[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was ...[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by BLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of B. juncea.展开更多
Indian mustard (Brassica juncea L.) germplasm consisting 167 accessions including one check cultivar was evaluated for qualitative and quantitative traits. The present study was conducted to investigate genetic divers...Indian mustard (Brassica juncea L.) germplasm consisting 167 accessions including one check cultivar was evaluated for qualitative and quantitative traits. The present study was conducted to investigate genetic diversity and correlation among studied genotypes of B. juncea L. based on agro-morphological at NARC, Islamabad, Pakistan. To investigate the genetic diversity based on morphological characters, data was recorded on 20 quantitative and 12 qualitative traits. The calculated data was analyzed through two complementary methods, i.e. PCA (Principal Component Analysis) and cluster analysis. Among all the studied cultivars, significant diversity was recorded for different agro-morphological characters. Among all the parameters, maximum variance was recorded for pod shattering (427.2) followed by plant height (345.6), days to 100% flowering (336.2) and main raceme length (210.0). Among all the characters, the greatest and highly significant association (0.99) was found between days to maturity 50% and days to maturity 100% followed by correlation (0.86) among days to flowering 50% and days to flowering 100%, correlation value (0.71) was calculated among leaf length and leaf width. Using cluster analysis all the genotypes were divided into five major groups. It was observed that 7 out of 20 principal components with an Eigen value of ≥1.0 calculated for 73.92% of the total diversity observed between 167 accessions of Indian mustard (B. juncea L.). The contribution of first three PCs in the total PCs was 23.25, 12.87 and 11.24, respectively. Among all the investigated accessions two genotypes 26,813 and 26,817 showed great potential for seeds/silique, 1000-seed weight and seed yield/plant, respectively, so these genotypes are recommended for future breeding programs for achieving promising results.展开更多
Correlation analysis and principal component analysis( PCA) were conducted for some agronomic traits of 139 recombinant inbred lines of Brassica juncea L. The results showed that under the environmental conditions in ...Correlation analysis and principal component analysis( PCA) were conducted for some agronomic traits of 139 recombinant inbred lines of Brassica juncea L. The results showed that under the environmental conditions in Guiyang,the flowering time,number of seeds per silique,plant height and thousand seed weight differed significantly among different lines. The results of principal component analysis showed that the lines could be classified into three groups. The results of Pearson correlation tests showed that the plant height was positively correlated with the flowering time( P < 0. 05),and the thousand seed weight was negatively correlated with the flowering time and number of seeds per silique( P < 0. 01).展开更多
Different strategies of the application of nutrients are required to overcome the adverse effects of mustard （Brassica juncea L.） in response to NaCl stress. The objective of the present study was to determine if di...Different strategies of the application of nutrients are required to overcome the adverse effects of mustard （Brassica juncea L.） in response to NaCl stress. The objective of the present study was to determine if different added levels of nitrogen （N） in growth medium could alleviate the adverse effects of salt stress on photosynthetic capacity and accumulation of osmoprotectants and nutrients. 14 days mustard seedlings of salt-sensitive （cv. Chuutki） and salt-tolerant （cv. Radha） genotypes were fed with： （i） 0 mmol L^-1 NaCl ＋ 0 mg N kg^-1 sand （control）, （ii） 90 mmol L^-1 NaCl ＋ 30 mg N kg^-1 sand, （iii） 90 mmol L^-1 NaCl ＋60 mg N kg^-1 sand, （iv） 90 mmol L^-1 NaCl ＋90 mg N kg^-1 sand and （v） 90 mmol L^-1NaCl＋ 120 mg N kg^-1 sand. Under the condition of salinity stress, N application caused a significant ameliorative effect on both genotypes with respect to growth attributes [fresh weight （FW） and dry weight （DW）] and physio-biochemical parameters [percent water content （WC）, net photosynthetic rate （PN）, stomatal conductance （gs）, total chlorophyll （Ch1）, carbonic anhydrase （CA） activity and malondialdehyde （MDA）, nitrogen （N）, potassium （K） and sodium （Na） contents, and K/Na ratio] and yield attributes （number of pods/plant, seeds/pod and seed yield/plant）. The salt-tolerant genotype exhibited maximum value for growth, physio-biochemical and yield attributes at 60 mg N kg 1 sand than that of salt-sensitive genotype. These results suggest that application of N may ameliorate most of the attributes and prove to be a physiological remedy to increase the tolerance against the ill effects of salt stress in Brassicas.展开更多
Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats...Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.展开更多
基金Supported by National Natural Science Foundation of China(31160289)Rapeseed Industry Construction Program of Department of Agriculture of Yunnan ProvinceFund for Workstation of Academician Guan Chunyun from Department of Science and Technology of Yunnan Province~~
文摘[Objective] This study was performed to screen functional genes related to the fertility conversion of thermo-sensitive genic male sterile （TGMS） lines of Brassica juncea L. [Method] A B. juncea TGMS line K121S was selected as the experimental material. The total RNAs were isolated from fertile and sterile pollens at different development stages, including mother cell stage, tetrad stage, tricellular pollen stage and maturity stage. DDRT-PCR was carried out to identify differentially expressed genes. [Result] A total of 44 differentially expressed cDNA fragments were identified with Dot blot. And seven candidate genes related to fertility conversion of K121S were screened out by BLASTN, including callose synthase gene, aldehyde dehydrogenase gene and RNA polymerase I transcription factor RRN3 gene which were differentially expressed at the transcriptional level, H＇-ATPase gene, fructose diphosphate aldolase -class I gene, teucine-rich repeat receptor-Jike serine/threonine- protein kinase gene and alkaline/neutral invertase gene, which were differentially expressed at the post-transcriptional level. [Conclusion] The results of this study will help to explain the molecular mechanism of thermo-sensitive genic male sterility of B. juncea.
文摘Indian mustard (Brassica juncea L.) germplasm consisting 167 accessions including one check cultivar was evaluated for qualitative and quantitative traits. The present study was conducted to investigate genetic diversity and correlation among studied genotypes of B. juncea L. based on agro-morphological at NARC, Islamabad, Pakistan. To investigate the genetic diversity based on morphological characters, data was recorded on 20 quantitative and 12 qualitative traits. The calculated data was analyzed through two complementary methods, i.e. PCA (Principal Component Analysis) and cluster analysis. Among all the studied cultivars, significant diversity was recorded for different agro-morphological characters. Among all the parameters, maximum variance was recorded for pod shattering (427.2) followed by plant height (345.6), days to 100% flowering (336.2) and main raceme length (210.0). Among all the characters, the greatest and highly significant association (0.99) was found between days to maturity 50% and days to maturity 100% followed by correlation (0.86) among days to flowering 50% and days to flowering 100%, correlation value (0.71) was calculated among leaf length and leaf width. Using cluster analysis all the genotypes were divided into five major groups. It was observed that 7 out of 20 principal components with an Eigen value of ≥1.0 calculated for 73.92% of the total diversity observed between 167 accessions of Indian mustard (B. juncea L.). The contribution of first three PCs in the total PCs was 23.25, 12.87 and 11.24, respectively. Among all the investigated accessions two genotypes 26,813 and 26,817 showed great potential for seeds/silique, 1000-seed weight and seed yield/plant, respectively, so these genotypes are recommended for future breeding programs for achieving promising results.
基金Supported by National Natural Science Foundation of China:QTL Mapping Combined with GWAS Strategy to Explore Major Regulation Genes for Glucosinolate Metabolism in Brassica juncea(31560422)Science and Technology Foundation of Guizhou Province of China:Positioning,Mining and Metabolic Mechanism of Major Genes Regulating Glucosinolate from Leaves and Seeds of Brassica rapa[Qian Ke He J Zi(2015)2052]+2 种基金Scientific Research Foundation for Returned Scholars,Ministry of Education:Mining of Glucosinolate-regulated Genes in Brassica napus and Analysis of Its Expression Dynamics[Jiao Wai Si Liu(2015)1098]Doctoral Talent Introduction Fund of Guizhou University:Screening of Glucosinolate Content in Brassica Napus Varieties and Development of Associated Markers[Gui Da Ren Ji He Zi(2014)14]Construction Program of Biology First-class Discipline in Guizhou(GNYL[2017]009)
文摘Correlation analysis and principal component analysis( PCA) were conducted for some agronomic traits of 139 recombinant inbred lines of Brassica juncea L. The results showed that under the environmental conditions in Guiyang,the flowering time,number of seeds per silique,plant height and thousand seed weight differed significantly among different lines. The results of principal component analysis showed that the lines could be classified into three groups. The results of Pearson correlation tests showed that the plant height was positively correlated with the flowering time( P < 0. 05),and the thousand seed weight was negatively correlated with the flowering time and number of seeds per silique( P < 0. 01).
文摘Different strategies of the application of nutrients are required to overcome the adverse effects of mustard （Brassica juncea L.） in response to NaCl stress. The objective of the present study was to determine if different added levels of nitrogen （N） in growth medium could alleviate the adverse effects of salt stress on photosynthetic capacity and accumulation of osmoprotectants and nutrients. 14 days mustard seedlings of salt-sensitive （cv. Chuutki） and salt-tolerant （cv. Radha） genotypes were fed with： （i） 0 mmol L^-1 NaCl ＋ 0 mg N kg^-1 sand （control）, （ii） 90 mmol L^-1 NaCl ＋ 30 mg N kg^-1 sand, （iii） 90 mmol L^-1 NaCl ＋60 mg N kg^-1 sand, （iv） 90 mmol L^-1 NaCl ＋90 mg N kg^-1 sand and （v） 90 mmol L^-1NaCl＋ 120 mg N kg^-1 sand. Under the condition of salinity stress, N application caused a significant ameliorative effect on both genotypes with respect to growth attributes [fresh weight （FW） and dry weight （DW）] and physio-biochemical parameters [percent water content （WC）, net photosynthetic rate （PN）, stomatal conductance （gs）, total chlorophyll （Ch1）, carbonic anhydrase （CA） activity and malondialdehyde （MDA）, nitrogen （N）, potassium （K） and sodium （Na） contents, and K/Na ratio] and yield attributes （number of pods/plant, seeds/pod and seed yield/plant）. The salt-tolerant genotype exhibited maximum value for growth, physio-biochemical and yield attributes at 60 mg N kg 1 sand than that of salt-sensitive genotype. These results suggest that application of N may ameliorate most of the attributes and prove to be a physiological remedy to increase the tolerance against the ill effects of salt stress in Brassicas.
基金Banaras Hindu University(BHU)for providing grant to carry out study.
文摘Objective:To study the bioactivity of methanolic extract of Brassica juncea on animal model of diabetes mellitus along with its effect on diabetic and metabolic parameters.Methods:Diabetes mellitus was induced in rats by injecting streptozotocin(60 mg/kg)intraperitonealy.Blood glucose was measured on day 3 by GOD-POD method to confirm the diabetes mellitus.Rats having fasting blood glucose>250 mg/d L were further selected for study and they were divided into four groups,control,control+streptozotocin,streptozotocin+metformin(75 mg/kg)and streptozotocin+extract of B.juncea(450 mg/kg).Each group consisted of six rats of either sex.Metformin and experimental extract were administered for 21 d.Triglyceride,cholesterol level were measured on day 21 by commercially available kit.Blood glucose was measured on days 7 and 21.Anti-oxidant potential was assessed by estimating extent of lipid peroxidation(LPO)by malondialdehyde(MDA),nitric oxide(NO),superoxide dismutase(SOD)and glutathione(GSH)in liver,kidney,pancreas,muscle tissues on day 21.Unpaired and paired student’s t-test was applied for statistical analysis.Results:The extract of B.juncea showed significant decrease in blood glucose level on day 21.The treatment group showed significant difference in oxidative stress by increasing SOD and GSH and decreasing LPO and NO activity on day 21.The treatment did not show statistically significant difference of cholesterol,and triglycerides level on day 21.Conclusion:The study showed anti-hyperglycemic and anti-oxidative properties of methanolic extract of B.juncea.
