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Chemical Constituents from Portulaca oleracea L. 被引量:11
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作者 孙健 张宏桂 +3 位作者 张静敏 张天斌 郭滨 张寒琦 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第4期291-292,共2页
Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and ant... Portulaca oleracea L. is distributed widely in China and some othercountries, which is included in the pharmacopoeias of China. In tradition, it is used in thetreatment of atheroma, and also used as antifungal and antiviral agents now. It also decreases bloodsugar, enhances immunity and adjusts blood lipids. The anticancer and antifungal effects ofPortulaca oleracea L. were reported in several patents. But no active component has been found. Inthis study, the chemical compositions from Portulaca oleracea L. were investigated to provideevidence for the activity of Portulaca oleracea L. . 展开更多
关键词 portulaca oleracea l. X-ray diffraction friedelan-4-α-methyl-3β-OH
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根癌农杆菌介导白细胞介素-4基因转化甘蓝(Brassica oleracea L.)研究 被引量:2
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作者 李然红 于丽杰 +1 位作者 陶雷 王雷 《分子植物育种》 CAS CSCD 2007年第1期47-53,共7页
利用根癌农杆菌介导法,以白细胞介素-4(interleukin-4,IL-4)基因转化中甘11号甘蓝。本研究对可能影响il-4转化率的外植体类型、外植体的预培养时间、农杆菌的侵染时间以及外植体与农杆菌的共培养时间进行了优化。试验结果表明:il-... 利用根癌农杆菌介导法,以白细胞介素-4(interleukin-4,IL-4)基因转化中甘11号甘蓝。本研究对可能影响il-4转化率的外植体类型、外植体的预培养时间、农杆菌的侵染时间以及外植体与农杆菌的共培养时间进行了优化。试验结果表明:il-4基因对带1-2mm子叶柄的子叶的转化率显著高于下胚轴;带柄子叶在预培养1d、侵染3min、共培养1d时,转化效果最好,转化率达23.3%;下胚轴在预培养1-3d,侵染3~5min,共培养1-2d时,转化效果较好,转化率最高可达13.3%。经PCR检测及PCR-Southem杂交,初步证明目的基因il-4已经转入甘蓝的再生植株。转il-4基因甘蓝的PCR阳性再生植株已经开花并产生了后代。 展开更多
关键词 甘蓝(Brassica oleracea l.) il-4基因 转基因 根癌农杆菌介导
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Study on the Insecticidal and Antifeedant Activity of Extracts from Portulaca oleracea L. on Aphis sp.
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作者 梁永锋 《Agricultural Science & Technology》 CAS 2008年第4期137-140,共4页
[Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw mate... [Objective] The experiment studied on insecticidal and antifeedant action of on Portulaca oleracea L.to provide theoretical basis for developing a new botanical insecticide by taking Portulaca oleracea L.as a raw material.[Method] The insecticidal activities of the ethanol extracts and 5 kinds of extracts with petroleum ether,chloroform,ethyl acetate,n-butanol,water from Portulaca oleracea L..against Aphis sp.was studied;Leaves are using drug treatments of 5 extracts anti-feedant activity of Aphis sp.[Result] The results showed that 95% ethanol extract displayed high contact activity to Aphis sp.The corrected mortality after 48 h reached 95.4 % under the concentration of 50.00 mg·ml-1 and the insecticidal activities of 5 extracts against Aphis sp.was in the order that petroleum ether > chloroform > n-butanol > ethyl acetate >water.Five kinds of anti-feeding activity in the extract of the order for the water> ethyl acetate> n-butanol > chloroform >petroleum ether.[Conclusion] The extract from Portulaca oleracea L.has a strong contact toxicity of pesticide active on Aphis sp.substances and for a small polar compounds,the strong anti-feeding activity is a kind of larger polar compounds. 展开更多
关键词 PORTUlACA oleracea l.extract APHIS sp. Poisoned Anti-feeding activity Research
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Molecular Cloning and Sequence Analysis ofBoPGIP2 Gene from Brassica oleracea L. var. alboglabra
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作者 张弢 《Agricultural Science & Technology》 CAS 2009年第4期91-95,104,共6页
PGIP gene was obtained from Brassica oleracea L. var. alboglabra, named BoPGIP2. The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular... PGIP gene was obtained from Brassica oleracea L. var. alboglabra, named BoPGIP2. The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular mass of 37.1 kDa, interrupted by one intron of 95 bp in, length. Sequence analysis revealed that it has five potential N-giycosylation sites, two protein kinase C phosphrylation sites, five casin kinase Ⅱ phosphrylation sites and four N-myristoylation sites. The amino acids sequences alignment confirmed that ^145 LRR stucture was highly conserved in all aligned PGIP sequences. 展开更多
关键词 Brassica oleracea l. vat. alboglabra BoPGIP2 Molecular cloning Sequence analysis
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花椰菜(Brassica oleracea var.botrytis L.)凝集素的细胞凝集和糖抑制作用的研究 被引量:10
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作者 黄德棋 余萍 +2 位作者 朱苏闽 颜志森 林玉满 《福建师范大学学报(自然科学版)》 CAS CSCD 1992年第2期72-77,共6页
花椰莱(Brassica oleracea var. botrytis L.)凝集素能凝集兔、大鼠和小鼠的红细胞,其中对兔的红细胞的凝集活性最高,最低凝集浓度为0.68μg/ml。但不凝集我们所测试的其它16种红细胞。浓度为0.1mg/ml时,花椰菜凝集素也能凝集小鼠艾氏... 花椰莱(Brassica oleracea var. botrytis L.)凝集素能凝集兔、大鼠和小鼠的红细胞,其中对兔的红细胞的凝集活性最高,最低凝集浓度为0.68μg/ml。但不凝集我们所测试的其它16种红细胞。浓度为0.1mg/ml时,花椰菜凝集素也能凝集小鼠艾氏腹水瘤细胞,S_(180)肉瘤细胞,大鼠W_(256)肿瘤细胞、人的MGC_(80-3)胃癌细胞、小鼠和大鼠的脾脏淋巴细胞、骨髓细胞以及牛精子细胞,而不凝集人的Hela细胞。该凝集素对免的红细胞的凝集活性可被L—鼠李糖和D—树胶醛糖所抑制,最低抑制浓度分别为33.3m mol/L和16.7m mol/L。 展开更多
关键词 花椰菜 凝集素 糖抑制 细胞凝集
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Physiological Response of Halophyte (<i>Suaeda altissima</i>(L.) Pall.) and Glycophyte (<i>Spinacia oleracea</i>L.) to Salinity 被引量:2
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作者 Nataly R. Meychik Yuliya I. Nikolaeva Igor P. Yermakov 《American Journal of Plant Sciences》 2013年第2期427-435,共9页
We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels i... We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels in the medium (0.5 - 750 mМ). A distinctive feature of the halophyte was a high Na+ content in tissues at its low concentration in the medium (0.5 mM). In these conditions, Na+ accumulation in seablite roots was four-fold higher than in spinach roots, and Na+ content in seablite leaves was almost 20-fold higher than in spinach. Together with an increase in sodium concentration in the medium, K+ content decreased six-fold in seablite leaves, while in spinach it did not decrease so drastically. We can suppose that in the halophyte, some processes occur only in the presence of sodium, and these functions of sodium cannot be fully fulfilled by potassium. Analysis of protein and total nitrogen content in tissues shows that at high salinity, the ability to synthesize non-protein nitrogen-containing compounds increases in the halophyte and decreases in the glycophyte. Data on proline content dynamics show that its increase in tissues of spinach (salinity levels 150 and 250 mМ) and seablite (salinity levels 0.5 and 750 mМ) is an indicator of plant injury. In seablite and spinach, proline is not a major osmoregulator. Its concentration both in roots and leaves was no more than 2.5 μmol/g fresh weight. The data presented in this work concern the accumulation and distribution of Na+, Cl?, K+ and ions, as well as growth and biochemical parameters. Our data show that the development of adaptation reactions in the whole plants in the conditions of high salinity is determined by morphofunctional systems and their interaction. 展开更多
关键词 Suaeda altissima (l.) Pall. SPINACIA oleracea l. SAlINITY Ions PRAlINE Nitrogen Protein
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Transformation of insect-resistant gene into cauliflower (Brassica oleracea L.var. botrytis)
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作者 吕玲玲 雷建军 +2 位作者 宋明 李立云 曹必好 《Agricultural Science & Technology》 CAS 2004年第3期17-21,共5页
Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L... Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully. 展开更多
关键词 cauliflower (Brassica oleracea l. var. botrytis) Agrobacterium-me diated genetic transformation CpTI gene transgenic plant
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A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var.capitata L.) 被引量:1
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作者 CHEN Chen ZHUANG Mu +5 位作者 FANG Zhi-yuan WANG Qing-biao ZHANG Yang-yong LIU Yu-mei YANG Li-mei CHENG Fei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2013年第4期596-602,共7页
The dominant genic male sterility (DGMS) gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage (Brassica oleracea var. capitata L.), has been successfully applied in hybrid seed... The dominant genic male sterility (DGMS) gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage (Brassica oleracea var. capitata L.), has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants (CDMs399-3/CDMs399-3) is a laborious and time-consuming process. For marker-assisted selection (MAS) of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats (EST-SSR) and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis (BSA). By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygons male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding. 展开更多
关键词 cabbage (Brassica oleracea var. capitata l.) dominant genic male sterility (DGMS) expressed sequencetag-simple sequence repeats (EST-SSR) bulked segregant analysis (BSA) marker-assisted selection (MAS)
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Photosynthetic Excitation Pressure Causes Violaxanthin De-epoxidation in Aging Cabbage (Brassica Oleracea L.) Leaves 被引量:1
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作者 Amarendra Narayan Misra Dariusz Latowski Kazimierz Strzalka 《Journal of Life Sciences》 2011年第3期182-191,共10页
The purpose of the present studies was analysis of the age induced changes in photochemical efficiency and xanthophyils cycle pigments of the primary cabbage (Brassica oleracea L. cv. Capitata f. alba) leaves. Photo... The purpose of the present studies was analysis of the age induced changes in photochemical efficiency and xanthophyils cycle pigments of the primary cabbage (Brassica oleracea L. cv. Capitata f. alba) leaves. Photochemical efficiency of photosystem Ⅱ (PS Ⅱ) was studied by a pulse amplitude modulated chlorophyll fluorescence apparatus, chlorophyll concentration was analysis spectrophotometrically and xanthophyll cycle pigments were estimated by high-pressure liquid chromatography (HPLC). Leaf senescence was accompanied with a decrease both in chlorophylls concentration, the photochemical efficiency and rate constant for PS Ⅱ photochemistry whereas non-photochemical parameters increased. Excitation pressure (1-qP) which is a measure of relative lumen acidification increased by 1.2x in aging leaves. The maximum quantum yield of PS Ⅱ showed no significant change. The level of de-epoxidised xanthophylls increased but the concentration of mono- and di-epoxy xanthophylls decreased in aging leaves. A linear relationship between the excitation pressure and the depoxidation state of the xanthophyll cycle pigments and lutein, during the onset of senescence suggests that excitation pressure can be used as a sensor for monitoring the onset of senescence as well for the de-epoxidation state of the xanthophylls responsible for non-photochemical quenching in stressed leaves. 展开更多
关键词 Cabbage (Brassica oleracea l.) violaxanthin cycle excitation pressure senescence photosynthetic parameters.
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Genetic diversity analysis of Brassica oleracea L.by SSR
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作者 TIAN Yuan WANG Chao 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第3期202-205,共4页
SSR analysis on genetic diversity of 30 samples was carried out. Five primers selected from 36 primers were used to amplify 30 samples in this experiment, PCR products were separated by 6% polyacrylamide gel electroph... SSR analysis on genetic diversity of 30 samples was carried out. Five primers selected from 36 primers were used to amplify 30 samples in this experiment, PCR products were separated by 6% polyacrylamide gel electrophoresis, silver staining and photographed. The results of SSR were analyzed by UPGMA clustering. The results showed that a total of 21 gene alleles were detected by 5 SSR primers. The number of alleles ranged from 2 to 5 with an average of 4.2.PIC range was 0.257-0.92 1, with an average of 0.543. The average coefficient of genetic similarity of SSR markers among materials was 0.432. Some of cabbage cultivars in the experiment were divided into four groups except cultivars which come from Japan. 展开更多
关键词 Brassica oleracea l. SSR genetic diversity RElATIONSHIP
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Isolation and Expression Profiling of a CONSTANS-Like Gene and Two FLOWERING LOCUS T-Like Genes from Spinacia oleracea L.
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作者 Erika Abe Kaien Fujino +1 位作者 Kiyoshi Masuda Yube Yamaguchi 《American Journal of Plant Sciences》 2014年第26期4018-4028,共11页
Spinach (Spinacia oleracea L.) develops leaf rosettes under short-day conditions, and starts reproductive growth including bolting and flowering under long-day conditions. Japanese people prefer Oriental spinach that ... Spinach (Spinacia oleracea L.) develops leaf rosettes under short-day conditions, and starts reproductive growth including bolting and flowering under long-day conditions. Japanese people prefer Oriental spinach that bolts easily with a shorter photoperiod than European spinach. This is one of the main reasons that Oriental spinach is difficult to grow year-round. In order to understand spinach flowering mechanisms and obtain knowledge for spinach breeding, we isolated one CONSTANS-like (COL) and two FLOWERING LOCUS T (FT) homologs, which are key components of photoperiodic regulation of flowering time, from a Japanese cultivar. The expression of SoCOL1 showed diurnal rhythm with the highest expression at the end of the dark cycle. This diurnal rhythm is similar to the expression of BvCOL1 from sugar beet (Beta vulgaris), whose flower-promoting effect was observed when overexpressed in Arabidopsis. Phylogenetic analysis showed that SoCOL1 is the closest homolog of BvCOL1, suggesting that SoCOL1 is an ortholog of BvCOL1. SoFT1 and SoFT2 are closely related to BvFT1 and BvFT2, respectively. The expression of SoFT1 and SoFT2 were induced in advance of flower bud formation after changing the photoperiod, but the expression level of SoFT1 was much lower than SoFT2. Currently, we are speculating that SoFT2 is a flower-promoting factor of spinach, and that SoFT1 has a role in light signaling because the expression of SoFT1 showed a diurnal rhythm. 展开更多
关键词 CONSTANS FlOWERING lOCUS T SPINACIA oleracea l. Flower BUD Formation PHOTOPERIOD
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Optimization of ISSR-PCR System and Conditions for Portulaca oleracea L.
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作者 Zhaoyun WANG Dongchen NA 《Agricultural Biotechnology》 CAS 2017年第4期23-24,42,共3页
With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oler... With Portulaca oleracea L. as an experimental material, its total DNA was extracted by the improved CTAB method, the ISSR-PCR primers were screened, and the ISSR-PCR reaction system and reaction conditions for P. oleracea were Optimized. The results showed that there were 8 primers suitable for ISSR-PCR of P. oleracea. The optimal reaction system had a volume of 25 μl, including 2 x Taq Platinum PCR Master Mix 12.5 μl, primer 2 μl, ddH20 9.5 μl, and DNA template 1μl. The optimized ISSR-PCR of P. oleracea was started with pre-denaturation at 94 ℃ for 360 s, followed by 30 cycles of denaturation at 94 ℃ for 60 s, annealing at 54 ℃ for 60 s and extension at 72 ℃ for 90 s, and completed by extension at 72 ℃ for 300 s. 展开更多
关键词 Portulaca oleracea l. ISSR-PCR Reaction system Reaction conditions
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Effect of H_2O_2 on Growth of Collard( Brassis oleracea L. ) Seedlings Under Salt Stress
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作者 Wei LI Junjie GUO Hongyan LI 《Agricultural Biotechnology》 CAS 2019年第1期43-46,184,共5页
Collard variety( Brassis oleracea L. var. acephala f. tricolor Hort.) as a research material was treated with exogenous H_2O_2 and H_2O_2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growt... Collard variety( Brassis oleracea L. var. acephala f. tricolor Hort.) as a research material was treated with exogenous H_2O_2 and H_2O_2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined. After 6h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase( SOD),catalase( CAT) and ascorbate peroxidase( APX) in plants,were measured. The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0. 05 mmol/L exogenous H_2O_2 than in the simple salt stress treatment; and when endogenous H_2O_2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress. It is speculated that under salt stress,H_2O_2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves. 展开更多
关键词 BRASSICA oleracea l.var.acephala f.tricolor Hort. H 2O 2 Salt stress Antioxidase Gene expression
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Comparison of carotenoid,chlorophyll concentrations and their biosynthetic transcript levels in different coloured cauliflower
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作者 Fatemeh Izadpanah Katja Frede +1 位作者 Forouzandeh Soltani Susanne Baldermann 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第3期743-754,共12页
Carotenoids and chlorophylls are among the most widely distributed pigments in nature that play essential roles in the photosynthetic apparatus and confer diverse colours in plants.Among all vegetables,cauliflower(Bra... Carotenoids and chlorophylls are among the most widely distributed pigments in nature that play essential roles in the photosynthetic apparatus and confer diverse colours in plants.Among all vegetables,cauliflower(Brassica oleracea L.ssp.var.botrytis)is rich in phytochemicals and is an important crop grown all over the world.This study investigates carotenoid and chlorophyll concentrations in differently pigmented cultivars and elucidates the role of transcriptional regulation of carotenoid accumulation including lutein andβ-carotene.Here,we characterised changes in pigments by UHPLC-DAD-ToF-MS and changes in transcript levels of carotenoid metabolic genes by qRT-PCR in florets and leaves of orange(‘Jaffa'and‘Sunset'),purple(‘Di Sicilia Violetto'and‘Graffiti'),green(‘Trevi')and white(‘Clapton')cultivars.Transcript levels of all carotenoid metabolic genes showed different transcript level patterns in the leaves and florets.Compared to the other cultivars,the orange cultivars had the highest levels ofβ-carotene in the florets and lutein in the leaves resulting in changes lutein/β-carotene ratios.In the green cultivar,higher transcript levels were also found,especially for phytoene synthase and phytoene desaturase genes of the core biosynthesis pathway.However,no increased carotenoid concentrations were observed,possibly due to a higher carotenoid turnover induced by the carotenoid cleavage dioxygenase 4 in the green cultivar.In the white(‘Clapton')and purple(‘Di Sicilia Violetto'and‘Graffiti')cultivars the phytoene desaturase transcript levels as well as carotenoid concentrations were low.Chlorophyll concentrations changed in trend comparable to the carotenoid concentrations and were only significantly lower in the leaves of the orange cultivar‘Jaffa'.Also,the chlorophyll a/b ratio changed in‘Jaffa'.In florets the highest chlorophylls concentrations were observed for the green cultivar(‘Trevi')and the purple cultivar(‘Di Sicilia Violetto').Taken together,the study demonstrates the complex source-sink relationship of carotenoid accumulation in different coloured cauliflower. 展开更多
关键词 Brassica oleracea l.var.botrytis CAROTENOID lUTEIN Β-CAROTENE BIOSYNTHESIS qRT-PCR
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NaCl胁迫对马齿苋光合作用及叶绿素荧光特性的影响 被引量:20
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作者 洪立洲 王茂文 +4 位作者 丁海荣 朱小梅 刘冲 邢锦城 赵宝泉 《西北植物学报》 CAS CSCD 北大核心 2011年第12期2516-2521,共6页
以马齿苋为材料,采用温室盆栽法研究了14dNaCl胁迫处理对其幼苗生长、光合作用和叶绿素荧光特性的影响。结果显示:(1)马齿苋幼苗的鲜重和株高在25mmol.L-1 NaCl胁迫时与对照无显著差异,但其随着NaCl浓度的继续增加均显著降低,且其生物... 以马齿苋为材料,采用温室盆栽法研究了14dNaCl胁迫处理对其幼苗生长、光合作用和叶绿素荧光特性的影响。结果显示:(1)马齿苋幼苗的鲜重和株高在25mmol.L-1 NaCl胁迫时与对照无显著差异,但其随着NaCl浓度的继续增加均显著降低,且其生物量受到的抑制早于株高。(2)NaCl胁迫下,马齿苋幼苗叶片净光合速率(Pn)降低,胞间二氧化碳浓度(Ci)增大,且两者的变化幅度随着NaCl浓度增加而增大。(3)NaCl胁迫下,马齿苋幼苗叶片的初始荧光(F0)、最大荧光(Fm)、可变荧光(Fv)、恒态荧光(Fs)、恒态荧光与初始荧光差值(△F0)、PSⅡ潜在光化学效率(Fv/F0)和PSⅡ最大光化学效率(Fv/Fm)均降低,叶片光化学荧光猝灭系数(qP)也在NaCl胁迫下降低,而非光化学荧光猝灭系数(NPQ)则上升;在0~50mmol.L-1 NaCl胁迫下,幼苗叶片各荧光参数下降幅度小于其他高浓度NaCl胁迫。研究表明,在NaCl胁迫条件下,马齿苋幼苗叶片的光合作用受光抑制伤害,但在低浓度NaCl下能够较多地将光能用于光化学反应,光抑制程度较低,保持了较高的净光合速率,明显减轻盐胁迫对植株生长的影响,表现出一定的耐盐性。 展开更多
关键词 马齿苋 NACl胁迫 叶绿素荧光 光合作用
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UPLC-MS-MS法快速测定甘蓝和土壤中虱螨脲的残留量 被引量:6
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作者 彭茂民 夏虹 刘丽 《湖北农业科学》 2015年第24期6360-6363,共4页
建立了甘蓝(Brassica oleraceaL.)和土壤中虱螨脲残留的超高效液相色谱-三重四极杆串联质谱(UPLC—MS—MS)快速检测的方法。样品用酸化乙腈提取,以QuEChERS法净化,超高效液相色谱一串联质谱电喷雾负离子模式检测。结果表明,虱... 建立了甘蓝(Brassica oleraceaL.)和土壤中虱螨脲残留的超高效液相色谱-三重四极杆串联质谱(UPLC—MS—MS)快速检测的方法。样品用酸化乙腈提取,以QuEChERS法净化,超高效液相色谱一串联质谱电喷雾负离子模式检测。结果表明,虱螨脲在5-500μg/L范围内,浓度和响应峰面积线性关系良好。在0.01、0.02、0.1和0.5mg/kg4个添加浓度下,平均回收率在78.5%~113.6%.相对标准偏差(RSD)为2.5%-8.5%(n=6),虱螨脲在甘蓝和土壤中的定量限(LQD)均为0.01mg/kg。该方法适用于甘蓝和土壤中虱螨脲的检测. 展开更多
关键词 超高效液相色谱串联质谱(UPlC—MS—MS) QuEChERS法 甘蓝(Brassica oleracea l.) 土壤 螨脲
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NaCl胁迫对马齿苋幼苗生长及体内离子分布的影响 被引量:7
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作者 董静 邢锦城 +6 位作者 洪立洲 王茂文 朱小梅 刘冲 温祝桂 赵宝泉 丁海荣 《浙江农业学报》 CSCD 北大核心 2017年第2期236-243,共8页
以马齿苋幼苗为试验材料,研究了沙培条件下,0、100和200 mmol·L^(-1)NaCl处理对其生长及不同器官中Na^+、K^+、Ca^(2+)和Mg^(2+)分布的影响。结果表明:不同浓度NaCl处理下,马齿苋幼苗地上部干质量及单株叶片数均有所下降,但根系干... 以马齿苋幼苗为试验材料,研究了沙培条件下,0、100和200 mmol·L^(-1)NaCl处理对其生长及不同器官中Na^+、K^+、Ca^(2+)和Mg^(2+)分布的影响。结果表明:不同浓度NaCl处理下,马齿苋幼苗地上部干质量及单株叶片数均有所下降,但根系干质量和根冠比均有不同程度的增加。根、茎、叶中Na^+含量较对照均显著升高,且主要储存于根中;K^+含量较对照显著下降,茎中K^+含量降幅较小,而叶片中降幅较大;叶片中Ca^(2+)和Mg^(2+)含量显著高于茎和根。根、茎、叶中K^+/Na^+、Ca^(2+)/Na^+、Mg^(2+)/Na^+比值随NaCl处理浓度的增加而降低,主要原因是Na^+净增加;但叶片中K^+/Na^+、Ca^(2+)/Na^+、Mg^(2+)/Na^+比值显著高于茎和根系。随着Na Cl浓度的增加,马齿苋幼苗从根到茎的离子选择性运输能力先升高后降低,而茎到叶片的离子选择性能力总体呈升高趋势。综合分析表明,马齿苋可能通过将Na^+区域化到根系中,并增强植株向茎叶选择运输K^+、Ca^(2+)和Mg^(2+)的能力,从而缓解NaCl处理对马齿苋的伤害。 展开更多
关键词 马齿苋 NACl 离子含量
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L-精氨酸处理对青花菜贮藏过程中品质的影响 被引量:6
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作者 史君彦 王清 +1 位作者 高丽朴 杨娜 《湖北农业科学》 北大核心 2013年第22期5550-5553,共4页
试验用0.2 mmol/L的L-精氨酸(L-Arg)溶液处理青花菜(Brassica oleracea var.italica)20 min,探讨在贮藏过程中对青花菜品质的影响。结果表明,L-Arg处理可有效抑制青花菜的黄化和细胞膜透性的增加,保持可溶性固形物含量,延缓叶绿素的降... 试验用0.2 mmol/L的L-精氨酸(L-Arg)溶液处理青花菜(Brassica oleracea var.italica)20 min,探讨在贮藏过程中对青花菜品质的影响。结果表明,L-Arg处理可有效抑制青花菜的黄化和细胞膜透性的增加,保持可溶性固形物含量,延缓叶绿素的降解和维生素C的流失。L-Arg处理能够有效延缓青花菜的衰老,抑制营养物质的流失。 展开更多
关键词 l-精氨酸 青花菜(Brassica oleracea var italica) 品质
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甘蓝自交不亲和信号传导中SRK结合蛋白基因THL1的克隆与序列分析 被引量:9
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作者 刘东 朱利泉 王小佳 《园艺学报》 CAS CSCD 北大核心 2003年第1期56-58,共3页
采用PCR和RT PCR技术 ,从甘蓝基因组和柱头总RNA中扩增获得了 719bp的硫氧还蛋白(THL1)的全长基因序列和 4 30bp的cDNA序列。序列分析首次表明 ,克隆的THL1基因全序列有两个内含子 ,cDNA序列编码
关键词 信号传导 SRK结合蛋白基因 序列分析 甘蓝 硫氧还蛋白 自交不亲和 基因克隆 S位点受体激酶 THl1基因
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菠菜AFLP反应体系初探 被引量:4
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作者 梅燚 侯雷平 +2 位作者 崔艳玲 陈海丽 孟淑春 《华北农学报》 CSCD 北大核心 2010年第4期111-115,共5页
提取菠菜嫩叶DNA,运用改良CTAB法提取6份菠菜品种的DNA,对AFLP反应体系的DNA用量、酶切连接、预扩、选扩等试验条件进行了优化分析,初步建立适合于菠菜作物的AFLP反应体系。结果表明:①在PCR仪中酶切的效果比水浴的要好,酶切反应对酶切... 提取菠菜嫩叶DNA,运用改良CTAB法提取6份菠菜品种的DNA,对AFLP反应体系的DNA用量、酶切连接、预扩、选扩等试验条件进行了优化分析,初步建立适合于菠菜作物的AFLP反应体系。结果表明:①在PCR仪中酶切的效果比水浴的要好,酶切反应对酶切时间和DNA的浓度要求不敏感。②菠菜AFLP预扩选扩体系的反应体积为20μL,Mg2+(25mmol/L)1.2μL,dNTPs(2.5mmol/L)1.6μL,Taq-polymerase(5U/μL)0.2μL最佳。为菠菜AFLP分子标记的品种亲缘关系鉴定和遗传育种等提供一定的理论基础。 展开更多
关键词 菠菜 AFlP 影响因子
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