First Serological Evidence of Borna Disease Virus in Healthy Horses from Yucatan, Mexico

Borna Disease Virus (BDV) causes a progressive non-suppurative meningoencephalitis that sometimes occurs in mortality;this disease has been reported for over two centuries ago in horses, sheep and cats in Central Europe and some regions of Asia. Currently, it is known that it causes neurological symptoms in various species of vertebrates including human beings. In Yucatan, Mexico, there is a single serological report about the circulation of BDV in schizophrenia patients;however, nothing is known about the circulation in animals. We obtained serum samples of 100 horses without apparent clinical signs caused by BDV infection, from various sites in the region. Antibodies against BDV were detected by electrochemiluminescence immunoassay (ECLIA) method with three recombinant proteins: BDV p24, BDV p40 and BDV p10 as antigens;obtaining a high seroprevalence of 44% (44/100). This study generates the first report of the probable activity of the BDV in healthy horses in Mexico and has expanded the infiltration area of BDV in the world. Nevertheless, several molecular investigations are required to detect BDV-RNA circulating and find sequences for clarification of the origin of BDV in Mexican horses.

Genome-wide profiling of long noncoding RNA expression patterns and CeRNA analysis in mouse cortical neurons infected with different strains of borna disease virus

Borna disease virus 1(BoDV-1)is neurotropic prototype of Bornaviruses causing neurological diseases and maintaining persistent infection in brain cells of mammalian species.Long non-coding RNA(lncRNA)is transcript of more than 200 nucleotides without proteincoding function regulating various biological processes as proliferation,apoptosis,cell migration and viral infection.However,regulatory of lncRNAs in BoDV-1 infection remains unknown.To identify differential expression profiles and predict functions of lncRNA in BoDV-1 infection,microarray data showed that 3528 lncRNAs and 2661 lncRNAs were differentially expressed in Strain V and Hu-H1 BoDV-infected groups compared with control groups,respectively.Gene Ontology(GO)and pathway analysis suggested that differential lncRNAs may be involved in regulation of metabolic,biological regulation,cellular process,endocytosis,viral infections and cell adhesion processes,cancer in both BoDV-infected strains.ENSMUST00000128469 was found down-regulated in both BoDV-infected groups compared with control groups consistent with microarray(p<0.05).ceRNA analysis indicated possible interaction networks as ENSMUST00000128469/miR-22-5p,miR-206-3p,miR-302b-5p,miR-302c-3p,miR-1a-3p/Igf1.Igf1 was found up-regulated in both BoDV-infected groups compared with control groups(p<0.05).Possible functions of predicted target mRNAs and miRNAs of ENSMUST00000128469 were involved in cell proliferation,transcriptional misregulation and proteoglycan pathways enriched in cancer.lncRNA may be involved in regulation of Hu-H1 inhibited cell proliferation and promoted apoptosis through NF-kB,JNK/MAPK signaling,BCL2 and CDK6/E2F1 pathways different from Strain V.Possible interaction networks as ENSMUST00000128469/miR-22-5p,miR-206-3p,miR-302b-5p,miR-302c-3p,miR-1a-3p/Igf1 may involve in regulation of cell proliferation,apoptosis,and cancer.