Grain size and weight are closely related traits determining yield in rice(Oryza sativa L.).Since indica and japonica rice varieties differ significantly in multiple traits,a high-generation recombinant inbred line(RI...Grain size and weight are closely related traits determining yield in rice(Oryza sativa L.).Since indica and japonica rice varieties differ significantly in multiple traits,a high-generation recombinant inbred line(RIL)population derived from the crossing LH9(indica)and RPY(japonica)was used to map grainrelated traits in six environments.Pyramiding of the quantitative trait loci(QTL)for thousand-grain weight showed that combinations of multiple QTL significantly increased the phenotypic effect.A novel gene named GSW3.1 controlling grain size and weight was discovered using the major QTL for the colocalization of grain width and thousand-grain weight on chromosome 3.Gene editing revealed that GSW3.1(LOC_Os03g16850)was pleiotropic,positively regulating grain size and weight while affecting several other agronomic traits.Haplotype analysis indicated that some traits,including grain width and weight,were highly correlated with indica-japonica differentiation.展开更多
Objective: To evaluate psychometric properties of a Chinese version of Adult Attachment Questionnaire (AAQ3.1). Methods: Score s on the AAQ3.1 were c ompared among 152 students,114 students’ parents and 37 patients. ...Objective: To evaluate psychometric properties of a Chinese version of Adult Attachment Questionnaire (AAQ3.1). Methods: Score s on the AAQ3.1 were c ompared among 152 students,114 students’ parents and 37 patients. Results: In ternal consistency in terms of item-total correlations was found to be high, but somewhat lower than what was found in the West. High discriminative power was f ound for differentiating between clinical and non-clinical samples, as well as b etween adult and adolescent samples. Significant correlation was found between husbands and wives. Conclusion: AAQ3.1 have acceptable re liability and validity for evaluating attachment of Chinese adults.展开更多
目的:构建miR-21的真核表达载体,并使其在骨肉瘤MG63细胞中表达,为研究miR-21对骨肉瘤细胞MG63的作用打下基础。方法:根据miRNA的成熟序列以及附近约200多碱基共433个碱基序列,设计PCR引物,PCR扩增,退火后用T4连接到线性化的pcDNA3.1(+...目的:构建miR-21的真核表达载体,并使其在骨肉瘤MG63细胞中表达,为研究miR-21对骨肉瘤细胞MG63的作用打下基础。方法:根据miRNA的成熟序列以及附近约200多碱基共433个碱基序列,设计PCR引物,PCR扩增,退火后用T4连接到线性化的pcDNA3.1(+)质粒中,并对重组质粒进行双酶切、菌落PCR及测序分析,鉴定完全正确的重组质粒转染骨肉瘤细胞MG63,G418(400 mg/L)筛选4周获得miR-21稳定表达细胞系,用Northern blot及real time RT-RCR法鉴定pcDNA3.1(+)-miR-21可在真核细胞中过表达。结果:成功构建了miR-21的真核表达载体,并获得稳定转染重组质粒的细胞系。结论:miR-21的真核表达载体在骨肉瘤细胞MG63中稳定高表达,为进一步研究miR-21在骨肉瘤MG63中的功能及基因调控机制奠定了实验基础。展开更多
基金supported by the National Key Research and Development Program of China(2016YFD0100400)the National Special Key Project for Transgenic Breeding(2016ZX08001001)。
文摘Grain size and weight are closely related traits determining yield in rice(Oryza sativa L.).Since indica and japonica rice varieties differ significantly in multiple traits,a high-generation recombinant inbred line(RIL)population derived from the crossing LH9(indica)and RPY(japonica)was used to map grainrelated traits in six environments.Pyramiding of the quantitative trait loci(QTL)for thousand-grain weight showed that combinations of multiple QTL significantly increased the phenotypic effect.A novel gene named GSW3.1 controlling grain size and weight was discovered using the major QTL for the colocalization of grain width and thousand-grain weight on chromosome 3.Gene editing revealed that GSW3.1(LOC_Os03g16850)was pleiotropic,positively regulating grain size and weight while affecting several other agronomic traits.Haplotype analysis indicated that some traits,including grain width and weight,were highly correlated with indica-japonica differentiation.
文摘Objective: To evaluate psychometric properties of a Chinese version of Adult Attachment Questionnaire (AAQ3.1). Methods: Score s on the AAQ3.1 were c ompared among 152 students,114 students’ parents and 37 patients. Results: In ternal consistency in terms of item-total correlations was found to be high, but somewhat lower than what was found in the West. High discriminative power was f ound for differentiating between clinical and non-clinical samples, as well as b etween adult and adolescent samples. Significant correlation was found between husbands and wives. Conclusion: AAQ3.1 have acceptable re liability and validity for evaluating attachment of Chinese adults.
文摘目的:构建miR-21的真核表达载体,并使其在骨肉瘤MG63细胞中表达,为研究miR-21对骨肉瘤细胞MG63的作用打下基础。方法:根据miRNA的成熟序列以及附近约200多碱基共433个碱基序列,设计PCR引物,PCR扩增,退火后用T4连接到线性化的pcDNA3.1(+)质粒中,并对重组质粒进行双酶切、菌落PCR及测序分析,鉴定完全正确的重组质粒转染骨肉瘤细胞MG63,G418(400 mg/L)筛选4周获得miR-21稳定表达细胞系,用Northern blot及real time RT-RCR法鉴定pcDNA3.1(+)-miR-21可在真核细胞中过表达。结果:成功构建了miR-21的真核表达载体,并获得稳定转染重组质粒的细胞系。结论:miR-21的真核表达载体在骨肉瘤细胞MG63中稳定高表达,为进一步研究miR-21在骨肉瘤MG63中的功能及基因调控机制奠定了实验基础。
