Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. A...Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. As a conjugation specific cyclin gene, CYC2 knockout mutants failed to form an elongated crescent structure and aborted meiosis progress in T. thermophila. γ-H2 A.X staining revealed fewer micronuclear DNA double-strand breaks(DSBs) in cyc2Δ cells than in wild-type cells. Furthermore, cyc2Δ cells still failed to form a crescent structure even though DSBs were induced by exogenous agents,indicating that a lack of DSBs was not completely responsible for failure to enter the crescent stage. Tubulin staining showed that impaired perinuclear microtubule structure may contribute to the blockage in micronuclear elongation. At the same time,expression of microtubule-associated kinesin genes, KIN11 and KIN141, was significantly downregulated in cyc2Δ cells.Moreover, micronuclear specific accumulation of heterochromatin marker trimethylated H3 K23 abnormally increased in the cyc2Δ mutants. Together, these results show that cyclin Cyc2 p is required for micronuclear bouquet formation via controlling microtubule-directed nuclear elongation in Tetrahymena.展开更多
Nous sommes entrés dans le magasin,j'ai mis tout mon argent sur le comptoir et j'ai dit à la dame que je voulais un très gros bouquet de fleurs pour ma maman,mais pas des roses parce qu'il y...Nous sommes entrés dans le magasin,j'ai mis tout mon argent sur le comptoir et j'ai dit à la dame que je voulais un très gros bouquet de fleurs pour ma maman,mais pas des roses parce qu'il y en a beaucoup dans notre jardin et ce n'est pas la peine d'aller en acheter ailleurs.展开更多
基金supported by the National Natural Science Foundation of China(31471999,31572253)Shanxi Scholarship Council of China(200902)+2 种基金Aoshan Science and Technology Innovation Program of the Qingdao National Laboratory for Marine Science and Technologythe China Postdoctoral Science Foundation(2014M551961)the Natural Science Foundation of Shanxi Province(2015011078)
文摘Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. As a conjugation specific cyclin gene, CYC2 knockout mutants failed to form an elongated crescent structure and aborted meiosis progress in T. thermophila. γ-H2 A.X staining revealed fewer micronuclear DNA double-strand breaks(DSBs) in cyc2Δ cells than in wild-type cells. Furthermore, cyc2Δ cells still failed to form a crescent structure even though DSBs were induced by exogenous agents,indicating that a lack of DSBs was not completely responsible for failure to enter the crescent stage. Tubulin staining showed that impaired perinuclear microtubule structure may contribute to the blockage in micronuclear elongation. At the same time,expression of microtubule-associated kinesin genes, KIN11 and KIN141, was significantly downregulated in cyc2Δ cells.Moreover, micronuclear specific accumulation of heterochromatin marker trimethylated H3 K23 abnormally increased in the cyc2Δ mutants. Together, these results show that cyclin Cyc2 p is required for micronuclear bouquet formation via controlling microtubule-directed nuclear elongation in Tetrahymena.
文摘Nous sommes entrés dans le magasin,j'ai mis tout mon argent sur le comptoir et j'ai dit à la dame que je voulais un très gros bouquet de fleurs pour ma maman,mais pas des roses parce qu'il y en a beaucoup dans notre jardin et ce n'est pas la peine d'aller en acheter ailleurs.
