Exploring the Mechanism of Blumea balsamifera(L.)DC in Preventing and Treating Alzheimer's Disease Based on HPLC-ESI-HRMS and Network Pharmacology

[Objectives]To expose the plausible mechanism of Blumea balsamifera(L.)DC.against Alzheimer s disease via network pharmacology and HPLC-ESI-HRMS technology.[Methods]To begin with,HPLC-ESI-HRMS was employed to identify the components of B.balsamifera.Secondly,the potential targets of the components were identified and predicted based on chemical similarity and online databases.Thirdly,by way of topological analysis of a component-disease target interaction network,the primary candidate targets and potential active components were identified.Lastly,molecular docking analysis was used to confirm the interaction between active components and therapeutic targets.[Results]According to the final results,HPLC-ESI-HRMS identified 70 components.Out of these,20 components were potentially biologically active,and most of them were sesquiterpenoids.According to the molecular docking results,the primary active components were appropriately coordinated with the core targets,indicating a high level of pharmacodynamic activity.Thus,the sesquiterpenes present in B.balsamifera are considered potential active ingredients having multi-target and multi-pathway effects for treating Alzheimer s disease.[Conclusions]This research will provide a scientific reference for the future pharmacological activity and clinical application of B.balsamifera.

Effects of Calcium Element on Biomass and Effective Constituents Contents in Blumea balsamifera in Slow Growth Period of Winter

The one-year-old seedlings of Blumea balsamifera （L.） DC. were applied with CaCl2.H2O that supplied Ca in slow growth period of winter three times. The heights, ground diameters, leaf lengths, leaf widths and biomasses of B. balsamifera plants were measured. In addition, the relative contents of total flavones in different parts of B. balsamifera were determined by UV spectrophotometry, and the absolute contents of total flavones were calculated. The relative contents of L-borneol in leaves of B. balsamifera were determined by GC, and the absolute contents of L- borneol were calculated. The results showed that calcium element significantly in- creased the biomasses in leaves, stems and roots of B. balsamifera in slow growth period of winter. The leaf biomass of B. balsamifera in the 5 g/L CaCl2-H2O treat- ment group was significantly higher than those in the other three treatment groups. The leaf biomasses of B. balsamifera in the 10 and 15 g/L CaCl2.H2O treatment groups were significantly higher than that in the CK, with 3.03 and 2.65 times, re- spectively. The application of Ca inhibited the accumulation of total flavones relative contents, but significantly increased the total flavones absolute contents in different parts of B. balsamifera. The relative and absolute contents of L-borneol in the 5 g/L CaCl2 .H2O treatment group were 0.22% and 0.22 g, which were increased by 37.50%, 22.22%, 37.50% and 100%, 100%, 450%, respectively compared with those in the 0, 10 and 15 g/L CaCl2.H2O treatment groups. The Ca element could signifi- cantly promote the accumulation of biomasses in leaves, stems and roots, as well as the absolute contents of total flavones and L-borneol in B. balsamifera in slow growth period of winter.

A New xanthene from Blumea riparia

A new xanthene, named blumeaxanthene, was isolated from Blumea riparia DC. The structure was elucidated on the basis of the data of NMR, MS, IR.

Control Effect of Methanol Extract of Blumea megacephala Chang et Tseng against Turnip Aphid (Lipaphis erysimi Kaltenbach)

[ Objective ] The paper was to explore the application of botanical pesticides on controlling turnip aphid （ Lipaphis erysimi Kaltenbach）. [ Method ] Through potting efficacy experiment of Chinese cabbage, the control effects of two preparations（20% EC and 10% EW）of methanol extract of Blumea megacephala Chang et Tseng against turnip aphid, as well as leaf-protection effect on Chinese cabbage were determined. [ Result] 20% EC of B. megacephala had the highest efficacy in 10-fold dilution, and its control effects against turnip aphid were 57.78%, 77.19% and 84.79% after pesticide spraying for 24, 48 and 72 h; 10% EW of B. megacephala had the highest efficacy in 5-fold dilution, and its control effects against turnip aphid were 74.44%, 84.79% and 89.14% after pesticide spra- ying for 24, 48 and 72 h. The leaf-protection rates of 20% EC orB. megacephala in 10-fold dilution were 89.31% and 94.80% after pesticide spraying for 3 and 5 d; the leaf-protection rates of 10% EW of B. megacephala in 5-fold dilution were 93.50% and 97.83% after pesticide spraying for 3 and 5 d. [ Condusion] The methanol extract of B. megacephala had good control effect against turnip aphid and good protection rate on crops.

Microscopic Structural Comparison between Epidermal Trichomes in Blumea balsamifera (L.) DC. and Blumea laciniata (Roxb.) DC

Immature leaves and stems, which were about one to three centimeters nearby the stem tips, of Blumea balsamifera (L.) DC. (BB) and Blumea laciniata (Roxb.) DC. (BL) were cut into sections under ?18?C with a frozen section machine, and observed under an optical microscope. Results show that the most significant difference is that the BB has only one kind of glandular trichomes, while the BL has two. The glandular trichomes found on BB terms were all short glandular hairs (SGH), which were not longer than 100 μm. On BL stems, besides the SGH, the long glandular hairs (LGH), which were longer than 200 μm, were also found. By the factors pointed out in present study, the BB and BL and be distinguished from each other.

Protective Actions of Blumea Flavanones on Primary Cultured Hepatocytes and Liver Subcellular Organelle against Lipid Peroxidation

To search for the protective actions of blumea flavanones (BFs) on hepatocytes and hepatic subcellular organelle against lipid peroxidation, monkey′s hepatocytes were isolated and cultured with or without blumea flavanones, then damaged by FeSO 4 cysteine or CCl 4. The lipid peroxidation (malondialdehyde production) and alteration in hepatocyte membrane (leakage of GPT) were estimated. Hepatic subcellular organelles were also isolated and incubated with or without blumea flavanones, then injured by FeSO 4 ascorbate. The generation of malondialdehyde(MDA) was measured. It was found that BFs 10 and 100 μmol·L 1 inhibited the MDA generation and GPT (glutamic pyruvic transaminase) leakage out of hepatocytes that were induced by CCl 4 or FeSO 4 cysteine. BFs could prevent lipid peroxidation initiated by FeSO 4 ascorbate in subcellular organelle suspension. Among BFs, BF 2 possessed the strongest activity. Conclusion: Blumea flavanones possess antioxidation activities that protect monkey′s hepatocytes and hepatic subcellular organelle against injuries induced by FeSO4 or CCl 4.

Optimization of Extraction of Total Flavonoids from HERBA BLUMEAE RIPARIAE Using Response Surface Methodology

[Objectives]This study aimed to optimize the ultrasonic-assisted extraction process of total flavonoids from a Zhuang medicine HERBA BLUMEAE RIPARIAE using response surface methodology.[Methods]Using rutin as a reference substance,a standard curve was drawn at a wavelength of 292 nm.Content determination was performed through absorbance under different conditions.The effects of ultrasonic time,ethanol volume fraction and liquid-to-solid ratio on the yield of total flavonoids from HERBA BLUMEAE RIPARIAE were investigated by single-factor tests.By using Box-Behnken test,the ultrasonic-assisted extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was optimized.[Results]The optimal extraction process of total flavonoids from HERBA BLUMEAE RIPARIAE was as follows:ultrasonic time of 30 min,ethanol volume fraction of 50%and liquid-to-solid ratio of 40∶1(mL/g).Under the optimal extraction conditions,the average yield of total flavonoids from HERBA BLUMEAE RIPARIAE was 85.47 mg/g.[Conclusions]The optimized extraction process was convenient and feasible.This study will provide a reference for the extraction of total flavonoids from HERBA BLUMEAE RIPARIAE,and provide certain guidance and basis for further development and utilization of the plan resource of Blumea riparia(Bl.)DC.

Chemical composition and larvicidal effects of essential oil of Blumea martiniana against Anopheles anthropophagus

Objective:To determine chemical composition of the hydrodistilled essential oil of Blumea martiniana,and to evaluate its larvicidal activity against Anopheles anthropophagus,the natural vector of malaria.Methods:Chemical composition of essential oils extracted by steam distillation was investigated by gas chromatography and mass spectroscopy（GC-MS）.Larvicidal activity of essential oil and its four main compounds was carried out by WHO method.Twenty larvae of early fourth-instar stage were used in the larvicidal assay and five replicates were maintained for each concentration.The 24 h LC50 and LC90 values were determined following probit analysis.Results:Totally 68 compounds corresponding to 98.55%of the total oil were identified and the major constituents of essential oil were:linalool（10.36%）,germacrene D（9.09%）, borneol（6.24%）,γ-terpinene（5.38%）.The oil and linalool,germacrene D,bomeol,γ-terpinene exerted significant larvicidal activity with LC50 values of 46.86,35.87,44.61,35.89,and 29.21 mg/L, respectively.It also showed a dose dependent effect on mortality.Conclusions:The essential oil of Blumea martiniana and its four major compounds may have potential for use in control of Anopheles anthropophagus larvae and could be useful in search of newer,safer and more effective natural compounds as larvicides.

Antioxidant Activity of Different Solvent Extracts of Blumea Megacephala in Vitro

[Objectives] The aim was to study the antioxidant activity of different solvent extracts of Blumea Megacephala in vitro. [Methods] The removal rates of DPPH free radicals and ABTS free radicals and the reduction capacity of Fe^(3+) by ethanol extract( EE),acetone extract( AE) and ethyl acetate extract( EAE) of Blumea Megacephala were detected. [Results]The three extracts of Blumea Megacephala had certain antioxidant activity,of which the antioxidant activity of ethyl acetate extract was the strongest and has great potential for development.[Conclusions]The study can provide scientific reference for the deep research on the antioxidant activity of Blumea Megacephala.

Analysis of apigenin in <i>Blumea balsamifera</i>Linn DC. and its inhibitory activity against aldose reductase in rat lens

To investigate the therapeutic potentials of na- tural sources, stepwise polarity fractions of Blumea balsamifera were tested for their ability to inhibit aldose reductase (AR) activity in rat lenses. Of these, the ethyl acetate (EtOAc) fraction exhibited a unique AR inhibitory activity (IC50 value, 0.11 μg/mL). Apigenin was identified from the active EtOAc fraction and exhibited high AR inhibitory activity (IC50 value, 4.03 μM). The content of apigenin was measured in B. balsamifera (0.47 mg/g) by HPLC/UV analysis. Our result suggests that B. balsamifera could be a useful natural source for the development of a novel AR inhibitory agent against diabetic complications.

Morphological Development of Sambong (Blumea balsamifera (L.) DC.) Leaf Studied by Frozen Section and Thin Section

The sambong (Blumea balsamifera (L.) DC.) leaf was investigated by applying frozen section and thin section technology, and observed through optical microscope. The results showed that the sambong leaf was firstly originated nearby the apical bud at the stem tip. The first leaf grows fast, that the mitosis happens frequently and the cytoplasm manufacture rapidly. The cells in the first few leaves have not yet differentiated into the spongy parenchyma and palisade parenchyma. In the mature leaf, the spongy parenchyma and palisade parenchyma were fully developed. The epidermal hairs were firstly developed on the abaxial side of the first leaf.

The Effect of Magnesium on Accumulations of Primary Metabolites and Yield of Blumea balsamifera

[Objectives]This study was conducted to investigate the effects of magnesium on the yield of Blumea balsamifera(L.)DC.and the accumulation of primary metabolites that affect yield of the medicinal material.[Methods]The annual seedlings of B.balsamifera were selected as experimental materials.The treatment concentrations of magnesium(Mg)were set as 0,1.5,15 and 150 mg/ml supplied by MgSO4·7H2O.The yield of the medicinal material was measured dynamically.And the content of total sugar was determined by 3,5-dinitrosalicylic acid colorimetry;the content of crude protein was determined by the Kjeldahl method;the ash content was determined by the high-temperature burning method;the crude fat content was determined with a crude fat instrument;and the crude fiber content was determined by the acid-base washing and weighing method.[Results]Mg significantly increased the yield of B.balsamifera medicinal material,especially 15 mg/ml Mg.It was found that in September,October and November,1.5 mg/ml and 15 mg/ml Mg significantly increased the contents of primary metabolites including total sugar,ash,crude protein,crude fat and crude fiber,and 150 mg/ml of Mg increased the accumulation of total sugar,ash,crude protein and crude fiber to different degrees,but had certain inhibitory effect on the accumulation of crude fat.In December,the application of Mg inhibited the accumulation of total sugar,ash and crude protein to different degrees,but significantly promoted the accumulation of crude fat and fiber.[Conclusions]This study provides a theoretical basis for clarifying the effects of different concentrations of magnesium on plant growth.

Study on the Variation in Main Chemical Composition of Blumea balsamifera in Different Growth Stages

[Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the plant was determined by gas chromatography( GC)with methyl salicylate as internal standard,and total flavonoids were determined by the Aluminum nitrate colorimetry method of Ultraviolet-Visible spectrophotometry. The analysis of variance was used for statistical analysis of the l-borneol and total flavonoids of B. balsamifera in different months and different plant age.[Results]There were differences in chemical ingredients of B. balsamifera in different months of the year.The content of l-borneol in October was the highest,but there was no significant difference from August to December in l-borneol content( P >0. 05). l-borneol content of B. balsamifera in three ages was the highest compared with other ages,and there was a significant difference with B. balsamifera in one year or less( P < 0. 05). Relatively speaking,total flavonoids in June,August and November were higher; total flavonoids content of B. balsamifera in two ages was the highest compared with other ages,and there was a significant difference with other ages( P <0. 05).[Conclusions]If l-borneol is taken as an indicator,for extracting Aipan( l-borneol),it can be harvested from September to midDecember,but October is the best. And it can be harvested at least three years. If the content of total flavonoids is taken as an indicator,it should be harvested in November. And it can be harvested 2-3 years.

Chemical profiling and rapid discrimination of Blumea riparia and Blumea megacephala by UPLC-Q-Exactive-MS/MS and HPLC

Objective:To rapidly identify the two morphologies and chemical properties of similar herbal medicines,Blumea riparia and B.megacephala as the basis for chemical constituent analysis.Methods:UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B.riparia and B.megacephala.Chemical pattern recognition(CPR)was further used to compare and distinguish the two herbs and to identify their potential characteristic markers.Then,an HPLC method was established for quality evaluation.Results:A total of 93 constituents are identified,including 54 phenolic acids,35 flavonoids,two saccharides,one phenolic acid glycoside,and one other constituent,of which 67 were identified in B.riparia and B.megacephala for the first time.CPR indicates that B.riparia and B.megacephala samples can be distinguished from each other based on the LC–MS data.The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B.riparia and B.megacephala.Conclusion:This study demonstrates significant differences between B.riparia and B.megacephala in terms of chemical composition.The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B.riparia and B.megacephala.

艾纳香油通过NF-κB非经典信号影响花生四烯酸代谢缓解LPS所致巨噬细胞的炎症反应

【目的】本文旨在探讨艾纳香油(BBO)通过核转录因子κB(NF-κB)非经典通路影响花生四烯酸(AA)代谢通路发挥抗炎作用。【方法】采用豚鼠离体回肠法检测BBO对慢反应物质(SRS-A)生成的影响,ELISA法检测BBO对脂多糖(LPS)诱导巨噬细胞前列腺素E_(2)(PGE_(2))及白三烯B_(4)(LTB_(4))产生的影响,qRT-PCR检测BBO对LPS诱导巨噬细胞COX-2、5-LOX、FLAP和RelB表达的影响,Western blot检测BBO对NF-κB非经典通路蛋白肿瘤坏死因子受体作用因子3(TRAF3)、肿瘤坏死因子受体作用因子2(TRAF2)、NF-κB诱导激酶(NIK)、p100和RelB浓度的影响。【结果】在1mg·mL^(-1)的BBO药物浓度下减弱了豚鼠回肠的收缩张力(P<0.001),对SRS-A的生成抑制率达到65.34%;与LPS模型组相比,BBO在40-80μg·mL^(-1)浓度下降低AA代谢通路中PGE_(2)(P<0.05)和LTB_(4)(P<0.05)的浓度,降低COX-2(P<0.05)、5-LOX(P<0.05)和FLAP(P<0.05)的表达;此外,40-80μg·mL^(-1)浓度下BBO还降低LPS导致的NF-κB非经典通路中TRAF3(P<0.05)、TRAF2(P<0.05)和NIK(P<0.05)的浓度,进一步抑制p100蛋白的磷酸化(P<0.05),同时抑制转录因子RelB的表达(P<0.05)和RelB蛋白的水平(P<0.05),而BBO自身不引起这些基因与蛋白的变化。【结论】BBO可能通过抑制NF-κB非经典信号中调节蛋白TRAF3和TRAF2,转录因子RelB的浓度,造成NIK蛋白的诱导激酶作用受到抑制,进一步抑制了p100蛋白的磷酸化及其与转录因子RelB的结合,从而影响到下游AA通路中重要限速酶COX-2、5-LOX和FLAP的表达与炎症介质PGE_(2)和LTB_(4)的水平发挥抗炎作用。

滇桂艾纳香提取物促进DUB大鼠子宫收缩机制分析

目的探讨滇桂艾纳香提取物通过促进子宫平滑肌收缩治疗功能失调性子宫出血(DUB)的作用机制。方法利用米非司酮联合米索前列醇建立DUB大鼠模型,成模的雌性大鼠40只,随机分5组:正常妊娠组、DUB模型组、益母草颗粒3.125 g·kg^(-1)组、滇桂艾纳香提取物0.4 g·kg^(-1)组以及0.2 g·kg^(-1)组,另取8只未受孕性成熟雌性大鼠作空白对照组,观察滇桂艾纳香提取物对DUB模型大鼠子宫平滑肌收缩的影响,Western blotting法测定大鼠子宫平滑肌中β_(2)肾上腺素受体(β_(2)AR)、环磷腺苷效应元件结合蛋白(CREB)及p-CREB蛋白表达水平,并检测大鼠子宫平滑肌中蛋白激酶A(PKA)活性和Ca^(2+)离子浓度。结果与DUB模型组相比,滇桂艾纳香提取物可提高大鼠子宫收缩频率(P<0.05),增加子宫平滑肌Ca^(2+)离子浓度,并且增加p-CREB/CREB蛋白表达(P<0.05);滇桂艾纳香提取物还可降低子宫平滑肌PKA活力(P<0.01),但对β_(2)受体表达没有明显影响。结论滇桂艾纳香提取物可能是通过抑制β_(2)受体的活化,间接增强转录调节因子CREB对与子宫平滑肌收缩相关的转录调控,从而促使子宫肌层收缩,产生治疗DUB的作用。

Antihyperglycemic Effect of Various Fractions from Residues of Blumea balsamifera

Objective To evaluate the antihyperglycemic activities of various fractions from theresidues of Blumea balsamifera（BB）,and to properly utilize the waste resource.MethodsThe antihyperglycemic activities were evaluated by the suppression on serum glucose level in vivo andα-glucosidase inhibition assay in vitro.The high-,mid-,and low-dose（1,0.5,and 0.25 g/kg of the herb）fractions were ig given to mice for 8 d.The serum glucose was monitored at 1 and 12 h after feeding.Results The fasting and postprandial serum glucose levels of mice treated with high-dose petroleum ether fraction,ethyl acetate fraction,butyl alcohol fraction,methanol fraction,and water extract from BB were 4.45,4.39,4.43,4.15,3.74 mmol/L and 6.98,6.23,6.45,6.26,5.88 mmol/L,respectively,while those in vehicle control group were 5.63 and 7.50 mmol/L.There are four different inhibiting manners by the results ofα-glucosidase inhibition assay.Conclusion The residues of BB have anti-diabetes activities after steam distillation.

Exploration of Pharmacological and Toxicological Properties of Aerial Parts of Blumea lacera, a Common Weed in Bangladesh

Background:Blumea lacera(Family Asteraceae)is a herb found in the uncultivated lands of many Asian countries.This plant is used by quack doctors for some medicinal purposes.Objective:Works presented in this manuscript were conducted on the ethanol extract of aerial parts of B.lacera.Various pharmacological tests like antioxidant,analgesic,anti-inflammatory,antidiarrheal,diuretic,antibacte-rial,anthelmintic and toxicological tests like brine shrimp lethality and acute toxicity were evaluated.Methods:Antioxidant test was conducted by determining secondary metabolites content and DPPH free radical scavenging assay.Acetic acid-induced writhing method,xylene-induced ear edema method,castor oil-induced diarrheal method,and urinary volume test were performed to evaluate the analgesic,anti-inflammatory,an-tidiarrheal and diuretic effects,respectively.The antibacterial test was performed by disk diffusion method,anthelmintic activity by recording the paralysis and death time of P.cervi and finally,cytotoxic activity was performed by brine shrimp lethality bioassay.Results:TPC,TFC and TTC contents of B.lacera extract were found to be 9 mg GAE/g,31 mg QE/g and 18 mg GAE/g.The SC 50 value of the DPPH radical scavenging assay was found to be higher than 3000μg·mL^(-1).The LD 50 value of B.lacera extract in mice was higher than 5000 mg·kg^(-1).This extract significantly inhibited writhing reflexes by 24.5%and 43.6%and reduced ear edema up to 24.6%and 41%at 250 and 500 mg·kg^(-1) bw doses,respectively.It also increased the latency of the first defecation period up to 52.8 min and 106.6 min as well as decreased the stool count by 34.1%and 48.2%at 250 and 500 mg·kg-1 bw doses,respectively.It also showed an increase in urinary output in the diuretic test.Good antibacterial activity of B.lacera extract was confirmed by retardation in bacterial growth and significantly killed P.cervi in a dose-dependent manner.In brine shrimp lethality bioassay,the LC 50 value was found to be 5.4μg·mL-1.Conclusion:B.lacera showed mild analgesic,anti-inflammatory and antidiarrheal properties.It could be used as a diuretic,antibacterial,anthelmintic and cytotoxic drug which justify the plant’s traditional uses in folk medicine.

艾纳香BbSVP基因的克隆与表达分析

SVP转录因子在调控植物生长发育以及应答非生物胁迫过程中发挥着重要作用。本研究通过同源克隆和RACE技术在艾纳香(Blumea balsamifera)中获得了一个SVP-like基因,并命名为BbSVP。生物信息学分析表明,BbSVP基因的编码区长度为681 bp,编码226个氨基酸,属于MADS-box蛋白家族,蛋白内部无明显信号肽和跨膜结构域。同源性比对显示,BbSVP与同为菊科植物向日葵HaSVP X1的亲缘关系最近。进一步研究发现,BbSVP集中分布于细胞核。BbSVP基因在艾纳香中具有组织表达特异性,在叶片中表达量最高,在花中最低。对艾纳香叶片进行非生物胁迫处理后,RT-qPCR分析显示,BbSVP在转录水平上能够应答PEG、NaCl和4℃低温胁迫。研究表明,BbSVP基因在序列和功能上具有一定的保守性,其可能参与调控艾纳香响应非生物胁迫的过程。

一标多测法同时测定艾纳香药材中6种成分的含量

目的建立一标多测法同时测定艾纳香药材中β-蒎烯、樟脑、芳樟醇、β-石竹烯、α-石竹烯和龙脑6种主要活性成分含量。方法采用气相色谱法,色谱柱为PEG-20M(30 m×0.32 mm,10μm)石英毛细管柱,升温程序:起始温度60℃,维持2 min,以5℃·min^(-1)升至140℃,维持2 min,以2℃·min^(-1)升至150℃。高纯氮气为载气(99999%),火焰离子化检测器和进样口温度均设定为240℃。以龙脑为内参物,建立其与β-蒎烯、樟脑、芳樟醇、β-石竹烯和α-石竹烯的相对校正因子,计算各待测组分的含量,并将测定结果与用内标法测定结果进行比对。结果β-蒎烯、樟脑、芳樟醇、β-石竹烯、α-石竹烯和龙脑分别在2.01~80.4μg·mL^(-1)、6.03~241.2μg·mL^(-1)、2.00~80.0μg·mL^(-1)、6.06~242.4μg·mL^(-1)、4.04~161.6μg·mL^(-1)和80.1~3204μg·mL^(-1)浓度范围内具有良好线性关系,方法平均回收率(n=9)分别为98.4%(RSD=06%)、99.1%(RSD=1.2%)、98.9%(RSD=1.2%)、99.0%(RSD=1.3%),98.8%(RSD=1.5%)和98.5%(RSD=1.1%),与常规内标法比较,一标多测法所得结果无显著性差异。结论该方法简便快速,准确可靠,可用于艾纳香药材的质量控制。