Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human...Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells展开更多
Background Bone morphogenetic protein （BMP）-2, alkaline phosphatase （ALP）, and collagen type I are known to play a critical role in the process of bone remodeling. However, the relationship between mechanical stra...Background Bone morphogenetic protein （BMP）-2, alkaline phosphatase （ALP）, and collagen type I are known to play a critical role in the process of bone remodeling. However, the relationship between mechanical strain and the expression of BMP-2, ALP, and COL-I in osteoblasts was still unknown. The purpose of this study was to investigate the effects of different magnitudes of mechanical strain on osteoblast morphology and on the expression of BMP-2, ALP, and COL-I. Methods Osteoblast-like cells were flexed at four deformation rates （0, 6%, 12%, and 18% elongation）. The expression of BMP-2 mRNA, ALP, and COL-I in osteoblast-like cells were determined by real-time quantitative reverse transcription polymerase chain reaction, respectively. The results were subjected to analysis of variance （ANOVA） using SPSS 13.0 statistical software. Results The cells changed to fusiform and grew in the direction of the applied strain after the mechanical strain was loaded. Expression level of the BMP-2, ALP, and COL-I increased magnitude-dependently with mechanical loading in the experimental groups, and the 12% elongation group had the highest expression （P 〈0.05）. Conclusion Mechanical strain can induce morphological change and a magnitude-dependent increase in the expression of BMP-2, ALP, and COL-I mRNA in osteoblast-like cells, which might influence bone remodeling in orthodontic treatment.展开更多
Alkaline phosphatase(ALP)flare has been reported to occur during cancer treatment as a favorable event,particularly in the presence of bone metastasis.There have been only a few reports in lung cancer and associated r...Alkaline phosphatase(ALP)flare has been reported to occur during cancer treatment as a favorable event,particularly in the presence of bone metastasis.There have been only a few reports in lung cancer and associated radiographic findings have seldom been described.The authors observed ALP flare in a female patient with lung adenocarcinoma soon after the initiation of gefitinib.Moreover,on computed tomography,metastatic lesions of the rib and thoracic spine showed marked hyperostosis,with sizes larger than the original bone structure,suggesting efficacy of gefitinib.The significance of such hyperostosis should be elucidated.展开更多
目的利用Meta分析的方法,研究铅暴露对人群骨代谢的影响。方法通过对中国期刊全文数据库、万方数据库、维普中文科技期刊数据库、PubMed、Web of science五个数据库,检索建库至2022年3月公开发表的铅暴露对人群骨代谢相关指标影响的文献...目的利用Meta分析的方法,研究铅暴露对人群骨代谢的影响。方法通过对中国期刊全文数据库、万方数据库、维普中文科技期刊数据库、PubMed、Web of science五个数据库,检索建库至2022年3月公开发表的铅暴露对人群骨代谢相关指标影响的文献,对文献进行筛选和资料提取。以AHRQ横断面研究评价标准对纳入文献进行质量评价。维生素D3、骨钙素、骨碱性磷酸酶作为骨代谢指标进行分析。采用Stata16、Review Manager 5.3软件进行森林图绘制、敏感性分析及发表偏倚检验,标准化均数差(SMD)及其95%可信区间(95%CI)作为效应指标。结果共纳入13篇文献(n=2029)。纳入的研究存在中高度异质性(I^(2)>50%),采取随机效应模型。相对于非铅接触人群,铅接触人群的钙磷代谢调节指标维生素D3的SMD为-1.11(95%CI:-2.20~-0.02),骨钙素的SMD为-0.50(95%CI:-0.91~-0.09),骨碱性磷酸酶的SMD为0.80(95%CI:0.42~1.19),差异均具有统计学意义(P<0.01)。结论Meta分析结果表明铅暴露影响人群的骨代谢,其中骨碱性磷酸酶水平升高,维生素D3、骨钙素水平下降。展开更多
文摘Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells
文摘Background Bone morphogenetic protein （BMP）-2, alkaline phosphatase （ALP）, and collagen type I are known to play a critical role in the process of bone remodeling. However, the relationship between mechanical strain and the expression of BMP-2, ALP, and COL-I in osteoblasts was still unknown. The purpose of this study was to investigate the effects of different magnitudes of mechanical strain on osteoblast morphology and on the expression of BMP-2, ALP, and COL-I. Methods Osteoblast-like cells were flexed at four deformation rates （0, 6%, 12%, and 18% elongation）. The expression of BMP-2 mRNA, ALP, and COL-I in osteoblast-like cells were determined by real-time quantitative reverse transcription polymerase chain reaction, respectively. The results were subjected to analysis of variance （ANOVA） using SPSS 13.0 statistical software. Results The cells changed to fusiform and grew in the direction of the applied strain after the mechanical strain was loaded. Expression level of the BMP-2, ALP, and COL-I increased magnitude-dependently with mechanical loading in the experimental groups, and the 12% elongation group had the highest expression （P 〈0.05）. Conclusion Mechanical strain can induce morphological change and a magnitude-dependent increase in the expression of BMP-2, ALP, and COL-I mRNA in osteoblast-like cells, which might influence bone remodeling in orthodontic treatment.
文摘Alkaline phosphatase(ALP)flare has been reported to occur during cancer treatment as a favorable event,particularly in the presence of bone metastasis.There have been only a few reports in lung cancer and associated radiographic findings have seldom been described.The authors observed ALP flare in a female patient with lung adenocarcinoma soon after the initiation of gefitinib.Moreover,on computed tomography,metastatic lesions of the rib and thoracic spine showed marked hyperostosis,with sizes larger than the original bone structure,suggesting efficacy of gefitinib.The significance of such hyperostosis should be elucidated.
文摘目的利用Meta分析的方法,研究铅暴露对人群骨代谢的影响。方法通过对中国期刊全文数据库、万方数据库、维普中文科技期刊数据库、PubMed、Web of science五个数据库,检索建库至2022年3月公开发表的铅暴露对人群骨代谢相关指标影响的文献,对文献进行筛选和资料提取。以AHRQ横断面研究评价标准对纳入文献进行质量评价。维生素D3、骨钙素、骨碱性磷酸酶作为骨代谢指标进行分析。采用Stata16、Review Manager 5.3软件进行森林图绘制、敏感性分析及发表偏倚检验,标准化均数差(SMD)及其95%可信区间(95%CI)作为效应指标。结果共纳入13篇文献(n=2029)。纳入的研究存在中高度异质性(I^(2)>50%),采取随机效应模型。相对于非铅接触人群,铅接触人群的钙磷代谢调节指标维生素D3的SMD为-1.11(95%CI:-2.20~-0.02),骨钙素的SMD为-0.50(95%CI:-0.91~-0.09),骨碱性磷酸酶的SMD为0.80(95%CI:0.42~1.19),差异均具有统计学意义(P<0.01)。结论Meta分析结果表明铅暴露影响人群的骨代谢,其中骨碱性磷酸酶水平升高,维生素D3、骨钙素水平下降。
