Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats...Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.展开更多
芥菜(Brassica juncea(L.)Czern et Coss)属十字花科(Cruciferae)芸薹属(Brassica)的药食两用蔬菜。该研究概述了芥菜的烃类、黄酮类、酚酸类、多糖等多种化学成分及其抗肿瘤、抗癌、抗氧化、清除自由基、降血糖、杀虫抑菌等生物活性方...芥菜(Brassica juncea(L.)Czern et Coss)属十字花科(Cruciferae)芸薹属(Brassica)的药食两用蔬菜。该研究概述了芥菜的烃类、黄酮类、酚酸类、多糖等多种化学成分及其抗肿瘤、抗癌、抗氧化、清除自由基、降血糖、杀虫抑菌等生物活性方面的研究进展,旨在为芥菜的深入研究与开发提供参考。展开更多
以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的...以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。展开更多
基金Banaras Hindu University(BHU)for providing grant to carry out study.
文摘Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.
文摘芥菜(Brassica juncea(L.)Czern et Coss)属十字花科(Cruciferae)芸薹属(Brassica)的药食两用蔬菜。该研究概述了芥菜的烃类、黄酮类、酚酸类、多糖等多种化学成分及其抗肿瘤、抗癌、抗氧化、清除自由基、降血糖、杀虫抑菌等生物活性方面的研究进展,旨在为芥菜的深入研究与开发提供参考。
文摘以茎瘤芥(Brassica juncea Coss.var.tumida Tsen et Lee)黑斑病病株为试材,对黑斑病菌5.8 S rDNA及其侧翼ITS区序列进行克隆、测序和比对分析。结果表明,5个供试病菌碱基序列同芸薹链格孢的碱基序列相似度达到99.68%,不存在大于3 bp的碱基差异;而与甘蓝链格孢和萝卜链格孢的碱基差异较明显,均存在大于3 bp的碱基差异,且存在大量的缺失片段。初步确定引起茎瘤芥黑斑病的病原菌为芸薹链格孢。
