[Objectives]To expose the plausible mechanism of Blumea balsamifera(L.)DC.against Alzheimer s disease via network pharmacology and HPLC-ESI-HRMS technology.[Methods]To begin with,HPLC-ESI-HRMS was employed to identify...[Objectives]To expose the plausible mechanism of Blumea balsamifera(L.)DC.against Alzheimer s disease via network pharmacology and HPLC-ESI-HRMS technology.[Methods]To begin with,HPLC-ESI-HRMS was employed to identify the components of B.balsamifera.Secondly,the potential targets of the components were identified and predicted based on chemical similarity and online databases.Thirdly,by way of topological analysis of a component-disease target interaction network,the primary candidate targets and potential active components were identified.Lastly,molecular docking analysis was used to confirm the interaction between active components and therapeutic targets.[Results]According to the final results,HPLC-ESI-HRMS identified 70 components.Out of these,20 components were potentially biologically active,and most of them were sesquiterpenoids.According to the molecular docking results,the primary active components were appropriately coordinated with the core targets,indicating a high level of pharmacodynamic activity.Thus,the sesquiterpenes present in B.balsamifera are considered potential active ingredients having multi-target and multi-pathway effects for treating Alzheimer s disease.[Conclusions]This research will provide a scientific reference for the future pharmacological activity and clinical application of B.balsamifera.展开更多
[Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the...[Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the plant was determined by gas chromatography( GC)with methyl salicylate as internal standard,and total flavonoids were determined by the Aluminum nitrate colorimetry method of Ultraviolet-Visible spectrophotometry. The analysis of variance was used for statistical analysis of the l-borneol and total flavonoids of B. balsamifera in different months and different plant age.[Results]There were differences in chemical ingredients of B. balsamifera in different months of the year.The content of l-borneol in October was the highest,but there was no significant difference from August to December in l-borneol content( P >0. 05). l-borneol content of B. balsamifera in three ages was the highest compared with other ages,and there was a significant difference with B. balsamifera in one year or less( P < 0. 05). Relatively speaking,total flavonoids in June,August and November were higher; total flavonoids content of B. balsamifera in two ages was the highest compared with other ages,and there was a significant difference with other ages( P <0. 05).[Conclusions]If l-borneol is taken as an indicator,for extracting Aipan( l-borneol),it can be harvested from September to midDecember,but October is the best. And it can be harvested at least three years. If the content of total flavonoids is taken as an indicator,it should be harvested in November. And it can be harvested 2-3 years.展开更多
[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-comp...[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-components by single-marker).[Methods]The method was performed using an Agilent DB-WAX(30 m×0.32 mm,0.25μm)polyethylene glycol chromatographic column;with nitrogen employed as a carrier gas.The constant pressure was 4.73 psi;and the injection temperature was 240℃,with a shunt ratio:of 10:1;The hydrogen flame ion detector with a detector temperature of 240℃;the injection volume was 0.3μL.To verify the accuracy and applicability of QAMS,the results were compared with those obtained using the internal standard method(naphthalene).[Results]In Wanjinxiang Shushuang Ointment,menthol,camphor,1,8-cineole,linalool,borneol,caryophyllene oxide and naphthalene were well separated by the same chromatography with good linearity in their respective ranges(R≥0.9992).The average recoveries were 99.66%,101.03%,98.07%,98.24%,101.39%,and 103.39%with RSD s of 0.69%,1.52%,1.25%,1.94%,1.44%,and 2.74%,respectively.The QAMS is similar to the internal standard method.[Conclusions]This simple,accurate method with high precision,separation and reproducibility can serve as a reference for the quality control of Wanjinxiang Shushuang Ointment.展开更多
OBJECTIVE: To assess the effectiveness of violateoil from Blumea Balsamifera(L.) DC. leaves(BB oil)on wound healing in mice.METHODS: Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield B...OBJECTIVE: To assess the effectiveness of violateoil from Blumea Balsamifera(L.) DC. leaves(BB oil)on wound healing in mice.METHODS: Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield BB oil1/5and BB oil1/10 were applied to the wounded skin before wound healing conditions were assessed by healing rate, histopathology, and contents of collagen, hydroxyproline, and Neuropeptide Substance P(SP). All above results were compared with the efficacies of the control, pure olive oil, basic fibroblast growth factor(BFGF), and cream of Jing Wan Hong(JWH).RESULTS: BB oil1/5and BB oil1/10 improved wound contraction and closure. Histopathology study further confirmed a desirable histological organization of wound tissues. BB oil1/5and BB oil1/10 reduced the number of inflammatory cells, increased wound-healing rates, and significantly increased the hydroxyproline content. Both BB oil1/5and BB oil1/10 improved formation of collagen, and reduced the frequency of fibroblasts. Moreover, BB oil1/5and BB oil1/10 markedly promoted SP expression. However, undiluted BB oil may induce skin thickening and hardening, inhibite collagen synthesis and delay complete skin wound healing.CONCLUSION: The BB oil1/5and BB oil1/10 promoted capillary regeneration, blood circulation, collagen deposition, granular tissue formation, epithelial deposition, and wound contraction. The mechanism underlying the action might be related to induction of SP secretion, and the proliferation and differentiation of mesenchymal cells.展开更多
基金Supported by Science and Technology Project of Guizhou Province of China(QKHPTRC[2021]259,QKH LH[2017]7146)Doctoral Research Initiation Fund(GYZYYFY-BS-2018[14])+2 种基金2018 Guizhou Provincial High Level Innovative Talent ProjectResearch Project on Traditional Chinese Medicine and Ethnic Medicine Science and Technology in Guizhou Province(QZYY2017-079,QZYY2017-087)Project of Key Laboratory Characteristic Forestry of Guizhou Province of China(QJHKY[2021]002).
文摘[Objectives]To expose the plausible mechanism of Blumea balsamifera(L.)DC.against Alzheimer s disease via network pharmacology and HPLC-ESI-HRMS technology.[Methods]To begin with,HPLC-ESI-HRMS was employed to identify the components of B.balsamifera.Secondly,the potential targets of the components were identified and predicted based on chemical similarity and online databases.Thirdly,by way of topological analysis of a component-disease target interaction network,the primary candidate targets and potential active components were identified.Lastly,molecular docking analysis was used to confirm the interaction between active components and therapeutic targets.[Results]According to the final results,HPLC-ESI-HRMS identified 70 components.Out of these,20 components were potentially biologically active,and most of them were sesquiterpenoids.According to the molecular docking results,the primary active components were appropriately coordinated with the core targets,indicating a high level of pharmacodynamic activity.Thus,the sesquiterpenes present in B.balsamifera are considered potential active ingredients having multi-target and multi-pathway effects for treating Alzheimer s disease.[Conclusions]This research will provide a scientific reference for the future pharmacological activity and clinical application of B.balsamifera.
基金Supported by Project of Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(No.1630032017064)
文摘[Objectives]To study the variation in main chemical composition of Blumea balsamifera in different growth stages,and to provide a theoretical basis to determine appropriate harvest period.[Methods]The l-borneol of the plant was determined by gas chromatography( GC)with methyl salicylate as internal standard,and total flavonoids were determined by the Aluminum nitrate colorimetry method of Ultraviolet-Visible spectrophotometry. The analysis of variance was used for statistical analysis of the l-borneol and total flavonoids of B. balsamifera in different months and different plant age.[Results]There were differences in chemical ingredients of B. balsamifera in different months of the year.The content of l-borneol in October was the highest,but there was no significant difference from August to December in l-borneol content( P >0. 05). l-borneol content of B. balsamifera in three ages was the highest compared with other ages,and there was a significant difference with B. balsamifera in one year or less( P < 0. 05). Relatively speaking,total flavonoids in June,August and November were higher; total flavonoids content of B. balsamifera in two ages was the highest compared with other ages,and there was a significant difference with other ages( P <0. 05).[Conclusions]If l-borneol is taken as an indicator,for extracting Aipan( l-borneol),it can be harvested from September to midDecember,but October is the best. And it can be harvested at least three years. If the content of total flavonoids is taken as an indicator,it should be harvested in November. And it can be harvested 2-3 years.
基金Supported by Projects of Guizhou Provincial Department of Education-Growth Program of Young Scientific and Technological Talents in Guizhou Ordinary Higher Education Institutions(QJHKY[2022]264)Rolling Support for Provincial University Research Platform Team Projects(QJJ[2022]010).
文摘[Objectives]To regulate the quality of Wanjinxiang Shushuang Ointment through simultaneously quantifying menthol,camphor,1,8-cineole,linalool,borneol and caryophyllene oxide by QAMS(quantitative analysis of multi-components by single-marker).[Methods]The method was performed using an Agilent DB-WAX(30 m×0.32 mm,0.25μm)polyethylene glycol chromatographic column;with nitrogen employed as a carrier gas.The constant pressure was 4.73 psi;and the injection temperature was 240℃,with a shunt ratio:of 10:1;The hydrogen flame ion detector with a detector temperature of 240℃;the injection volume was 0.3μL.To verify the accuracy and applicability of QAMS,the results were compared with those obtained using the internal standard method(naphthalene).[Results]In Wanjinxiang Shushuang Ointment,menthol,camphor,1,8-cineole,linalool,borneol,caryophyllene oxide and naphthalene were well separated by the same chromatography with good linearity in their respective ranges(R≥0.9992).The average recoveries were 99.66%,101.03%,98.07%,98.24%,101.39%,and 103.39%with RSD s of 0.69%,1.52%,1.25%,1.94%,1.44%,and 2.74%,respectively.The QAMS is similar to the internal standard method.[Conclusions]This simple,accurate method with high precision,separation and reproducibility can serve as a reference for the quality control of Wanjinxiang Shushuang Ointment.
基金the Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences-Tropical Crops Genetic Resources Institute[The Evaluation and Mechanism Research for the Treatment of Ainaxiang(Herba Blumeae Balsamiferae)Oil on Skin Burns Based on Cytokines,No.#1630032014016]the Natural Science Fund of Hainan Province[the Evaluation and Mechanism Research of Ainaxiang(Herba Blumeae Balsamiferae)Oil on Healingand Repairing of Skin Wounds,No.#312022]the Natural Science Fund of China(Study on Variety Classification of"Nalong"and It's Ethanopharmacology of Li in Hainan,No.#81374065)
文摘OBJECTIVE: To assess the effectiveness of violateoil from Blumea Balsamifera(L.) DC. leaves(BB oil)on wound healing in mice.METHODS: Undiluted BB oil and its diluted solutions with olive oil to 1/5 and 1/10 to yield BB oil1/5and BB oil1/10 were applied to the wounded skin before wound healing conditions were assessed by healing rate, histopathology, and contents of collagen, hydroxyproline, and Neuropeptide Substance P(SP). All above results were compared with the efficacies of the control, pure olive oil, basic fibroblast growth factor(BFGF), and cream of Jing Wan Hong(JWH).RESULTS: BB oil1/5and BB oil1/10 improved wound contraction and closure. Histopathology study further confirmed a desirable histological organization of wound tissues. BB oil1/5and BB oil1/10 reduced the number of inflammatory cells, increased wound-healing rates, and significantly increased the hydroxyproline content. Both BB oil1/5and BB oil1/10 improved formation of collagen, and reduced the frequency of fibroblasts. Moreover, BB oil1/5and BB oil1/10 markedly promoted SP expression. However, undiluted BB oil may induce skin thickening and hardening, inhibite collagen synthesis and delay complete skin wound healing.CONCLUSION: The BB oil1/5and BB oil1/10 promoted capillary regeneration, blood circulation, collagen deposition, granular tissue formation, epithelial deposition, and wound contraction. The mechanism underlying the action might be related to induction of SP secretion, and the proliferation and differentiation of mesenchymal cells.
