Objective: To study the regulation of blood pulse volume via photoplethysmography (PPG) signal detected from toe, while the lower limb is passively raised in different height positions. Methods: Use a modified non-inv...Objective: To study the regulation of blood pulse volume via photoplethysmography (PPG) signal detected from toe, while the lower limb is passively raised in different height positions. Methods: Use a modified non-invasive PPG technique to detect the blood pulse signal on toe with infrared (IR) photo sensor. A protocol consisting of two postures, i.e., supine and 45° reclining, was designed to conduct laboratory trial in this study. During the period of performing the protocol of these postures, the lower limb was passively raised from the heights of 10 cm to 60 cm randomly and individually with sponge blocks underneath the foot. Results: In the supine posture, the higher the foot was passively raised, the more the blood PPG signal decreased. In the 45° reclining posture, the blood PPG signal increased at the beginning and then decreased in the foot height position from 10 cm to 60 cm. In both postures the normalized AC signal changes significantly while the normalized DC signal changes little. Conclusion: The toe PPG signals can obviously indicate the regulated blood volume change with the designated postural procedures due to the heart level position.展开更多
Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly understood.Methods We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical...Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly understood.Methods We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical data of primary hypertensive patients admitted to our hospital.Secondly,we explored a rat model to study the effect of Hcy on blood pressure and the role of H2S.An hyperhomocysteinemia(HHcy)rat model was induced to explore the effect of Hcy on blood pressure and the possible mechanism.We carried out tissue histology,extraction and examination of RNA and protein.Finally,we conducted cell experiments to determine a likely mechanism through renin-angiotensin-aldosterone system(RAAS)and extracellular signal-regulated kinase 1/2(ERK1/2)signaling pathway.Results In primary hypertensive inpatients with HHcy,blood pressure was significantly higher as compared with inpatient counterparts lacking HHcy.In the rat model,blood pressure of the Wistar rats was significantly increased with increases in serum Hcy levels and decreased after folate treatment.Angiotensin converting enzyme 1(ACE1)expression in the Wistar Hcy group was enhanced comparing to controls,but was decreased in the Wistar folate group.Angiotensin II receptor type 1(AGTR1)levels in the kidney tissue increased in the Wistar folate group.Both serum H2S and kidney cystathionineγ-lyase decreased with elevated levels of serum Hcy.In vitro,increased concentrations and treatment times for Hcy were associated with increased expression of collagen type 1 and AGTR1.This dose and time dependent response was also observed for p-STAT3 and p-ERK1/2 expression.Conclusion Endogenous H2S might mediate the process of altered blood pressure in response to changes in serum Hcy levels,in a process that is partly dependent on activated RAAS and ERK1/2-STAT3 signaling pathway.展开更多
Objective To study the molecular biology mechanism of Danggui-buxue-tang on tonifying Qi and controlling blood circulation through modulation the immune functional genes.Methods Rats were randomly divided into control...Objective To study the molecular biology mechanism of Danggui-buxue-tang on tonifying Qi and controlling blood circulation through modulation the immune functional genes.Methods Rats were randomly divided into control group,model group,and Danggui-buxue-tang group.Effect of Dang-guibuxue-tang on mRNA expressions of IL-1β,TNF-α,HSP-70,NF-κB,p38MAPK and JNK in blood cells of rats with Qi deficiency and blood stasis were measured with real-time fluorescent quantitative-PCR at 5th,14th and 28th day.And that in artery wall were determined at the 28th day.NF-κB/p65 and p-c-jun protein expressions in rat artery wall were detected by western blotting as well.Results In model group,TNF-α,IL-1β,NF-κB,HSP-70,p38MAPK and JNK mRNA expression in blood cell increased significantly compared with control group.Compared with model group,mRNA expression of IL-1βand JNK decreased significantly;TNF-α decreased at 5th,14th day;HSP-70,p38MAPK decreased at 14th,28th day;NF-κB decreased at 28th day.In model group,TNF-α,IL-1β,NF-κB,p38MAPK and JNK mRNA expression in artery increased compared with control group,excluded HSP-70,and that in Danggui-buxue-tang group decreased significantly.In model group,NF-κB/p65 and p-c-jun protein expression in artery increased compared with control group,and that in Danggui-buxue-tang group decreased significantly.Conclusions The effects of Dang-guibuxue-tang on Qi deficiency and blood stasis syndrome was brought from the regulating of cytokine network at multi-link and multi-target,NF-κB,p38MAPK and JNK signal transduction pathways included.Through which the immune system and whole body reached to a functional balance status.展开更多
In order to analyze the experimental cardiovascular signal with high accuracy, a system, integrating real-time monitoring and off-line further analysis, was developed and verified. The design, data processing and anal...In order to analyze the experimental cardiovascular signal with high accuracy, a system, integrating real-time monitoring and off-line further analysis, was developed and verified. The design, data processing and analysis methods as well as testing results are described. With 5 sampling frequency choices and 8 channel data acquisition, the system achieved high performances in beat-to-beat monitoring, signal processing and analysis. Tests were carried out to validate its performance in real-time monitoring, effectiveness of digital filters, QRS and blood pressure detection reliability, and RR-interval timing accuracy. The QRS detection rate was at least 99.46% for the records with few noises from MIT-BIH arrhythmia database using the algorithm for real-time monitoring, and no less than 96.43% for the records with some noises. In the condition that noise amplitude levels were less than 80%,the standard deviations for RR-interval timing were less than 1 ms with a generated ECG corrupted with various noises from MIT-BIH Noise Stress Test Database. Besides, the system is open for function expansion to meet further study-specific needs.展开更多
目的:分析彩色多普勒超声在双波长激光治疗婴幼儿血管瘤的应用价值。方法:回顾性分析2021年3月至2023年3月收治的血管瘤患者临床资料,所有患者在行Cynergy双波长激光治疗前均接受彩色多普勒超声检查,根据不同的血流信号和皮损深度,观察...目的:分析彩色多普勒超声在双波长激光治疗婴幼儿血管瘤的应用价值。方法:回顾性分析2021年3月至2023年3月收治的血管瘤患者临床资料,所有患者在行Cynergy双波长激光治疗前均接受彩色多普勒超声检查,根据不同的血流信号和皮损深度,观察治疗效果及并发症发生情况。结果:198例血管瘤患儿纳入分析,共计212处皮损,其中根据血流信号分为无血流信号73例(34.3%)、稀少血流信号76例(35.8%)、中等血流信号63例(29.9%),有效率为100%,96.1%,85.7%,三组比较差异有统计学意义(P<0.05);根据皮损深度分为<1.2 mm 69例(32.5%),1.2~3.0 mm 88例(41.5%),>3 mm 55例(26.0%),有效率为100%,94.3%,83.6%,三组比较差异有统计学意义(P<0.05);在所有纳入的患者中,有21.7%的患儿出现不良反应。结论:彩色多普勒超声在双波长激光治疗婴幼儿血管瘤中具有重要的应用价值,可以实时监测治疗过程,并帮助临床医师预估疗效。展开更多
文摘Objective: To study the regulation of blood pulse volume via photoplethysmography (PPG) signal detected from toe, while the lower limb is passively raised in different height positions. Methods: Use a modified non-invasive PPG technique to detect the blood pulse signal on toe with infrared (IR) photo sensor. A protocol consisting of two postures, i.e., supine and 45° reclining, was designed to conduct laboratory trial in this study. During the period of performing the protocol of these postures, the lower limb was passively raised from the heights of 10 cm to 60 cm randomly and individually with sponge blocks underneath the foot. Results: In the supine posture, the higher the foot was passively raised, the more the blood PPG signal decreased. In the 45° reclining posture, the blood PPG signal increased at the beginning and then decreased in the foot height position from 10 cm to 60 cm. In both postures the normalized AC signal changes significantly while the normalized DC signal changes little. Conclusion: The toe PPG signals can obviously indicate the regulated blood volume change with the designated postural procedures due to the heart level position.
基金supported by the Beijing Natural Science Foundation Program(Grant number:5102040)the Open Foundation of the Beijing Key Laboratory of Hypertension Research(Grant number:2015GXYB01)
文摘Background Homocysteine(Hcy)is a risk factor for hypertension,although the mechanisms are poorly understood.Methods We first explored the relationship between Hcy levels and blood pressure(BP)by analyzing the clinical data of primary hypertensive patients admitted to our hospital.Secondly,we explored a rat model to study the effect of Hcy on blood pressure and the role of H2S.An hyperhomocysteinemia(HHcy)rat model was induced to explore the effect of Hcy on blood pressure and the possible mechanism.We carried out tissue histology,extraction and examination of RNA and protein.Finally,we conducted cell experiments to determine a likely mechanism through renin-angiotensin-aldosterone system(RAAS)and extracellular signal-regulated kinase 1/2(ERK1/2)signaling pathway.Results In primary hypertensive inpatients with HHcy,blood pressure was significantly higher as compared with inpatient counterparts lacking HHcy.In the rat model,blood pressure of the Wistar rats was significantly increased with increases in serum Hcy levels and decreased after folate treatment.Angiotensin converting enzyme 1(ACE1)expression in the Wistar Hcy group was enhanced comparing to controls,but was decreased in the Wistar folate group.Angiotensin II receptor type 1(AGTR1)levels in the kidney tissue increased in the Wistar folate group.Both serum H2S and kidney cystathionineγ-lyase decreased with elevated levels of serum Hcy.In vitro,increased concentrations and treatment times for Hcy were associated with increased expression of collagen type 1 and AGTR1.This dose and time dependent response was also observed for p-STAT3 and p-ERK1/2 expression.Conclusion Endogenous H2S might mediate the process of altered blood pressure in response to changes in serum Hcy levels,in a process that is partly dependent on activated RAAS and ERK1/2-STAT3 signaling pathway.
文摘Objective To study the molecular biology mechanism of Danggui-buxue-tang on tonifying Qi and controlling blood circulation through modulation the immune functional genes.Methods Rats were randomly divided into control group,model group,and Danggui-buxue-tang group.Effect of Dang-guibuxue-tang on mRNA expressions of IL-1β,TNF-α,HSP-70,NF-κB,p38MAPK and JNK in blood cells of rats with Qi deficiency and blood stasis were measured with real-time fluorescent quantitative-PCR at 5th,14th and 28th day.And that in artery wall were determined at the 28th day.NF-κB/p65 and p-c-jun protein expressions in rat artery wall were detected by western blotting as well.Results In model group,TNF-α,IL-1β,NF-κB,HSP-70,p38MAPK and JNK mRNA expression in blood cell increased significantly compared with control group.Compared with model group,mRNA expression of IL-1βand JNK decreased significantly;TNF-α decreased at 5th,14th day;HSP-70,p38MAPK decreased at 14th,28th day;NF-κB decreased at 28th day.In model group,TNF-α,IL-1β,NF-κB,p38MAPK and JNK mRNA expression in artery increased compared with control group,excluded HSP-70,and that in Danggui-buxue-tang group decreased significantly.In model group,NF-κB/p65 and p-c-jun protein expression in artery increased compared with control group,and that in Danggui-buxue-tang group decreased significantly.Conclusions The effects of Dang-guibuxue-tang on Qi deficiency and blood stasis syndrome was brought from the regulating of cytokine network at multi-link and multi-target,NF-κB,p38MAPK and JNK signal transduction pathways included.Through which the immune system and whole body reached to a functional balance status.
基金This work is supported by Beijing Natural Science Foundation (3052015)
文摘In order to analyze the experimental cardiovascular signal with high accuracy, a system, integrating real-time monitoring and off-line further analysis, was developed and verified. The design, data processing and analysis methods as well as testing results are described. With 5 sampling frequency choices and 8 channel data acquisition, the system achieved high performances in beat-to-beat monitoring, signal processing and analysis. Tests were carried out to validate its performance in real-time monitoring, effectiveness of digital filters, QRS and blood pressure detection reliability, and RR-interval timing accuracy. The QRS detection rate was at least 99.46% for the records with few noises from MIT-BIH arrhythmia database using the algorithm for real-time monitoring, and no less than 96.43% for the records with some noises. In the condition that noise amplitude levels were less than 80%,the standard deviations for RR-interval timing were less than 1 ms with a generated ECG corrupted with various noises from MIT-BIH Noise Stress Test Database. Besides, the system is open for function expansion to meet further study-specific needs.
文摘目的:分析彩色多普勒超声在双波长激光治疗婴幼儿血管瘤的应用价值。方法:回顾性分析2021年3月至2023年3月收治的血管瘤患者临床资料,所有患者在行Cynergy双波长激光治疗前均接受彩色多普勒超声检查,根据不同的血流信号和皮损深度,观察治疗效果及并发症发生情况。结果:198例血管瘤患儿纳入分析,共计212处皮损,其中根据血流信号分为无血流信号73例(34.3%)、稀少血流信号76例(35.8%)、中等血流信号63例(29.9%),有效率为100%,96.1%,85.7%,三组比较差异有统计学意义(P<0.05);根据皮损深度分为<1.2 mm 69例(32.5%),1.2~3.0 mm 88例(41.5%),>3 mm 55例(26.0%),有效率为100%,94.3%,83.6%,三组比较差异有统计学意义(P<0.05);在所有纳入的患者中,有21.7%的患儿出现不良反应。结论:彩色多普勒超声在双波长激光治疗婴幼儿血管瘤中具有重要的应用价值,可以实时监测治疗过程,并帮助临床医师预估疗效。
