On June 25,1990,seven technicians from a radiation medicine department in Shanghai were accidently exposed to a50 Co gamma radiation source within a short duration at a large dose rate,causing 7 cases of bone marrow f...On June 25,1990,seven technicians from a radiation medicine department in Shanghai were accidently exposed to a50 Co gamma radiation source within a short duration at a large dose rate,causing 7 cases of bone marrow form of acute radiation sickness(ARS).This unfortunate large dose of irradiation incidence is called '6.25 radiation accident'.This article reports the clinical progress of this relatively homogenous external irradiation, doses,early management and therapeutic outcomes of the 7 cases of bone marrow form of ARS. Two cases of extremely severe degree bone marrow form of ARS received about ll￣12 Gy total body irradiation (TBI). The living duration of both patients was prolonged owing to semi-identical HLA of allogenous bone marrow transplantation (BMT). One of the patients lived 25 d and the other survived 9o d. The donor chromosome XX showed that BMT was fully engrafted and hematopoiesis was reconstructed in 'Wan'. The remaining 5 cases of severe and moderate degree bone marrow form of ARS received 5. 2 Gy,4.1 Gy,2.5 Gy,2.4 Gy and 2.0 Gy irradiation. As a result of appropriate treatment,these patients passed smoothly the four stages of the clinical course without macro-symptoms and signs at the critical stages, presented with no significant infection and hemorrhagic syndrome. No similar report has been presented in our country and other countries.The 5 cases of ARS were discharged from the hospital because of clinical convalescence.Differential therapeutic management including protective isolation is recommended,and the effect of antiradiation agents such as Estradiol benzoate and Chinese herbal medicines is evaluated. Selective use of antiinfectious antibiotics, whole blood and blood component transfusion,gamma globulin and fetal liver cell infusion when indicated are also discussed.Sorted out by Professor Mai Zhiguang (Division of Radiation Medicine, Second Military Medical University,Shanghai,200433)展开更多
Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed col...Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.展开更多
文摘On June 25,1990,seven technicians from a radiation medicine department in Shanghai were accidently exposed to a50 Co gamma radiation source within a short duration at a large dose rate,causing 7 cases of bone marrow form of acute radiation sickness(ARS).This unfortunate large dose of irradiation incidence is called '6.25 radiation accident'.This article reports the clinical progress of this relatively homogenous external irradiation, doses,early management and therapeutic outcomes of the 7 cases of bone marrow form of ARS. Two cases of extremely severe degree bone marrow form of ARS received about ll￣12 Gy total body irradiation (TBI). The living duration of both patients was prolonged owing to semi-identical HLA of allogenous bone marrow transplantation (BMT). One of the patients lived 25 d and the other survived 9o d. The donor chromosome XX showed that BMT was fully engrafted and hematopoiesis was reconstructed in 'Wan'. The remaining 5 cases of severe and moderate degree bone marrow form of ARS received 5. 2 Gy,4.1 Gy,2.5 Gy,2.4 Gy and 2.0 Gy irradiation. As a result of appropriate treatment,these patients passed smoothly the four stages of the clinical course without macro-symptoms and signs at the critical stages, presented with no significant infection and hemorrhagic syndrome. No similar report has been presented in our country and other countries.The 5 cases of ARS were discharged from the hospital because of clinical convalescence.Differential therapeutic management including protective isolation is recommended,and the effect of antiradiation agents such as Estradiol benzoate and Chinese herbal medicines is evaluated. Selective use of antiinfectious antibiotics, whole blood and blood component transfusion,gamma globulin and fetal liver cell infusion when indicated are also discussed.Sorted out by Professor Mai Zhiguang (Division of Radiation Medicine, Second Military Medical University,Shanghai,200433)
基金supported by the National Natural Science Foundation of China,No.30471836
文摘Non-adherent bone marrow cell-derived mesenchymal stem cells from C57BL/6J mice were sepa- rated and cultured using the ＂pour-off＂ method. Non-adherent bone marrow cell-derived mesen- chymal stem ceils developed colony-forming unit-fibroblasts, and could be expanded by supple- mentation with epidermal growth factor. Immunocytochemistry showed that the non-adherent bone marrow cell-derived mesenchymal stem cells exposed to basic fibroblast growth factor/epidermal growth factor/nerve growth factor expressed the neuron specific markers, neurofilament-200 and NeuN, in vitro. Non-adherent bone marrow cell-derived mesenchymal stem cells from 13-galactosidase transgenic mice were also transplanted into focal ischemic brain （right corpus striatum） of C57BL/6J mice. At 8 weeks, cells positive for LacZ and 13-galactosidase staining were observed in the ischemic tissues, and cells co-labeled with both 13-galactosidase and NeuN were seen by double immunohistochemical staining. These findings suggest that the non-adherent bone marrow cell-derived mesenchymal stem cells could differentiate into neuronal-like cells in vitro and in vivo.
