Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and e...Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and estrone sulfate),but sometimes they provide false-positive results.Embryo specific pregnancy markers,which delineate the presence and viability of the embryo,are considered as perfect for pregnancy determination.Pregnancy-associated glycoproteins are distinguished as the best indicator for the determination of early pregnancy,fetal number,and birth weight of kids.Pregnancy-associated glycoproteins are structurally correlated to aspartic proteinase and are communicated in the external epithelial cell layer of the placenta.They have been found to share about half amino acid sequence identity with pepsinogen,pepsin,cathepsin D and E.Dislike different individuals from aspartic proteinase family,numerous pregnancy-associated glycoproteins appear to be latent compound as a result of amino acid substitutions in and around the catalytic site.This review is to discuss the scope and prospects of pregnancy-associated glycoproteins as a pregnancy marker in farm animals,more specifically in goats.展开更多
By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced.The nucleotide sequence of this gD gene was (1 251 bp,)encoding 417 amino acids.Comparied with the...By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced.The nucleotide sequence of this gD gene was (1 251 bp,)encoding 417 amino acids.Comparied with the published P8-2 strain,the homology of the necleotide sequence is 99.92%,and that of the deduced amino acid sequence is 100%.The results indicated that gD of BHV-1 was highly conservative.展开更多
文摘Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and estrone sulfate),but sometimes they provide false-positive results.Embryo specific pregnancy markers,which delineate the presence and viability of the embryo,are considered as perfect for pregnancy determination.Pregnancy-associated glycoproteins are distinguished as the best indicator for the determination of early pregnancy,fetal number,and birth weight of kids.Pregnancy-associated glycoproteins are structurally correlated to aspartic proteinase and are communicated in the external epithelial cell layer of the placenta.They have been found to share about half amino acid sequence identity with pepsinogen,pepsin,cathepsin D and E.Dislike different individuals from aspartic proteinase family,numerous pregnancy-associated glycoproteins appear to be latent compound as a result of amino acid substitutions in and around the catalytic site.This review is to discuss the scope and prospects of pregnancy-associated glycoproteins as a pregnancy marker in farm animals,more specifically in goats.
文摘By means of PCR,the gene encoding gD of bovine herpesvirus-1 (BHV-1) strain Luojing was amplified,cloned and sequenced.The nucleotide sequence of this gD gene was (1 251 bp,)encoding 417 amino acids.Comparied with the published P8-2 strain,the homology of the necleotide sequence is 99.92%,and that of the deduced amino acid sequence is 100%.The results indicated that gD of BHV-1 was highly conservative.
