的相关性。方法回顾性分析2003年1月至2021年12月该院收治的1128例存在PPV疾患儿童(病例组)的临床资料和同期收治的4208例其他疾病儿童(对照组)的临床资料。病例组根据疾病类型分为腹股沟斜疝组(738例)、精索鞘膜积液组(228例)和隐睾组(162例)。比较各组患儿ABO血型分布情况。结果腹股沟斜疝组患儿中A、B、O、AB型血分别占28.3%(209/738)、28.5%(210/738)、28.7%(212/738)、14.5%(107/738),对照组患儿中A、B、O、AB型血分别占29.9%(1257/4208)、29.4%(1237/4208)、22.6%(949/4208)、18.2%(765/4208),2组患儿血型构成比较,差异有统计学意义(P<0.05)。A、B、AB型血儿童较O型血儿童患腹股沟斜疝风险低[优势比(OR)=0.744、0.760、0.626,95%可信区间(95%CI):0.604~0.917、0.617~0.936、0.487~0.805,P=0.006、0.010、<0.001]。调整年龄、性别、民族等影响因素后O型血儿童患腹股沟斜疝的风险较非O型血高(OR=1.396,95%CI:1.160~1.679,P<0.001),AB型血儿童患腹股沟斜疝的风险较非AB型血低(OR=0.742,95%CI:0.590~0.934,P=0.011)。结论该院所诊治的儿童病例中因先天性PPV发生腹股沟斜疝与ABO血型可能存在相关性,ABO血型为小儿腹股沟斜疝发生的一个影响因素。

The correlation between different ABO blood group gene loci and the pathogenesis and prognosis of acute myocardial infarction

Objective: To assess the effect of different ABO blood group gene locus on severity and prognosis of acute myocardial infarction (AMI). Methods: 100 patients with AMI diagnosed in our hospital from June 2018 to June 2019 were selected as the myocardial infarction group. At the same time, 100 healthy patients with physical examination results in our hospital were selected as the control group. Single-nucleotide polymorphisms of peripheral blood ABO gene loci rs505922, rs579459, rs643434, rs651007, and rs8176743 were detected. The differences of ABO blood group gene loci in different groups were compared. The correlation between the distribution of ABO blood group gene loci and the incidence and prognosis of patients with AMI was analyzed. Results: 98 cases were included in the myocardial infarction group and 99 cases were included in the control group. Compared with the control group, the genotypes of rs643434 and rs651007 locus in the myocardial infarction group were statistically significant (P<0.05). Analysis under different genetic models , compared with the control group, the myocardial infarction group had a significant difference in the rs643434 gene dominant mode, rs651007 dominant mode and recessive mode (P<0.05). The dominant mode of rs643434 was correlated with Gensini score and TIMI grade. The dominant mode of rs651007 was correlated with Gensini score, TIMI grade and troponin.The recessive mode of rs651007 was correlated with Gensini score, and the differences were statistically significant (P < 0.05). The dominant mode of rs643434 was related to the occurrence of MACE, and the difference was statistically significant (P < 0.05).Conclusion: Different genetic models of ABO blood group genes rs643434 and rs651007 may be related to the condition and prognosis of AMI.

肠道菌群与人类ABO血型

肠道菌群因与人类健康和疾病密切相关而成为当前生命科学和医学领域的研究热点。近年来,各国多个权威实验室先后报道了肠道菌群与人类ABO血型之间的相互作用。本文首先将对人类肠道菌群的概念、组成、结构及生理功能进行简要介绍,分析肠道菌群相关研究的趋势。其次,将基于现有的研究成果对肠道菌群与人类血型之间的关系进行简要论述,既包含肠道菌群对于人类血型的改变,也包括人类血型对于肠道菌群组成结构的影响。最后,分析当前关于肠道菌群与人类血型的研究成果如何更好地应用于输血科研和输血治疗领域。

ABO Blood Groups and Their Relationship with Coagulation Factor VIII in Healthy Adults

Background: ABO blood group distribution defers with racial and geographic variations. They are related to diseases like cardiovascular diseases, cerebral thromboembolism. ABO blood group system may influence coagulation factor VIII which may increase the future risk of thrombosis. Aim: To assess the relation of ABO blood group with coagulation factor VIII in healthy adults. Material and Methods: A prospective type of analytical cross-sectional study was conducted in the Department of Physiology, Dhaka Medical College, Dhaka from July 2019 to June 2020. After obtaining ethical clearance, a total of 190 healthy adults were selected from different areas of Dhaka city based on inclusion and exclusion criteria, with ages ranging from 18 - 45 years. The subjects were interviewed and detailed history regarding personal, family, medical and drug were taken. Prior to sample collection, informed written consent was taken from the participants. Individuals of blood group A were selected as group A, blood group B as group B, blood group AB as group AB and blood group O as group O. Coagulation factor VIII was measured in the Department of Hematology and BMT Unit, Dhaka Medical College Hospital, Dhaka. Blood grouping was done in the Department of Physiology, Dhaka Medical College, Dhaka. Statistical Analysis: For statistical analysis, ONE way ANOVA followed by Bonferroni test were considered using SPSS 25.0 version. Results: In this study, blood group B was most common (33.2%). Coagulation factor VIII was significantly higher (p < 0.001) in blood group A (105.76% ± 11.82%), B (112.00% ± 15.02%), AB (109.80% ± 11.93%) than blood group O (82.00% ± 12.86%). No significant difference was observed among A, B and AB blood groups regarding coagulation factor VIII. Conclusions: It can be concluded that blood group A, B, AB individuals may have more chance of thrombosis due to significantly higher coagulation factor VIII than blood group O individuals.

ABO疑难血型的原因及鉴定方法分析

目的分析ABO疑难血型的原因及鉴定方法,确保临床输血安全。方法应用ABO疑难血型三步分析法,对本院2020年6月至2022年12月本院接收的110例ABO正反定型不一致标本进行分析鉴定。结果110例ABO正反定型不一致标本中,人为因素造成的有18例(16.36%);由于疾病本身或治疗因素造成的有92例(83.64%)。人为因素中,人员技术问题占比27.78%、未按规程操作占比22.22%、标本污染占比22.22%、标本标签错误占比11.11%、离心机问题占比11.11%、试剂过期占比5.56%。92份因疾病本身或治疗因素中,抗原因素28例,占比30.43%;抗体因素64例,占比67.57%。结论应用ABO疑难血型三步分析法能快速有效的解决疑难血型的鉴定,确保临床输血安全。

ABO血型对健康年轻国人周围神经传导的影响

目的 探讨ABO血型对周围神经传导产生的影响,为周围神经病的预防及治疗提出新思路和方法。方法 选择延边大学医学院在校健康青年志愿者856名,检测所有研究对象ABO血型,及正中、尺、腓、胫神经神经传导参数和F波,分析不同血型间的神经传导差异。结果 与左侧相比,正中神经O型传导机能右侧降低,尺神经B、O型波幅右侧升高,非B型腓神经传导速度右侧升高,胫神经O型远位潜伏期/波幅(基-峰)右侧延长/升高(P均<0.05)。正中和胫神经传导机能AB型优于非AB型;腓神经传导机能非O型优于O型;各神经F波最短潜伏期O型较非O型延长(P均<0.05)。超过可信区上限或低于下限的比率:正中和尺神经AB型小于非AB型,腓和胫神经A型小于非A型(P均<0.05)。神经传导机能显著低下神经数尺、腓、胫神经中O型显著。结论 神经传导机能AB型优于非AB型;神经传导低下比率O型明显,左侧与右侧传导差异O型最明显。

Serum anti‑blood type IgG/IgM binding and cytotoxicity to pig PBMC and RBC

Objective: TO investigate the killing differences of human blood group antibodies IgG and IgM on PBMC and RBC in peripheral blood of genetically modified pigs, and provide theoretical basis for the selection of pig blood group for clinical application of porcine xenotransplantation. Methods: Serum samples were collected from 20 healthy subjects, 20 patients with end‑stage renal disease and 20 brain dead organ donors, and divided into 4 groups on the basis of ABO blood group (A: n=20;B: n=17;AB: n=7;O: n=16). Flow cytometry was used to detect antibody binding or complement dependent cytotoxicity test (CDC) between human serum and O blood group Wild‑type (WT), α1, 3‑galactosyltransferase gene‑knockout(GTKO), cytidine monophosphate‑N‑acetylneuraminic acid hydroxylase gene‑knockout (GTKO/β4GalNT2KO), β‑1, 4N‑acetylgalactosaminyltransferase gene‑knockout(GTKO/CMAHKO) and mononuclear cells (PBMC) and red blood cells (RBC) of Triple knockout (TKO/hCD55) porcine peripheral blood, respectively. Results: There was no significant difference in binding and killing of human serum antibodies with blood group A, B, AB and O on PBMC of WT pigs, GTKO pigs, GTKO/β4GalNT2KO pigs, GTKO/CMAHKO pigs and TKO/hCD55 pigs, respectively. There was no significant difference in RBC binding with RBC of WT pig, GTKO pig, GTKO/β4GalNT2KO pig, GTKO/CMAHKO pig and TKO/hCD55 pig. Conclusion: The selection of recipients of pigs with type O blood group can be done without considering blood group.

一例ABO血型A抗原减弱的血清学及其基因变异分析

目的分析1例A抗原表达减弱患者的血清学及分子生物学特点,并探讨其分子生物学机制及输血策略。方法应用常规血清学方法进行ABO血型鉴定,测序分型确定ABO基因型。结果血清学格局为A抗原减弱,基因测序表明外显子6存在c.261delG杂合突变、外显子7存在c.467C>T杂合突变、内含子6存在c.374+5G>A杂合突变,患者基因型为A102/AEL04/O01,表型为Ael。结论A等位基因内含子6的c.374+5G>A突变,导致转录过程形中成不同剪接变异体,造成A型糖基转移酶的活性减弱或失活,是本例患者A抗原减弱的主要原因。

ABO血型不合对异基因造血干细胞移植的影响

目的:分析ABO血型不合对异基因造血干细胞移植(allo HSCT)的影响。方法: 对北京大学人民医院血液病研究所 148例ABO血型不合的allo HSCT受者按不同的移植方式进行分析,并选用同期 85例ABO血型相合的受者作比较。结果:主要ABO血型不合骨髓移植(BMT)组红细胞输注量多,红系恢复时间长。ABO血型不合的BMT、外周血干细胞移植(PBSCT)以及BMT+PBSCT患者急性移植物抗宿主病 (aGVHD)的发生率、巨细胞病毒(CMV)发生率及 5年无病存活率与ABO血型相合患者相比差异无统计学意义。结论:ABO血型不合不影响造血干细胞移植的植活、主要合并症及预后。ABO血型不合的PBSCT与BMT相比,红细胞输注量少,红系恢复较快。