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A Genetic Linkage Map of Brassica campestris L.ssp. pekinensis (syn. B. rapa L. ssp. pekinensis) 被引量:8
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作者 YU Shuan-cang, WANG Yong-jian and ZHENG Xiao-yingBeijing Vegetable Research Center, Beijing 100089 , P. R. China 《Agricultural Sciences in China》 CAS CSCD 2003年第1期49-55,共7页
A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers inclu... A molecular genetic map of Chinese cabbage was constructed with a 102 recombinant inbred (RI) population from a cross of two cultivated Chinese cabbage lines 177 and 276, using AFLP and RAPD markers. 352 markers including 265 AFLP markers and 87 RAPD markers were integrated into 17 linkage groups. It covered a total of 2 665. 7 cM with an average interval of 7. 6 cM. AFLP marker is efficient for map construction while it easily forms clusters to cause big gaps in map. A total of 13.92 % abnormal segregation markers distributed in the map. The molecular genetic map is fundamental for gene localization, comparative genomics, and QTL mapping of important agronomic traits. 展开更多
关键词 brassica campestris l. ssp. pekinensis(syn. B. rapa l. ssp. pekinensis) Genetic linkage map AFlP RAPD
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Effect of Combined Use of Boron and Manganese Fertilizers on the Nutritional Quality and Physiological Indices of Brassica campestris L.ssp.chinensis L.var.utilis
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作者 肖伟 刘红利 +1 位作者 梁英 黄长干 《Agricultural Science & Technology》 CAS 2013年第11期1591-1593,1598,共4页
[Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutri... [Objective] The purpose of this study is to determine the effects of com-bined use of boron and manganese fertilizers on the nutritional quality and physio-logical indices of Brassica campestris. [Method] In the nutrient solutions for growing B. campestris by hydroponics, boric acid and manganese sulfate were added at 0.5, 2.5, and 7.5 mg/L respectively. Another treatment without boron and manganese was prepared as the control. Quality and physiological indices of B. campestris in the 10 treatments were measured. [Result] Boron and manganese shared obvious in-teraction in improving the quality and physiological indices of B. campestris. To cul-tivate B. campestris with high quality and strong resistance, the optimum concentra-tions of boron and manganese in the nutrient solution should be 2.5 mg/L boric acid and 2.5-7.5 mg/L manganese sulfate. [Conclusion] The findings wil provide refer-ence for studying effects of trace elements on nutrient composition of vegetables. 展开更多
关键词 Boron and manganese fertilizer brassica campestris l. ssp. chinensis l.var. utilis Quality Physiological activity
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Role of nitrification inhibitor DMPP(3,4-dimethylpyrazole phosphate) in NO^-_3-N accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil 被引量:18
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作者 XUChao WULiang-huan +1 位作者 JUXiao-tang ZHANGFu-suo 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第1期81-83,共3页
The influence of nitrification inhibitor(NI) 3,4 dimethylpyrazole phosphate(DMPP) on nitrate accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil at surface layer were investigated ... The influence of nitrification inhibitor(NI) 3,4 dimethylpyrazole phosphate(DMPP) on nitrate accumulation in greengrocery( Brassica campestris L. ssp. chinensis ) and vegetable soil at surface layer were investigated in field experiments in 2002 and 2003 Results showed that NI DMPP took no significant effect on yields of edible parts of greengrocery, but it could significantly decrease NO - 3 N concentration in greengrocery and in vegetable soil at surface layer. In addition, NI DMPP could reduce the NO - 3 N concentration during the prophase stage of storage. 展开更多
关键词 dimethylpyrazole phosphate(DMPP) NO^-_3-N vegetable soil greengrocery( brassica campestris l. ssp. chinensis )
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Effect of Nitrogen and Sulfur Supply on Glucosinolates in Brassica campestris ssp.chinensis 被引量:10
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作者 CHEN Xin-juan ZHU Zhu-jun NI Xiao-lei QIAN Qiong-qiu 《Agricultural Sciences in China》 CAS CSCD 2006年第8期603-608,共6页
Glucosinolates (GSs) are a group of plant secondary metabolites containing abundant nitrogen (N) and sulfur (S) mainly in Brassica and have the beneficial effects on human health including anti-carcinogenic, cho... Glucosinolates (GSs) are a group of plant secondary metabolites containing abundant nitrogen (N) and sulfur (S) mainly in Brassica and have the beneficial effects on human health including anti-carcinogenic, cholesterol-reducing and other pharmacological effects. The objective of this study was to investigate the effect of different concentrations of N (5, 10, and 20 mmol L-a, denoted by N5, N10 and N20) and S (0,5, 1, and 2 mmol L^-1, denoted by S0.5, S1 and S2) on the yield and GSs in pakchoi (Brassica campestris L. ssp. chinensis var. communis) in hydroponics. Results showed that N10 and N20 significantly enhanced the yield compared with N5, however, N20 had a negative effect relative to N10. Only with N10 and N20 low S supply (S0.5) reduced the yield. The concentrations of aliphatic GSs, aromatic GS and total GSs were enhanced by N5 and indolyl GSs were enhanced by N20. S2 enhanced the concentration of individual GS and total GSs. The concentrations of indolyl GSs were maximized in N20S2 treatment, whereas the highest concentrations of aliphatic GSs, aromatic GS and total GSs were found in N5S2 treatment. Effects of N and S on aliphatic GSs were higher than on indolyl GSs. The results suggest that the accumulation of aliphatic GSs and aromatic GS could be enhanced by low N and high S and restricted by high N while that of indolyl GSs could be enhanced by high N and high S. 展开更多
关键词 GlUCOSINOlATE NITROGEN SUlFUR brassica campestris l. ssp. chinensis var. communis
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Differential Expression Analysis of Genie Male Sterility A/B Lines in Chinese Cabbage-Pak-Choi (Brassica campestris ssp. chinensis Makino) 被引量:3
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作者 WANGYong-qin CAOJia-shu 《Agricultural Sciences in China》 CAS CSCD 2003年第2期195-199,共5页
To determine differential expression of genie male sterility A/B lines in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee), we used the RNA fingerprinting technique, cDNA-... To determine differential expression of genie male sterility A/B lines in Chinese cabbage-pak-choi (Brassica campestris ssp. chinensis Makino var. communis Tsen et Lee), we used the RNA fingerprinting technique, cDNA-AFLP analysis, in different developmental stages and different tissues. While no obvious differential expressions were observed in rosette leaves, florescence leaves, and scapes, some differential expressions were found in alabstrums of A/B lines and among leaves, scapes and alabstrums. We analyzed the al-abstrums collected in different developmental stages with 10 primer combinations. We got a unique band between middle size alabstrums and large alabstrums in B line in one of the ten pair primers, and in another one pair, one band reflecting a higher gene-expression level in A line than that in B line was obtained. No unique bands were found with the other primer combinations. The bands reflecting different gene-expression level were confirmed by Northern hybridization. The results indicated that cDNA-AFLP was a suitable tool for studying differential expression of genie male sterility in plants. SDS-polyacrylamide gel electrophoresis patterns of soluble proteins further verified the difference in A/B lines. 展开更多
关键词 Chinese cabbage brassica campestris l. (syn. B. rapa l.) Genie male sterility Differential expression CDNA-AFlP SDS-PAGE
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Effect of the Antisense BcMF12 Driven by the BcA9 Promoter on Gene Silencing in Brassica campestris L.ssp.chinensis 被引量:1
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作者 SONG Jiang-hua ZHANG Li-xin +1 位作者 YU Xiao-lin CAO Jia-shu 《Agricultural Sciences in China》 CAS CSCD 2008年第8期922-928,共7页
The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was am... The study analyzed the silencing of BcMF12 gene regulated by BcA9 promoter in the transgenic pakchoi and confirmed the effect of antisense BcMF12 gene on the pollen development. A conserved BcMF12 gene fragment was amplified from the cDNA of flower buds in pakchoi (Brassica campestris L. ssp. chinensis, syn. B. rapa L. ssp. chinensis) and was fused to the anther specific BcA9 promoter. The plant antisense expression vector was constructed and then introduced into pakchoi via Agrobacterium-mediated transformation. The transgenic plants were screened by antibiotics and molecular analysis. PCR and Southern blot revealed that the antisense BcMF12-GUS fusion gene regulated by BcA9 promoter was integrated into transgenic plants. Northern blot suggested that the expression of BcMF12 gene was down-regulated significantly. The pollen germination rate of transgenic plants with antisense BcMF12 gene decreased as compared with that of the control plants. The expression of the gene BcMF12 related to the pollen development was inhibited by the antisense BcMF12 driven by BcA9 promoter, which consequently affected the pollen development in pakchoi. 展开更多
关键词 brassica campestris l. ssp. chinensis BcMF12 BcA9 promoter antisense RNA gene expression GUSactivity
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大白菜(Brassica pekinensis L.)对镉富集基因型差异的研究 被引量:40
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作者 刘维涛 周启星 +1 位作者 孙约兵 于志国 《应用基础与工程科学学报》 EI CSCD 2010年第2期226-235,共10页
研究了15种大白菜基因型对镉(Cd)富集的基因型差异,以期筛选出低积累Cd的白菜基因型.盆栽试验结果表明,不同白菜基因型的Cd含量、富集系数(BFs)和转运系数(TFs)差异显著(P<0.05).丰源新3号、现代夏秋和春秋六轮的Cd积累量均小于0.50m... 研究了15种大白菜基因型对镉(Cd)富集的基因型差异,以期筛选出低积累Cd的白菜基因型.盆栽试验结果表明,不同白菜基因型的Cd含量、富集系数(BFs)和转运系数(TFs)差异显著(P<0.05).丰源新3号、现代夏秋和春秋六轮的Cd积累量均小于0.50mg/kg,其富集系数和转运系数都小于1.0,具有一定低积累Cd的潜力.大田试验的结果表明丰源新3号的Cd含量小于0.50mg/kg,富集系数和转运系数均小于1.0,表明其为排斥Cd的白菜基因型.现代夏秋和春秋六轮的富集系数和转运系数均小于1.0,但其地上部Cd含量均高于0.50mg/kg,不属于Cd低积累白菜.值得注意的.北京小杂56在盆栽和大田中的Cd含量、富集系数和转运系数均大于1.0,可用于植物修复污染土壤. 展开更多
关键词 大白菜 镉污染 基因型差异 筛选 镉低积累
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镉胁迫对小白菜(Brassica campestris L.)抗氧化机理的影响 被引量:15
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作者 刘可慧 于方明 +4 位作者 李明顺 周振明 谌斌 蓝殿 孙双玉 《生态环境》 CSCD 北大核心 2008年第4期1466-1470,共5页
采用水培的方法,研究了不同Cd2+水平(0、1、2.5、5、10、15mg·L-1)对小白菜(Brassica campestris L.)抗氧化酶(SOD、POD、CAT)活性、非酶物质(SH、GSH、PCs)含量以及生长的影响。结果表明,小白菜叶片和根系SOD活性随Cd处理质量浓... 采用水培的方法,研究了不同Cd2+水平(0、1、2.5、5、10、15mg·L-1)对小白菜(Brassica campestris L.)抗氧化酶(SOD、POD、CAT)活性、非酶物质(SH、GSH、PCs)含量以及生长的影响。结果表明,小白菜叶片和根系SOD活性随Cd处理质量浓度的增加呈降低的变化趋势,POD、CAT活性以及MDA含量随Cd处理质量浓度的增加而增加。小白菜的生物量、根长、株高、叶绿素质量分数随Cd处理质量浓度的增加显著降低(P<0.05),表明Cd抑制了小白菜的生长,破坏了叶绿素的合成。小白菜地上、地下部镉质量分数均随Cd处理质量浓度的增加而显著增加(P<0.05)。当Cd处理质量浓度为15mg·L-1,小白菜地上、地下部镉质量分数分别达到637.5、1663.0mg·kg-1,表明小白菜对Cd有良好的富集效果。小白菜根系与叶片中SH、GSH和PCs含量均随Cd处理质量浓度增加而增加的变化趋势,表明SH、GSH和PCs在解毒小白菜Cd毒害中起着重要作用。 展开更多
关键词 CD 小白菜 抗氧化酶 非酶物质
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Preliminary Study on Tissue Culture Technique of Brassica campestris L. ssp. chinensis (L.) Makino var. utilis
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作者 乔燕春 黄红弟 +3 位作者 张华 李光光 郑岩松 刘自珠 《Agricultural Science & Technology》 CAS 2015年第10期2202-2206,2213,共6页
In order to establish a rapid propagation system for Brassica campestris L. ssp. chinensis var. utilis, the anther and cotyledon-cotyledonary petioles were used as explants to conduct tissue culture research. The resu... In order to establish a rapid propagation system for Brassica campestris L. ssp. chinensis var. utilis, the anther and cotyledon-cotyledonary petioles were used as explants to conduct tissue culture research. The results showed that not bloomed flower buds with higher styles than petals were appropriate for anther cul- ture. Moreover, most microspores were at the mid-late uninucleate stage at that time. The pollen germination rates of Brassica campestris L. ssp. chinensis var. u- tills were low and the germination rates in autumn and winter were higher than that in summer. The induction medium of anther callus of Brassica campestris L. ssp. chinensis var. utilis was MS+1.0 mg/L KT+1.0 mg/L 2, 4-D+3% sugar+6 g/L agar+ 8% coconut milk and the induction media of adventitious bud were MS+2.0 mg/L 6-BA+ 0.5 mg/L NAA+I.0 g/L activated carbon+2% sugar+6 g/L agar or MS+2.0 mg/L ZT+ 0.5 mg/L IAA+0.5 g/L AgNO3+1.0 g/L activated carbon+2% sugar+6 g/L agar. The induction percentage of adventitious bud by anther culture was 36.7%. Browning phenomenon appeared during the culture of adventitious bud and regeneration plant could not be formed. The plant regeneration rate reached 80% when cotyledon- cotyledonary petioles were used as explant. 展开更多
关键词 brassica campestris l. ssp. chinensis var. utilis ANTHER Cotyledon- cotyledonary petioles Tissue culture
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Effects of Different Cadmium Levels on Active Oxygen Metabolism and H_2O_2-Scavenging System in Brassica campestris L.ssp.chinensis
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作者 SUNGuang-wen ZHUZhu-jun FANGXue-zhi 《Agricultural Sciences in China》 CAS CSCD 2004年第4期305-309,共5页
The effects of different Cd (Cadmium) levels on generation of active oxygen speceies(AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensiswere studied. The results showed that generat... The effects of different Cd (Cadmium) levels on generation of active oxygen speceies(AOS) and H2O2-scavenging system in the leaves of Brassica campestris L. ssp. chinensiswere studied. The results showed that generation rate, and H2O2 content were enhancedand malondialdehyde (MDA) content increased with the increase of Cd concentrations inthe growth medium. The activities of ascorbate peroxidase (APX), dehydroascorbatereductase (DR) and glutathione reductase (GR) were promoted by the addition of Cd.Exposed to Cd also increased the contents of ascorbate (AsA) and glutathione (GSH) in theleaves. 展开更多
关键词 brassica campestris l.ssp.chinensis Cadmium Active oxygen species Glutathione Ascorbate H2O2-scavenging enzymesO2_
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Study on Agrobacterium tumefaciens-mediated Transformation of Brassica campestris L. with Fusion Gene Ycoil-bFGF
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作者 徐岩 肖艳双 +4 位作者 杜金霞 汪洪 郑伟 李营 庞实锋 《Agricultural Science & Technology》 CAS 2009年第4期31-36,共6页
[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF wa... [ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed ( Brassica campestris L. ) by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows: explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds. 展开更多
关键词 Basic fibroblast growth factor (bFGF) Plant bioreactor Oil-body system Agrobacterium tumefaciens brassica campestris l.
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Analysis on Cloning and Expression of WRKY Gene Segment in Brassica campestris L.
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作者 宋琴 赵福宽 +1 位作者 孙清鹏 杨爱珍 《Agricultural Science & Technology》 CAS 2011年第8期1111-1115,共5页
[Objective]The aim was to explore the relative expression trends of WRKY transcription factor gene under ABA treatment in Brassica campestris.[Method]Actin gene and WRKY gene were cloned by using the homology cloning ... [Objective]The aim was to explore the relative expression trends of WRKY transcription factor gene under ABA treatment in Brassica campestris.[Method]Actin gene and WRKY gene were cloned by using the homology cloning method.The sequences of nucleic acid and amino acid were analyzed using BLAST and DNAMAN software.Relative expression trends of WRKY gene were detected by applying real-time relative quantification PCR(RT-qPCR)under ABA(100 μmol/L)treatment.[Result]A 680 bp WRKY gene segment and a 933 bp β-actin gene segment were acquired in Brassica campestris.The result of RT-qPCR analysis revealed that BcWRKY expression could be induced by ABA and that the relative expression of WRKY gene reached the peak at 1 h with ABA treatment.[Conclusion]Actin and WRKY gene in B.campestris were cloned successfully,which was proved to play an important role in ABA signal pathway. 展开更多
关键词 brassica campestris l. WRKY ClONING EXPRESSION
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Genetic linkage map of Brassica campestris L.using AFLP and RAPD markers 被引量:7
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作者 卢钢 陈杭 《Journal of Zhejiang University Science》 CSCD 2002年第5期600-605,共6页
A genetic linkage map comprised of 131 loci was constructed with an F 2 population derived from an inter subspecific cross between Brassica campestris L. ssp. chinensis cv. 'aijiaohuang' and ssp. r... A genetic linkage map comprised of 131 loci was constructed with an F 2 population derived from an inter subspecific cross between Brassica campestris L. ssp. chinensis cv. 'aijiaohuang' and ssp. rapifera cv. 'qisihai'. The genetic map included 93 RAPD loci, 36 AFLP loci and 2 morphological loci organized into 10 main linkage groups (LGs) and 2 small groups, covering 1810.9cM with average distance between adjacent markers being approximately 13.8cM. The map is suitable for identification of molecular markers linked to important agronomic traits, QTL analysis, and even for marker assisted selection in breeding programs of Chinese cabbage and turnip. 展开更多
关键词 Genetic map AFlP RAPD brassica campestris l. ssp. chinensis .
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Nitrogen Dioxide-Induced Responses in Brassica campestris Seedlings: The Role of Hydrogen Peroxide in the Modulation of Antioxidative Level and Induced Resistance 被引量:2
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作者 MA Chun-yan XU Xin +1 位作者 HAO Lin CAO Jun 《Agricultural Sciences in China》 CAS CSCD 2007年第10期1193-1200,共8页
This article investigates the responses of Brassica campestris seedlings to an acute level of nitrogen dioxide (NO2) exposure in a plant growth chamber, and examines whether pretreating plants with hydrogen peroxide... This article investigates the responses of Brassica campestris seedlings to an acute level of nitrogen dioxide (NO2) exposure in a plant growth chamber, and examines whether pretreating plants with hydrogen peroxide (H2O2) will alleviate NO2-caused injury. Twenty-eight-day-old B. campestris plants sprayed with 10 mmol L^-1 H2O2 aqueous solution (corresponding to approximate 1.0 mg H2O2 per single plant) were exposed to different concentrations of NO2 (0.25, 0.5, 1.0, and 2.0 μL L^-1, respectively) for 24 h under controlled environment. To measure the plant biomass, the plants were fumigated with the same NO2 concentrations as mentioned above for 7 h per day (8.00-15.00) for 7 days. As a control, charcoal filtered air alone was applied. Data were collected on plant biomass, total chlorophyll, photosynthetic rate, stomatal conductance, nitrate and nitrate reductase (NR), antioxidative enzymes, ascorbate (ASA), and malondialdehyde (MDA), immediately after exposure. The results showed that exposure to a moderate dose of NO2 (e.g., 0.25 μL L^-1) had a favorable effect on plants, and the dry weight of the above-ground part increased, whereas the exposure to high NO2 concentrations (e.g., 0.5 μL L^-1 or higher) caused a reduction in the plant biomass and the total chlorophyll, when compared with the control. In addition, at 0.5 μL L^-1 or higher NO2 concentrations, prominent increases in the MDA level and superoxide dismutase (SOD) and NR activities were observed. Exposure to 1 μL L^-1 and higher NO2 resulted in necroses appearing on older leaves, and an increase in catalase (CAT) activity, decrease in ASA content, increased accumulation of NO3^-, and reduction in photosynthesis, when compared with the controls. No changes were detected in stomatal conductance under NO2 fumigation. The pretreatment with 10 mmol L^-1 H2O2 alleviated significantly NO2- caused biomass decrease and photosynthetic inhibition when compared with H2O2-untreated plants. Under NO2 fumigation, further induction in SOD and CAT activities occurred in H2O2 treated plants when compared with H2O2- untreated plants. The effect of NO2 on the ASA and MDA contents was also absent in H2O2-treated plants. However, the H2O2 treatment did not alter the nitrate content and NR activity in plants under NO2 fumigation. The H2O2 treatment caused a lower rate of stomatal conductance. Taken together, these data suggest that fumigation with an acute level of NO2 causes oxidative damage to B. campestris seedlings. The H2O2 pretreatment markedly protects plants against NO2 stress and this may be associated with inducible antioxidative level. NO2 fumigation contributes, at least in part, to the enhanced levels of nitrate in B. campestris leaves. 展开更多
关键词 nitrogen dioxide hydrogen peroxide antioxidative system brassica campestris l. ssp. chinensis l.) Makino var. cammunis Tsen et lee
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Effects of Different Simple Cultivation Facilities on Yield and Quality of Over-summer Brassica campestris L. in South China 被引量:2
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作者 梁普兴 杨瑞怡 +3 位作者 李强 高祖江 张晶 梁彪 《Agricultural Science & Technology》 CAS 2017年第12期2354-2356,2377,共4页
The effects of different simple cultivation facilities on yield and quality of Brassica campestris L. were compared to explore the optimal facility type for pro- duction of B. campestris, thereby providing a technical... The effects of different simple cultivation facilities on yield and quality of Brassica campestris L. were compared to explore the optimal facility type for pro- duction of B. campestris, thereby providing a technical reference for efficient cultiva- tion of B. campestris during hot season. A total of four treatments were designed, open field cultivation (CK), ordinary anti-insect net shed cultivation, ordinary plastic film greenhouse cultivation and Japanese translucent anti-insect net shed cultivation. The results showed that compared with those in open field cultivation, the plant height, leaf area, yield, Vc content, soluble sugar content and soluble protein con- tent of B. campestris in Japanese translucent anti-insect net shed cultivation were increased by 22%, 63%, 49%, 14%, 13% and 10%, respectively, and Japanese translucent anti-insect net shed was the optimal facility type for cultivation of B. campestris in hot season. 展开更多
关键词 South China brassica campestris l. Over-summer cultivation Simplefacility
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Identifying Cytoplasmic Male Sterile Types of Chinese Cabbage(Brassica campestris L.)by Molecular Markers
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作者 Mei LAN Jingfeng HU +5 位作者 Jiajia LIU Liqin ZHANG Hongli YANG Xuezhong XU Wenyong YAO Jiangming HE 《Agricultural Biotechnology》 CAS 2018年第4期12-15,29,共5页
Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the fu... Different sterile cytoplasm types of nine cabbage cytoplasmic male sterile materials were identified by molecular marker in the study, in order to better use molecular marker to conduct the assisted breeding in the future. Genomic DNA was isolated from Chinese cabbage by CTAB method. The design of two pairs of specific primers was performed on conserved flanking region of orf138 gene in the GenBank. PCR was performed with genomic DNA of the nine Chinese cabbage materials. The bands were sequenced. The homologous comparison was conducted in NCBI, and finally, the type of sterile cytoplasm was determined. The results showed that the bands were amplified only in four Chinese cabbage male sterile materials with two pairs of specific primers PUPIl and PIII/PIV, while the other five materials did not obtain the relative bands. The result was consistent with the field sterility identification. And then four molecular markers of Chinese cabbage Ogura cytoplasmic male sterility (CMS) were obtained. After conducting a homologous comparative analysis with BLAST in GenBank, it was found that the homologous degree was 100% in specific segments of tbe tbree sterility materials (L1-CI, L3-CI and L3- F1 ) and Ogu orf138 gene (GenBank accession No. : HQ149728) of the reported broccoli Ogu CMS. The homologous degree of L1-F1 was 99% with a variation point. The type of cytoplasmic male sterility of the other five materials needed further research. Four materials of the nine were identified as the radish cytoplasmic male sterility materials and four molecular markers were obtained. 展开更多
关键词 Chinese cabbage brassica campestris l. Ogura cytoplasm male sterile Molecular marker Gemlplasm identification
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Genetic Variation and Correlation Analysis on Seed Oil Content and Protein Content of Brassica campestris L.Germplasm Resources
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作者 Entang TIAN Lufeng LI +1 位作者 Shiyan JIA Shuchun LIN 《Asian Agricultural Research》 2018年第7期61-63,共3页
The content of oil and protein in 84 germplasm materials of Brassica campestris L. from all over the country was tested. In Guiyang environment,the oil content and protein content of these materials showed abundant va... The content of oil and protein in 84 germplasm materials of Brassica campestris L. from all over the country was tested. In Guiyang environment,the oil content and protein content of these materials showed abundant variation,and were divided into three groups. Correlation analysis showed that there was a significant negative correlation between oil content and protein content of B. campestris L. seeds,and the application and formation mechanism of the negative correlation were also discussed. 展开更多
关键词 甘蓝 种质资源 蛋白质 农产品
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Effects of Nitrogen Fertilizer with Nitrification Inhibitor DMPP (3,4-Dimethylpyrazole phosphate) on Nitrate Accumulation and Quality of Cabbage(Brassica campastris L.ssp. pekinesis) 被引量:4
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作者 XUChao WULiang-huan +1 位作者 JUXiao-tang ZHANGFu-suo 《Agricultural Sciences in China》 CAS CSCD 2004年第8期622-626,共5页
To assess the effects of N fertilizer ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3;ASN] with the new nitrification inhibitor (NI) 3, 4-dimethylpyrazole phosphate (DMPP)(ASN+DMPP) on yield, nitrate accumulation, an... To assess the effects of N fertilizer ammonium sulphate nitrate [(NH4)2SO4 plus NH4NO3;ASN] with the new nitrification inhibitor (NI) 3, 4-dimethylpyrazole phosphate (DMPP)(ASN+DMPP) on yield, nitrate accumulation, and quality of cabbage (Brassica campastrisL. ssp. pekinesis), two field trials were carried out under various soil-climaticconditions in Jinhua City and Xinchang County, Zhejiang Province of China in 2002.Results showed that DMPP could increase the mean yield by+2.0tha-1 in Jinhua, +5.5tha-1 inXinchang, decrease NO3--N content by -9.4% in Jinhua, -7.3% in Xinchang and improvenutritional quality by increasing vitamin C (VC), soluble sugar, K, Fe, Zn contentssignificantly. 展开更多
关键词 Cabbage (brassica campastris l. ssp. pekinesis) Nitrogen fertilizer Yield NO3--N Quality 3 4-Dimethylpyrazole phosphate (DMPP)
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国五国六柴油车尾气颗粒物对小白菜(Brassica campestris L.)的生态毒性
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作者 闫鼎新 李霁 +4 位作者 倪红 王晓南 义家威 王攀 刘征涛 《环境科学学报》 CAS CSCD 北大核心 2024年第3期441-449,共9页
为研究柴油车尾气颗粒物对植物的毒性效应,本研究以执行国五、国六两种排放标准的柴油车为研究对象,测定其尾气颗粒物中重金属含量,并以小白菜(Brassica campestris L.)为受试植物,分析了两种颗粒物对小白菜的毒性效应.结果表明:(1)国... 为研究柴油车尾气颗粒物对植物的毒性效应,本研究以执行国五、国六两种排放标准的柴油车为研究对象,测定其尾气颗粒物中重金属含量,并以小白菜(Brassica campestris L.)为受试植物,分析了两种颗粒物对小白菜的毒性效应.结果表明:(1)国五、国六柴油车尾气颗粒物中13种重金属总含量分别为1.04 g·kg^(-1)和0.47 g·kg^(-1),国五柴油车尾气颗粒物重金属含量高于国六柴油车;(2)在两种颗粒物的高浓度胁迫下,小白菜体内叶绿素以及蛋白质的含量均出现显著降低趋势;(3)随着两种颗粒物浓度的升高,植物体内部分氧化应激的酶指标超氧化物歧化酶(SOD)、过氧化氢酶(CAT)呈先上升后下降的趋势,而过氧化物酶(POD)以及丙二醛(MDA)、谷胱甘肽(GSH)呈上升趋势,颗粒物的污染能对小白菜产生较为显著的氧化胁迫效应.(4)21 d的毒性实验后,国五、国六柴油车尾气颗粒物对小白菜鲜重的半数有效浓度(EC50)值分别为76.27 mg·L^(-1)和104.48 mg·L^(-1).综合来看,国六标准的实施有效控制了颗粒物中重金属的含量,并一定程度地降低了其对小白菜的毒性. 展开更多
关键词 柴油车尾气颗粒物 植物毒性 重金属 小白菜
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乌菜BcVIL2基因克隆及春化响应表达分析 被引量:3
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作者 桂尚枝 刘雪晴 +7 位作者 王英 唐小燕 赵龙龙 李广 吴思文 温宏伟 汪承刚 陈国户 《西南农业学报》 CSCD 北大核心 2023年第9期1843-1851,共9页
【目的】从乌菜(Brassica campestris L.)中克隆VIN3⁃LIKE 2(VIL2)基因并进行生物信息学与表达分析,为进一步研究BcVIL2基因功能奠定基础。【方法】以‘徽乌19’为试验材料,利用同源克隆等技术获得BcVIL2基因,对其序列进行蛋白分子量(MW... 【目的】从乌菜(Brassica campestris L.)中克隆VIN3⁃LIKE 2(VIL2)基因并进行生物信息学与表达分析,为进一步研究BcVIL2基因功能奠定基础。【方法】以‘徽乌19’为试验材料,利用同源克隆等技术获得BcVIL2基因,对其序列进行蛋白分子量(MW)、糖基化位点、二级结构等生物信息学分析;利用荧光定量PCR等技术分析其应答春化表达模式。【结果】BcVIL2基因ORF(Open reading fragment)全长为1296 bp,共编码431个氨基酸,属于亲水性蛋白,预测含有1个潜在的糖基化位点和59个潜在的磷酸化位点,蛋白分子量为47.91 kDa,理论等电点(pI)为8.27;其二级结构主要由α⁃螺旋与无规则卷曲组成;亚细胞定位预测其可能在细胞核中发挥作用;同源序列比对分析显示该基因与芸薹属植物VIL2基因同源性较高;亲缘进化树分析也显示该基因与白菜(B.rapa,syn.B.campestris)和芥菜(B.juncea)VIL2基因亲缘关系很近;荧光定量PCR结果表明,盛花期时BcVIL2基因在根中表达量最低,在荚中表达量最高,而叶中表达量显著低于茎和花中表达量;一月苗龄植株春化后BcVIL2基因的表达量显著低于未春化时的表达量。【结论】乌菜VIL2基因响应春化负表达,与春化反应相关基因BraA01g032910.3C、BraA05g026410.3C与BraA10g016160.3C密切相关,可能存在互作关系,本研究结果为深入分析BcVIL2基因的功能提供了良好基础。 展开更多
关键词 乌菜 VIl2 春化 基因克隆 表达分析
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