Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar...Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar rats were used in this study, and the bilateral E-tubes of each rat were examined. Ten E-tubes were used for anatomical studies, another ten for histological analysis, and the other ten for Eustachian tubography. Five rats were euthanized and decapitated, and ten E-tubes were dissected to describe the anatomy of the E-tube. Ten E-tube specimens obtained from five other rats were sectioned to investigate Etube histology. Eustachian tubography was performed on the bilateral E-tubes of the other five rats using the trans-tympanic approach.Results: The rat E-tubes consisted of bony and membranous parts. Cartilage and bone tissue covered only the bony part. The E-tubes’ mean diameter and overall length were 2.97 mm and 4.96 mm, respectively. The tympanic orifices’ mean diameter was 1.21 mm. The epithelium of E-tubes was mainly composed of pseudostratified ciliated and goblet cells. Eustachian tubography was successfully performed on both sides of the E-tube for each rat.The technical success rate was 100%, the average running time was 4.9 min, and no procedure-related complications occurred. On tubography images, the E-tube, tympanic cavity, and nasopharynx could be identified because of the visualization of bony landmarks.Conclusion: In this study, we described the anatomical and histological features of rat E-tubes. With the aid of these findings, E-tube angiography was successfully performed using a transtympanic approach. These results will facilitate further investigation of E-tube dysfunction.展开更多
Emil Theodor Kocher and Theodor Billroth pioneered the surgical management of thyroid disease. Their surgical techniques, knowledge of thyroid anatomy, embryology, histology, physiology, and antisepsis practices trans...Emil Theodor Kocher and Theodor Billroth pioneered the surgical management of thyroid disease. Their surgical techniques, knowledge of thyroid anatomy, embryology, histology, physiology, and antisepsis practices transitioned a life-threatening operation to one with acceptable morbidity. The modern head and neck surgeon should have a meticulous surgical technique, combined with a thorough understanding of thyroid embryology and anatomy that is central to the understanding and treatment of the different disease processes of the thyroid gland and the consequences of thyroid gland surgery. In this manuscript we will be examining thyroid gland embryology, anatomy, histology, and physiology that is essential to the practicing thyroid surgeon.展开更多
Olive oil is an important source of mono-unsaturated fat and a prime component of the Mediterranean diet. The beneficial health effects of olive oil are due to both its high content of mono-unsaturated fatty acids and...Olive oil is an important source of mono-unsaturated fat and a prime component of the Mediterranean diet. The beneficial health effects of olive oil are due to both its high content of mono-unsaturated fatty acids and its high content of anti-oxidative substances. The objective of this study was to investigate the basis for the epidemiological information relating to the health benefits associated with the consumption of ex-tra-virgin olive oil (EVOO). The effect of olive oil on norepinephrine (NE), dopamine (DA), serotonin (5-HT) and gamm-aminobutyric acid (GABA) con-tents in different brain regions and histological structure of liver and kindey of male albino rats was studied. The chronic administration of olive oil (7.5 mg/kg body wt.) caused a significant increase in norepinephrine (NE), dopamine (DA) , serotonin (5-HT) and gamm-aminobutyric acid (GABA) con-tent in different brain regions (Cerebellum, striatum, cerebral cortex, hypothalamus, brain steam and hip-pocampus) of male albino rats. The increase in NE, DA, 5-HT, and GABA content in the different CNS areas of male albino rat may be due to the inhibition of Ca2+/calmodulin binding which plays an important role in the release of these neurotransmitters. The results, also, revealed that urea and creatinne con-centrations in rats with oral administration with olive oil were decreased. Meanwhile, the activities of the enzymes AsT, AlT and ALP were elevated. The pre-sent results indicated that there is no change in tis-sues of kidney after treated with virgin olive oil. Olive oil may potentially be safe for use as a sedative drug. improvement also led to the reductions in risk of Alzheimer’s and Parkinson’s diseases.展开更多
The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and...The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.展开更多
Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are...Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are limited by the opacity of brain tissue.In this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve injury.Specifically,we performed light-sheet imaging of whole brain tissue after a clearing process.We found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared brains.Unexpectedly,PRV724-traced neurons were more widely distributed compared with data from previous studies.We found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve nucleus.Our findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve injury.Thus,our approach may be useful for future estimations of optic nerve injury and regeneration.展开更多
In the past two decades,pain perception in the human brain has been studied with EEG/MEG brain topography and PET/fMRI neuroimaging techniques.A host of cortical and subcortical loci can be activated by various nocice...In the past two decades,pain perception in the human brain has been studied with EEG/MEG brain topography and PET/fMRI neuroimaging techniques.A host of cortical and subcortical loci can be activated by various nociceptive conditions.The activation in pain perception can be induced by physical(electrical,thermal,mechanical),chemical(capsacin,ascoric acid),psychological(anxiety,stress,nocebo) means,and pathological(e.g.migraine,neuropathic) diseases.This article deals mainly on the activation,but not modulation,of human pain in the brain.The brain areas identified are named pain representation,matrix,neuraxis,or signature.The sites are not uniformly isolated across various studies,but largely include a set of cores sites:thalamus and primary somatic area(SI),second somatic area(SII),insular cortex(IC),prefrontal cortex(PFC),cingulate,and parietal cortices.Other areas less reported and considered important in pain perception include brainstem,hippocampus,amygdala and supplementary motor area(SMA).The issues of pain perception basically encompass both the site and the mode of brain function.Although the site issue is delineared to a large degree,the mode issue has been much less explored.From the temporal dynamics,IC can be considered as the initial stage in genesis of pain perception as conscious suffering,the unique aversion in the human brain.展开更多
Unregulated overproduction of parathyroid hormone (PTH) from an abnormal parathyroid gland is the origin of primary hyperparathyroidism (PHPT). Patients who have an elevated serum calcium concentration have a problem ...Unregulated overproduction of parathyroid hormone (PTH) from an abnormal parathyroid gland is the origin of primary hyperparathyroidism (PHPT). Patients who have an elevated serum calcium concentration have a problem in one or more of their parathyroid glands. To understand this disease state adequately and provide appropriate treatment, a thorough understanding of parathyroid embryology, anatomy, physiology, and pathophysiology is essential. In this manuscript, we review our current understanding of parathyroid gland embryology, anatomy, histology, physiology, and pathophysiology.展开更多
盆腔器官脱垂(pelvic organ prolapse,POP)是一种由多因素介导的骨盆支持结构减弱引起的疾病,其主要危险因素是多胎妊娠和阴道分娩,然而POP的病理生理学过程目前尚不清楚。手术是治疗中重度POP的主要方法之一,与子宫骶韧带(uterosacral ...盆腔器官脱垂(pelvic organ prolapse,POP)是一种由多因素介导的骨盆支持结构减弱引起的疾病,其主要危险因素是多胎妊娠和阴道分娩,然而POP的病理生理学过程目前尚不清楚。手术是治疗中重度POP的主要方法之一,与子宫骶韧带(uterosacral ligament,USL)相关的术式对恢复顶端支持具有重要作用,且手术成功率高。然而,术后复发和并发症的发生风险依然存在,这主要是因为术者对USL的解剖组织学和生物力学知识缺乏足够了解。准确认识USL的解剖位置和组织构成不仅有助于寻找USL最适合悬吊的位点,还可以避免术中损伤邻近的器官组织,如输尿管、直肠和神经血管等。此外,对USL各部分生物力学性能的全面认识还可以为POP发病机制的研究、预防策略和个性化手术的制定提出新的解决办法。展开更多
Dental anatomy and histology is one of the fundamental subjects and a key to comprehend the act of dentistry.Dental features such as tooth morphology and variations in shape and size play a vital role in the area of f...Dental anatomy and histology is one of the fundamental subjects and a key to comprehend the act of dentistry.Dental features such as tooth morphology and variations in shape and size play a vital role in the area of forensic odontology.Forensic odontology is a challenging field that involves the application of such knowledge of dental sciences in the identification of deceased individuals.The aim and objective was to assess and evaluate the knowledge,attitude,and practice regarding dental anatomy and histology in forensic investigation among dental practitioners.A cross-sectional survey was carried out among 217 dental practitioners from Maharashtra who in their day-to-day life may experience instances of forensic interest.The information was collected through telephonic interview by means of a pretested,close-ended questionnaire.In our stud%the general awareness about forensic odontology was found to be more among MDS dental practitioners than BDS dental practitioners.Overall,there is a lack of adequate legal knowledge and forensic practices among the dentists of Mumbai and Nagpur.However,the practitioners did have a positive attitude toward the field and were keen to learn more about it.The study is the first of its kind to be conducted in Maharashtra state of India regarding dental anatomy and histology with respect to forensic odontology.The dental practitioners were also aware about the dental anatomy and dental histology with respect to forensic investigation.展开更多
基金funding from the Korea Health Technology R&D Project, Ministry for Health, Welfare and Family Affairs, Republic of Korea (HI17C0881)。
文摘Purpose: This study investigated the anatomical and histological characteristics of the rat Eustachian tube(E-tube)and the feasibility of Eustachian tubography in a rat model.Materials and methods: Fifteen male Wistar rats were used in this study, and the bilateral E-tubes of each rat were examined. Ten E-tubes were used for anatomical studies, another ten for histological analysis, and the other ten for Eustachian tubography. Five rats were euthanized and decapitated, and ten E-tubes were dissected to describe the anatomy of the E-tube. Ten E-tube specimens obtained from five other rats were sectioned to investigate Etube histology. Eustachian tubography was performed on the bilateral E-tubes of the other five rats using the trans-tympanic approach.Results: The rat E-tubes consisted of bony and membranous parts. Cartilage and bone tissue covered only the bony part. The E-tubes’ mean diameter and overall length were 2.97 mm and 4.96 mm, respectively. The tympanic orifices’ mean diameter was 1.21 mm. The epithelium of E-tubes was mainly composed of pseudostratified ciliated and goblet cells. Eustachian tubography was successfully performed on both sides of the E-tube for each rat.The technical success rate was 100%, the average running time was 4.9 min, and no procedure-related complications occurred. On tubography images, the E-tube, tympanic cavity, and nasopharynx could be identified because of the visualization of bony landmarks.Conclusion: In this study, we described the anatomical and histological features of rat E-tubes. With the aid of these findings, E-tube angiography was successfully performed using a transtympanic approach. These results will facilitate further investigation of E-tube dysfunction.
文摘Emil Theodor Kocher and Theodor Billroth pioneered the surgical management of thyroid disease. Their surgical techniques, knowledge of thyroid anatomy, embryology, histology, physiology, and antisepsis practices transitioned a life-threatening operation to one with acceptable morbidity. The modern head and neck surgeon should have a meticulous surgical technique, combined with a thorough understanding of thyroid embryology and anatomy that is central to the understanding and treatment of the different disease processes of the thyroid gland and the consequences of thyroid gland surgery. In this manuscript we will be examining thyroid gland embryology, anatomy, histology, and physiology that is essential to the practicing thyroid surgeon.
文摘Olive oil is an important source of mono-unsaturated fat and a prime component of the Mediterranean diet. The beneficial health effects of olive oil are due to both its high content of mono-unsaturated fatty acids and its high content of anti-oxidative substances. The objective of this study was to investigate the basis for the epidemiological information relating to the health benefits associated with the consumption of ex-tra-virgin olive oil (EVOO). The effect of olive oil on norepinephrine (NE), dopamine (DA), serotonin (5-HT) and gamm-aminobutyric acid (GABA) con-tents in different brain regions and histological structure of liver and kindey of male albino rats was studied. The chronic administration of olive oil (7.5 mg/kg body wt.) caused a significant increase in norepinephrine (NE), dopamine (DA) , serotonin (5-HT) and gamm-aminobutyric acid (GABA) con-tent in different brain regions (Cerebellum, striatum, cerebral cortex, hypothalamus, brain steam and hip-pocampus) of male albino rats. The increase in NE, DA, 5-HT, and GABA content in the different CNS areas of male albino rat may be due to the inhibition of Ca2+/calmodulin binding which plays an important role in the release of these neurotransmitters. The results, also, revealed that urea and creatinne con-centrations in rats with oral administration with olive oil were decreased. Meanwhile, the activities of the enzymes AsT, AlT and ALP were elevated. The pre-sent results indicated that there is no change in tis-sues of kidney after treated with virgin olive oil. Olive oil may potentially be safe for use as a sedative drug. improvement also led to the reductions in risk of Alzheimer’s and Parkinson’s diseases.
基金The National Natural Science Foundation of China under contract Nos 31802319,31972784,31802269the Natural Science Foundation of Shandong Province under contract No.ZR2018BC053+1 种基金the Agricultural Application Technology Innovation Project of Shandong Province under contract No.SD2019YY006the Advanced Talents Foundation of Qingdao Agricultural University under contract Nos 6631119055,6631119032.
文摘The brain plays a critical role in controlling reproduction through the hypothalamus-pituitary-gonadal(HPG)axis in vertebrates.Turbot(Scophthalmus maximus)has become an economically important marine fish in Europe and North China.Previous research investigating turbot reproduction has focused on the role of the HPG axis in regulating egg and sperm production.However,the morphology and histology of the organs in the HPG axis have not been studied.In this study,we investigated the morphology and histology of brains in female and male turbot at different stages of gonadal development.The results showed that the brains of both female and male turbot were composed of seven parts that are typical of advanced teleosts:the telencephalon,diencephalon,cerebellum,hypothalamus,pituitary gland,myelencephalon,and olfactory bulbs.The telencephalon was well-developed and contained five distinct lobes,with the contiguous diencephalon at the caudal portion.The torus longitudinales and rostral torus semicircularis of the mesencephalon flattened along the dorsal surface,and the rostral corpus cerebellum was located in the dorsal portion.The actual total brain volume in mature males was significantly greater(p<0.05)than that of females with gonadal development.Notably,the pituitary volume in male turbot significantly increased(p<0.05)from immature to mature stage,but this difference did not occur in females.The data together illustrate a distinct sex difference in the turbot brain during gonadal development,providing insight into their HPG axes.
基金supported by the National Natural Science Foundation of China,No.81870655(to MBY)。
文摘Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are limited by the opacity of brain tissue.In this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve injury.Specifically,we performed light-sheet imaging of whole brain tissue after a clearing process.We found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared brains.Unexpectedly,PRV724-traced neurons were more widely distributed compared with data from previous studies.We found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve nucleus.Our findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve injury.Thus,our approach may be useful for future estimations of optic nerve injury and regeneration.
基金grants from the National Natural Science Foundation of China(No.30770691)Beijing Municipal Government for Advancement of Sciences and Capital Medical University for Innovation Awards
文摘In the past two decades,pain perception in the human brain has been studied with EEG/MEG brain topography and PET/fMRI neuroimaging techniques.A host of cortical and subcortical loci can be activated by various nociceptive conditions.The activation in pain perception can be induced by physical(electrical,thermal,mechanical),chemical(capsacin,ascoric acid),psychological(anxiety,stress,nocebo) means,and pathological(e.g.migraine,neuropathic) diseases.This article deals mainly on the activation,but not modulation,of human pain in the brain.The brain areas identified are named pain representation,matrix,neuraxis,or signature.The sites are not uniformly isolated across various studies,but largely include a set of cores sites:thalamus and primary somatic area(SI),second somatic area(SII),insular cortex(IC),prefrontal cortex(PFC),cingulate,and parietal cortices.Other areas less reported and considered important in pain perception include brainstem,hippocampus,amygdala and supplementary motor area(SMA).The issues of pain perception basically encompass both the site and the mode of brain function.Although the site issue is delineared to a large degree,the mode issue has been much less explored.From the temporal dynamics,IC can be considered as the initial stage in genesis of pain perception as conscious suffering,the unique aversion in the human brain.
文摘Unregulated overproduction of parathyroid hormone (PTH) from an abnormal parathyroid gland is the origin of primary hyperparathyroidism (PHPT). Patients who have an elevated serum calcium concentration have a problem in one or more of their parathyroid glands. To understand this disease state adequately and provide appropriate treatment, a thorough understanding of parathyroid embryology, anatomy, physiology, and pathophysiology is essential. In this manuscript, we review our current understanding of parathyroid gland embryology, anatomy, histology, physiology, and pathophysiology.
文摘盆腔器官脱垂(pelvic organ prolapse,POP)是一种由多因素介导的骨盆支持结构减弱引起的疾病,其主要危险因素是多胎妊娠和阴道分娩,然而POP的病理生理学过程目前尚不清楚。手术是治疗中重度POP的主要方法之一,与子宫骶韧带(uterosacral ligament,USL)相关的术式对恢复顶端支持具有重要作用,且手术成功率高。然而,术后复发和并发症的发生风险依然存在,这主要是因为术者对USL的解剖组织学和生物力学知识缺乏足够了解。准确认识USL的解剖位置和组织构成不仅有助于寻找USL最适合悬吊的位点,还可以避免术中损伤邻近的器官组织,如输尿管、直肠和神经血管等。此外,对USL各部分生物力学性能的全面认识还可以为POP发病机制的研究、预防策略和个性化手术的制定提出新的解决办法。
文摘Dental anatomy and histology is one of the fundamental subjects and a key to comprehend the act of dentistry.Dental features such as tooth morphology and variations in shape and size play a vital role in the area of forensic odontology.Forensic odontology is a challenging field that involves the application of such knowledge of dental sciences in the identification of deceased individuals.The aim and objective was to assess and evaluate the knowledge,attitude,and practice regarding dental anatomy and histology in forensic investigation among dental practitioners.A cross-sectional survey was carried out among 217 dental practitioners from Maharashtra who in their day-to-day life may experience instances of forensic interest.The information was collected through telephonic interview by means of a pretested,close-ended questionnaire.In our stud%the general awareness about forensic odontology was found to be more among MDS dental practitioners than BDS dental practitioners.Overall,there is a lack of adequate legal knowledge and forensic practices among the dentists of Mumbai and Nagpur.However,the practitioners did have a positive attitude toward the field and were keen to learn more about it.The study is the first of its kind to be conducted in Maharashtra state of India regarding dental anatomy and histology with respect to forensic odontology.The dental practitioners were also aware about the dental anatomy and dental histology with respect to forensic investigation.