[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types ...[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types of CWDEs produced by B. theobromae Pat. were studied under both in vitro culture and inocula- tion conditions, along with the pathogenicity and the ability of producing CWDEs of four post-harvest fangal pathogens（B, theobromae Pat. , Colletotrichum gloeos- porioides Penz. , Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif. ） which cause stem-end rot of mangoes. [ Result] B. theobromae Pat. was a- ble to produce polygalacturonase（PG）, pectinmethylgalacturonase（PMG）, polygalacturonic acid trans-eliminase （PGTE）, pectin methyltrans-eliminase （PMTE） and cellulase （ Cx. ） under both in vitro culture and inoculation conditions, of which activities of PG, Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs, the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theo- bromae Pat. was significantly higher than that of any other three pathogens; it is the same with the abilities of producing pectinase. [ Conclusion] This paper pro- vides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits.展开更多
基金Supported by the Special Fund for Agro-scientific Research(3-44-2)the Special Fund for Basic Research of Central Universities in the Public Interest,China(N0.2009hzs1J011,NO.2011hzs1J027,NO.2011hzs1J004)
文摘[ Objective ] This study aimed to confirm the roles of cell wall degrading enzymes （CWDEs） produced by Botryodiplodia theobromae Pat. in the infec- tion of mango fruits. [ Method] Change of activities of five types of CWDEs produced by B. theobromae Pat. were studied under both in vitro culture and inocula- tion conditions, along with the pathogenicity and the ability of producing CWDEs of four post-harvest fangal pathogens（B, theobromae Pat. , Colletotrichum gloeos- porioides Penz. , Phomopsis mangiferae Ahmad and Dothiorella dominicana Pet. et Cif. ） which cause stem-end rot of mangoes. [ Result] B. theobromae Pat. was a- ble to produce polygalacturonase（PG）, pectinmethylgalacturonase（PMG）, polygalacturonic acid trans-eliminase （PGTE）, pectin methyltrans-eliminase （PMTE） and cellulase （ Cx. ） under both in vitro culture and inoculation conditions, of which activities of PG, Cx and PMG were significantly higher in than that in either PGTE or PMTE. Among three primary CWDEs, the peak of activities of PG and Cx appeared earlier and that of PMG occured later. The pathogenicity of B. theo- bromae Pat. was significantly higher than that of any other three pathogens; it is the same with the abilities of producing pectinase. [ Conclusion] This paper pro- vides theoretical bases for further exploring the mechanism of host-pathogen interaction and decreasing the post-harvest loss of mango fruits.
