Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the world.The occurrence of diseases has a great impact on the yield and quality of soybean.In this study,based on the RNA-seq of soybean v...Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the world.The occurrence of diseases has a great impact on the yield and quality of soybean.In this study,based on the RNA-seq of soybean variety M18,a complete CDS(Coding sequence)GmPR1L of the pathogenesis-related protein 1 family was obtained,which has the ability to resist fungal diseases.The overexpression vector and interference expression vector were transferred into tobacco NC89,and the resistance of transgenic tobacco(Nicotiana tabacum L.)to Botrytis cinerea infection was identified.The results show that:Compared with the control,the activities of related defense enzymes SOD(Superoxide dismutase),POD(Peroxidase),PAL(L-phenylalanine ammonia-lyase)and PPO(Polyphenol oxidase)in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees,and increased significantly at different infection time points.The activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control strains.The results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line,and the disease spot rate of OEA2 was significantly lower than that of the control line.The plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control line.It is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to B.cinerea.展开更多
[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determ...[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determined as B.cinerea.The 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in bases.Phylogenetic tree analysis of strain nky007a indicated that nky007a belonged to Botrytis.When healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain B.cinerea.展开更多
基金This work was supported by Major Science and Technology Projects(20210302002NC)Jilin Province Science and Technology Development Plan Project,Grant Number 20190103120JH+2 种基金Jilin Province Science and Technology Development Plan—Outstanding Young Talents Fund Project,Grant Number 20190103120JThe Fourth Batch of Jilin Province Youth Science and Technology Talent Support Project,Grant Number QT202020National Natural Science Foundation of China Projects,Grant Number 31801381.
文摘Soybean(Glycine max(Linn.)Merr.)annual leguminous crop is cultivated all over the world.The occurrence of diseases has a great impact on the yield and quality of soybean.In this study,based on the RNA-seq of soybean variety M18,a complete CDS(Coding sequence)GmPR1L of the pathogenesis-related protein 1 family was obtained,which has the ability to resist fungal diseases.The overexpression vector and interference expression vector were transferred into tobacco NC89,and the resistance of transgenic tobacco(Nicotiana tabacum L.)to Botrytis cinerea infection was identified.The results show that:Compared with the control,the activities of related defense enzymes SOD(Superoxide dismutase),POD(Peroxidase),PAL(L-phenylalanine ammonia-lyase)and PPO(Polyphenol oxidase)in the over-expressed transgenic tobacco OEA1 and OEA2 increased to different degrees,and increased significantly at different infection time points.The activities of defense enzymes in the interfering strains IEA1 and IEA2 were significantly lower than those in the control strains.The results of resistance level identification showed that the disease spot rate of OEA1 was significantly lower than that of the control line,and the disease spot rate of OEA2 was significantly lower than that of the control line.The plaque rate of the interfering expression line IEA1-IEA2 was significantly higher than that of the control line.It is preliminarily believed that the process related protein GmPR1L can improve the resistance of tobacco to B.cinerea.
基金Science and Technology Guidance Program of Xinjiang Academy of Agricultural Reclamation Sciences(2020YJ019).
文摘[Objective]The paper was to isolate and identify Botrytis cinerea from processing tomato.[Method]The strain nky007a was isolated and purified from fruits infected by tomato grey mold.After identification,it was determined as B.cinerea.The 18S rDNA and internal transcriptional spacer(ITS)sequences of strain nky007a were amplified for molecular identification and classification.[Result]The 18S rDNA sequence of the strain shared the homology of 99.95%with Botryotinia fuckeliana(EF 110887.1);the ITS rDNA sequence had the homology of 100%with Botryotinia fuckeliana(AB444949.1),and there was no difference in bases.Phylogenetic tree analysis of strain nky007a indicated that nky007a belonged to Botrytis.When healthy tomatoes were infected by the strain,the incidence rate of tomato grey mold was up to 95%,indicating the pathogenicity of the strain.[Conclusion]Morphological identification,molecular identification and pathogenicity analysis results show that the strain nky007a is a highly pathogenetic strain B.cinerea.
