Objective:To study the effect of temperature on the morphological characteristics of Botrytiscinerea(B.cinerea)and its correlated with the genetic variability.B.cinerea is a plant-pathogenic fungus that produces the d...Objective:To study the effect of temperature on the morphological characteristics of Botrytiscinerea(B.cinerea)and its correlated with the genetic variability.B.cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries.Methods:Six strains from different host collected have been isolated and characterized by several methods as mycelial growth,fungicide resistance,pathogenicity and the effects of the temperature.Also was analyzed by PCR and distinguished by the presence or absence of transposable elements.Results:Results showed that clear morphological differences exist between strains at the temperature of 4,12 and 28℃.All strains analyzed molecularly were classified as Group II(transposa-type).Demonstrating a negative correlation between mycelial growth and other characteristics as the fungicide resistance and pathogenicity.Lastly,it is difficult to establish relationships phenotypic and genotypic between strains of B.cinerea.Conclusions:The results indicated that the mycelial growth,resistance at fungicide and pathogenicity are independent of the characteristics molecular,however,are dependent of a factor such as temperature.展开更多
Objective:To detect Botrytis cinerea(B.cinerea)latent infections on apples before storage,which is essential for effective control strategies in the fruit postharvest industry.Methods:In the present study,a polymerase...Objective:To detect Botrytis cinerea(B.cinerea)latent infections on apples before storage,which is essential for effective control strategies in the fruit postharvest industry.Methods:In the present study,a polymerase chain reaction detection method,based on primers designed on B.cinerea transposable elements(boty and flipper)and intergenic spacer region as internal control,were utilized to reveal the presence of symptomless infections on apple fruits.This molecular method proved to be highly specific and sensitive in detecting latent infections.It revealed the presence of the pathogen in 83%of the samples from infected apples with 104 conidia/mL,whereas those infected with 106 conidia/mL detected 94%as compared to the traditional method that revealed the pathogen in 40%and 66%of the samples inoculated with 10^(4)and 10^(6)conidia/mL respectively.Furthermore,the method characterized B.cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results:The results obtained from DNA quantification method were compared with enzyme-linked immunosorbent assay and these studies showed good correlation.Therefore our method has important advantages compared with others detection methods for B.cinerea,because the proposed methodology allowed distinguishes between its two subpopulations(vacuma and transposa)and this would allow establish possible appropriate control strategies.Conclusions:Finally,the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.展开更多
基金Supported by National University of San Luis(PROICO 22/Q241)the National Agency of Scientific and Technologic Promotion(PICT-2011-0459)the National Scientific and Technical Research Council(CONICET)(PIP 112-201101-00114).
文摘Objective:To study the effect of temperature on the morphological characteristics of Botrytiscinerea(B.cinerea)and its correlated with the genetic variability.B.cinerea is a plant-pathogenic fungus that produces the disease known as grey mould in a wide variety of agriculturally important hosts in many countries.Methods:Six strains from different host collected have been isolated and characterized by several methods as mycelial growth,fungicide resistance,pathogenicity and the effects of the temperature.Also was analyzed by PCR and distinguished by the presence or absence of transposable elements.Results:Results showed that clear morphological differences exist between strains at the temperature of 4,12 and 28℃.All strains analyzed molecularly were classified as Group II(transposa-type).Demonstrating a negative correlation between mycelial growth and other characteristics as the fungicide resistance and pathogenicity.Lastly,it is difficult to establish relationships phenotypic and genotypic between strains of B.cinerea.Conclusions:The results indicated that the mycelial growth,resistance at fungicide and pathogenicity are independent of the characteristics molecular,however,are dependent of a factor such as temperature.
基金Supported by National University of San Luis(PROICO 22/Q241)the National Agency of Scientific and Technologic Promotion(PICT–2011-0459)the National Scientific and Technical Research Council(CONICET)(PIP 112-201101-00114).
文摘Objective:To detect Botrytis cinerea(B.cinerea)latent infections on apples before storage,which is essential for effective control strategies in the fruit postharvest industry.Methods:In the present study,a polymerase chain reaction detection method,based on primers designed on B.cinerea transposable elements(boty and flipper)and intergenic spacer region as internal control,were utilized to reveal the presence of symptomless infections on apple fruits.This molecular method proved to be highly specific and sensitive in detecting latent infections.It revealed the presence of the pathogen in 83%of the samples from infected apples with 104 conidia/mL,whereas those infected with 106 conidia/mL detected 94%as compared to the traditional method that revealed the pathogen in 40%and 66%of the samples inoculated with 10^(4)and 10^(6)conidia/mL respectively.Furthermore,the method characterized B.cinerea as subpopulation transposa-type by the presence of the transposable elements boty and flipper Results:The results obtained from DNA quantification method were compared with enzyme-linked immunosorbent assay and these studies showed good correlation.Therefore our method has important advantages compared with others detection methods for B.cinerea,because the proposed methodology allowed distinguishes between its two subpopulations(vacuma and transposa)and this would allow establish possible appropriate control strategies.Conclusions:Finally,the method can be an interesting alternative for its possible application in the phytosanitary programs of the fruit industry worldwide.
