Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four importan...Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.展开更多
Ultra fine grinding of the plant tailings of a refractory silver ore was studied using a laboratory type vertical stirred media mill. Preliminary tests confirmed that ultra fine grinding substantially improves the ext...Ultra fine grinding of the plant tailings of a refractory silver ore was studied using a laboratory type vertical stirred media mill. Preliminary tests confirmed that ultra fine grinding substantially improves the extraction of silver from the tailings in cyanide leaching （i.e. 36% Ag extraction rate from the as-received tailings with d80 of 100 μm, c.f. 84% extraction rate after ultra fine grinding of the tailings with ds0 of 1.2 pro）. In the ultra fine grinding tests, the effects of ball diameter （2-4.5 mm）, stirring speed （200-800 r/m/n） and ball charge ratio （50%-80%） on the fineness of grind （ds0, ~tm） were investigated through a Box-Behnken design. Increasing stirrer speed and ball charge ratio decreased fineness of grind while larger balls resulted in the coarser products. The tests demonstrated that a fineness of grind less than 5 μm can be achieved under suitable conditions. Analysis of stress intensity indicated an optimum range of stress intensity of （0.8-2）× 10^- 3 μm for all power inputs.展开更多
[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA con...[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.展开更多
Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin e...Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.展开更多
基金Supported by the National Natural Science Foundation of China （21036005）.
文摘Disulfide bond formation protein A （DsbA） is one of the important helper proteins for folding in protein synthesis in vivo. In this study, purification of recombinant DsbA was investigated by examining four important factors with Box-Behnken design method, a statistic-based design of experiments. The optimal operation conditions were obtained by adopting the effectiveness coefficient method on the multi-objective problem, which takes the protein recovery, purification efficiency and throughput of ion-exchange chromatography into account. After the optimization, protein recovery of 96.8% and purity higher than 95% DsbA was achieved, and the productivity was （377.9±1.7） mg soluble DsbA per liter broth. The purified protein was identified by peptide mass fingerprinting matching the record of gil2624856, a mutant of DsbA. The DsbA was preliminarily applied to the refolding of denatured lysozyme in vitro.
文摘Ultra fine grinding of the plant tailings of a refractory silver ore was studied using a laboratory type vertical stirred media mill. Preliminary tests confirmed that ultra fine grinding substantially improves the extraction of silver from the tailings in cyanide leaching （i.e. 36% Ag extraction rate from the as-received tailings with d80 of 100 μm, c.f. 84% extraction rate after ultra fine grinding of the tailings with ds0 of 1.2 pro）. In the ultra fine grinding tests, the effects of ball diameter （2-4.5 mm）, stirring speed （200-800 r/m/n） and ball charge ratio （50%-80%） on the fineness of grind （ds0, ~tm） were investigated through a Box-Behnken design. Increasing stirrer speed and ball charge ratio decreased fineness of grind while larger balls resulted in the coarser products. The tests demonstrated that a fineness of grind less than 5 μm can be achieved under suitable conditions. Analysis of stress intensity indicated an optimum range of stress intensity of （0.8-2）× 10^- 3 μm for all power inputs.
基金Supported by Scientific and Technological Planning Project of Pu'er City of Yunnan Province(2012kj007)
文摘[Objectives] To increase the survival rate of tissue culture seedlings of Dendrobium officinale,and optimize the conditions of rooting medium by the response surface methodology( RSM). [Methods]The effects of 6-BA concentration,NAA concentration,potato amount and the amount of mashed banana on the growth of seedlings were determined by single factor experiment and were analyzed by Box-Behnken design and response surface methodology. [Results]The optimal culture conditions: rooting medium 1/2 MS + 6-BA 0. 24 mg/L + mashed banana 87. 63 g/L + potato 89. 30 g/L + NAA 0. 52 mg/L + sucrose 20. 0 g/L + activated carbon 4. 0 g/L + agar 7. 0 g/L,p H 5. 8,and light intensity 2 000 Lx. [Conclusions]The model established by response surface methodology has a good predictability and could be used to optimize the conditions of tissue culture and rooting medium of D. officinale.
基金supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0501209)Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2013-OCRI)
文摘Melanin involves in various biological functions, widely used in cosmetic, pharmacology medicine and other fields. However, melanin application is limited due to low productivity and high cost. In this work, melanin extraction method from rapeseed meal(RSM) was explored. Effects of hydrochloric acid(HCl) concentration, ethanol concentration, extraction temperature and time, and solid-liquid ratio were evaluated systematically. According to results of single factor experiment and Box-Behnken experiment, the optimum extraction conditions of melanin from RSM were as follows: HCl concentration, 0.5 mol/L; ethanol concentration, 70%; extraction temperature, 40 o C; extraction time, 1 h; solid liquid ratio, 1/4 g/mL. Under these conditions, extraction yields of crude melanin(CM) and purified melanin(PM) were 9.00% and 1.44%, respectively. Compared with synthetic melanin(SM) from Sigma, the relative purities of CM and PM were 7.82% and 29.57%, respectively. Moreover, feedstuff value of RSM residue was also improved after melanin isolation by significantly reducing glucosinolate content.
