Boeravinone B ameliorates allergic nasal inflammation by modulating the GATA-3/T-bet signaling pathway in a mouse model of allergic rhinitis

Objective:To evaluate the anti-allergic effect of boeravinone B against ovalbumin-induced allergic rhinitis in mice and explore its possible mechanism.Methods:For the induction of allergic rhinitis,mice were intraperitoneally sensitized and intranasally challenged with ovalbumin,as well as orally received various concentrations of boeravinone B.Nasal mucosal inflammation,and the levels of nitric oxide,β-hexosaminidase,IFN-γ,LTC-4,myeloperoxidase,Nrf2,HO-1,GATA-3,ROR-γ,T-bet,antioxidant parameters,and allergen-specific cytokines were assessed.Results:Boeravinone B markedly reduced ovalbumin-induced increase in the number of episodes of nasal sneezing,rubbing,and discharge,as well as the levels of IgE,IgG1,andβ-hexosaminidase(P<0.05).It also significantly reduced differential cell count,myeloperoxidase,oxide-nitrosative stress,and the levels of IL-1β,IL-4,IL-5,IL-6,IL-13,IL-17,tumor necrosis factor-α,GATA-3,and ROR-γwhile enhancing the level of T-bet.Conclusions:Boeravinone B is a potential therapeutic agent for allergic rhinitis by modulating various inflammatory mediators and immune responses.

喜马拉雅紫茉莉根中Boeravinone C含量的HPLC分析

建立HPLC测定喜马拉雅紫茉莉[Mirabilis himalaica（Edgew.）Heim.]药材中Boeravinone C含量的方法。色谱柱为Kromosil 100-5 C18柱（150 mm×4.6 mm,5μm）,流动相为甲醇-水（70∶30,V/V）,流速为0.8 m L/min,检测波长294 nm。结果表明,Boeravinone C含量在0.032 4-0.486 0μg范围内,线性关系良好（R^2=0.999 9）;精确度和准确度良好,平均回收率为97.2%。该方法快速、简单,可用于喜马拉雅紫茉莉药材的质量评价。

一年生喜马拉雅紫茉莉根的产量和boeravinone C的动态变化

目的确定一年生喜马拉雅紫茉莉的最佳采收时期。方法利用HPLC研究了不同采收期对一年生喜马拉雅紫茉莉根的产量和boeravinone C含量的影响。结果林芝地区一年生喜马拉雅紫茉莉根的最佳采收时期是9月20日左右。结论为喜马拉雅紫茉莉的人工种植与示范提供指导,从源头上保证喜马拉雅紫茉莉药材的质量和产量。

紫茉莉酰胺:喜马拉雅紫茉莉中一新桂皮酰胺

从喜马拉雅紫茉莉(Mirablis himalaica)根的乙醇提取物中分离到 新柱皮酰胺,即紫茉莉酰胺和一己知化合物boeravinone E经波谱分析确定了它们的结构。