Background: Bloodstream infection is a serious infectious disease. In recent years, the drug resistance of pathogenic bacteria to commonly used anti-infective drugs has been widely concerned, which also makes the trea...Background: Bloodstream infection is a serious infectious disease. In recent years, the drug resistance of pathogenic bacteria to commonly used anti-infective drugs has been widely concerned, which also makes the treatment of bloodstream infection face severe challenges. Objective: To explore the distribution characteristics of blood culture-positive pathogens and the resistance to antibacterial drugs, so as to provide clinicians with accurate laboratory evidence, so as to guide clinicians to rationally apply antibiotics, improve clinical treatment effects, and reduce the emergence of drug-resistant strains. Methods: From January 2019 to June 2022, 2287 positive blood culture specimens of patients in Guangzhou Women and Children’s Medical Center were retrospectively analyzed, and the proportion of different pathogenic bacteria, the distribution of pathogenic bacteria in different departments, and the multi-drug resistance of different pathogenic bacteria were counted. Results: Among the 2287 blood culture positive samples, 1560 strains (68.20%) of gram-positive bacteria and 727 strains (31.80%) of gram-negative bacteria were strained. The top three departments in the distribution of pathogenic bacteria were pediatric intensive care unit (600 strains), pediatric internal medicine (514 strains), and pediatric emergency comprehensive ward (400 strains). The pathogens with high detection rates were: Staphylococcus epidermidis (24.09%), Staphylococcus humans (23.74%), Escherichia coli (13.21%) and Klebsiella pneumoniae (8.71%). The pathogens with high multi-drug resistance rates were: Streptococcus pneumoniae (93%), Staphylococcus epidermidis (83.76%), Enterobacter cloacae (75.61%) and Staphylococcus humans (62.43%). Conclusion: In our hospital, gram-positive bacteria were the main pathogenic bacteria in the blood culture of children patients. The children’s intensive care unit was the department with the largest distribution of pathogenic bacteria, and the multiple drug resistance rate of Streptococcus pneumoniae was the highest.展开更多
Background:Sepsis is a potentially life-threatening condition that occurs when the body’s response to infection leads to widespread inflammation and tissue damage.Negative cultures can make it difficult for clinician...Background:Sepsis is a potentially life-threatening condition that occurs when the body’s response to infection leads to widespread inflammation and tissue damage.Negative cultures can make it difficult for clinicians to make a diagnosis and may raise questions about the validity of the definition of sepsis.In addition,the clinical distinctions between burn patients with blood culturepositive and-negative sepsis are also poorly understood.Therefore,this study aimed to examine the clinical differences between blood culture-positive and-negative sepsis in burn patients in order to improve the understanding of the pathophysiology and epidemiology of sepsis in this population.Methods:This study had a retrospective design,and the participants were adults aged≥18 years.Patients diagnosed with sepsis were divided into two groups based on their blood culture results within 1 week of sepsis diagnosis.Results:We enrolled 1643 patients admitted to our institution’s burn intensive care unit between January 2010 and December 2021.pH,platelet count,bicarbonate and haematocrit were significant in both the positive and negative groups.However,lymphocyte,red cell distribution width and blood urea nitrogen were significant only in the positive group,whereas lactate dehydrogenase was significant only in the negative group.Acinetobacter baumannii,Pseudomonas aeruginosa,and Klebsiella pneumonia are common gram-negative bacterial species,and Staphylococcus aureus and Staphylococcus epidermidis are common gram-positive bacterial species seen in burn patients with positive blood cultures.Carbapenem resistance was found to be associated with an unfavourable prognosis in gram-negative bacteria,with the exception of P.aeruginosa.Conclusions:pH,platelet count,bicarbonate and haematocrit were routine biomarkers that demon-strated statistical significance in both groups.Lactate dehydrogenase was significant in the blood-negative group,while red cell distribution width,blood urea nitrogen and lymphocyte count were significant in the positive group.Furthermore,the most common causes of sepsis are gram-negative bacteria,including A.baumannii,K.pneumoniae and P.aeruginosa.Additionally,resistance to carbapenems is associated with unfavourable outcomes.展开更多
The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated...The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated not only microbial diversity but also drug resistant microbes largely habitat in ICU.Pseudomonas aeruginosa found as a part of normal intestinal flora and a significant pathogen responsible for wide range of ICU acquired infection in critically ill patients.Nosocomial infection associated with this organism including gastrointestinal infection,urinary tract infections and blood stream infection.Infection caused by this organism are difficult to treat because of the presence of its innate resistance to many antibiotics(b-lactam and penem group of antibiotics),and its ability to acquire further resistance mechanism to multiple class of antibiotics,including Beta-lactams,aminoglycosides and fluoroquinolones.In the molecular evolution microbes adopted several mechanism to maintain genomic plasticity.The tool microbe use for its survival is mainly biofilm formation,quorum sensing,and horizontal gene transfer and enzyme promiscuity.Such genomic plasticity provide an ideal habitat to grow and survive in hearse environment mainly antibiotics pressure.This review focus on infection caused by Pseudomonas aeruginosa,its mechanisms of resistance and available treatment options.The present study provides a systemic review on major source of Pseudomonas aeruginosa in ICU.Further,study also emphasizes virulence gene/s associated with Pseudomonas aeruginosa genome for extended drug resistance.Study gives detailed overview of antibiotic drug resistance mechanism.展开更多
文摘Background: Bloodstream infection is a serious infectious disease. In recent years, the drug resistance of pathogenic bacteria to commonly used anti-infective drugs has been widely concerned, which also makes the treatment of bloodstream infection face severe challenges. Objective: To explore the distribution characteristics of blood culture-positive pathogens and the resistance to antibacterial drugs, so as to provide clinicians with accurate laboratory evidence, so as to guide clinicians to rationally apply antibiotics, improve clinical treatment effects, and reduce the emergence of drug-resistant strains. Methods: From January 2019 to June 2022, 2287 positive blood culture specimens of patients in Guangzhou Women and Children’s Medical Center were retrospectively analyzed, and the proportion of different pathogenic bacteria, the distribution of pathogenic bacteria in different departments, and the multi-drug resistance of different pathogenic bacteria were counted. Results: Among the 2287 blood culture positive samples, 1560 strains (68.20%) of gram-positive bacteria and 727 strains (31.80%) of gram-negative bacteria were strained. The top three departments in the distribution of pathogenic bacteria were pediatric intensive care unit (600 strains), pediatric internal medicine (514 strains), and pediatric emergency comprehensive ward (400 strains). The pathogens with high detection rates were: Staphylococcus epidermidis (24.09%), Staphylococcus humans (23.74%), Escherichia coli (13.21%) and Klebsiella pneumoniae (8.71%). The pathogens with high multi-drug resistance rates were: Streptococcus pneumoniae (93%), Staphylococcus epidermidis (83.76%), Enterobacter cloacae (75.61%) and Staphylococcus humans (62.43%). Conclusion: In our hospital, gram-positive bacteria were the main pathogenic bacteria in the blood culture of children patients. The children’s intensive care unit was the department with the largest distribution of pathogenic bacteria, and the multiple drug resistance rate of Streptococcus pneumoniae was the highest.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(Grant No.NRF-2021R1A2C2006033).
文摘Background:Sepsis is a potentially life-threatening condition that occurs when the body’s response to infection leads to widespread inflammation and tissue damage.Negative cultures can make it difficult for clinicians to make a diagnosis and may raise questions about the validity of the definition of sepsis.In addition,the clinical distinctions between burn patients with blood culturepositive and-negative sepsis are also poorly understood.Therefore,this study aimed to examine the clinical differences between blood culture-positive and-negative sepsis in burn patients in order to improve the understanding of the pathophysiology and epidemiology of sepsis in this population.Methods:This study had a retrospective design,and the participants were adults aged≥18 years.Patients diagnosed with sepsis were divided into two groups based on their blood culture results within 1 week of sepsis diagnosis.Results:We enrolled 1643 patients admitted to our institution’s burn intensive care unit between January 2010 and December 2021.pH,platelet count,bicarbonate and haematocrit were significant in both the positive and negative groups.However,lymphocyte,red cell distribution width and blood urea nitrogen were significant only in the positive group,whereas lactate dehydrogenase was significant only in the negative group.Acinetobacter baumannii,Pseudomonas aeruginosa,and Klebsiella pneumonia are common gram-negative bacterial species,and Staphylococcus aureus and Staphylococcus epidermidis are common gram-positive bacterial species seen in burn patients with positive blood cultures.Carbapenem resistance was found to be associated with an unfavourable prognosis in gram-negative bacteria,with the exception of P.aeruginosa.Conclusions:pH,platelet count,bicarbonate and haematocrit were routine biomarkers that demon-strated statistical significance in both groups.Lactate dehydrogenase was significant in the blood-negative group,while red cell distribution width,blood urea nitrogen and lymphocyte count were significant in the positive group.Furthermore,the most common causes of sepsis are gram-negative bacteria,including A.baumannii,K.pneumoniae and P.aeruginosa.Additionally,resistance to carbapenems is associated with unfavourable outcomes.
文摘The emergence of antibiotic resistant bacteria in the healthcare is a serious concern.In the Healthcare premises precisely intensive care unit are major sources of microbial diversity.Recent findings have demonstrated not only microbial diversity but also drug resistant microbes largely habitat in ICU.Pseudomonas aeruginosa found as a part of normal intestinal flora and a significant pathogen responsible for wide range of ICU acquired infection in critically ill patients.Nosocomial infection associated with this organism including gastrointestinal infection,urinary tract infections and blood stream infection.Infection caused by this organism are difficult to treat because of the presence of its innate resistance to many antibiotics(b-lactam and penem group of antibiotics),and its ability to acquire further resistance mechanism to multiple class of antibiotics,including Beta-lactams,aminoglycosides and fluoroquinolones.In the molecular evolution microbes adopted several mechanism to maintain genomic plasticity.The tool microbe use for its survival is mainly biofilm formation,quorum sensing,and horizontal gene transfer and enzyme promiscuity.Such genomic plasticity provide an ideal habitat to grow and survive in hearse environment mainly antibiotics pressure.This review focus on infection caused by Pseudomonas aeruginosa,its mechanisms of resistance and available treatment options.The present study provides a systemic review on major source of Pseudomonas aeruginosa in ICU.Further,study also emphasizes virulence gene/s associated with Pseudomonas aeruginosa genome for extended drug resistance.Study gives detailed overview of antibiotic drug resistance mechanism.
