Bartonella henselae is a Gram-negative bacterium and is the causative agent of cat scratch disease.In humans,infections with B.henselae can result in acute or chronic systemic infections with diverse clinical symptoms...Bartonella henselae is a Gram-negative bacterium and is the causative agent of cat scratch disease.In humans,infections with B.henselae can result in acute or chronic systemic infections with diverse clinical symptoms.The current treatment for Bartonella infections with antibiotics such as doxycycline and rifampin is not always effective,presumably due in part to bacterial persistence.There are various anecdotal reports of herbal extracts used for treating patients with persistent Bartonella infections but their activity on B.henselae is unknown.In order to identify more effective therapies for persistent Bartonella infections,in this study,we tested the antimicrobial activity of botanical and herbal medicines and screened an herbal product collection against stationary phase B.henselae in vitro using a SYBR Green I/propidium iodide viability assay.We identified five herbal product extracts(at 1.67 mg/mL or 2.5mg/mL),including Cryptolepis sanguinolenta,Juglans nigra,Polygonum cuspidatum,Scutellaria baicalensis,and Scutellaria barbata,that had high activity against stationary phase B.henselae.Among them,C.sanguinolenta,J.nigra,and P.cuspidatum could eradicate all stationary phase B.henselae cells within 7days at 0.83 mg/mL or 1.25 mg/mL in drug exposure time-kill assays,whereas S.baicalensis and S.barbata showed relatively poor activity.These top hits were active against both stationary phase nongrowing B.henselae and log phase growing B.henselae in minimum inhibitory concentration testing.These findings may have implications for improved treatment of persistent Bartonella infections.展开更多
The rapid appraisal method was used to identity the botanicals used ethnomedicinally from a total of 300 randomly selected respondents drawn from the existing three geo-political zones of Ekiti State, Nigeria. The res...The rapid appraisal method was used to identity the botanicals used ethnomedicinally from a total of 300 randomly selected respondents drawn from the existing three geo-political zones of Ekiti State, Nigeria. The results obtained revealed that about 40% of the 71 botanicals identified presently rare. Most of the presently abundant botanicals are species primarily cultivated for other purpose other than medicine. Most of the identified species are valued for their curative effects on malaria and fever, the predominant diseases in the study area. The need for the conservation of the rare species cannot be over emphasised as most rural dwellers in the study area depend mostly on herbs from these species. Strategies towards the attainment of this goal are proposed.展开更多
The functions of medicinal botanical gardens are introduced firstly,and then the application status and planning principles of medicinal botanical gardens based on the theme of science popularization education are ana...The functions of medicinal botanical gardens are introduced firstly,and then the application status and planning principles of medicinal botanical gardens based on the theme of science popularization education are analyzed.Moreover,a typical application case is analyzed and summarized.Constructing a scientific medicinal botanical garden is conducive to increasing people's understanding of medicinal plants,and also meets the requirements of current national policies,thereby generating greater economic and social benefits.展开更多
基金supported by Bay Area Lyme Foundation,the Steven&Alexandra Cohen Foundation,LivLyme Foundation,and the Einstein-Sim Family Charitable Fund.The funders had no role in study design,data collection and interpretation,writing of the manuscript,or the decision to submit the manuscript for publication.
文摘Bartonella henselae is a Gram-negative bacterium and is the causative agent of cat scratch disease.In humans,infections with B.henselae can result in acute or chronic systemic infections with diverse clinical symptoms.The current treatment for Bartonella infections with antibiotics such as doxycycline and rifampin is not always effective,presumably due in part to bacterial persistence.There are various anecdotal reports of herbal extracts used for treating patients with persistent Bartonella infections but their activity on B.henselae is unknown.In order to identify more effective therapies for persistent Bartonella infections,in this study,we tested the antimicrobial activity of botanical and herbal medicines and screened an herbal product collection against stationary phase B.henselae in vitro using a SYBR Green I/propidium iodide viability assay.We identified five herbal product extracts(at 1.67 mg/mL or 2.5mg/mL),including Cryptolepis sanguinolenta,Juglans nigra,Polygonum cuspidatum,Scutellaria baicalensis,and Scutellaria barbata,that had high activity against stationary phase B.henselae.Among them,C.sanguinolenta,J.nigra,and P.cuspidatum could eradicate all stationary phase B.henselae cells within 7days at 0.83 mg/mL or 1.25 mg/mL in drug exposure time-kill assays,whereas S.baicalensis and S.barbata showed relatively poor activity.These top hits were active against both stationary phase nongrowing B.henselae and log phase growing B.henselae in minimum inhibitory concentration testing.These findings may have implications for improved treatment of persistent Bartonella infections.
文摘The rapid appraisal method was used to identity the botanicals used ethnomedicinally from a total of 300 randomly selected respondents drawn from the existing three geo-political zones of Ekiti State, Nigeria. The results obtained revealed that about 40% of the 71 botanicals identified presently rare. Most of the presently abundant botanicals are species primarily cultivated for other purpose other than medicine. Most of the identified species are valued for their curative effects on malaria and fever, the predominant diseases in the study area. The need for the conservation of the rare species cannot be over emphasised as most rural dwellers in the study area depend mostly on herbs from these species. Strategies towards the attainment of this goal are proposed.
基金Special Foundation for Science and Technology Innovation Strategy of Guangdong Province in 2021(Science and Technology Innovation of College Students Cultivation)(pdjh2021b0940).
文摘The functions of medicinal botanical gardens are introduced firstly,and then the application status and planning principles of medicinal botanical gardens based on the theme of science popularization education are analyzed.Moreover,a typical application case is analyzed and summarized.Constructing a scientific medicinal botanical garden is conducive to increasing people's understanding of medicinal plants,and also meets the requirements of current national policies,thereby generating greater economic and social benefits.
