Effect of cyproheptadine on changes of 5-HT and 5-HIAA levels in brain cortex and serum following rat brain injury

Changes of 5-hydroxytryptamine(5-HT)and 5-hydroxyindoleacetic acid(5-HIAA)levels in brain cortex and serum were studied using spectrofluorometer follow-ing brain injury in rats.Cyproheptadine,a receptor antagonist of 5-HT,was employedand its effect on alterations of 5-HT and 5-HIAA contents in brain cortex and serum af-ter brain injury was investigated.The results showed that after brain injury levels of5-HT and 5-HIAA in the brain cortex increased markedly,The increase of 5-HT in thebrain cortex reached its peak at 48h after brain injury,and its value was 4.29±0.44μg/g,which was 4.8 times of control value.The increase of 5-HIAA in the brain cortexreached its peak at 24h after brain injury,and its value was 5.48±0.41μg/g,which was5.8times of control value.The change of 5-HT in the serum was not significant,but thelevel of 5-HIAA in the serum was increased significantly.Cyproheptadine could reducelevels of 5-HT and 5-HIAA in this experiment.The value of 5-HT in the brain cortexdecreased to 1.32±0.09μg/g,and the value of 5-HIAA decreased to 1.36±0.10μg/g.Thelevel of 5-HIAA in the serum was also reduced significantly.It was 0.27±0.03μg/ml.

赛庚啶对大鼠脑损伤脑皮质和脑微血管中5-HT含量的影响

采用高效液相色谱-电化学检测器检测大鼠（180只）脑损伤后脑皮质及脑微血管中5－HT含量变化，并选用5-HT受体阻断剂赛庚啶治疗，观察其对脑损伤后脑皮质及脑微血管5－HT水平变化的影响。结果表明：脑损伤后5－HT水平显著升高，神经细胞和脑微血管内皮细胞病理变化与5－HT代谢改变有密切关系。赛庚啶可显著降低脑皮质及脑微血管中5－HT含量，并减轻脑继发性损害。