As an important commercial species, the blue swimming crabs with different body-colors were discovered along the coast of East China in recent years. Both purple and tea-green crabs have similar, even the same physica...As an important commercial species, the blue swimming crabs with different body-colors were discovered along the coast of East China in recent years. Both purple and tea-green crabs have similar, even the same physical characteristics except for the carapace color. In order to identify the relationships between them, we amplified and compared two conserved mitochondrial gene fragments of color-different crab individuals through PCR-SSCP and DNA sequencing analyses. The results showed that there was no distinct variation of DNA bands of both the COl and 16srRNA genes detected in polyacrylamide gels through SSCP analysis between purple and tea-green crab samples, and the following sequence analysis of color-different crab individuals presented 99.81% and 99.91% nucleotide sequence identity of the COl and 16srRNA genes respectively, which indicated that there was no species or subspecies differentiation between them. In addition, the purple individuals were more conserved in DNA sequence in compadson with that of the tee-green ones.展开更多
The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals we...The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis （BSA） and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei＇s gene diversity was 0.375 6, and Shannon＇s information index was 0.554 5 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei＇s gene diversity was 0.3451, and Shannon＇s information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers （SBS A09 and SBS G16） got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.展开更多
Given the morphological characters of purple crabs identical with that of the tea-green ones except the body color, it is expected that the purple crabs and the tea-green ones were from the same species. In order to i...Given the morphological characters of purple crabs identical with that of the tea-green ones except the body color, it is expected that the purple crabs and the tea-green ones were from the same species. In order to identify the phylogenetic relationships between them at the molecular level, we amplified and compared two conserved mitochondrial gene fragments of purple and tea-green crabs through PCR-SSCP and DNA sequencing analyses. The results showed that there was no distinct variation of DNA bands of both the CO1 and 16srRNA genes detected in polyacrylamide gels through SSCP analysis between purple and tea-green crab samples, and the following sequence analysis of color-different crab individuals presented 99.81% and 99.91% nucleotide sequence identity of the CO1 and 16srRNA genes respectively. These indicated that there was no species or subspecies differentiation between purple and tea-green crabs, meaning that they belonged to the same species, Portunus trituberculatus.展开更多
BdlR strain killifish Oryzias latipes, which can be found in natural populations in Kochi Prefecture, Japan, exhibit a fading body-color. The aim of this article is trace of body fade by enzymatic analysis especially ...BdlR strain killifish Oryzias latipes, which can be found in natural populations in Kochi Prefecture, Japan, exhibit a fading body-color. The aim of this article is trace of body fade by enzymatic analysis especially blacking key enzyme tyrosinase. A single scale was collected from a single specimen of this strain at the dorsal axis, and the supernatant fi-action developed from this sample served as a tyrosinase extract. The extract fraction showed low tyrosinase activity when L-tyrosine, L-dopa and L-dopamine were used as enzyme substrates. After addition of L-cysteine to the sample scale, surface color gradually faded as pheomelanin formed. Tyrosinase was believed to have been in the folded phase and especially active arotmd the catalytic site and copper was detected in the scale. In hereafter, molecular analysis can attain above purpose.展开更多
基金supported by the grant of the Key Project in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (2006BAD09A01)
文摘As an important commercial species, the blue swimming crabs with different body-colors were discovered along the coast of East China in recent years. Both purple and tea-green crabs have similar, even the same physical characteristics except for the carapace color. In order to identify the relationships between them, we amplified and compared two conserved mitochondrial gene fragments of color-different crab individuals through PCR-SSCP and DNA sequencing analyses. The results showed that there was no distinct variation of DNA bands of both the COl and 16srRNA genes detected in polyacrylamide gels through SSCP analysis between purple and tea-green crab samples, and the following sequence analysis of color-different crab individuals presented 99.81% and 99.91% nucleotide sequence identity of the COl and 16srRNA genes respectively, which indicated that there was no species or subspecies differentiation between them. In addition, the purple individuals were more conserved in DNA sequence in compadson with that of the tee-green ones.
基金supported by the grant of the Key Project in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period (No. 2006BAD09A01)
文摘The blue swimming crabs Portunus trituberculatus with tea-green and/or purple carapace color have been discovered on the shore of eastern part of China these days, In this paper, 40 color-different crab individuals were evaluated using 80 RAPD primers. 30 primers were selected using bulk segregation analysis （BSA） and RAPD-PCR analysis. The results showed that the ratio of polymorphic loci was 61.29%, and Nei＇s gene diversity was 0.375 6, and Shannon＇s information index was 0.554 5 among purple crab individuals; and the ratio of polymorphic loci was 62.44%, and Nei＇s gene diversity was 0.3451, and Shannon＇s information index was 0.5099 among tea-green crab individuals. It was clear that the genetic diversity between purple and tea-green crabs was similar, which indicated close phylogenetic relationships between the purple and tea-green crabs. In addition, two primers （SBS A09 and SBS G16） got specific DNA bands by comparing forty individuals of color-different crab individuals through RAPD-PCR analysis and the following electrophoresis. They indicated close phylogenetic relationships between purple and tea-green crabs.
文摘Given the morphological characters of purple crabs identical with that of the tea-green ones except the body color, it is expected that the purple crabs and the tea-green ones were from the same species. In order to identify the phylogenetic relationships between them at the molecular level, we amplified and compared two conserved mitochondrial gene fragments of purple and tea-green crabs through PCR-SSCP and DNA sequencing analyses. The results showed that there was no distinct variation of DNA bands of both the CO1 and 16srRNA genes detected in polyacrylamide gels through SSCP analysis between purple and tea-green crab samples, and the following sequence analysis of color-different crab individuals presented 99.81% and 99.91% nucleotide sequence identity of the CO1 and 16srRNA genes respectively. These indicated that there was no species or subspecies differentiation between purple and tea-green crabs, meaning that they belonged to the same species, Portunus trituberculatus.
文摘BdlR strain killifish Oryzias latipes, which can be found in natural populations in Kochi Prefecture, Japan, exhibit a fading body-color. The aim of this article is trace of body fade by enzymatic analysis especially blacking key enzyme tyrosinase. A single scale was collected from a single specimen of this strain at the dorsal axis, and the supernatant fi-action developed from this sample served as a tyrosinase extract. The extract fraction showed low tyrosinase activity when L-tyrosine, L-dopa and L-dopamine were used as enzyme substrates. After addition of L-cysteine to the sample scale, surface color gradually faded as pheomelanin formed. Tyrosinase was believed to have been in the folded phase and especially active arotmd the catalytic site and copper was detected in the scale. In hereafter, molecular analysis can attain above purpose.
