In this paper, a non-Newtonian third-grade blood in coronary and femoral arteries is simulated analytically and numerically. The blood is considered as the third- grade non-Newtonian fluid under the periodic body acce...In this paper, a non-Newtonian third-grade blood in coronary and femoral arteries is simulated analytically and numerically. The blood is considered as the third- grade non-Newtonian fluid under the periodic body acceleration motion and the pulsatile pressure gradient. The hybrid multi-step differential transformation method （Hybrid-Ms- DTM） and the Crank-Nicholson method （CNM） are used to solve the partial differential equation （PDE）, and a good agreement between them is observed in the results. The effects of the some physical parameters such as the amplitude, the lead angle, and the body acceleration frequency on the velocity and shear stress profiles are considered. The results show that increasing the amplitude, Ag, and reducing the lead angle of body acceleration, 9, make higher velocity profiles on the center line of both arteries. Also, the maximum wall shear stress increases when Ag increases.展开更多
目的探究个体化饮食指导在糖尿病骨性关节炎患者中的应用价值。方法选取晋江市医院(上海市第六人民医院福建医院)于2023年2—10月收治的90例糖尿病骨性关节炎患者为研究对象,采用密闭信封法分为对照组和研究组,各45例。对照组实施常规干...目的探究个体化饮食指导在糖尿病骨性关节炎患者中的应用价值。方法选取晋江市医院(上海市第六人民医院福建医院)于2023年2—10月收治的90例糖尿病骨性关节炎患者为研究对象,采用密闭信封法分为对照组和研究组,各45例。对照组实施常规干预,研究组结合个体化饮食指导。于干预前及干预2个月后,检测两组空腹血糖(Fasting Plasma Glucose,FPG)、餐后2 h血糖(2 h Postprandial Plasma Glucose,2 hPG)水平。对比两组负性情绪及生活质量简表(World Health Organization Quality of Life 100,WHOQOL-100)评分。结果干预2个月后,研究组FPG水平为(5.34±0.51)mmol/L,2 hPG水平为(6.73±0.56)mmol/L,均低于对照组,差异有统计学意义(t=5.348、10.807,P均<0.05)。研究组负性情绪评分低于对照组,WHOQOL-100各维度评分均高于对照组,差异有统计学意义(P均<0.05)。结论糖尿病骨性关节炎患者行个体化饮食指导干预,可有效降低血糖水平,缓解负性情绪,从而提高患者生活质量。展开更多
Most studies on spinal cord neuronal injury have focused on spinal cord tissue histology and the expression of nerve cell damage and repair-related genes. The importance of the microcirculation is often ignored in spi...Most studies on spinal cord neuronal injury have focused on spinal cord tissue histology and the expression of nerve cell damage and repair-related genes. The importance of the microcirculation is often ignored in spinal cord injury and repair research. Therefore, in this study, we established a rat model of intervertebral disc extrusion by inserting a silica gel pad into the left ventral sur-face of T13. Electroacupuncture was used to stimulate the bilateralZusanlipoint （ST36） and Neiting point （ST44） for 14 days. Compared with control animals, blood lfow in the ifrst lumbar vertebra （L1） was noticeably increased in rats given electroacupuncture. Microvessel density in the T13 segment of the spinal cord was increased significantly as well. The number of normal neurons was higher in the ventral horn of the spinal cord. In addition, vacuolation in the white matter was lessened. No obvious glial cell proliferation was visible. Furthermore, hindlimb motor function was improved signiifcantly. Collectively, our results suggest that electroacupuncture can improve neuronal morphology and microcirculation, and promote the recovery of neurological functions in a rat model of intervertebral disc extrusion.展开更多
Blood pressure and sleep,the two important indicators of health outcomes,are of increasing public health importance.There are variations in the blood pressure parameters during sleep and association exists between sle...Blood pressure and sleep,the two important indicators of health outcomes,are of increasing public health importance.There are variations in the blood pressure parameters during sleep and association exists between sleep and elevated blood pressure.The absence of nocturnal dipping of the blood pressure also increases cardiovascular risk.Sleep duration has been defined as normal(7-8 hours of sleep),short≤6 hours and long sleep≥9 hours.Short sleep duration is associated with hypertension especially during middle age and women who are deprived of sleep particularly post-menopausal had a higher risk of hypertension than men.Methodological concerns exist in the measurement of sleep duration,although both subjective and objective assessment of sleep duration showed an association with hypertension.Shorter sleep duration is believed to result in prolonged exposures to elevated sympathetic activity and psychosocial stressors.There is no enough evidence to support this statement for longer sleep duration.Long and short sleep duration are associated with the incidence of hypertension across most age groups.Relationships of short sleep are stronger among women and young adults compared to long sleep duration which is stronger among the elderly.展开更多
目的探讨炎症条件的动物输注贮存红细胞对巨噬细胞(BMDMs)的调节作用以及贮存红细胞输注与细菌感染引发炎症反应的关系。方法将6~8周龄成年雄性C57BL/6小鼠[(18~22)g/只]40只随机均分为实验组和实验对照组(对照组),均通过动物尾静脉注...目的探讨炎症条件的动物输注贮存红细胞对巨噬细胞(BMDMs)的调节作用以及贮存红细胞输注与细菌感染引发炎症反应的关系。方法将6~8周龄成年雄性C57BL/6小鼠[(18~22)g/只]40只随机均分为实验组和实验对照组(对照组),均通过动物尾静脉注射铜绿假单胞菌200μL/只,并使用吸入式麻醉剂异氟烷(1%~3%)麻醉后,通过小鼠眼后静脉丛,实验组输注鼠源贮存悬浮红细胞(>14 d)400μL/只、对照组每只输注等量新鲜悬浮红细胞(贮存<24 h);于输注后2、4、8 h脱就猝死各结束2组小鼠生命5只,摘取鼠肝,体外培养铜绿假单胞菌感染(200μL/只)小鼠的股骨、胫骨骨髓来源的BMDMs,流式细胞术检测BMDMs中分化簇86(CD86)、分化簇197(CD197)[巨噬细胞1型(M1)基因特异性标志物]、分化簇209(CD209)[巨噬细胞2型(M2)基因特异性标记]表达水平,实时荧光定量PCR(qRT-PCR)法检测小鼠肝脏F4/80、M1、M2基因表达水平,并使用SPSS17.0统计学软件分析数据。结果实验组与对照组BMDMs中CD86和CD197的表达(%)分别为8688±1.01 vs 79.24±2.65、38.59±3.73 vs 25.95±0.86(P<0.05),CD209(%)为23.88±2.23 vs 21.91±3.58(P>0.05)。输注红细胞后2、4 h,小鼠肝F4/80基因表达水平实验组和对照组分别为1.83±0.11 vs 0.75±0.06、0.46±0.06 vs 0.33±0.06(P<0.05),8 h后分别为0.33±0.03 vs 0.35±0.05(P>0.05);输注红细胞2、4、8 h,小鼠肝M1基因中诱导型一氧化氮合酶(iNOS)基因表达水平实验组和对照组分别为3.44±0.20 vs 2.46±0.08、9.25±0.55 vs 2.67±0.12、2.80±0.08 vs 2.39±0.01,肿瘤坏死因子-α(TNF-α)分别为1.69±0.22 vs 1.13±0.03、1.44±0.24 vs 0.96±0.09、1.31±0.05 vs 0.96±0.06,单核细胞趋化蛋白1(MCP1)分别为4.96±0.08 vs 4.28±0.27、4.63±0.04 vs 2.07±0.09、2.28±0.19 vs 1.33±0.03(P<0.05);M2基因中精氨酸1(Arg1)基因表达水平实验组和对照组分别为0.81±0.21 vs 0.82±0.18、0.66±0.11 vs 0.58±0.09、0.39±0.17 vs 0.37±0.15,甘露糖受体C型2(Mrc2)分别为0.99±0.91 vs 0.97±0.08、0.98±0.12 vs 1.02±0.11、0.59±0.19 vs 0.57±0.08,重组蛋白163(CD163)分别为1.75±0.20 vs 1.69±0.18、0.22±0.02 vs 0.21±0.01、0.04±0.01 vs 0.03±0.01(P>0.05)。结论实验小鼠输注贮存红细胞明显促进其肝脏组织巨噬细胞朝向M1表型的极化。展开更多
目的考察滤除白细胞对全血保存质量和功能的影响。方法 1袋(2 U=400 mL)新鲜全血均分为滤除白细胞全血(简称滤白)组:按照去白滤器的使用说明书操作,在采血<6 h过滤制备去白细胞全血;对照组:不滤除白细胞。过滤及未过滤的全血均采用...目的考察滤除白细胞对全血保存质量和功能的影响。方法 1袋(2 U=400 mL)新鲜全血均分为滤除白细胞全血(简称滤白)组:按照去白滤器的使用说明书操作,在采血<6 h过滤制备去白细胞全血;对照组:不滤除白细胞。过滤及未过滤的全血均采用无菌方式分别分为6小袋[(30～40)mL/袋],(4±2)℃常规保存,分别于保存1、7、14、21、28、35 d各取1小袋,检测血常规、pH、游离血红蛋白(FHb)、氧亲和力(P50)、ATP、2,3-二磷酸甘油酸(2,3-DPG)、电解质、红细胞膜表面PS、CD47表达、葡萄糖、乳酸等指标,比较过滤与未过滤全血质量的差异。结果实验组1 U全血过滤后剩余白细胞数为(0.32±0.05)×106个;滤白组和对照组比较,保存35 d时血红蛋白(Hb)(g/L)为149.00±11.53 vs 137.20±3.96、MCV(fL)为98.90±3.23 vs 98.96±2.10、pH为6.78±0.02 vs6.75±0.02、P50(mmHg)为15.36±0.63 vs 15.18±0.22、ATP(μmol/gHb)为3.57±0.15 vs 3.61±1.02、2,3-DPG(mmol/L)为0.13±0.09 vs 0.22±0.2、PS阳性率(%)为0.96±0.08 vs 1.19±0.03、CD47阳性率(%)为47.65±3.92 vs 48.37±4.35(P>0.05),溶血率(%)为0.08±0.02 vs 0.12±0.03(P<0.05);保存28 d时乳酸(mmol/L)为15.76±0.19 vs 18.73±0.97、葡萄糖(mmol/L)为20.21±0.55 vs 18.57±0.46(P<0.05)。结论全血离体保存期间会发生一系列质量和功能的变化,滤除白细胞全血的溶血率、乳酸、葡萄糖等指标略优于未过滤的全血,二者的携释氧功能及其他生理生化指标均无明显差异,说明滤除白细胞未对全血保存期间的质量产生不良影响,并可能有利于血液保存。展开更多
文摘In this paper, a non-Newtonian third-grade blood in coronary and femoral arteries is simulated analytically and numerically. The blood is considered as the third- grade non-Newtonian fluid under the periodic body acceleration motion and the pulsatile pressure gradient. The hybrid multi-step differential transformation method （Hybrid-Ms- DTM） and the Crank-Nicholson method （CNM） are used to solve the partial differential equation （PDE）, and a good agreement between them is observed in the results. The effects of the some physical parameters such as the amplitude, the lead angle, and the body acceleration frequency on the velocity and shear stress profiles are considered. The results show that increasing the amplitude, Ag, and reducing the lead angle of body acceleration, 9, make higher velocity profiles on the center line of both arteries. Also, the maximum wall shear stress increases when Ag increases.
文摘目的探究个体化饮食指导在糖尿病骨性关节炎患者中的应用价值。方法选取晋江市医院(上海市第六人民医院福建医院)于2023年2—10月收治的90例糖尿病骨性关节炎患者为研究对象,采用密闭信封法分为对照组和研究组,各45例。对照组实施常规干预,研究组结合个体化饮食指导。于干预前及干预2个月后,检测两组空腹血糖(Fasting Plasma Glucose,FPG)、餐后2 h血糖(2 h Postprandial Plasma Glucose,2 hPG)水平。对比两组负性情绪及生活质量简表(World Health Organization Quality of Life 100,WHOQOL-100)评分。结果干预2个月后,研究组FPG水平为(5.34±0.51)mmol/L,2 hPG水平为(6.73±0.56)mmol/L,均低于对照组,差异有统计学意义(t=5.348、10.807,P均<0.05)。研究组负性情绪评分低于对照组,WHOQOL-100各维度评分均高于对照组,差异有统计学意义(P均<0.05)。结论糖尿病骨性关节炎患者行个体化饮食指导干预,可有效降低血糖水平,缓解负性情绪,从而提高患者生活质量。
基金supported by the National Natural Science Foundation of China,No.31372473,30871886the Funding Project for Academic Human Resources Development in Institutions of Higher Learning under the Jurisdiction of Beijing Municipality of China,No.PHR201107134the 2012 Scientific Research Quality Raising Funds of Beijing University of Agriculture of China,No.PXM2012_014207_000010
文摘Most studies on spinal cord neuronal injury have focused on spinal cord tissue histology and the expression of nerve cell damage and repair-related genes. The importance of the microcirculation is often ignored in spinal cord injury and repair research. Therefore, in this study, we established a rat model of intervertebral disc extrusion by inserting a silica gel pad into the left ventral sur-face of T13. Electroacupuncture was used to stimulate the bilateralZusanlipoint （ST36） and Neiting point （ST44） for 14 days. Compared with control animals, blood lfow in the ifrst lumbar vertebra （L1） was noticeably increased in rats given electroacupuncture. Microvessel density in the T13 segment of the spinal cord was increased significantly as well. The number of normal neurons was higher in the ventral horn of the spinal cord. In addition, vacuolation in the white matter was lessened. No obvious glial cell proliferation was visible. Furthermore, hindlimb motor function was improved signiifcantly. Collectively, our results suggest that electroacupuncture can improve neuronal morphology and microcirculation, and promote the recovery of neurological functions in a rat model of intervertebral disc extrusion.
文摘Blood pressure and sleep,the two important indicators of health outcomes,are of increasing public health importance.There are variations in the blood pressure parameters during sleep and association exists between sleep and elevated blood pressure.The absence of nocturnal dipping of the blood pressure also increases cardiovascular risk.Sleep duration has been defined as normal(7-8 hours of sleep),short≤6 hours and long sleep≥9 hours.Short sleep duration is associated with hypertension especially during middle age and women who are deprived of sleep particularly post-menopausal had a higher risk of hypertension than men.Methodological concerns exist in the measurement of sleep duration,although both subjective and objective assessment of sleep duration showed an association with hypertension.Shorter sleep duration is believed to result in prolonged exposures to elevated sympathetic activity and psychosocial stressors.There is no enough evidence to support this statement for longer sleep duration.Long and short sleep duration are associated with the incidence of hypertension across most age groups.Relationships of short sleep are stronger among women and young adults compared to long sleep duration which is stronger among the elderly.
文摘目的探讨炎症条件的动物输注贮存红细胞对巨噬细胞(BMDMs)的调节作用以及贮存红细胞输注与细菌感染引发炎症反应的关系。方法将6~8周龄成年雄性C57BL/6小鼠[(18~22)g/只]40只随机均分为实验组和实验对照组(对照组),均通过动物尾静脉注射铜绿假单胞菌200μL/只,并使用吸入式麻醉剂异氟烷(1%~3%)麻醉后,通过小鼠眼后静脉丛,实验组输注鼠源贮存悬浮红细胞(>14 d)400μL/只、对照组每只输注等量新鲜悬浮红细胞(贮存<24 h);于输注后2、4、8 h脱就猝死各结束2组小鼠生命5只,摘取鼠肝,体外培养铜绿假单胞菌感染(200μL/只)小鼠的股骨、胫骨骨髓来源的BMDMs,流式细胞术检测BMDMs中分化簇86(CD86)、分化簇197(CD197)[巨噬细胞1型(M1)基因特异性标志物]、分化簇209(CD209)[巨噬细胞2型(M2)基因特异性标记]表达水平,实时荧光定量PCR(qRT-PCR)法检测小鼠肝脏F4/80、M1、M2基因表达水平,并使用SPSS17.0统计学软件分析数据。结果实验组与对照组BMDMs中CD86和CD197的表达(%)分别为8688±1.01 vs 79.24±2.65、38.59±3.73 vs 25.95±0.86(P<0.05),CD209(%)为23.88±2.23 vs 21.91±3.58(P>0.05)。输注红细胞后2、4 h,小鼠肝F4/80基因表达水平实验组和对照组分别为1.83±0.11 vs 0.75±0.06、0.46±0.06 vs 0.33±0.06(P<0.05),8 h后分别为0.33±0.03 vs 0.35±0.05(P>0.05);输注红细胞2、4、8 h,小鼠肝M1基因中诱导型一氧化氮合酶(iNOS)基因表达水平实验组和对照组分别为3.44±0.20 vs 2.46±0.08、9.25±0.55 vs 2.67±0.12、2.80±0.08 vs 2.39±0.01,肿瘤坏死因子-α(TNF-α)分别为1.69±0.22 vs 1.13±0.03、1.44±0.24 vs 0.96±0.09、1.31±0.05 vs 0.96±0.06,单核细胞趋化蛋白1(MCP1)分别为4.96±0.08 vs 4.28±0.27、4.63±0.04 vs 2.07±0.09、2.28±0.19 vs 1.33±0.03(P<0.05);M2基因中精氨酸1(Arg1)基因表达水平实验组和对照组分别为0.81±0.21 vs 0.82±0.18、0.66±0.11 vs 0.58±0.09、0.39±0.17 vs 0.37±0.15,甘露糖受体C型2(Mrc2)分别为0.99±0.91 vs 0.97±0.08、0.98±0.12 vs 1.02±0.11、0.59±0.19 vs 0.57±0.08,重组蛋白163(CD163)分别为1.75±0.20 vs 1.69±0.18、0.22±0.02 vs 0.21±0.01、0.04±0.01 vs 0.03±0.01(P>0.05)。结论实验小鼠输注贮存红细胞明显促进其肝脏组织巨噬细胞朝向M1表型的极化。
文摘目的考察滤除白细胞对全血保存质量和功能的影响。方法 1袋(2 U=400 mL)新鲜全血均分为滤除白细胞全血(简称滤白)组:按照去白滤器的使用说明书操作,在采血<6 h过滤制备去白细胞全血;对照组:不滤除白细胞。过滤及未过滤的全血均采用无菌方式分别分为6小袋[(30～40)mL/袋],(4±2)℃常规保存,分别于保存1、7、14、21、28、35 d各取1小袋,检测血常规、pH、游离血红蛋白(FHb)、氧亲和力(P50)、ATP、2,3-二磷酸甘油酸(2,3-DPG)、电解质、红细胞膜表面PS、CD47表达、葡萄糖、乳酸等指标,比较过滤与未过滤全血质量的差异。结果实验组1 U全血过滤后剩余白细胞数为(0.32±0.05)×106个;滤白组和对照组比较,保存35 d时血红蛋白(Hb)(g/L)为149.00±11.53 vs 137.20±3.96、MCV(fL)为98.90±3.23 vs 98.96±2.10、pH为6.78±0.02 vs6.75±0.02、P50(mmHg)为15.36±0.63 vs 15.18±0.22、ATP(μmol/gHb)为3.57±0.15 vs 3.61±1.02、2,3-DPG(mmol/L)为0.13±0.09 vs 0.22±0.2、PS阳性率(%)为0.96±0.08 vs 1.19±0.03、CD47阳性率(%)为47.65±3.92 vs 48.37±4.35(P>0.05),溶血率(%)为0.08±0.02 vs 0.12±0.03(P<0.05);保存28 d时乳酸(mmol/L)为15.76±0.19 vs 18.73±0.97、葡萄糖(mmol/L)为20.21±0.55 vs 18.57±0.46(P<0.05)。结论全血离体保存期间会发生一系列质量和功能的变化,滤除白细胞全血的溶血率、乳酸、葡萄糖等指标略优于未过滤的全血,二者的携释氧功能及其他生理生化指标均无明显差异,说明滤除白细胞未对全血保存期间的质量产生不良影响,并可能有利于血液保存。
