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BMP亚家族成员中影响绵羊繁殖性能的基因研究进展
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作者 刘梦兰 邵旭文 +1 位作者 单雪松 姜怀志 《家畜生态学报》 北大核心 2024年第7期1-8,共8页
排卵数和产羔数是反映绵羊繁殖性状的关键性指标,也是绵羊重要的经济性状,二者属于中低遗传力性状,受基因型、环境和营养状况等多重因素共同影响。由BMP、GDF和AMH等组成的BMP亚家族是TGF-β超家族的重要组成部分,BMPR1B、BMP15和GDF9... 排卵数和产羔数是反映绵羊繁殖性状的关键性指标,也是绵羊重要的经济性状,二者属于中低遗传力性状,受基因型、环境和营养状况等多重因素共同影响。由BMP、GDF和AMH等组成的BMP亚家族是TGF-β超家族的重要组成部分,BMPR1B、BMP15和GDF9是目前与绵羊繁殖性能相关性研究最为深入的基因。该文对BMP亚家族成员中提升绵羊排卵数及产羔数的基因的SNP位点进行归纳,总结了这些基因影响繁殖性能的SNP位点的发现,品种、定位、表型,以及对排卵数和产羔率的影响机制,旨在为绵羊多胎品种的选育和高效繁殖提供理论参考。 展开更多
关键词 突变位点 繁殖性状 bmpS 主效基因 绵羊
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针刀干预对LDH模型大鼠TGF-β/BMP信号通路水平的影响
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作者 钟志年 靳康 +2 位作者 熊俊 孙岑 聂容荣 《河北医药》 CAS 2024年第17期2570-2576,共7页
目的探讨针刀干预对腰椎间盘突出症(LDH)模型大鼠椎间盘退变及转化生长因子β/骨形态发生蛋白(TGF-β/BMP)信号通路水平的影响。方法建立LDH大鼠模型,大鼠分为空白组、假手术组、模型组、塞来昔布组(0.34g·kg^(-1)·d^(-1)塞... 目的探讨针刀干预对腰椎间盘突出症(LDH)模型大鼠椎间盘退变及转化生长因子β/骨形态发生蛋白(TGF-β/BMP)信号通路水平的影响。方法建立LDH大鼠模型,大鼠分为空白组、假手术组、模型组、塞来昔布组(0.34g·kg^(-1)·d^(-1)塞来昔布溶剂)、针刀组、TGF-β/BMP抑制剂组(针刀+SB-431542,针刀+100μL/鼠/2d的SB431542)、TGF-β/BMP激动剂组(针刀+SRI-011381 hydrochloride,针刀+10 mg·kg^(-1)·2 d^(-1)的SRI-011381 hydrochloride),每组20只。观察7组大鼠一般行为状态;HE染色观察椎间盘软骨组织形态学变化;RT-qPCR检测TGF-β1 mRNA、BMP-7 mRNA、Smad2/3 mRNA、Smad1/5/8 mRNA、Smad4 mRNA及TIMP-3 mRNA的表达;ELISA法检测肿瘤坏死因子(TNF-α)、白介素-6(IL-6)、基质金属蛋白酶-3(MMP-3)水平;免疫组化检测椎间盘软骨组织TIMP-3表达;Western blot检测TGF-β1、BMP-7、Smad2/3、Smad1/5/8、BMPR-1A、ALK5、Sox9、COL2A1蛋白水平。结果与空白组及假手术组比较,模型组大鼠行为异常,椎间盘出现破裂,基质丢失,软骨细胞减少,软骨终板与髓核边界结构紊乱,Smad4 mRNA、TIMP-3 mRNA表达与TGF-β1、BMP-7、Smad2/3、Smad1/5/8 mRNA及蛋白、BMPR-1A、ALK5、Sox9、COL2A1蛋白表达、TIMP-3平均光密度值显著降低(P<0.05),TNF-α、IL-6、MMP-3水平显著升高(P<0.05)。与模型组比较,针刀组、塞来昔布组大鼠异常行为减少,椎间盘软骨组织损伤减轻,Smad4 mRNA、TIMP-3 mRNA表达与TGF-β1、BMP-7、Smad2/3、Smad1/5/8 mRNA及蛋白、BMPR-1A、ALK5、Sox9、COL2A1蛋白表达、TIMP-3平均光密度值显著升高(P<0.05),TNF-α、IL-6、MMP-3水平显著降低(P<0.05)。与针刀组比较,TGF-β/BMP抑制剂组大鼠异常行为及椎间软骨组织损伤加重;Smad4 mRNA、TIMP-3 mRNA表达与TGF-β1、BMP-7、Smad2/3、Smad1/5/8 mRNA及蛋白、BMPR-1A、ALK5、Sox9、COL2A1蛋白表达、TIMP-3平均光密度值显著降低(P<0.05),TNF-α、IL-6、MMP-3水平显著升高(P<0.05)。TGF-β/BMP激动剂组大鼠异常行为减少,椎间盘软骨组织损伤减轻;Smad4 mRNA、TIMP-3 mRNA表达与TGF-β1、BMP-7、Smad2/3、Smad1/5/8 mRNA及蛋白、BMPR-1A、ALK5、Sox9、COL2A1蛋白表达、TIMP-3平均光密度值显著升高(P<0.05),TNF-α、IL-6、MMP-3水平显著降低(P<0.05)。结论针刀干预可能通过激活TGF-β/BMP信号通路,抑制细胞外基质降解、抑制椎间盘组织内炎症,起到延缓椎间盘退变发展、达到治疗LDH的目的。 展开更多
关键词 针刀干预 腰椎间盘突出症 椎间盘退变 TGF-β/bmp信号通路
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血管钙化抑制因子BMP-7的研究进展
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作者 周鑫 邢露 +9 位作者 李鹏权 赵栋 褚海清 贺春霞 秦魏 李慧瑾 付佳 张烨 肖丽 曹慧玲 《中国药理学通报》 CAS CSCD 北大核心 2024年第7期1226-1230,共5页
血管钙化是一个主动可调控的心血管系统异位钙化过程,目前尚无可逆转的有效干预手段。血管钙化和骨形成具有共同的调控机制,骨形态发生蛋白7(bone morphogenetic protein 7,BMP-7)可维持血管平滑肌细胞的收缩表型抑制血管钙化。同时,BM... 血管钙化是一个主动可调控的心血管系统异位钙化过程,目前尚无可逆转的有效干预手段。血管钙化和骨形成具有共同的调控机制,骨形态发生蛋白7(bone morphogenetic protein 7,BMP-7)可维持血管平滑肌细胞的收缩表型抑制血管钙化。同时,BMP-7可刺激成骨细胞分化过程,增加骨形成,减少骨吸收降低异位钙化压力。该文系统综述BMP-7影响血管钙化的作用与机制及其目前的临床应用现状。这将有助于以BMP-7为新的分子标志物与潜在药物靶标,研发早期诊断试剂盒及治疗药物,以期实现血管钙化的早期预防与干预,改善患者预后。 展开更多
关键词 骨形态发生蛋白7 血管钙化 骨形成 血管平滑肌细胞 分泌蛋白 分子机制
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Improved Osteointegration of Ti-6Al-4V-implants of Different Surface Texture by the Use of Bone Morphogenetic Protein-3 (BMP-3)
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作者 Esenwein S A Esenwein S +3 位作者 Herr G Hauser J Muhr G Hartwig C-H 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2005年第B12期301-303,共3页
Half of altogether 60 cylindrical implant devices made of titanium-aluminum-vanadium alloy ( Ti-6Al-4V) were plusna-sprayed with a hydroxyapatite-couting and the other half had a corundum blasted porous surface. 15... Half of altogether 60 cylindrical implant devices made of titanium-aluminum-vanadium alloy ( Ti-6Al-4V) were plusna-sprayed with a hydroxyapatite-couting and the other half had a corundum blasted porous surface. 15 implants of each group of the titanium test buplants were coated with 230 μg porcine, high-purified BMP- 3-precipitute per implant. In each case a BMP- 3-couted and an uncoated control-device were implanted into the femoral part of the putellofemoral joint of the right and left leg of 30 adult giant rabbits. Histomorphological and histomorphometrical we found in both groups with BMP- 3-coated test devices an improved osteointegrution. Stutistical evaluation using the t-test for matched samples showed 5 weeks after surgery a significant higher volume of tony formed bone of the BMP- 3-coated corundum- blasted or hydroxyapathe- coated Ti- 6Al- 4 V test devices compared to the non-couted controls of the same t)pe (p 〈 0.01, t-test for matched samples). In both implant groups with BMP-couting a synergetic effect was verifiable although the bone ongrowth in the hydroxyaputite coated implants was more extensive than in the corundum blasted implants. Light microscopy demonstrated osteointegrution without connective tissue membrane around the surface of the implants. Our results indicate that composite metal implants,as used in endoprosthetics and implantology , are suitable carriers for BMP- 3 and im proved fixation of the implants can be achieved. The hydroxyapatite surface is superior to the corundum-blasted surface with regards to the observed parameters because of its pronounced bioactivity and its osteoconductive characteristics. 展开更多
关键词 bone morphogenetic protein-3 bmp- 3 hydroxyapatite OSTEOINTEGRATION surface coating bone ingrowth IMPLANTOLOGY
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骨质疏松性椎体压缩性骨折患者血清BMP-2,Runx2水平对术后骨折延迟愈合的预测价值
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作者 王金龙 冯大军 周全 《新疆医科大学学报》 CAS 2024年第6期836-842,共7页
目的探讨骨质疏松性椎体压缩性骨折(Osteoporotic vertebral compressibility fracture,OVCF)患者血清骨形态发生蛋白-2(Bone morphogenetic protein-2,BMP-2)、核心结合因子2(Runt-related transcription factor 2,Runx2)水平对术后骨... 目的探讨骨质疏松性椎体压缩性骨折(Osteoporotic vertebral compressibility fracture,OVCF)患者血清骨形态发生蛋白-2(Bone morphogenetic protein-2,BMP-2)、核心结合因子2(Runt-related transcription factor 2,Runx2)水平对术后骨折延迟愈合的预测价值。方法选取2020年1月-2023年4月徐州医科大学附属淮安医院骨科收治的260例OVCF患者为研究对象,均采用经皮椎体成形术(Percutaneous vertebroplasty,PVP)或椎体后凸成形术(Percutanous kyphoplasty,PKP)治疗,根据术后3个月骨折愈合情况分延迟愈合组、正常愈合组。分析OVCF患者术后骨折延迟愈合的影响因素及血清BMP-2,Runx2水平对术后骨折延迟愈合的预测价值。绘制决策曲线和临床影响曲线,评价血清BMP-2,Runx2水平对术后骨折延迟愈合的价值。结果260例患者术后无失访,22例出现骨折延迟愈合,发生率为8.46%(22/260)。延迟愈合组年龄、吸烟比率、合并糖尿病比率、合并甲状腺疾病比率、有椎体裂隙征比率大于正常愈合组,骨密度T值、术前和术后3个月血清BMP-2,Runx2水平小于正常愈合组,差异有统计学意义(P<0.05)。合并糖尿病、有椎体裂隙征是OVCF患者术后骨折延迟愈合的独立危险因素,骨密度T值、BMP-2,Runx2是OVCF患者术后骨折延迟愈合的独立保护因素(P<0.05)。BMP-2,Runx2联合预测OVCF患者术后骨折延迟愈合对应AUC值为0.856。阈值在0.10~0.63范围内,BMP-2,Runx2联合评估模型评估OVCF患者术后骨折延迟愈合的净受益率优于单独检测。在阈值概率为0.26时,被该模型划分入高风险的人数与真阳性人数基本达成一致。结论OVCF患者血清BMP-2,Runx2水平降低,二者联合检测对术后骨折延迟愈合具有较高的预测效能。 展开更多
关键词 骨质疏松性椎体压缩性骨折 骨形态发生蛋白-2 核心结合因子2 术后骨折延迟愈合
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AN IMMUNOCYTOCHEMICAL STUDY OF BONE MORPHOGENETIC PROTEIN IN EXPERIMENTAL FRACTURE HEALING OF THE RABBIT MANDIBLE 被引量:4
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作者 金岩 杨连甲 FrankH.White 《Chinese Medical Sciences Journal》 CAS CSCD 1994年第2期91-95,共5页
A monoclonal antibody raised against bone morphogenetic protein (BMP-McAb) has been used to demonstrate the presence of bone morphogenetic protein(BMP) in experimental fracture healing. Rabbit mandibles were fractured... A monoclonal antibody raised against bone morphogenetic protein (BMP-McAb) has been used to demonstrate the presence of bone morphogenetic protein(BMP) in experimental fracture healing. Rabbit mandibles were fractured using standardized methods and left to heal for 3, 7, 14, 21 and 24 d, respectively. The avidin-biotin complex (ABC) method demonstrated an accumulation of positively stained primitive mesenchymal cells at the fracture site in the hematoma stage of bone repair. These cells appeared to undergo differentiation into positively-stained chondroblasts and osteoblasts during the phase of callus formation. Undifferentiated mesenchymal cells showed a high positive reactivity in the early post-fracture stages but a much lower reactivity during the remodelling phase.The results of our study suggest that bone inductive processes are accompanied by the presence of BMP in osteoprogenitor cells during fracture healing of the mandible and that BMP may play a significant role in osteogenesis during bone healing. 展开更多
关键词 bone morphogenetic protein(bmp) bone induction bone fracture healing
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The Effect of Simvastatin on mRNA Expression of Transforming Growth Factor-β1,Bone Morphogenetic Protein-2 and Vascular Endothelial Growth Factor in Tooth Extraction Socket 被引量:10
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作者 Chang Liu Zhe Wu Hong-chen Sun 《International Journal of Oral Science》 SCIE CAS CSCD 2009年第2期90-98,共9页
Aim To determine the effect of local simvastatin application on the mRNA expression level of transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (... Aim To determine the effect of local simvastatin application on the mRNA expression level of transforming growth factor-β1 (TGF-β1), bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) in the tooth sockets of rat. Methodology Forty-eight male Wistar rats were randomly divided into experimental and control groups (n=24). Polylactic acid/polyglycolic acid copolymer carriers, with or without simvastatin, were implanted into extraction sockets of right mandibular incisors. The expression of TGF-β1, BMP-2 and VEGF mRNA was determined by in situ hybridization in the tooth extraction socket at five days, one week, two weeks and four weeks after implantation. Results The fusiform stroma cells in the tooth extraction socket began to express TGF-β1, BMP-2 and VEGF mRNA in both experimental and control groups from one week after tooth extraction until the end of experiment. The expression of TGF-131 and BMP-2 mRNA in the experimental group was significantly up-regulated after one, two and four weeks, and expression of VEGF mRNA was significantly increased after one and two weeks compared with that in the control group. Conclusion The findings indicate that local administration of simvastatin can influence alveolar bone remodeling by regulating the expression of a school of growth factors which are crucial to osteogenesis in the tooth extraction socket. 展开更多
关键词 bone morphogenetic protein-2 bmp-2) in situ hybridization SIMVASTATIN tooth extraction socket transforming growth factor-β1 (TGF-β1) vascular endothelial growth factor (VEGF)
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Influence of bone morphogenetic protein on articular cartilage regeneration following periosteal grafting
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作者 Yimin Zhang Xin Jiang Yongzhi Guo 《Journal of Biomedical Science and Engineering》 2013年第1期25-30,共6页
Background: Autologous periosteal grafting is used as treatment for articular cartilage defect. Objective: To study the effect of bone morphogenetic protein (BMP) on articular cartilage regeneration following perioste... Background: Autologous periosteal grafting is used as treatment for articular cartilage defect. Objective: To study the effect of bone morphogenetic protein (BMP) on articular cartilage regeneration following periosteal grafting. Methods: 16 healthy 15 week-old New Zealand white rabbits of both sexes (32 knees) were randomly divided into experimental group (group A) and control group (group B). A4.0 mmdiameter full-thickness articular cartilage defect was created in the femoral intercondylar fossa in all rabbits. Following this, a4.0 mmdiameter section of the periosteum was harvested from the anteromedial part of the upper tibial bone. In group A (eight rabbits, 16 knees), the cartilage defect was covered with periosteum, into which 20 μg BMP and 20% Pluronic were injected. In group B (eight rabbits, 16 knees), the cartilage defect was covered with periosteum, into which the same dosage of 0.9% NS (Normal saline) and 20% Pluronic were injected. All rabbits were sacrificed at 4, 8, and 12 weeks postoperatively, the cartilage defect areas were examined macroscopically and microscopically, and the morphology of the chondrocytes and collagen fibers were examined by scanning electron microscopy. Results: The filling of the defects with regenerated tissue was observed in both the group. The most notable improvement was that the cartilage regeneration in group A was obviously superior to that in group B, with the total histological score in group A significantly higher. Conclusion: BMP is an effective factor that could promote regeneration of articular cartilage and lead to successful cartilaginous resurfacing following periosteal 展开更多
关键词 bone Morphogenetic protein (bmp) ARTICULAR CARTILAGE REGENERATION PERIOSTEAL GRAFTING
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Human bone morphogenetic protein-2 gene transfer induces human mesenchymal stem cell proliferation and differentiation in vitro
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作者 李军 范清宇 +3 位作者 钱济先 马保安 周勇 张明华 《Journal of Medical Colleges of PLA(China)》 CAS 2004年第2期115-119,共5页
Objective: To identify eukaryotic expression vector of human bone morphogenetic protein 2 pcDNA3/BMP2, verify its expression in transfected human mesenchymal stem cells (hMSCs) and the effect on hMSCs differentiation.... Objective: To identify eukaryotic expression vector of human bone morphogenetic protein 2 pcDNA3/BMP2, verify its expression in transfected human mesenchymal stem cells (hMSCs) and the effect on hMSCs differentiation. Methods: The BMP2 gene was cloned into a eukaryotic expression vector pcDNA3. Transfected the recombinant into hMSCs by liposome. Immunnohistochemistry and in situ hybridization methods were used to identify the expression of BMP2 mRNA and protein; ALP and Von Kossa stains were performed to identify the BMP2 gene differentiated effect on the hMSCs. Results: The pcDNA3/BMP2 fragments were as large as theory. BMP2 mRNA and protein were expressed and synthesized both in 48 h and 4 weeks after transfection, the ALP and Ca deposit exhibition, which marked the osteogenic lineage of hMSCs, were enhanced and sped. Conclusion: Transfection of pcDNA3/BMP2 is able to provide transient and persistent expression in hMSCs, and promote the MSCs differentiation to osteogenic lineage. 展开更多
关键词 bone morphogenetic protein(bmp2) TRANSFECTION human mesenchymal stem cells (hMSCs) osteogenic differentiation
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AB004. Regulation of retinal angiogenesis and vascular permeability by bone morphogenetic protein signaling
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作者 Bruno Larrivée 《Annals of Eye Science》 2018年第1期410-410,共1页
The bone morphogenetic protein(BMP)family of proteins has a multitude of roles throughout the body.It plays important roles in development and in the adult vascular endothelium,by modulating the angiogenic response.Th... The bone morphogenetic protein(BMP)family of proteins has a multitude of roles throughout the body.It plays important roles in development and in the adult vascular endothelium,by modulating the angiogenic response.The endothelial-specific receptor BMP receptor Alk1 is of particular importance in the proper remodeling of the vasculature and its ligand BMP9 has been shown to be a potent inhibitor of neovascularization.Dysregulated BMP signaling has been linked to multiple vascular diseases and can lead to the abnormal angiogenesis.We therefore investigated the role of BMP9/Alk1 signaling in retinal angiogenesis,and its therapeutic implications for vascular pathologies of the eye. 展开更多
关键词 Retinal angiogenesis bone morphogenetic protein(bmp) vascular permeability
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周期性牵张力通过调控BMP2对人牙周膜成纤维细胞成骨分化的初步研究 被引量:1
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作者 张超 谢倩倩 +1 位作者 许家顺 胡静 《锦州医科大学学报》 CAS 2023年第4期22-26,共5页
目的探究周期性牵张力对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLFs)中骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)表达的影响及对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDL... 目的探究周期性牵张力对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLFs)中骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)表达的影响及对人牙周膜成纤维细胞(human periodontal ligament fibroblasts,hPDLFs)成骨分化的作用。方法体外培养hPDLFs,分为3组(空白对照组、6 h组、12 h组),分别不加力、加力6 h和12 h。使用免疫荧光实验检测3组细胞骨架蛋白表达情况;使用Western Blot实验检测3组BMP-2表达情况;碱性磷酸酶染色试剂盒及茜素红染色实验分别用于检测3组碱性磷酸酶(alkaline phosphatase,ALP)活性及骨钙结节形成情况。结果免疫荧光实验结果显示6 h组、12 h组hPDLFs骨架蛋白表达明显高于空白对照组;Western Blot实验结果显示6 h组、12 h组BMP-2蛋白表达明显高于空白对照组且具有统计学意义(P<0.05);ALP染色实验结果显示6 h组、12 h组中ALP活性明显高于空白对照组;茜素红染色实验结果显示6 h组、12 h组骨钙结节形成明显高于空白对照组。结论周期性牵张力的施加会促进hPDLFs中BMP-2的表达并促进hPDLFs成骨分化,可为临床口腔正畸进一步提供理论依据。 展开更多
关键词 周期性牵张力 人牙周膜成纤维细胞 骨形态发生蛋白-2 成骨分化
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Both Atorvastatin and Probucol Can Down-regulate BMP-2 Expression Induced by Oxidized Low Density Lipoprotein in Human Umbilical Vein Cells
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作者 Ming Zhang, Shenghua Zhou, Liping Liu, Xuping Li, XiangQian ShenDepartment of Cardiology, The Second Xiangya Hospital, Central South University, Changsha 410011, China 《South China Journal of Cardiology》 CAS 2007年第3期152-157,共6页
Objectives Bone morphogenetic protein-2 (BMP-2) plays an key role both in vascular development and pathophysiological processes. However, the mechanisms of oxidized low density lipoprotein (ox-LDL) and combinated with... Objectives Bone morphogenetic protein-2 (BMP-2) plays an key role both in vascular development and pathophysiological processes. However, the mechanisms of oxidized low density lipoprotein (ox-LDL) and combinated with atorvastatin or probucol on BMP-2 expression are entirely unknown in human umbilical vein cells. Methods The HUVECs were treated by ox-LDL and combinated with atorvastatin, probucol. The expression of BMP-2, NF-κB65, PPARγ mRNA was examined by RT-PCR analysis and ELISA method. The MDA and SOD were detected by routine methods. Results Ox-LDL can induced BMP-2 mNRA expression, associated with NF-κB65 mNRA expression activation. Both atorvastatin and probucol can suppress BMP-2 and NF-κB65 expression induced by oxLDL and upregulate the expression of PPARγ. Furthermore, the increase of supernatant MDA levels and decrease of supernatant SOD levels resulted from oxLDL treatment can be reversed by probucol or atorvastatin. Conclusions OxLDL-induced BMP-2 mNRA expression can be suppressed by atorvastatin and probucol, which may be accomplished by activating NF-κB65 expression and upregulating the expression of PPARγ. Our findings also indicate that that BMP-2 mNRA expression includes the activation of reactive oxygen species. 展开更多
关键词 bone morphogenetic protein-2 (bmp-2) OX-LDL ATORVASTATIN PROBUCOL
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Osteogenesis Capacity of a Novel BMP/α-TCP Bioactive Composite Bone Cement
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作者 杨为中 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2004年第2期30-34,共0页
To improve the osteogenesis ability of a-tricalcium phosphate (α-TCP) bone cement, a novel BMP/ α-TCP composite bone cement was prepared. By measuring the setting time and compressive strength, the hydration charact... To improve the osteogenesis ability of a-tricalcium phosphate (α-TCP) bone cement, a novel BMP/ α-TCP composite bone cement was prepared. By measuring the setting time and compressive strength, the hydration characteristic of bone cement was evaluated. Animal experiments including histological observation, radiographic investigation as well as digital image analyses reveal the difference of osteogenesis ability among BMP,a-TCP bone cement and BMP/α-TCP composite bone cement. Results show that α-TCP bone cement possesses excellent hydration and setting properties as well as high mechanical property. Comparison experiments show that BMP/ α-TCP composite bone cement has a stronger osteogenesis ability. The gross observation of the implant site does not exhibit any inflammation or necrosis. Histological analyses reveal that the material has good osteointegration with host bone, and new bone formation is detected within the materials, which are degrading. Strong osteogenesis ability of the composite is due to not only the excellent osteoconductive potential but also the osteoinductive potential contributed by active BMP releasing and the material degradation. Large skull defect could be well-healed by filling BMP/α-TCP composite bone cement. This novel material proves itself to be an absorbable and bioactive bone cement with an osteogenesis ability. Key words α-tricalcium phosphate (α-TCP) - bone morphogenetic proteins (BMP) - bone cement - osteogenesis - osteoinductivity - bone tissue engineering Funded by 863 Hi-Tech Research and Development Program of China (2002AA326080) and the Fund for Outstanding Young Teacher of the Education Ministry of China(2002123) 展开更多
关键词 α-tricalcium phosphate (α-TCP) bone morphogenetic proteins (bmp) bone cement OSTEOGENESIS OSTEOINDUCTIVITY bone tissue engineering
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磷酸钙/BMP复合生物活性骨水泥水化性能及诱导成骨特性研究 被引量:10
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作者 杨为中 周大利 +2 位作者 尹光福 尹绍雅 郑昌琼 《无机材料学报》 SCIE EI CAS CSCD 北大核心 2005年第5期1174-1180,共7页
采用微细α-磷酸三钙(α-TCP)粉料、辅助料与冻干牛骨形态发生蛋白(BMP)预先固相混合制备了新型磷酸钙(CPC)/BMP复合生物骨水泥.通过水化、凝固性能研究优化了配料成分、调和液和促凝剂组成;通过大鼠肌袋种植实验研究了骨水泥的异位成... 采用微细α-磷酸三钙(α-TCP)粉料、辅助料与冻干牛骨形态发生蛋白(BMP)预先固相混合制备了新型磷酸钙(CPC)/BMP复合生物骨水泥.通过水化、凝固性能研究优化了配料成分、调和液和促凝剂组成;通过大鼠肌袋种植实验研究了骨水泥的异位成骨性能.结果表明:以α-TCP:CaHPO4:CaO(0.95:0.025:0.025)为固相配料,以0.25mol/LNaH2PO4/Na2HPO4混合液([P]T=0.5mol/L)作为调合液可制备性能优异的骨水泥材料,骨水泥初凝时间为6min,终凝时间为30min,固化强度达33MPa,达到临床手术的要求;α-TCP粉料粒度对骨水泥凝固性能影响显著,实验选用α-TCP粉料粒径d50为1.3μm;骨水泥在Hank's溶液中浸泡5天抗压强度可达最大值;骨水泥块经浸泡后内部生成针状羟基磷灰石晶体的网状结构.新型CPC/BMP复合骨水泥异位成骨作用明显,4周即能快速形成板层骨结构,证明该新型复合材料具有较强的诱导成骨活性.该生物活性骨水泥复合材料可望成为一类新型组织工程骨修复材料. 展开更多
关键词 磷酸钙 骨水泥 水化性能 骨形态发生蛋白(bmp)
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胶原蛋白/BMP复合材料的制备和成骨性能研究 被引量:10
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作者 谈伟强 徐靖宏 +2 位作者 刘友山 林军 王慧明 《生物工程学报》 CAS CSCD 北大核心 2008年第2期272-277,共6页
以胶原膜(含87.5 mgⅠ型胶原蛋白)为载体,复合3.5 mg rhBMP-2(人基因重组骨形成蛋白-2),制备胶原蛋白/BMP复合材料。复合材料首先在兔背阔肌中埋置,预构新生骨组织,并采用ALP染色、Von Kossa染色和HE染色等观察复合材料的成骨过程和组... 以胶原膜(含87.5 mgⅠ型胶原蛋白)为载体,复合3.5 mg rhBMP-2(人基因重组骨形成蛋白-2),制备胶原蛋白/BMP复合材料。复合材料首先在兔背阔肌中埋置,预构新生骨组织,并采用ALP染色、Von Kossa染色和HE染色等观察复合材料的成骨过程和组织形态。然后将形成的新骨组织游离移植修复自体下颌骨体部洞穿性缺损;并设以胶原为载体的rhBMP-2复合骨修复材料直接修复为对照组,骨缺损不修复组为空白组。采用X线、抗压强度、硬组织切片、四环素荧光染色、骨形态计量检查,观察复合材料修复骨缺损的质量和效果。结果表明,胶原蛋白/BMP复合材料在兔背阔肌中4~6周成骨,胶原材料于3~5周降解;成骨过程为是以软骨成骨为主的方式,新骨形态为编织骨,可见明显的微血管分布;游离移植修复自体下颌骨缺损,6周缺损区为骨性愈合,与对照组在抗压强度(P=0.041)、新骨量(P= 0.034)均有显著性差异。胶原蛋白/BMP复合材料在骨骼肌中形成的新生骨组织可作为供骨修复一定范围的骨缺损。 展开更多
关键词 胶原蛋白 骨形态发生蛋白-2 异位成骨 骨缺损
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转染BMP-2基因的兔BMSCs种植PLA/PCL支架体外构建组织工程骨 被引量:12
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作者 卜丽莎 李建军 +5 位作者 高申 杨绍娟 张文岚 崔亚南 刘建国 徐莘香 《中国矫形外科杂志》 CAS CSCD 2004年第9期677-679,共3页
目的 :用腺病毒载体将人骨形态发生蛋白 2 (BMP 2 )基因导入兔骨髓基质干细胞 (BMSCs) ,种植PLA/PCL (聚乳酸 /聚己内酯 )支架体外构建组织工程骨。方法 :蛋白印迹法检测转染后细胞BMP 2的表达 ,流式细胞仪和ALP活性检测分析基因转染... 目的 :用腺病毒载体将人骨形态发生蛋白 2 (BMP 2 )基因导入兔骨髓基质干细胞 (BMSCs) ,种植PLA/PCL (聚乳酸 /聚己内酯 )支架体外构建组织工程骨。方法 :蛋白印迹法检测转染后细胞BMP 2的表达 ,流式细胞仪和ALP活性检测分析基因转染对细胞增殖分化的影响。然后将转染后细胞接种到PLA/PCL支架上 ,扫描电镜观察细胞贴附、生长状况。结果 :转染后 ,BMSCs表达BMP 2 ,S期细胞比例和ALP活性明显增高。扫描电镜见转染细胞分布均匀 ,伸展良好。结论 :BMP 2基因转染BMSCs ,可促进细胞增殖分化。转染后细胞在PLA/PCL支架上生长良好 ,BMP 2基因治疗的组织工程骨构建成功。 展开更多
关键词 腺病毒 骨形态发生蛋白 骨髓基质干细胞 组织工程骨
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NNB/BMP复合物在胫骨骨折不愈合治疗中的应用 被引量:13
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作者 陈光 杨建中 +4 位作者 许会敏 王铭 曹文宏 朱勇 王美婷 《中国矫形外科杂志》 CAS CSCD 2000年第8期758-761,共4页
目的 :验证天然型无机骨 (NNB)与骨形态形成蛋白 (BMP)复合物取代自体松质骨移植治疗胫骨陈旧性骨折及骨折不愈合的可行性。方法 :将NNB与BMP按 2 0∶1的比例 ,经化学方法制成NNB/BMP复合物 ,以 80例陈旧胫骨骨折及骨折不愈合为研究对... 目的 :验证天然型无机骨 (NNB)与骨形态形成蛋白 (BMP)复合物取代自体松质骨移植治疗胫骨陈旧性骨折及骨折不愈合的可行性。方法 :将NNB与BMP按 2 0∶1的比例 ,经化学方法制成NNB/BMP复合物 ,以 80例陈旧胫骨骨折及骨折不愈合为研究对象 ,本次手术到受伤时间平均 8.5个月 ( 4~ 2 4个月 ) ,平均年龄 3 5岁 ,随机分为对照组 3 0例 ,治疗组 5 0例 ,依治疗方法不同随机分为甲、乙二组 ,分别在骨折区域植入自体松质骨与NNB/BMP ,观察术后全身及切口局部反应及连续X线片表现。随访平均 19个月 ( 13~ 3 6个月 )。按Johner Wruh评分评价最终效果。结果 :治疗组与对照组愈合时间上有显著差异 ;治疗组甲与治疗组乙间无显著差异。结论 :NNB/BMP复合物具有与自体松质骨相同的促进骨愈合的生物学活性 ,是一种理想的促进骨折愈合的松质骨替代材料。 展开更多
关键词 骨形态形成蛋白 胫骨骨折 骨折不愈合 NNB 治疗
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补肾活血方对骨质疏松症模型大鼠骨密度及BMP-2蛋白表达影响实验研究 被引量:10
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作者 姜坤 周强 +4 位作者 尚德阳 孙鑫 邢克欣 林庶茹 邓洋洋 《辽宁中医药大学学报》 CAS 2016年第5期20-22,共3页
目的:通过观察摘除卵巢复制骨质疏松症模型大鼠股骨中BMP-2蛋白表达变化,探究补肾活血方能有效治疗质疏松症的机理。方法:实验采用摘除大鼠双侧卵巢的方法复制骨质疏松症模型,并将大鼠分为正常组、假手术组、模型组、补肾活血方组、阳... 目的:通过观察摘除卵巢复制骨质疏松症模型大鼠股骨中BMP-2蛋白表达变化,探究补肾活血方能有效治疗质疏松症的机理。方法:实验采用摘除大鼠双侧卵巢的方法复制骨质疏松症模型,并将大鼠分为正常组、假手术组、模型组、补肾活血方组、阳性药物福善美组和骨疏康组,术后2 d开始给药干预,6次/周,持续12周后检测各组大鼠股骨中BMP-2蛋白表达水平及骨密度。结果:补肾活血组和骨疏康组、福善美组与模型组相比较,股骨密度都得到了一定提高(P<0.01);BMP-2蛋白表达水平也都有显著升高(P<0.01)。结论:补肾活血方对骨质疏松症模型大鼠有明显的治疗作用,其作用机制可能是在一定程度上提高了股骨组织中BMP-2的水平,进而抑制骨组织的吸收,促进骨组织的形成。 展开更多
关键词 补肾活血方 骨质疏松症 骨密度 bmp-2蛋白
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以纤维蛋白胶为载体复合BMP和bFGF的注射型骨修复材料诱导异位成骨的实验研究 被引量:17
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作者 雷伟 崔赓 +7 位作者 胡蕴玉 李洁 吕荣 丛锐 靳小兵 汪培铭 杜俊杰 吕昌伟 《中国矫形外科杂志》 CAS CSCD 2004年第10期765-767,共3页
目的 :了解以纤维蛋白胶 (Fibrinsealant,FS )为载体的注射型骨修复材料异位诱导成骨的作用 ,为其临床的应用提供实验依据。方法 :实验分组为 :实验组b (FS +bFGF+bBMP)、对照组b1(FS +bBMP)、对照组b2 (bBMP)、实验组r (FS +bFGF +rhBM... 目的 :了解以纤维蛋白胶 (Fibrinsealant,FS )为载体的注射型骨修复材料异位诱导成骨的作用 ,为其临床的应用提供实验依据。方法 :实验分组为 :实验组b (FS +bFGF+bBMP)、对照组b1(FS +bBMP)、对照组b2 (bBMP)、实验组r (FS +bFGF +rhBMP 2 )、对照组r1(FS +rhBMP)、对照组r2 (rhBMP)、对照组FS及空白对照组。将各组材料注射或植入小鼠肌袋内 ,采用放射学、形态学、碱性磷酸酶 (ALP)检测等方法对其成骨效应进行研究。结果 :在以bBMP为成骨因子的实验区中 ,实验组b具有高效的骨诱导活性 ,其成骨量显著高于对照组b1、对照组b2、对照组FS及空白对照组(P <0 .0 1) ;在以rhBMP 2为成骨因子的实验区中 ,实验组r同样具有高效的骨诱导活性 ,其成骨量也显著高于对照组r1、对照组r2、对照组FS及空白对照组 (P <0 .0 1)。结论 :以FS为载体复合BMP和bFGF的注射型骨修复材料具有高效的骨诱导活性 ,bFGF可明显增强BMP的骨诱导活性。 展开更多
关键词 纤维蛋白胶 骨形态发生蛋白 碱性成纤维细胞生长因子 载体 小鼠
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人骨形态发生蛋白2(BMP-2)在巴斯德毕赤酵母中的表达 被引量:4
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作者 梁东春 左爱军 +2 位作者 王宝利 郭刚 张镜宇 《中国生物工程杂志》 CAS CSCD 北大核心 2005年第7期61-65,共5页
以重组质粒pUC-BMP2为模板PCR扩增人BMP-2成熟肽编码序列,将该序列克隆入pGEM-T载体进行DNA序列分析后亚克隆入分泌型毕赤酵母表达载体pPIC9K中。重组质粒oPIC9K-BMP2经BelⅡ酶切后回收线性化片段,聚乙二醇法转染毕赤酵母茵株GS115。PC... 以重组质粒pUC-BMP2为模板PCR扩增人BMP-2成熟肽编码序列,将该序列克隆入pGEM-T载体进行DNA序列分析后亚克隆入分泌型毕赤酵母表达载体pPIC9K中。重组质粒oPIC9K-BMP2经BelⅡ酶切后回收线性化片段,聚乙二醇法转染毕赤酵母茵株GS115。PCR筛选整合有人BMP-2基因的酵母细胞重组子,以甲醇进行诱导表达,于酵母细胞培养基中可检测到rhBMP-2。体外培养条件下,所得rhBMP-2可增加2T3小鼠成骨细胞内碱性磷酸酶活性;体内实验.rhBMP-2冻干粉可于小鼠骨四头肌肌袋中诱导软骨细胞群产生。 展开更多
关键词 巴斯德毕赤酵母 骨形态发生蛋白 rhbmp-2 DNA序列分析 碱性磷酸酶活性 酵母表达载体 体外培养条件 重组质粒 PCR扩增 聚乙二醇法 细胞培养基 编码序列 酵母菌株 酵母细胞 诱导表达 体内实验 成熟肽 PUC 分泌型 亚克隆 T载体
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