The nervous system of the silkworm is vital for the development of organisms.It achieves and maintains normal life activities by regulating the function of the organs and all kinds of physiological processes in the si...The nervous system of the silkworm is vital for the development of organisms.It achieves and maintains normal life activities by regulating the function of the organs and all kinds of physiological processes in the silkworm.BmApontic(BmApt),as an imports nt bZIP tran scripti on factor,is required for the formatio n of pigme ntation in the silkworm.However,the fun ction of BmApt in the development of the nervous system of the silkworm remains unclear.Here,we showed that amino acid seque nee of BmApt was evoluti on arily con served in its Myb/SANT motif and basic DNA bindi ng domain.BmApt was expressed in the nervous system at the embry onic stage.Knockdow n of Bmapt by RNA interfere nee resulted in abno rmal development of axons.Moreover,the expression of BmnetrinA,BmnetrinB and Bmfrazzled was decreased in the Bmapt knockdown embryos.These results dem on strate that BmApt controls neurodevelopme nt by activati ng the expressi on of Bmnetrin and Bmfrazzled.展开更多
基金This work was supported by the National Natural Science Foundation of China(31571502,31872971 and 31602011),the Funds of"Shandong Double Tops*1 Program,China(SYL2017YSTD09)and the earmarked fund for China Agriculture Research System(CARS-22).
文摘The nervous system of the silkworm is vital for the development of organisms.It achieves and maintains normal life activities by regulating the function of the organs and all kinds of physiological processes in the silkworm.BmApontic(BmApt),as an imports nt bZIP tran scripti on factor,is required for the formatio n of pigme ntation in the silkworm.However,the fun ction of BmApt in the development of the nervous system of the silkworm remains unclear.Here,we showed that amino acid seque nee of BmApt was evoluti on arily con served in its Myb/SANT motif and basic DNA bindi ng domain.BmApt was expressed in the nervous system at the embry onic stage.Knockdow n of Bmapt by RNA interfere nee resulted in abno rmal development of axons.Moreover,the expression of BmnetrinA,BmnetrinB and Bmfrazzled was decreased in the Bmapt knockdown embryos.These results dem on strate that BmApt controls neurodevelopme nt by activati ng the expressi on of Bmnetrin and Bmfrazzled.
