Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the rec...Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective effect on cerebral ischemia/reperfusion injury in rats.展开更多
Objective:To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia（AA）mice.Methods:The model of immune aplastic anemia mice was in duced by means of 6.0...Objective:To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia（AA）mice.Methods:The model of immune aplastic anemia mice was in duced by means of 6.0GY60γ-rayirr adi at ion and lym phocyte infusion through tail vein.Immuneinduce dAA micewere divided into 3 groups:thenormal group,the AA control group and the ligustrazine group.Miceof the ligustrazine group were fed by 4 mg of ligustrazine in jecti on twicea day bygastro gavage.On the 10 thday,CD34 an tigen expression intensity of bone marrow cell membrane was measured by flowcy tometer an alysis system.Results:CD34 antigen expressionin tensity of ligustrazine group was 77.6±6.5,with no statistic difference from that in normal group（80.0±2.6),while that of the control group was muchhigher（68.6±4.5,P＜0.05).Conclusion:Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through in fluencing on bone marrowmicro-environment so as to increase the CD34antigen expression of bone marrow cells.展开更多
BACKGROUND: Asthma is clinically related with the degree of eosinophilic inflammation. How asthmatic airway inflammation is affected is still poorly understood. So the effects of bone marrow-derived hematopoietic cell...BACKGROUND: Asthma is clinically related with the degree of eosinophilic inflammation. How asthmatic airway inflammation is affected is still poorly understood. So the effects of bone marrow-derived hematopoietic cells expressing CD(34) (CD(34)(+)) and interleukin-5 (IL-5) receptor messenger RNA (IL-5R mRNA+) on asthmatic airway inflammation were investigated. METHODS: Balb/c mice were sensitized and challenged by ovalbumin (OVA) to establish an asthmatic model while control mice were sensitized and exposed to sterile saline. The mice were killed at different time points after being challenged by OVA and sterile saline. Then, bronchoalveolar lavage fluid (BALF), peripheral blood (PB) and bone marrow (BM) were prepared. Eosinophils in PB (PBEOS) and BALF (BALFEOS), nuclear cells in BALF, PB and BM were counted. By flow cytometry, the percentage of CD(34)(+) cells to nucleated cells in PB, BM and the relative number of CD(34)(+) cells in PB (PBCD(34)(+)) and BM (BMCD(34)(+)) were calculated. Immunocytochemistry and in situ hybridization were used to investigate the hematopoietic cells with co-localized expression of CD(34) and IL-5R mRNA in BM (BMCD34+IL-5R mRNA+). The percentage of BMCD34+IL-5R mRNA+ to BMCD(34)(+) was calculated. RESULTS: Twelve hours after challenge by OVA, BALFEOS and PBEOS in the experimental group were significantly higher than those in the control group (P展开更多
基金supported by a grant from the State"Major New Drug Creation"Science and Technology Major Project in China,No.2009ZX09103-324a grant from the Henan Provincial Science and Technology Innovation Team in University in China,No.2012IRTSTHN011
文摘Previous studies have shown that Radix Ilicis Pubescentis total flavonoids have a neuroprotective effect, but it remains unclear whether Radix Ilicis Pubescentis total flavonoids have a synergistic effect with the recombinant human granulocyte colony stimulating factor-mobilized bone marrow stem cell transplantation on cerebral ischemia/reperfusion injury. Rat ischemia models were administered 0.3, 0.15 and 0.075 g/kg Radix Ilicis Pubescentis total flavonoids from 3 days before modeling to 2 days after injury. Results showed that Radix Ilicis Pubescentis total flavonoids could reduce pathological injury in rats with cerebral ischemia/reperfusion injury. The number of Nissl bodies increased, Bax protein expression decreased, Bcl-2 protein expression increased and the number of CD34-positive cells increased. Therefore, Radix Ilicis Pubescentis total flavonoids can improve the bone marrow stem cell mobilization effect, enhance the anti-apoptotic ability of nerve cells, and have a neuroprotective effect on cerebral ischemia/reperfusion injury in rats.
文摘Objective:To explore the effect of ligustrazine on CD34 antigen expression of bone marrow cells in immune-induced aplastic anemia（AA）mice.Methods:The model of immune aplastic anemia mice was in duced by means of 6.0GY60γ-rayirr adi at ion and lym phocyte infusion through tail vein.Immuneinduce dAA micewere divided into 3 groups:thenormal group,the AA control group and the ligustrazine group.Miceof the ligustrazine group were fed by 4 mg of ligustrazine in jecti on twicea day bygastro gavage.On the 10 thday,CD34 an tigen expression intensity of bone marrow cell membrane was measured by flowcy tometer an alysis system.Results:CD34 antigen expressionin tensity of ligustrazine group was 77.6±6.5,with no statistic difference from that in normal group（80.0±2.6),while that of the control group was muchhigher（68.6±4.5,P＜0.05).Conclusion:Ligustrazine could promote proliferation of stem and progenitor cell of AA mice through in fluencing on bone marrowmicro-environment so as to increase the CD34antigen expression of bone marrow cells.
基金ThisprojectwassupportedbytheNationalNaturalScienceFoundationofChina (No 3 9770 3 40 )
文摘BACKGROUND: Asthma is clinically related with the degree of eosinophilic inflammation. How asthmatic airway inflammation is affected is still poorly understood. So the effects of bone marrow-derived hematopoietic cells expressing CD(34) (CD(34)(+)) and interleukin-5 (IL-5) receptor messenger RNA (IL-5R mRNA+) on asthmatic airway inflammation were investigated. METHODS: Balb/c mice were sensitized and challenged by ovalbumin (OVA) to establish an asthmatic model while control mice were sensitized and exposed to sterile saline. The mice were killed at different time points after being challenged by OVA and sterile saline. Then, bronchoalveolar lavage fluid (BALF), peripheral blood (PB) and bone marrow (BM) were prepared. Eosinophils in PB (PBEOS) and BALF (BALFEOS), nuclear cells in BALF, PB and BM were counted. By flow cytometry, the percentage of CD(34)(+) cells to nucleated cells in PB, BM and the relative number of CD(34)(+) cells in PB (PBCD(34)(+)) and BM (BMCD(34)(+)) were calculated. Immunocytochemistry and in situ hybridization were used to investigate the hematopoietic cells with co-localized expression of CD(34) and IL-5R mRNA in BM (BMCD34+IL-5R mRNA+). The percentage of BMCD34+IL-5R mRNA+ to BMCD(34)(+) was calculated. RESULTS: Twelve hours after challenge by OVA, BALFEOS and PBEOS in the experimental group were significantly higher than those in the control group (P
