[ Objective ] The paper was to explore the application of botanical pesticides on controlling turnip aphid （ Lipaphis erysimi Kaltenbach）. [ Method ] Through potting efficacy experiment of Chinese cabbage, the contr...[ Objective ] The paper was to explore the application of botanical pesticides on controlling turnip aphid （ Lipaphis erysimi Kaltenbach）. [ Method ] Through potting efficacy experiment of Chinese cabbage, the control effects of two preparations（20% EC and 10% EW）of methanol extract of Blumea megacephala Chang et Tseng against turnip aphid, as well as leaf-protection effect on Chinese cabbage were determined. [ Result] 20% EC of B. megacephala had the highest efficacy in 10-fold dilution, and its control effects against turnip aphid were 57.78%, 77.19% and 84.79% after pesticide spraying for 24, 48 and 72 h; 10% EW of B. megacephala had the highest efficacy in 5-fold dilution, and its control effects against turnip aphid were 74.44%, 84.79% and 89.14% after pesticide spra- ying for 24, 48 and 72 h. The leaf-protection rates of 20% EC orB. megacephala in 10-fold dilution were 89.31% and 94.80% after pesticide spraying for 3 and 5 d; the leaf-protection rates of 10% EW of B. megacephala in 5-fold dilution were 93.50% and 97.83% after pesticide spraying for 3 and 5 d. [ Condusion] The methanol extract of B. megacephala had good control effect against turnip aphid and good protection rate on crops.展开更多
Objective:To rapidly identify the two morphologies and chemical properties of similar herbal medicines,Blumea riparia and B.megacephala as the basis for chemical constituent analysis.Methods:UPLC-Q-Exactive-MS/MS was ...Objective:To rapidly identify the two morphologies and chemical properties of similar herbal medicines,Blumea riparia and B.megacephala as the basis for chemical constituent analysis.Methods:UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B.riparia and B.megacephala.Chemical pattern recognition(CPR)was further used to compare and distinguish the two herbs and to identify their potential characteristic markers.Then,an HPLC method was established for quality evaluation.Results:A total of 93 constituents are identified,including 54 phenolic acids,35 flavonoids,two saccharides,one phenolic acid glycoside,and one other constituent,of which 67 were identified in B.riparia and B.megacephala for the first time.CPR indicates that B.riparia and B.megacephala samples can be distinguished from each other based on the LC–MS data.The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B.riparia and B.megacephala.Conclusion:This study demonstrates significant differences between B.riparia and B.megacephala in terms of chemical composition.The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B.riparia and B.megacephala.展开更多
文摘[ Objective ] The paper was to explore the application of botanical pesticides on controlling turnip aphid （ Lipaphis erysimi Kaltenbach）. [ Method ] Through potting efficacy experiment of Chinese cabbage, the control effects of two preparations（20% EC and 10% EW）of methanol extract of Blumea megacephala Chang et Tseng against turnip aphid, as well as leaf-protection effect on Chinese cabbage were determined. [ Result] 20% EC of B. megacephala had the highest efficacy in 10-fold dilution, and its control effects against turnip aphid were 57.78%, 77.19% and 84.79% after pesticide spraying for 24, 48 and 72 h; 10% EW of B. megacephala had the highest efficacy in 5-fold dilution, and its control effects against turnip aphid were 74.44%, 84.79% and 89.14% after pesticide spra- ying for 24, 48 and 72 h. The leaf-protection rates of 20% EC orB. megacephala in 10-fold dilution were 89.31% and 94.80% after pesticide spraying for 3 and 5 d; the leaf-protection rates of 10% EW of B. megacephala in 5-fold dilution were 93.50% and 97.83% after pesticide spraying for 3 and 5 d. [ Condusion] The methanol extract of B. megacephala had good control effect against turnip aphid and good protection rate on crops.
基金supported by National Key Research and Development Program of China(No.2018YFC1706101)The Sichuan Key Research and Development Program(No.2021YFS0043)+1 种基金Leading Talent Support Program of National Civil Commission(2021)Special Fund of Natural Science for the Central Universities(No.2020NQN42).
文摘Objective:To rapidly identify the two morphologies and chemical properties of similar herbal medicines,Blumea riparia and B.megacephala as the basis for chemical constituent analysis.Methods:UPLC-Q-Exactive-MS/MS was utilized for profiling and identification of the constituents in B.riparia and B.megacephala.Chemical pattern recognition(CPR)was further used to compare and distinguish the two herbs and to identify their potential characteristic markers.Then,an HPLC method was established for quality evaluation.Results:A total of 93 constituents are identified,including 54 phenolic acids,35 flavonoids,two saccharides,one phenolic acid glycoside,and one other constituent,of which 67 were identified in B.riparia and B.megacephala for the first time.CPR indicates that B.riparia and B.megacephala samples can be distinguished from each other based on the LC–MS data.The isochlorogenic acid A to cryptochlorogenic acid peak area ratio calculated from the HPLC chromatograms was proposed as a differentiation index for distinguishing and quality control of B.riparia and B.megacephala.Conclusion:This study demonstrates significant differences between B.riparia and B.megacephala in terms of chemical composition.The results provide a rapid and simple strategy for the comparison and evaluation of the quality of B.riparia and B.megacephala.
