Tumor-Specific Histo-Blood Group Antigens: Apropos of Two Cases

Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltransferases may suffer from posttranslation sequence modification. The author describes 2 autopsy cases, where in the background of the unusual metastatic tumor presentation, incompatible blood group antigenic determinants have been demonstrated using blood group specific lectins and monoclonal antibodies (mAb). In the first case, reported here, a 10-year-old girl developed an acute myeloid leukemia and died in a septic endotoxin shock after successful cytostatic treatment of a juvenile signet ring cell cancer of her colon. At autopsy there were no signs of tumor except bilateral apple-sized mucinous ovarian (Krukenberg) metastases. While she had erythrocyte phenotype of blood group A, the signet ring adenocarcinoma cells expressed blood group B incompatible antigenic determinants with lectin/mAb. In the second case, the autopsy of a 78-year-old female resulted in no macroscopic tumor sign except a moderately enlarged, ham hard spleen. Light microscopy revealed adenocarcinomatous infiltration in the splenic sinusoids. The patient had blood group O, while the metastatic cells in the spleen reacted with Breast Carcinoma Antigen (BioGenex) and incompatible anti-B Banderiaeasimplicifolia agglutinin I and anti-B mAb. It proved to be a case of an occult, completely regressed breast cancer. Based on these observations the expression of tumor specific incompatible blood group antigens might occur from time to time, mostly in adenocarcinomas. Accordingly, blood group-based specific immuno-oncotherapy could be considered in some cancer cases.

A Comparative Study Between Tumor Blood Vessels and Dynamic Contrast-enhanced MRI for Identifying Isocitrate Dehydrogenase Gene 1(IDH1)Mutation Status in Glioma

Objective Isocitrate dehydrogenase gene(IDH)mutations are associated with tumor angiogenesis and therefore play an important role in glioma management.This study compared the performance of tumor blood vessels counted from contrast-enhanced 3D brain volume(3D-BRAVO)sequence and dynamic contrast-enhanced(DCE)MRI in differentiating IDH1 status in gliomas.Methods Forty-four glioma patients[16 with IDH1 mutant-type(IDH1-MT),28 with IDH1 wild-type(IDH1-WT)]were retrospectively analyzed.A blood vessel entering a tumor was defined as an intratumoral vessel;a blood vessel adjacent to the edge of a tumor was defined as a peritumoral vessel.Combined vessels were defined as the sum of the intratumoral and peritumoral vessels.DCE-derived metrics of tumor were normalized to the contralateral normal-appearing white matter.Results Intratumoral,peritumoral,and combined tumor blood vessels were all significantly different between IDH1-MT and IDH1-WT gliomas,and the range of area under curves(AUCs)was 0.816–0.855.For DCE-derived parameters,cerebral blood volume,cerebral blood flow,mean transit time,and volume transfer constant were significantly different between IDH1-MT and IDH1-WT gliomas,and the range of AUCs was 0.703–0.756.Combined vessels possessed the best performance for identifying IDH1 mutations in gliomas(AUC:0.855,sensitivity:0.857,specificity:0.812,P<0.001).Conclusion The number of tumor blood vessels has comparable diagnostic performance with DCE-derived parameters for differentiating IDH1 mutations and can serve as a potential imaging biomarker to reflect IDH1 mutations in gliomas.

Numerical simulation of blood flow and interstitial fluid pressure in solid tumor microcirculation based on tumor-induced angiogenesis

A coupled intravascular-transvascular-interstitial fluid flow model is developed to study the distributions of blood flow and interstitial fluid pressure in solid tumor microcirculation based on a tumor-induced microvascular network. This is generated from a 2D nine-point discrete mathematical model of tumor angiogenesis and contains two parent vessels. Blood flow through the microvascular network and interstitial fluid flow in tumor tissues are performed by the extended Poiseuille＇s law and Darcy＇s law, respectively, transvascular flow is described by Starling＇s law; effects of the vascular permeability and the interstitial hydraulic conductivity are also considered. The simulation results predict the heterogeneous blood supply, interstitial hypertension and low convection on the inside of the tumor, which are consistent with physiological observed facts. These results may provide beneficial information for anti-angiogenesis treatment of tumor and further clinical research.

Application of Circulating Tumor Cells in Peripheral Blood in Judging the Prognosis of Patients with Renal Cancer and Related Indexes of Blood Coagulation

Objective: To investigate the value of the number of circulating tumor cells (CTC) in peripheral blood in the prognosis and coagulation-related indicators of patients with renal cancer. Methods: 65 patients with renal cell carcinoma (RCC) confirmed pathologically were divided into CTC positive group and CTC negative group according to the CTC count (5 pcs/3.5 ml). Compare the age, gender, tumor location, TNM (clinical stage), pathological grade, tissue type, lymph node metastasis, distant metastasis, prognosis and prothrombin time (PT), fibrinogen (FIB), partial coagulation of the two groups of patients The correlation between the results of zymogen time (APTT) and D-dimer (DD) and the number of CTC. Results: There were significant differences in TNM, lymph node metastasis, and distant metastasis between the two groups (P < 0.05). The number of CTC in patients was correlated with FIB and D-D levels (P < 0.05). Conclusion: The number of CTC in patients with renal cell carcinoma is correlated with some clinical phenotypes (TNM, lymph node metastasis, distant metastasis) and some coagulation indexes (FIB, D-D), and can jointly predict the prognosis of renal cancer.

Frankincense myrrh attenuates hepatocellular carcinoma by regulating tumor blood vessel development through multiple epidermal growth factor receptor-mediated signaling pathways

BACKGROUND In traditional Chinese medicine(TCM),frankincense and myrrh are the main components of the antitumor drug Xihuang Pill.These compounds show anticancer activity in other biological systems.However,whether frankincense and/or myrrh can inhibit the occurrence of hepatocellular carcinoma(HCC)is unknown,and the potential molecular mechanism(s)has not yet been determined.AIM To predict and determine latent anti-HCC therapeutic targets and molecular mechanisms of frankincense and myrrh in vivo.METHODS In the present study,which was based on the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(http://tcmspw.com/tcmsp.php),Universal Protein database(http://www.uniprot.org),GeneCards:The Human Gene Database(http://www.genecards.org/)and Comparative Toxicogenomics Database(http://www.ctdbase.org/),the efficacy of and mechanism by which frankincense and myrrh act as anti-HCC compounds were predicted.The core prediction targets were screened by molecular docking.In vivo,SMMC-7721 human liver cancer cells were transplanted as xenografts into nude mice to establish a subcutaneous tumor model,and two doses of frankincense plus myrrh or one dose of an EGFR inhibitor was administered to these mice continuously for 14 d.The tumors were collected and evaluated:the tumor volume and growth rate were gauged to evaluate tumor growth;hematoxylineosin staining was performed to estimate histopathological changes;immunofluorescence(IF)was performed to detect the expression of CD31,α-SMA and collagen IV;transmission electron microscopy(TEM)was conducted to observe the morphological structure of vascular cells;enzyme-linked immunosorbent assay(ELISA)was performed to measure the levels of secreted HIF-1αand TNF-α;reverse transcription-polymerase chain reaction(RT-qPCR)was performed to measure the mRNA expression of HIF-1α,TNF-α,VEGF and MMP-9;and Western blot(WB)was performed to determine the levels of proteins expressed in the EGFR-mediated PI3K/Akt and MAPK signaling pathways.RESULTS The results of the network pharmacology analysis showed that there were 35 active components in the frankincense and myrrh extracts targeting 151 key targets.The molecular docking analysis showed that both boswellic acid and stigmasterol showed strong affinity for the targets,with the greatest affinity for EGFR.Frankincense and myrrh treatment may play a role in the treatment of HCC by regulating hypoxia responses and vascular system-related pathological processes,such as cytokine-receptor binding,and pathways,such as those involving serine/threonine protein kinase complexes and MAPK,HIF-1 and ErbB signaling cascades.The animal experiment results were verified.First,we found that,through frankincense and/or myrrh treatment,the volume of subcutaneously transplanted HCC tumors was significantly reduced,and the pathological morphology was attenuated.Then,IF and TEM showed that frankincense and/or myrrh treatment reduced CD31 and collagen IV expression,increased the coverage of perivascular cells,tightened the connection between cells,and improved the shape of blood vessels.In addition,ELISA,RT-qPCR and WB analyses showed that frankincense and/or myrrh treatment inhibited the levels of hypoxia-inducible factors,inflammatory factors and angiogenesis-related factors,namely,HIF-1α,TNF-α,VEGF and MMP-9.Furthermore,mechanistic experiments illustrated that the effect of frankincense plus myrrh treatment was similar to that of an EGFR inhibitor with regard to controlling EGFR activation,thereby inhibiting the phosphorylation activity of its downstream targets:the PI3K/Akt and MAPK(ERK,p38 and JNK)pathways.CONCLUSION In summary,frankincense and myrrh treatment targets tumor blood vessels to exert anti-HCC effects via EGFR-activated PI3K/Akt and MAPK signaling pathways,highlighting the potential of this dual TCM compound as an anti-HCC candidate.

EFFECTS OF VARIOUS VASOACTIVE AGENTS ON TUMOR BLOOD FLOW IN RAT

Effects of angiotensin Ⅱ and other six vasoactive agents on tissue blood flow of Yoahida rat ascites hepatoma AH109A and normal liver were measured by the hydrogen clearance method. The mean blood flow in the tumor peripheral part under normal tension was 11. 9±8. 2ml/ min/100g tissue and was not influenced by tumor size. Tumor blood flow was more significantly increased in infusion angiotensin Ⅱthan 0.5mg/ ml methoxamine, however, normal liver blood flow of tumor-bearing rats was unchanged in contrast to an Increase seen in the tumor. A pronounced reduction of tumor blood flow was found after administration of epinephrine, norepinephrin and ethylphenylephrine. In addition, metaraminol and phenyleprine as well as 1. 0 and 2. 5mg/ ml methoxamine were not found to significantly change blood flow of the tumor.

ENHANCEMENT OF TUMOR GROWTH AFTER PARTIAL HEPATECTOMY AND BLOOD TRANSFUSION

Female adult BUF rats (6-8 weeks) received Sham operation (Sham); 70% hepatectomy (PH); Sham or PH with blood transfusion (BT or PH+BT). BUF 7316A hepatoma cells were inoculated subcutaneously in the neck of rats on the operation day. Tumor size was measured from day 7 to 20 after inoculation. Sera and splenic adherent cell harvested on day 5 from Sham and PH rat were added into Mixed Lymphocyte Cultures (MLR). The result showed that tumor growth in PH or BT rats was significantly promoted as compared to that in Sham rats (P<0.01,P<0.05). The most marked enhancement of tumor growth was observed in PH+BT rats (P<0.001). Sera and splenic adherent cell from PH rat significantly inhibited MLR (P<0.05). Those results suggest that partial hepatectomy and blood transfusion are responsible for the enhancement of tumor growth. Some immunosuppressive factor might be produced in the process of liver regeneration, and blood transfusion might have an additive immunosuppressive effect.

Clinical Value of Peripheral Blood Circulating Tumor Cells and Cell-Free DNA Combined Detection in Triple-Negative Breast Cancer

Objective:To determine the clinical value of combined detection of circulating tumor cells(CTCs)and cell-free DNA(cfDNA)in peripheral blood of patients with triple-negative breast cancer.Method:41 patients with breast cancer admitted to the First Central Hospital of Baoding from January 2020 to December 2021 were selected and recruited into the experimental group,42 patients with benign breast cancer admitted during the same period were recruited into the conditional control group,and 41 healthy patients admitted during the same period were recruited into the blank control group.The positive rate of peripheral blood CTCs,the level of cfDNA,and the diagnostic efficacy of peripheral blood CTCs,cfDNA alone and the combination thereof for breast cancer were analyzed.Result:The positive rates of peripheral blood CTCs in the experimental group,the conditional control group,and the blank control group were 43.90%,11.90%,and 9.74%,respectively,and there was significant difference among the groups.The levels of cfDNA in peripheral blood of the experimental group,the conditional control group,and the blank control group were 0.26±0.08 bp,0.17±0.03 bp,and 0.15±0.04 bp,respectively,which were statistically significant.The detection levels of 100 bp hTERT/ng mT1 and 241 bp hTERT/ng-mT1 in the experimental group were significantly higher than those in the conditional control group and the blank control group.The accuracy of peripheral blood CTCs detection in the three groups was 66.21%,the accuracy of cfDA241 bp/100 hp hTERT detection was 80.41%,and the accuracy of combined detection of peripheral blood CTCs and cfDNA was 94.03%.Conclusion:The clinical application of peripheral blood CTCs combined with cfDNA level detection can increase detection accuracy,provide data support for clinicians,and improve the clinical diagnostic effect of triple-negative breast cancer.

Alteration of Sex and Non-Sex Hormones and Distribution Features of Blood ABO System Groups among the Women with Uterine Body Tumors

Objectives: The aim of the investigation was to study the hormonal status (sex hormones: estradiol (E2), progesterone (P), testosterone (T);non-sex gonadotropic hormones-luteinizing hormone (LH) and follicle-stimulating hormone (FSH)) of women with benign and malignant tumors of uterine body in the reproductive, menopause and postmenopause periods. Also the distribution features of the blood ABO system phenotype groups and their link to the development of uterine body tumors have been studied. Methods: The determination of hormones was made by the enzyme analysis method (ELAIZA), provided by the proper ELAIZA kits. For the study of blood ABO system antigens, internationally recognized immunoserology methods were used. Results: Investigations revealed the increased level of E2 and T on the background of the reduced P in the blood of the women with uterine tumors in the reproductive, menopause and post-menopause period. As for gonadotropic hormones, the decreased levels of LH and FSH have also been detected. From the ABO system phenotype groups A(II) group had the highest frequency between the women with malignant uterine tumor in the reproductive age. O (I) phenotype group was the most frequent in case of menopause and post-menopause women with uterine malignant tumors. Conclusions: Hormonal imbalance creates good conditions for the proliferation of uterine tissues and hence causes the development of benign and malignant uterine tumors. The imbalance of the sex steroid and gonadotropic hormones in the blood of post-menopause women indicates on the genotoxic mechanism of cancer development on the background of age-related changes. A(II) group had the highest frequency between the reproductive age women with uterine malignant tumor, while O (I) group was the most frequent in case of menopause and post-menopause patients.

Glioma stem cells and the occurrence of tumor blood vessels

Epithelial glioma is the most common brain cancer,accounting for 35.26%-60.69%of intracranial tumors with an average of 44.69%,and it remains the greatest challenge in the field of neurosurgery.The median survival time of patients with advanced glioma is only 12 to 18 months due to the characteristics of high aggression,and the therapeutic effect was poor though surgery,chemotherapy,and targeted drug therapy being treated.Because of the presence of heterogeneity and the differentiation disorder,only a small number of glioma cells are the source of tumor growth and metastasis,which are highly resistant to traditional treatments.They are deemed as the“seed”tumor cells as they could get rid of the effect of the treatment and reconstruct the organization of tumor.They are also termed as brain tumor stem cell(BTSC)or glioma stem cells(GSCs)since neural stem cells share similar features with them.Recent data reveal that they are directly related with invasion,angiogenesis,tolerance,chemotherapy,recurrence of glioma.Based on the research result by the team,the paper elaborates the characteristics of GSCs and the relationship with the tumor angiogenesis.

3D numerical study of tumor blood perfusion and oxygen transport during vascular normalization

The changes of blood perfusion and oxygen transport in tumors during tumor vascular normalization are studied with 3-dimensional mathematical modeling and numerical simulation. The models of tumor angiogenesis and vascular-disrupting are used to simulate ＂un-normalized＂ and ＂normalized＂ vasculatures. A new model combining tumor hemodynamics and oxygen transport is developed. In this model, the intravasculartransvascular-interstitial flow with red blood cell（RBC） delivery is tightly coupled, and the oxygen resource is produced by heterogeneous distribution of hematocrit from the flow simulation. The results show that both tumor blood perfusion and hematocrit in the vessels increase, and the hypoxia microenvironment in the tumor center is greatly improved during vascular normalization. The total oxygen content inside the tumor tissue increases by about 67%, 51%, and 95% for the three approaches of vascular normalization,respectively. The elevation of oxygen concentration in tumors can improve its metabolic environment, and consequently reduce malignancy of tumor cells. It can also enhance radiation and chemotherapeutics to tumors.

Resilience provides mediating effect of resilience between fear of progression and sleep quality in patients with hematological malignancies

BACKGROUND Hematological tumors are common malignant tumors,with high morbidity and mortality rates.Most patients with hematological malignancies develop sleep disorders that seriously affect their life and health because of acute onset of disease,rapid progression,high recurrence rates,complex treatment methods,and treatment costs.AIM To explore the mediating effect of resilience on fear of disease progression and sleep quality in patients with hematological malignancies.METHODS A cross-sectional analysis of 100 patients with hematological malignancies,treated in the First Affiliated Hospital of Jinzhou Medical University between August 2022 and August 2023,was conducted.Patients were assessed using a general data survey,a simplified scale for the fear of progression(FoP)of disease,a resilience scale,and the Pittsburgh Sleep Quality Index.Statistical analysis was conducted to determine the relationship between various patient characteristics and FoP,resilience,and sleep quality.Spearman’s correlation analysis was used to examine the correlations between mental resilience,FoP,and sleep quality.RESULTS The total FoP score mean value in patients with hematological malignancies was 38.09±5.16;the total resilience score mean value was 40.73±7.04;and the Pittsburgh Sleep Quality Index score mean value was 10.72±1.90.FoP,resilience,and sleep quality of the patients were associated with family per capita monthly income and patient education level(P<0.05).Spearman correlation analysis revealed that FoP was negatively correlated with resilience and sleep quality scores(r=-0.560,-0.537,P<0.01),respectively,and resilience was significantly associated with sleep quality scores(r=0.688,P<0.01).Mediation analysis showed that the mediating effect of resilience between FoP and sleep quality in patients with hematological malignancies was-0.100 and accounted for 50.51%of the total effect.This indicated that FoP directly and indirectly affected sleep quality through the mesomeric effect of resilience.CONCLUSION Resilience is an intermediary variable between FoP and sleep quality in patients with hematological malignancies.Medical staff should evaluate and follow-up FoP and resilience to implement measures to improve sleep quality.

血清脂质相关指标与乳腺癌关系的研究进展

脂代谢紊乱与乳腺癌的发生发展及预后密切相关。探索血清脂质相关指标、降脂药物对乳腺癌发病及预后的影响,有助于寻找乳腺癌新的生化标志物,发现新的治疗靶点并对其进行更精准的个体化治疗。本文通过查阅相关文献,就血清脂质相关指标与乳腺癌之间的关系做一综述,为深入研究乳腺癌的发病机制及乳腺癌的预防、诊断及治疗提供新的方向。

外周血未成熟粒细胞在疾病诊断和预后中的研究进展

粒细胞是人体重要的免疫细胞,具有趋化、黏附、吞噬和杀菌的功能。某些特殊情况下外周血未成熟粒细胞增多,意味着病理情况的出现。正常情况下人体外周血以分叶核粒细胞为主,有少量杆状核粒细胞。该文概括总结了在部分疾病中未成熟粒细胞出现的临床意义,如在外伤、肿瘤、感染、应激反应及怀孕等情况下外周血未成熟粒细胞计数及其百分比增加,让读者对未成熟粒细胞有更深的认识,更加客观地分析和治疗疾病。未成熟粒细胞计数及其百分比增加可能与疾病的诊断和预后相关。因此,正确看待外周血未成熟粒细胞对疾病的治疗有积极意义。

植物多糖的生物学功能及其在水产养殖中的应用

随着我国水产养殖业规模逐渐扩大,水生动物养殖中疾病频发、养殖水体污染等问题日趋严重。抗生素和传统疫苗在水产生物养殖中长期使用引发了水体污染、耐药菌株产生、抗生素残留等问题,制约了水产养殖业的健康发展。植物多糖在替代抗生素方面起到了重要作用。植物多糖存在于植物的叶、花、根、茎及果实中,是通过糖苷键连接各种单糖的高分子聚合物,具有安全、高效、低残留、无耐药性等优点。植物多糖具有多种生物学功能,如调节免疫、抗氧化、降血糖、抗肿瘤、改善肠道健康等,在动物生产中应用可以起到改善机体健康、提高免疫力及抗氧化能力、改善生产性能等作用。因此,文章主要综述了植物多糖的理化性质、生物学功能及其在水产生物养殖中应用的研究进展,为其在水产生物养殖中的进一步推广与应用提供参考。

温阳活血汤联合艾灸治疗寒凝血瘀型痛经临床研究

目的:探讨温阳活血汤联合艾灸治疗寒凝血瘀型痛经临床疗效。方法:选取原发性寒凝血瘀型痛经患者60例,将其随机分为两组,其中对照组单纯接受艾灸腰阳关治疗,观察组则接受艾灸腰阳关联合温阳活血汤治疗。观察两组治疗后临床疗效,同时进行痛经症状评分,在干预前、干预3个月经周期后采用VAS评分量表评估两组疼痛程度。另取血清以酶联免疫法检测白细胞介素-8(IL-8)、超敏C反应蛋白(hs-CRP)和肿瘤坏死因子-α(TNF-α)水平及前列腺素E2(PGE2)、神经生长因子(NGF)和前列腺素F2α(PGF2α)水平。结果:对照组和观察组总有效率分别为76.67%和93.33%,观察组明显高于对照组(P<0.05)。治疗后3个月,两组的痛经相关中医症状评分均显著低于治疗前,且观察组的症状评分均显著降低(均P<0.05);两组的疼痛视觉模拟评分(VAS)评分和COX痛经症状量表(CMSS)评分均降低,且与对照组相比,观察组的VAS评分和CMSS评分更低(均P<0.05)。治疗后,两组IL-8、hs-CRP、TNF-α、PGE2、NGF和PGF2α水平均较治疗前显著降低,与对照组比较,观察组水平降低更明显(均P<0.05)。观察组和对照组的不良反应发生率分别为6.67%和10.00%(P>0.05)。结论:温阳活血汤联合艾灸可降低IL-8、hs-CRP、TNF-α、PGE2、NGF和PGF2α水平,改善患者痛经症状。

虫类药在肿瘤高凝状态中的应用

肿瘤患者通常伴有血液高凝状态,易形成深静脉血栓、脑栓塞、肺栓塞等危及生命。现代医学抗凝治疗有出血风险,虫类药善攻逐走窜、破结逐瘀,能有效改善肿瘤高凝状态,现代药理学研究证实虫类药多具有抗凝血、溶解血栓、改善微循环的作用,可以通过抑制凝血酶活性、抑制血小板黏附、溶解纤维蛋白、改善血管内皮损伤等多种机制改善高凝状态,临床上各医家将其广泛运用于肿瘤高凝状态的治疗。

6例心内血性囊肿临床及病理特征分析

目的:分析心内血性囊肿的临床与病理特征,以提高对罕见心脏肿瘤的认识。方法:收集首都医科大学附属北京安贞医院2015年3月至2023年5月,诊断的6例心内血性囊肿病例,进行临床资料分析与病理形态学特征观察。结果:女性2例,男性4例;年龄25~59岁,中位年龄52.5岁。血性囊肿位于二尖瓣3例、主动脉瓣1例、三尖瓣1例、右心房1例。组织病理学表现为囊壁由纤维结缔组织构成,衬覆血管内皮细胞,腔内可见出血及血栓形成。免疫组织化学染色示CD31(+)、CD34(+)、D2-40(-)、Calretinin(-)。所有病例均完整切除,术后随访6例,均健在、无复发。结论:心内血性囊肿是极其少见的心脏良性肿瘤,术前通过超声心动图检查可高度提示此肿物可能,确诊需组织病理学检查。本病手术完整切除后通常预后良好。

基于破血逐瘀理论探讨中药水蛭及其复方治疗肿瘤的研究进展

肿瘤是临床中比较难治疗的疾病,化疗药物的耐药性以及治疗过程中的不良反应增加了治疗的难度。中药作为天然药物,具有不良反应少、遗传毒性低等优点,可以在治疗肿瘤疾病的同时对机体功能从根本上进行调理,通过查阅文献发现破血逐瘀药物,如三棱、莪术、水蛭、斑蝥等用于治疗肿瘤疗效确切,基于此,从破血逐瘀理论出发,分析肿瘤的中医病因病机及破血逐瘀治则,总结水蛭临床治疗肿瘤的部分作用机制,旨在为临床诊疗该疾病提供参考及研究方向。

基于心电心音图—血液指标的列线图对化疗后心脏不良事件风险的预测研究

目的探讨心电心音图联合血液指标预测恶性肿瘤患者化疗后发生心脏不良事件的风险。方法前瞻性收集171例恶性肿瘤患者。根据患者化疗结束后是否出现心脏不良事件分为心脏不良事件组和无心脏不良事件组,分析两组患者一般资料、化疗前血液指标与化疗早期(1~3周期)心电心音图相关指标,将可能影响因素进行二元Logistic回归分析确定独立预测因素并绘制列线图,利用受试者操作特征(ROC)曲线评估列线图预测能力。结果171例患者化疗结束后有44例发生心脏不良事件,发生率25.73%。二元logistic回归结果表示年龄、化疗前红细胞分布宽度(RDW)、活化部分凝血酶原时间(APTT)和化疗早期心脏电−机械激动时间(EMAT)是化疗患者发生心脏不良事件的独立预测因子,基于此绘制列线图,ROC曲线评估AUC为0.768(95%CI:0.693~0.843,P<0.001)。结论基于年龄、化疗前RDW、APTT和化疗早期EMAT绘制的列线图有助于早期评估化疗患者发生心脏不良事件的风险。