Previous studies show that chronic acrylamide exposure leads to central and peripheral neu- ropathy. However, the underlying mechanisms remained unclear. In this study, we examined the permeability of the blood-cerebr...Previous studies show that chronic acrylamide exposure leads to central and peripheral neu- ropathy. However, the underlying mechanisms remained unclear. In this study, we examined the permeability of the blood-cerebrospinal fluid barrier, and its ability to secrete transthyretin and transport leptin of rats exposed to acrylamide for 7, 14, 21 or 28 days. Transthyretin levels in cerebrospinal fluid began to decline on day 7 after acrylamide exposure. The sodium fluorescein level in cerebrospinal fluid was increased on day 14 after exposure. Evans blue concentration in cerebrospinal fluid was increased and the cerebrospinal fluid/serum leptin ratio was decreased on days 21 and 28 after exposure. In comparison, the cerebrospinal fluid/serum albumin ratio was increased on day 28 after exposure. Our findings show that acrylamide exposure damages the blood-cerebrospinal fluid barrier and impairs secretory and transport functions. These changes may underlie acrylamide-induced neurotoxicity.展开更多
Glaucoma has been recently characterized as a member of the group of anoikis-related diseases. Anoikis, a form of apoptosis, can be triggered by the unfastening of adherent junctions present in astrocytes. In those ar...Glaucoma has been recently characterized as a member of the group of anoikis-related diseases. Anoikis, a form of apoptosis, can be triggered by the unfastening of adherent junctions present in astrocytes. In those areas of the central nervous system in which the soma of the neurons or their axons and dendrites are metabolically dependent on the activity of astrocytes, a derangement of the lactate shuttle caused by a separation between the plasma membranes of neurons and astrocytes would result in metabolic impairment of the neurons themselves. In glaucoma, the triggering event has been attributed to the posterior deviation of aqueous humor towards the astrocyte-rich prelaminar tissue of the optic nerve head. The mean calcium content in the aqueous is able to interfere with calcium-dependent adherent junctions and induce anoikis of the astrocytes. As the cerebrospinal fluid has a similar base calcium concentration, a shunt of cerebrospinal fluid through the cerebral parenchyma would be able to interfere in the astrocytic architecture with dire consequences to the metabolically dependent neurons. Here the similitude between glaucoma,amyotrophic lateral sclerosis and Alzheimer's disease are discussed and the concept of the break in the fluid-brain barrier, as an event separated from the blood-brain barrier, is stressed.展开更多
Previous studies have shown that reduced sleep duration,sleep fragmentation,and decreased sleep quality in patients with Alzheimer's disease are related to dysfunction in orexin signaling.At the same time,blood-br...Previous studies have shown that reduced sleep duration,sleep fragmentation,and decreased sleep quality in patients with Alzheimer's disease are related to dysfunction in orexin signaling.At the same time,blood-brain barrier disruption is considered an early biomarker of Alzheimer's disease.However,currently no report has examined how changes in orexin signaling relate to changes in the blood-brain barrier of patients who have Alzheimer's disease with sleep insufficiency.This cross-sectional study included 50 patients with Alzheimer's disease who received treatment in 2019 at Beijing Tiantan Hospital.Patients were divided into two groups:those with insufficient sleep(sleep duration≤6 hours,n=19,age 61.58±8.54 years,10 men)and those with normal sleep durations(sleep duration>6 hours,n=31,age 63.19±10.09 years,18 men).Demographic variables were collected to evaluate cognitive function,neuropsychiatric symptoms,and activities of daily living.The levels of orexin,its receptor proteins,and several blood-brain barrier factors were measured in cerebrospinal fluid.Sleep insufficiency was associated with impaired overall cognitive function that spanned multiple cognitive domains.Furthermore,levels of orexin and its receptors were upregulated in the cerebrospinal fluid,and the blood–brain barrier was destroyed.Both these events precipitated each other and accelerated the progression of Alzheimer's disease.These findings describe the clinical characteristics and potential mechanism underlying Alzheimer's disease accompanied by sleep deprivation.Inhibiting the upregulation of elements within the orexin system or preventing the breakdown of the blood-brain barrier could thus be targets for treating Alzheimer's disease.展开更多
目的:分析脑干带状高信号的软脑膜转移病例的临床特征。方法:收集海南医学院第一附属医院及检索知网、万方、维普、PubMed、Web of Science等数据库中报道磁共振表现为脑干带状高信号的患者,收集并整理纳入患者的既往病史、症状、体征...目的:分析脑干带状高信号的软脑膜转移病例的临床特征。方法:收集海南医学院第一附属医院及检索知网、万方、维普、PubMed、Web of Science等数据库中报道磁共振表现为脑干带状高信号的患者,收集并整理纳入患者的既往病史、症状、体征、基因检查结果、脑脊液表现、治疗及预后等。结果:共纳入28例患者,其中26例有肺腺癌病史,2例发现肺部占位性改变,磁共振均表现为T2‐FLAIR上脑干腹侧呈带状高信号,两侧对称,可延伸至小脑脚,DWI呈高信号,ADC呈低信号,T1加权成像为长T1信号,T2加权成像为长T2信号,增强扫描未见强化。结论:识别肺癌继发软脑膜转移很重要,T2‐FLAIR和DWI上脑干无强化带状高信号很可能是非小细胞肺癌继发软脑膜转移的特征性表现。展开更多
α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and...α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and highly phospho rylated a-synuclein constitutes the main component of Lewy bodies in the brain,the pathological hallmark of Parkinson s disease.For decades,much attention has been focused on the accumulation of α-synuclein in the brain parenchyma rather than considering Parkinson s disease as a systemic disease.Recent evidence demonstrates that,at least in some patients,the initial α-synuclein pathology originates in the peripheral organs and spreads to the brain.Injection of α-synuclein preformed fibrils into the gastrointestinal tra ct trigge rs the gutto-brain propagation of α-synuclein pathology.However,whether α-synuclein pathology can occur spontaneously in peripheral organs independent of exogenous α-synuclein preformed fibrils or pathological α-synuclein leakage from the central nervous system remains under investigation.In this review,we aimed to summarize the role of peripheral α-synuclein pathology in the pathogenesis of Parkinson’s disease.We also discuss the pathways by which α-synuclein pathology spreads from the body to the brain.展开更多
Objective:The aim was to analyse the clinical features of leptomeningeal metastasis with banded high signal in the brainstem.Methods:In this paper,we report two cases of lung adenocarcinoma with soft meningeal metasta...Objective:The aim was to analyse the clinical features of leptomeningeal metastasis with banded high signal in the brainstem.Methods:In this paper,we report two cases of lung adenocarcinoma with soft meningeal metastasis,collected from the First Affiliated Hospital of Hainan Medical College,and searched the databases of CNKI,Wanfang,VIP,PubMed,Web of Science,and other databases which reported the MRI manifestation of"brainstem bandlike high signal",and collected the patients'past medical history,symptoms,signs,genetic findings,cerebrospinal fluid manifestation,treatment,and prognosis.Result:A total of 28 patients were included,of whom 26 had a history of lung adenocarcinoma and 2 were found to have occupational changes in the lungs.Magnetic resonance imaging(MRI)showed a band-like high signal in the ventral part of the brainstem on T2-FLAIR,symmetrical on both sides,which could extend to the cerebellar peduncles,with high signals on diffusion-weighted imaging(DWI),low signals on apparent diffusion coefficient(ADC),and long T1 signals on T1-weighted imaging,long T2 signals on T2 weighted imaging,and no long T2 signals on enhancement scan.T1-weighted imaging was a long T1 signal,T2-weighted imaging was a long T2 signal,and no enhancement was seen on enhanced scanning.Conclusion:It is important to recognize leptomeningeal metastasis of lung cancer,and the non-enhancing band of high signal in the brainstem on T2-FLAIR and DWI is likely to be the characteristic manifestation of leptomeningeal metastasis of non-small cell lung cancer.展开更多
The present study stems from the realization that the general problem relating to the analysis of wind-induced vibrations in suspension bridges still requires significant attention.Sidewalk railings,overhaul tracks,an...The present study stems from the realization that the general problem relating to the analysis of wind-induced vibrations in suspension bridges still requires significant attention.Sidewalk railings,overhaul tracks,and deflectors are known to largely affect such dynamics.Here,the influence of a row of water-filled traffic barriers on the response of a sample suspension bridge is investigated numerically.It is shown that the existence of water barriers causes flow separation and non-negligible vortices with respect to the condition with no water barriers.The vortex shedding frequency at the far end is around 41.30 Hz,relatively close to the real vibration frequency.It is also shown how different incoming angles of attack can change the flow field around the bridge cross-section and the vortex detachment frequency.展开更多
A numerical investigation is conducted to explore the evolution of a plasma discharge and its interaction with the fluid flow based on a self-consistent fluid model which couples the discharge dynamics with the fluid ...A numerical investigation is conducted to explore the evolution of a plasma discharge and its interaction with the fluid flow based on a self-consistent fluid model which couples the discharge dynamics with the fluid dynamics.The effects of the applied voltage on the distribution of velocity and temperature in initially static air are parainetrically studied.Furthermore,the spatial structure of plasma discharge and the resulting force contours in streamwise and normal directions are discussed in detail.The result shows that the plasma actuator produces a net force that should always be directed away from the exposed electrode,which results in an ionic wind pushing particles into a jet downstream of the actuator.When the energy added by the plasma is taken into account,the ambient air temperature is increased slightly around the electrode,but the velocity is almost not affected.Therefore it is unlikely that the induced flow is buoyancy driven.For the operating voltages considered in this paper,the maximum induced velocity is found to follow a power law,i.e.,it is proportional to the applied voltage to the 3.5 power.This promises an efficient application in the flow control with plasma actuators.展开更多
A three-porous-medium model for transvascular exchange and extravascular transport of fluid and macromolecules in a spherical solid tumor is developed. The microvasculature , lymphatics, and tissue space are each trea...A three-porous-medium model for transvascular exchange and extravascular transport of fluid and macromolecules in a spherical solid tumor is developed. The microvasculature , lymphatics, and tissue space are each treated as a porous medium with the flow of blood. lymph, and interstitial fluid obeying Darcy's law and Starling 's assumption. In this part, the role of interstitial pressure and fluid convection are studited. The analytical soiutions are obtained for foe isolated tumor and the normal-tissue-surrounded tumor respectively. The calculated interstitial pressure profue are consistent with the experimental observation that the elevated interstitial pressure is a major barrier in the penetration of macromolecular drug into tumors. The factors which may reduce the interstitial pressure are analyzed in details.展开更多
In vertebrates, early brain development takes place at the expanded anterior end of the neural tube, which is filled with embryonic cerebrospinal fluid (E-CSF). We have recently identified a transient blood–CSF barri...In vertebrates, early brain development takes place at the expanded anterior end of the neural tube, which is filled with embryonic cerebrospinal fluid (E-CSF). We have recently identified a transient blood–CSF barrier that forms between embryonic days E3 and E4 in chick embryos and that is responsible for the transport of proteins and control of E-CSF homeostasis, including osmolarity. Here we examined the presence of glucose transporter GLUT-1 as well the presence of caveolae-structural protein Caveolin1 (CAV-1) in the embryonic blood-CSF barrier which may be involved in the transport of glucose and of proteins, water and ions respectively across the neuroectoderm. In this paper we demonstrate the presence of GLUT-1 and CAV-1 in endothelial cells of blood vessels as well as in adjacent neuroectodermal cells, located in the embryonic blood–CSF barrier. In blood vessels, these proteins were detected as early as E4 in chick embryos and E12.7 in rat embryos, i.e. the point at which the embryonic blood–CSF barrier acquires this function. In the neuroectoderm of the embryonic blood-CSF barrier, GLUT-1 was also detected at E4 and E12.7 respectively, and CAV-1 was detected shortly thereafter in both experimental models. These experiments contribute to delineating the extent to which the blood–CSF embryonic barrier controls E-CSF composition and homeostasis during early stages of brain development in avians and mammals. Our results suggest the regulation of glucose transport to the E-CSF by means of GLUT-1 and also suggest a mechanism by which proteins are transported via transcellular routes across the neuroectoderm, thus reinforcing the crucial role of E-CSF in brain development.展开更多
Both experimental and simulated studies of microdischarge(MD)are carried out in a dielectric barrier discharge with a pin-to-pin gap of 3.5 mm,ignited by a sinusoidal voltage with a peak voltage of 10 kV and a driving...Both experimental and simulated studies of microdischarge(MD)are carried out in a dielectric barrier discharge with a pin-to-pin gap of 3.5 mm,ignited by a sinusoidal voltage with a peak voltage of 10 kV and a driving frequency of 5 kHz.Statistical results have shown that the probability of the single current pulse in the positive half-period(HP)reaches 73.6%under these conditions.Experimental results show that great luminous intensity is concentrated on the dielectric surface and the tip of the metal electrode.A 1D plasma fluid model is implemented by coupling the species continuity equations,electron energy density equations,Poisson equation,and Helmholtz equations to analyze the MD dynamics on the microscale.The simulated results are in good qualitative agreement with the experimental results.The simulated results show that the MD dynamics can be divided into three phases:the Townsend phase,the streamer propagation phase,and the discharge decay phase.During the streamer propagation phase,the electric field and electron density increase with the streamer propagation from the anode to the cathode,and their maximal values reach 625.48 Td and 2.31×10^(19)m^(-3),as well as 790.13 Td and 3.58×10^(19)m^(-3)in the positive and negative HP,respectively.Furthermore,a transient glow-like discharge is detected around the anode during the same period of streamer propagation.The formation of transient glow-like discharge is attributed to electrons drifting back to the anode,which is driven by the residual voltage in the air gap.展开更多
基金supported by State Key Development Program for Basic Research of China,No.2012CB525002the National Natural Science Foundation of China,No.30771823
文摘Previous studies show that chronic acrylamide exposure leads to central and peripheral neu- ropathy. However, the underlying mechanisms remained unclear. In this study, we examined the permeability of the blood-cerebrospinal fluid barrier, and its ability to secrete transthyretin and transport leptin of rats exposed to acrylamide for 7, 14, 21 or 28 days. Transthyretin levels in cerebrospinal fluid began to decline on day 7 after acrylamide exposure. The sodium fluorescein level in cerebrospinal fluid was increased on day 14 after exposure. Evans blue concentration in cerebrospinal fluid was increased and the cerebrospinal fluid/serum leptin ratio was decreased on days 21 and 28 after exposure. In comparison, the cerebrospinal fluid/serum albumin ratio was increased on day 28 after exposure. Our findings show that acrylamide exposure damages the blood-cerebrospinal fluid barrier and impairs secretory and transport functions. These changes may underlie acrylamide-induced neurotoxicity.
文摘Glaucoma has been recently characterized as a member of the group of anoikis-related diseases. Anoikis, a form of apoptosis, can be triggered by the unfastening of adherent junctions present in astrocytes. In those areas of the central nervous system in which the soma of the neurons or their axons and dendrites are metabolically dependent on the activity of astrocytes, a derangement of the lactate shuttle caused by a separation between the plasma membranes of neurons and astrocytes would result in metabolic impairment of the neurons themselves. In glaucoma, the triggering event has been attributed to the posterior deviation of aqueous humor towards the astrocyte-rich prelaminar tissue of the optic nerve head. The mean calcium content in the aqueous is able to interfere with calcium-dependent adherent junctions and induce anoikis of the astrocytes. As the cerebrospinal fluid has a similar base calcium concentration, a shunt of cerebrospinal fluid through the cerebral parenchyma would be able to interfere in the astrocytic architecture with dire consequences to the metabolically dependent neurons. Here the similitude between glaucoma,amyotrophic lateral sclerosis and Alzheimer's disease are discussed and the concept of the break in the fluid-brain barrier, as an event separated from the blood-brain barrier, is stressed.
基金supported by the National Key Research and Development Program of China,Nos.2016YFC1306300(to XMW),2016YFC1306000the National Key R&D Program of China-European Commission Horizon 2020,No.2017YFE0118800-779238(to YXW)+15 种基金the Notional Natural Science Foundation of Chino,Nos.81970992(to WZ),81571229(to WZ),81071015(to WZ),30770745(to WZ)Capital's Funds for Health Improvement and Research(CFH),No.2022-2-2048(to WZ)the Key Technology R&D Program of Beijing Municipal Education Commission,No.kz201610025030(to WZ)the Natural Science Foundation of Beijing,No.7082032(to WZ)the Key Project of the Natural Science Foundation of Beijing,No.4161004(to WZ)Capitol Clinical Characteristic Applicotion Research,No.Z121107001012161(to WZ)Project of Scientific and Technological Development of Traditional Chinese Medicine in Beijing,No.JJ2018-48(to WZ)High Level Technical Personnel Training Project of Beijing Health System of China,No.2009-3-26(to WZ)Excellent Personnel Training Project of Beijing,No.20071D0300400076(to WZ)Important National Science&Technology Specific Project,No.2011ZX09102-003-01(to WZ)Beijing Healthcare Research Project,No.JING-15-2(to WZ)Basic-Clinicol Research Cooperation Funding of Capitol Medical University of China,Nos.2015-JL-PT-X04(to WZ),10JL49(to WZ),14JL15(to WZ)the Natural Science Foundation of Capital Medical UniversityBeijingChina,No.PYZ2018077(to PG)Youth Research Fund of Beijing Tianton Hospital of Capital Medical University of China,Nos.2015-YQN-14(to PG),2015-YQN-15,2015-YQN-17。
文摘Previous studies have shown that reduced sleep duration,sleep fragmentation,and decreased sleep quality in patients with Alzheimer's disease are related to dysfunction in orexin signaling.At the same time,blood-brain barrier disruption is considered an early biomarker of Alzheimer's disease.However,currently no report has examined how changes in orexin signaling relate to changes in the blood-brain barrier of patients who have Alzheimer's disease with sleep insufficiency.This cross-sectional study included 50 patients with Alzheimer's disease who received treatment in 2019 at Beijing Tiantan Hospital.Patients were divided into two groups:those with insufficient sleep(sleep duration≤6 hours,n=19,age 61.58±8.54 years,10 men)and those with normal sleep durations(sleep duration>6 hours,n=31,age 63.19±10.09 years,18 men).Demographic variables were collected to evaluate cognitive function,neuropsychiatric symptoms,and activities of daily living.The levels of orexin,its receptor proteins,and several blood-brain barrier factors were measured in cerebrospinal fluid.Sleep insufficiency was associated with impaired overall cognitive function that spanned multiple cognitive domains.Furthermore,levels of orexin and its receptors were upregulated in the cerebrospinal fluid,and the blood–brain barrier was destroyed.Both these events precipitated each other and accelerated the progression of Alzheimer's disease.These findings describe the clinical characteristics and potential mechanism underlying Alzheimer's disease accompanied by sleep deprivation.Inhibiting the upregulation of elements within the orexin system or preventing the breakdown of the blood-brain barrier could thus be targets for treating Alzheimer's disease.
文摘目的:分析脑干带状高信号的软脑膜转移病例的临床特征。方法:收集海南医学院第一附属医院及检索知网、万方、维普、PubMed、Web of Science等数据库中报道磁共振表现为脑干带状高信号的患者,收集并整理纳入患者的既往病史、症状、体征、基因检查结果、脑脊液表现、治疗及预后等。结果:共纳入28例患者,其中26例有肺腺癌病史,2例发现肺部占位性改变,磁共振均表现为T2‐FLAIR上脑干腹侧呈带状高信号,两侧对称,可延伸至小脑脚,DWI呈高信号,ADC呈低信号,T1加权成像为长T1信号,T2加权成像为长T2信号,增强扫描未见强化。结论:识别肺癌继发软脑膜转移很重要,T2‐FLAIR和DWI上脑干无强化带状高信号很可能是非小细胞肺癌继发软脑膜转移的特征性表现。
基金supported by the National Natural Science Foundation of China,Nos.82271447,81771382the National Key Research and Development Program of China,No.2019 YFE0115900the"New 20 Terms of Universities in Jinan,No.202228022 (all to ZZ)。
文摘α-Synuclein is a protein that mainly exists in the presynaptic terminals.Abnormal folding and accumulation of α-synuclein are found in several neurodegenerative diseases,including Parkinson’s disease.Aggregated and highly phospho rylated a-synuclein constitutes the main component of Lewy bodies in the brain,the pathological hallmark of Parkinson s disease.For decades,much attention has been focused on the accumulation of α-synuclein in the brain parenchyma rather than considering Parkinson s disease as a systemic disease.Recent evidence demonstrates that,at least in some patients,the initial α-synuclein pathology originates in the peripheral organs and spreads to the brain.Injection of α-synuclein preformed fibrils into the gastrointestinal tra ct trigge rs the gutto-brain propagation of α-synuclein pathology.However,whether α-synuclein pathology can occur spontaneously in peripheral organs independent of exogenous α-synuclein preformed fibrils or pathological α-synuclein leakage from the central nervous system remains under investigation.In this review,we aimed to summarize the role of peripheral α-synuclein pathology in the pathogenesis of Parkinson’s disease.We also discuss the pathways by which α-synuclein pathology spreads from the body to the brain.
基金Hainan Clinical Medical Center Construction Project(2021)Excellent Talent Team of Hainan Province(No.QRCBT202121)Key R&D Program of Hainan Province(No.ZDYF2022SHFZ109)。
文摘Objective:The aim was to analyse the clinical features of leptomeningeal metastasis with banded high signal in the brainstem.Methods:In this paper,we report two cases of lung adenocarcinoma with soft meningeal metastasis,collected from the First Affiliated Hospital of Hainan Medical College,and searched the databases of CNKI,Wanfang,VIP,PubMed,Web of Science,and other databases which reported the MRI manifestation of"brainstem bandlike high signal",and collected the patients'past medical history,symptoms,signs,genetic findings,cerebrospinal fluid manifestation,treatment,and prognosis.Result:A total of 28 patients were included,of whom 26 had a history of lung adenocarcinoma and 2 were found to have occupational changes in the lungs.Magnetic resonance imaging(MRI)showed a band-like high signal in the ventral part of the brainstem on T2-FLAIR,symmetrical on both sides,which could extend to the cerebellar peduncles,with high signals on diffusion-weighted imaging(DWI),low signals on apparent diffusion coefficient(ADC),and long T1 signals on T1-weighted imaging,long T2 signals on T2 weighted imaging,and no long T2 signals on enhancement scan.T1-weighted imaging was a long T1 signal,T2-weighted imaging was a long T2 signal,and no enhancement was seen on enhanced scanning.Conclusion:It is important to recognize leptomeningeal metastasis of lung cancer,and the non-enhancing band of high signal in the brainstem on T2-FLAIR and DWI is likely to be the characteristic manifestation of leptomeningeal metastasis of non-small cell lung cancer.
文摘The present study stems from the realization that the general problem relating to the analysis of wind-induced vibrations in suspension bridges still requires significant attention.Sidewalk railings,overhaul tracks,and deflectors are known to largely affect such dynamics.Here,the influence of a row of water-filled traffic barriers on the response of a sample suspension bridge is investigated numerically.It is shown that the existence of water barriers causes flow separation and non-negligible vortices with respect to the condition with no water barriers.The vortex shedding frequency at the far end is around 41.30 Hz,relatively close to the real vibration frequency.It is also shown how different incoming angles of attack can change the flow field around the bridge cross-section and the vortex detachment frequency.
基金supported by the Foundation for Innovative Research Groups of National Natural Science Foundation of China(No.51121004)National Natural Science Foundation of China(No.50976026)
文摘A numerical investigation is conducted to explore the evolution of a plasma discharge and its interaction with the fluid flow based on a self-consistent fluid model which couples the discharge dynamics with the fluid dynamics.The effects of the applied voltage on the distribution of velocity and temperature in initially static air are parainetrically studied.Furthermore,the spatial structure of plasma discharge and the resulting force contours in streamwise and normal directions are discussed in detail.The result shows that the plasma actuator produces a net force that should always be directed away from the exposed electrode,which results in an ionic wind pushing particles into a jet downstream of the actuator.When the energy added by the plasma is taken into account,the ambient air temperature is increased slightly around the electrode,but the velocity is almost not affected.Therefore it is unlikely that the induced flow is buoyancy driven.For the operating voltages considered in this paper,the maximum induced velocity is found to follow a power law,i.e.,it is proportional to the applied voltage to the 3.5 power.This promises an efficient application in the flow control with plasma actuators.
文摘A three-porous-medium model for transvascular exchange and extravascular transport of fluid and macromolecules in a spherical solid tumor is developed. The microvasculature , lymphatics, and tissue space are each treated as a porous medium with the flow of blood. lymph, and interstitial fluid obeying Darcy's law and Starling 's assumption. In this part, the role of interstitial pressure and fluid convection are studited. The analytical soiutions are obtained for foe isolated tumor and the normal-tissue-surrounded tumor respectively. The calculated interstitial pressure profue are consistent with the experimental observation that the elevated interstitial pressure is a major barrier in the penetration of macromolecular drug into tumors. The factors which may reduce the interstitial pressure are analyzed in details.
文摘In vertebrates, early brain development takes place at the expanded anterior end of the neural tube, which is filled with embryonic cerebrospinal fluid (E-CSF). We have recently identified a transient blood–CSF barrier that forms between embryonic days E3 and E4 in chick embryos and that is responsible for the transport of proteins and control of E-CSF homeostasis, including osmolarity. Here we examined the presence of glucose transporter GLUT-1 as well the presence of caveolae-structural protein Caveolin1 (CAV-1) in the embryonic blood-CSF barrier which may be involved in the transport of glucose and of proteins, water and ions respectively across the neuroectoderm. In this paper we demonstrate the presence of GLUT-1 and CAV-1 in endothelial cells of blood vessels as well as in adjacent neuroectodermal cells, located in the embryonic blood–CSF barrier. In blood vessels, these proteins were detected as early as E4 in chick embryos and E12.7 in rat embryos, i.e. the point at which the embryonic blood–CSF barrier acquires this function. In the neuroectoderm of the embryonic blood-CSF barrier, GLUT-1 was also detected at E4 and E12.7 respectively, and CAV-1 was detected shortly thereafter in both experimental models. These experiments contribute to delineating the extent to which the blood–CSF embryonic barrier controls E-CSF composition and homeostasis during early stages of brain development in avians and mammals. Our results suggest the regulation of glucose transport to the E-CSF by means of GLUT-1 and also suggest a mechanism by which proteins are transported via transcellular routes across the neuroectoderm, thus reinforcing the crucial role of E-CSF in brain development.
基金supported by National Natural Science Foundation of China (Nos. 51877027 and 51877028)financially supported by the Fundamental Research Funds for the Central Universities (No. DUT20ZD202)+1 种基金the Science and Technology Development Fund of Xinjiang Production and Construction (No. 2019BC009)the Dalian High-Level Talents Innovation and Entrepreneurship Project (No. 2018RQ28)
文摘Both experimental and simulated studies of microdischarge(MD)are carried out in a dielectric barrier discharge with a pin-to-pin gap of 3.5 mm,ignited by a sinusoidal voltage with a peak voltage of 10 kV and a driving frequency of 5 kHz.Statistical results have shown that the probability of the single current pulse in the positive half-period(HP)reaches 73.6%under these conditions.Experimental results show that great luminous intensity is concentrated on the dielectric surface and the tip of the metal electrode.A 1D plasma fluid model is implemented by coupling the species continuity equations,electron energy density equations,Poisson equation,and Helmholtz equations to analyze the MD dynamics on the microscale.The simulated results are in good qualitative agreement with the experimental results.The simulated results show that the MD dynamics can be divided into three phases:the Townsend phase,the streamer propagation phase,and the discharge decay phase.During the streamer propagation phase,the electric field and electron density increase with the streamer propagation from the anode to the cathode,and their maximal values reach 625.48 Td and 2.31×10^(19)m^(-3),as well as 790.13 Td and 3.58×10^(19)m^(-3)in the positive and negative HP,respectively.Furthermore,a transient glow-like discharge is detected around the anode during the same period of streamer propagation.The formation of transient glow-like discharge is attributed to electrons drifting back to the anode,which is driven by the residual voltage in the air gap.