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根癌农杆菌介导白细胞介素-4基因转化甘蓝(Brassica oleracea L.)研究 被引量:2
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作者 李然红 于丽杰 +1 位作者 陶雷 王雷 《分子植物育种》 CAS CSCD 2007年第1期47-53,共7页
利用根癌农杆菌介导法,以白细胞介素-4(interleukin-4,IL-4)基因转化中甘11号甘蓝。本研究对可能影响il-4转化率的外植体类型、外植体的预培养时间、农杆菌的侵染时间以及外植体与农杆菌的共培养时间进行了优化。试验结果表明:il-... 利用根癌农杆菌介导法,以白细胞介素-4(interleukin-4,IL-4)基因转化中甘11号甘蓝。本研究对可能影响il-4转化率的外植体类型、外植体的预培养时间、农杆菌的侵染时间以及外植体与农杆菌的共培养时间进行了优化。试验结果表明:il-4基因对带1-2mm子叶柄的子叶的转化率显著高于下胚轴;带柄子叶在预培养1d、侵染3min、共培养1d时,转化效果最好,转化率达23.3%;下胚轴在预培养1-3d,侵染3~5min,共培养1-2d时,转化效果较好,转化率最高可达13.3%。经PCR检测及PCR-Southem杂交,初步证明目的基因il-4已经转入甘蓝的再生植株。转il-4基因甘蓝的PCR阳性再生植株已经开花并产生了后代。 展开更多
关键词 甘蓝(brassica oleracea l.) il-4基因
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Molecular Cloning and Sequence Analysis ofBoPGIP2 Gene from Brassica oleracea L. var. alboglabra
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作者 张弢 《Agricultural Science & Technology》 CAS 2009年第4期91-95,104,共6页
PGIP gene was obtained from Brassica oleracea L. var. alboglabra, named BoPGIP2. The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular... PGIP gene was obtained from Brassica oleracea L. var. alboglabra, named BoPGIP2. The full length of BoPGIP2 gene is 1 102 bp and the exon is 993 bp which encodes a protein of 330 amino acids with a predicted molecular mass of 37.1 kDa, interrupted by one intron of 95 bp in, length. Sequence analysis revealed that it has five potential N-giycosylation sites, two protein kinase C phosphrylation sites, five casin kinase Ⅱ phosphrylation sites and four N-myristoylation sites. The amino acids sequences alignment confirmed that ^145 LRR stucture was highly conserved in all aligned PGIP sequences. 展开更多
关键词 brassica oleracea l. vat. alboglabra BoPGIP2 Molecular cloning Sequence analysis
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Photosynthetic Excitation Pressure Causes Violaxanthin De-epoxidation in Aging Cabbage (Brassica Oleracea L.) Leaves 被引量:1
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作者 Amarendra Narayan Misra Dariusz Latowski Kazimierz Strzalka 《Journal of Life Sciences》 2011年第3期182-191,共10页
The purpose of the present studies was analysis of the age induced changes in photochemical efficiency and xanthophyils cycle pigments of the primary cabbage (Brassica oleracea L. cv. Capitata f. alba) leaves. Photo... The purpose of the present studies was analysis of the age induced changes in photochemical efficiency and xanthophyils cycle pigments of the primary cabbage (Brassica oleracea L. cv. Capitata f. alba) leaves. Photochemical efficiency of photosystem Ⅱ (PS Ⅱ) was studied by a pulse amplitude modulated chlorophyll fluorescence apparatus, chlorophyll concentration was analysis spectrophotometrically and xanthophyll cycle pigments were estimated by high-pressure liquid chromatography (HPLC). Leaf senescence was accompanied with a decrease both in chlorophylls concentration, the photochemical efficiency and rate constant for PS Ⅱ photochemistry whereas non-photochemical parameters increased. Excitation pressure (1-qP) which is a measure of relative lumen acidification increased by 1.2x in aging leaves. The maximum quantum yield of PS Ⅱ showed no significant change. The level of de-epoxidised xanthophylls increased but the concentration of mono- and di-epoxy xanthophylls decreased in aging leaves. A linear relationship between the excitation pressure and the depoxidation state of the xanthophyll cycle pigments and lutein, during the onset of senescence suggests that excitation pressure can be used as a sensor for monitoring the onset of senescence as well for the de-epoxidation state of the xanthophylls responsible for non-photochemical quenching in stressed leaves. 展开更多
关键词 Cabbage brassica oleracea l.) violaxanthin cycle excitation pressure senescence photosynthetic parameters.
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Transformation of insect-resistant gene into cauliflower (Brassica oleracea L.var. botrytis)
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作者 吕玲玲 雷建军 +2 位作者 宋明 李立云 曹必好 《Agricultural Science & Technology》 CAS 2004年第3期17-21,共5页
Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L... Cowpea Trypsin Inhibitor (CpTI) gene was transferred into the cotyle dons and hypocotyls of cauliflower by Agrobacterium-mediated transformation met hod. The best selective concentration of kanamycin (kan) was 15 mg L-1. The con centration of carbencillin (carb) was 500 mg L-1. 14 transgenic cauliflower pla nts were obtained. The putative transformants were assayed by PCR and Southern b lotting analysis. The results indicated that CpTI gene was transferred into caul iflower successfully. 展开更多
关键词 cauliflower (brassica oleracea l. var. botrytis) Agrobacterium-me diated genetic transformation CpTI gene transgenic plant
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Genetic diversity analysis of Brassica oleracea L.by SSR
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作者 TIAN Yuan WANG Chao 《Journal of Northeast Agricultural University(English Edition)》 CAS 2007年第3期202-205,共4页
SSR analysis on genetic diversity of 30 samples was carried out. Five primers selected from 36 primers were used to amplify 30 samples in this experiment, PCR products were separated by 6% polyacrylamide gel electroph... SSR analysis on genetic diversity of 30 samples was carried out. Five primers selected from 36 primers were used to amplify 30 samples in this experiment, PCR products were separated by 6% polyacrylamide gel electrophoresis, silver staining and photographed. The results of SSR were analyzed by UPGMA clustering. The results showed that a total of 21 gene alleles were detected by 5 SSR primers. The number of alleles ranged from 2 to 5 with an average of 4.2.PIC range was 0.257-0.92 1, with an average of 0.543. The average coefficient of genetic similarity of SSR markers among materials was 0.432. Some of cabbage cultivars in the experiment were divided into four groups except cultivars which come from Japan. 展开更多
关键词 brassica oleracea l. SSR genetic diversity RElATIONSHIP
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Effects of Fermented Nori (<i>Pyropia yezoensis</i>) Liquid Fertilizer on Plant Growth Characteristics and Nutrient Content of Komatsuna (<i>Brassica rapa</i>L. var. Wakana Komatsuna) Cultivated in Vermiculite 被引量:1
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作者 Seinn Moh Moh Kyi Moe +3 位作者 Yasumasa Obo Shiniti Obo Aung Zaw Htwe Takeo Yamakawa 《American Journal of Plant Sciences》 2018年第8期1601-1617,共17页
We conducted plant growth experiments in microbe-free vermiculite to study the effects of four types of fermented seaweed liquid fertilizer (SLF) made from nori (Pyropia yezoensis) seaweed on the germination, plant gr... We conducted plant growth experiments in microbe-free vermiculite to study the effects of four types of fermented seaweed liquid fertilizer (SLF) made from nori (Pyropia yezoensis) seaweed on the germination, plant growth characteristics, SPAD value, and nutrient content and uptake of komatsuna (Brassica rapa L. var. wakana komatsuna). The four types of fermented nori SLF used in this study were prepared by anaerobic fermentation of unwashed nori (SLF1), aerobic fermentation of unwashed nori (SLF2), anaerobic fermentation of washed nori (SLF3), and aerobic fermentation of washed nori (SLF4). Komatsuna seeds treated with 200-, 300-, and 400-fold dilutions of SLFs exhibited improved relative germination ratios (RGRs) at 3 and 4 days after sowing (DAS). At 4 DAS, the RGRs of seeds treated with 10-, 100-, 200-, 300-, and 400-fold SLF dilutions showed no differential effect. Seeds treated with undiluted SLFs did not germinate by 4 DAS. SLF1 may promote komatsuna seed germination. The nitrogen (N), calcium, magnesium, sodium (Na), and iodine (I) contents of plants treated with SLF1 were significantly increased relative to plants treated with the other SLFs. Moreover, the I and Na contents of plants were significantly increased by foliar spray application of different dilutions of SLF1. However, SLF treatment markedly reduced the shoot dry weight compared with 1/2-strength modified Hoagland nutrient (MHN) solution, although the same amounts of N and K were applied. SPAD values of the plants treated with SLFs were significantly higher than those of plants treated with MHN. Foliar treatment with SLFs had no significant effect on plant growth, SPAD value, or uptake of nutrients (except Na) relative to the control, but the I content was increased. Plants treated with SLF1 and SLF2 exhibited the highest Na uptake. Foliar spray treatments with SLF1 resulted in the highest I contents in plants. Based on our results, SLF1 is suitable for use as a liquid fertilizer to promote germination and increase nutrient content in komatsuna. These results need to be followed up in soil experiments in the presence of microbes in the rhizosphere. 展开更多
关键词 NORI (Pyropia yezonensis) KOMATSUNA (brassica rapa l) Aerobic and Anaerobic Fermentation Basal and Foliar Application Macro-Nutrient CONTENT I CONTENT
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A Reliable Methodology for Quantitative Extraction of Fruit and Vegetable Physiological Amino Acids and Their Subsequent Analysis with Commonly Available HPLC Systems 被引量:4
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作者 Wayne W. Fish 《Food and Nutrition Sciences》 2012年第6期863-871,共9页
Many of the extraction and amino acid analysis methodologies currently employed do not provide complete analysis of all the physiological amino acids and biogenic amines. Extraction procedures frequently employ dilute... Many of the extraction and amino acid analysis methodologies currently employed do not provide complete analysis of all the physiological amino acids and biogenic amines. Extraction procedures frequently employ dilute acid which partially converts gln and asn to glu and asp. A commonly used pre-column derivatizing agent, o-phthalaldehyde, does not react with the imino acids, pro and hydroxypro. The purpose of this investigation was to integrate extraction and analysis procedures into a reliable method for measuring the complete physiological amino acid profiles of fruit and vegetables using HPLC instrumentation commonly available to most laboratories. Water extraction of ground, frozen-thawed tissues effected complete recovery of the physiological amino acids as demonstrated by spiking experiments and tissue combination experiments. HPLC of dabsyl derivatives of the free amino acids allowed their quantification in a selection of fruit and vegetables. Physiological amino acid levels were determined for peach, apple, potato, onion, tomato, bell pepper, broccoli, and seven types of cucurbits. The coefficient of variation for estimation of an amino acid level generally fell in the range of 5% to 7%. Because of marked variability in physiological amino acid content as a result of growing conditions, cultural practices, and inherent cultivar differences, comparisons of results with literature values were not possible. 展开更多
关键词 CITRUllUS lanatus CUCUMIS melo CUCURBITA pepo CUCUMIS sativus CUCURBITA foetidissima brassica oleracea Allium cepa Prunus persica Capiscum annum SOlANUM TUBEROSUM SOlANUM lycopersicum Malus domes-tica HPlC Free Amino Acids
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Genome-wide identification and analysis of cytokinin dehydrogenase/oxidase(CKX) family genes in Brassica oleracea L.reveals their involvement in response to Plasmodiophora brassicae infections 被引量:3
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作者 Mingzhao Zhu Yong Wang +6 位作者 Shujin Lu Limei Yang Mu Zhuang Yangyong Zhang Honghao Lv Zhiyuan Fang Xilin Hou 《Horticultural Plant Journal》 SCIE CSCD 2022年第1期68-80,共13页
Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase... Cytokinins are a class of phytohormones that promote cell division and differentiation and are thought to affect plant immunity to multiple pathogens.However,a comprehensive analysis of cytokinin dehydrogenase/oxidase(CKX)family genes in cabbage has not been reported.In this study,a total of 36 CKX genes were identified using a genome-wide search method.Phylogenetic analysis classified these genes into three groups.They were distributed unevenly across nine chromosomes in B.oleracea,and 15 of them did not contain any introns.The results of colinearity analysis showed that 36 CKX gene in Arabidopsis was present in several copies in the Brassica oleracea genome.An analysis of cisacting elements indicated that all genes possessed at least one stress or hormone responsive cis-acting element.A heatmap of CKX gene expression showed the patterns of expression of these genes in various tissues and organs.Three genes(Bol028363,Bol031036 and Bol018140)were relatively highly expressed in all of the investigated tissues under normal conditions,showing the expression profile of housekeeping genes.Generally,the expression patterns of CKX genes in Jingfeng 1 and Xiangan 336 were quite different under the same treatment.Notably,three genes(Bol020547,Bol028392 and Bol045724)were significantly down-regulated and up-regulated in the susceptible and resistant material,respectively,after inoculation,which may indicate their crucial roles in resistance to clubroot disease.The results provide insights for better understanding the roles of CKX genes in the B.oleracea–P.brassicae interaction. 展开更多
关键词 brassica oleracea GENOME-WIDE Cytokinin dehydrogenase/oxidase(CKX)genes ClUBROOT
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The protoplasts isolation,culture and shoots regeneration of broccoli(Brassica oleracea var.italica)
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作者 王怀名 A.Schfer-Menuhr G.Mix-Wagner 《华北农学报》 CSCD 北大核心 1993年第S2期28-34,共7页
Eight F<sub>1</sub>-hybrid cultivars of broccoli were studied.We obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one cultivar.The leavesof propagated plan... Eight F<sub>1</sub>-hybrid cultivars of broccoli were studied.We obtained cell division,celled colonies and p-calli in 5 cultivars,roots and shoots regeneration in one cultivar.The leavesof propagated plantlets in vitro were cut into 1—2mm pieces,isolated with an enzyme solutioncontaining 2% cellulase and 1%macerase on a rotary shaker(50 rpm,21℃,3h,2500 lux light),and purified with a 0.5M sucrose solution.The purified protoplasts were placed on a drop of 1%agarose.2—3 ml liquid medium was added around the agarose drops,and all of the cultures wereincubated at 25℃ under light(4000 lux)for 16 hours.3—5 days after isolation the cell divisionwas found.About 7 days after incubation 4 multicellular colonies were formed.After 3—5 wksome p-calli were developed.When the p-calli were 2—3 mm in diameter it was transferred to asolidified medium.Once they were developed to 1 cm in diameter they were transferred on a re-generation medium.About 5 months after incubation some roots and shoots grown from the calliwere 展开更多
关键词 PROTOPlASTS CUlTURE SHOOTS REGENERATION broccoli( brassica oleracea var. ITAlICA )
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花椰菜(Brassica oleracea var.botrytis)黑腐病抗性基因同源序列分离及克隆的研究 被引量:3
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作者 古瑜 赵前程 +3 位作者 刘松 王春国 孙德岭 宋文芹 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第2期62-66,共5页
通过从 NBS 保守序列设计简并引物 PCR 的方法,以花椰菜(Brassica oleracea vat.botrytis)抗、感黑腐病的近等基因系为材料,分离得到 NBS-LRR 型抗性基因同源序列,并获得1个克隆,命名为 RGA330-7.Southern 杂交表明,该片段在近等基因系... 通过从 NBS 保守序列设计简并引物 PCR 的方法,以花椰菜(Brassica oleracea vat.botrytis)抗、感黑腐病的近等基因系为材料,分离得到 NBS-LRR 型抗性基因同源序列,并获得1个克隆,命名为 RGA330-7.Southern 杂交表明,该片段在近等基因系中存在明显的多态性,且该片段在抗黑腐病基因位点至少存在3个以上类似 RGA330-7的同源拷贝.序列分析结果认为该克隆与 NBS-LRR 型抗性基因的部分 CDSs 有很高的同源性,说明该片段属于 NBS-LRR 型.系统进化分析该序列与甘蓝型油菜的2个抗病同源序列归为一类,很可能这3个不同来源的抗性基因同源序列同属于一种抗性基因家族.因此推测该序列与花椰菜抗黑腐病基因紧密相关,为进一步克隆花椰菜抗黑腐病基因提供了可靠的候选基因,对分子标记辅助抗性育种具有重要意义。 展开更多
关键词 花椰菜(brassica oleracea var.botrytis) RGAs(resistance gene analogs RGAs)
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花椰菜(Brassica oleracea var.botrytis)抗黑腐病差异表达cDNA片段的克隆及功能的初步研究 被引量:5
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作者 古瑜 毛英伟 +3 位作者 赵前程 孙德岭 刘惠静 宋文芹 《南开大学学报(自然科学版)》 CAS CSCD 北大核心 2008年第4期42-48,共7页
采用花椰菜抗黑腐病近等基因系C731(感病系)和C712(抗病系)作为材料,利用cDNA—AFLP技术,研究了花椰菜黑腐病抗性在黑腐病菌侵染和非侵染条件下基因表达的情况.获得了两个阳性克隆M2和M6.Northern杂交和点杂交进一步证明M2是组... 采用花椰菜抗黑腐病近等基因系C731(感病系)和C712(抗病系)作为材料,利用cDNA—AFLP技术,研究了花椰菜黑腐病抗性在黑腐病菌侵染和非侵染条件下基因表达的情况.获得了两个阳性克隆M2和M6.Northern杂交和点杂交进一步证明M2是组成型表达的cDNA片段,只是在病菌侵染与非侵染条件下表达丰度不同.而M6是差异表达的cDNA片段.Southern杂交表明M6 cDNA片段在花椰菜基因组中是单拷贝序列.随后的序列分析发现M6cDNA片段与拟南芥1号染色体的BACF19P19中66665~66813bp有84%同源性,该片段编码拟南芥的2A6蛋白的部分序列.推测的氨基酸序列与拟南芥的2A6蛋白部分序列有91%同源性.用H2O2作为外源分子胁迫处理的初步功能分析发现:M6cDNA片段受H2O2诱导,在诱导早期16~24h高度表达,其在叶片中的积累呈逐渐上升趋势.根据序列分析和初步的功能分析的结果推测:M6cDNA片段可能就是编码花椰菜中类2A6蛋白的部分基因片段.是参与花椰菜抗病反应信号途径的相关调控基因片段. 展开更多
关键词 花椰菜(brassica oleracea VaF.botrytis) cDNA—AFlP
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Effect of H_2O_2 on Growth of Collard( Brassis oleracea L. ) Seedlings Under Salt Stress
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作者 Wei LI Junjie GUO Hongyan LI 《Agricultural Biotechnology》 CAS 2019年第1期43-46,184,共5页
Collard variety( Brassis oleracea L. var. acephala f. tricolor Hort.) as a research material was treated with exogenous H_2O_2 and H_2O_2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growt... Collard variety( Brassis oleracea L. var. acephala f. tricolor Hort.) as a research material was treated with exogenous H_2O_2 and H_2O_2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined. After 6h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase( SOD),catalase( CAT) and ascorbate peroxidase( APX) in plants,were measured. The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0. 05 mmol/L exogenous H_2O_2 than in the simple salt stress treatment; and when endogenous H_2O_2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress. It is speculated that under salt stress,H_2O_2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves. 展开更多
关键词 brassica oleracea l.var.acephala f.tricolor Hort. H 2O 2 Salt stress Antioxidase Gene expression
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油菜(Brassicanapus L.)千粒重性状主位点组遗传分析 被引量:5
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作者 戚存扣 浦惠明 +4 位作者 张洁夫 傅寿仲 高建芹 陈新军 盖钧镒 《江苏农业学报》 CSCD 北大核心 2005年第1期1-5,共5页
应用作物数量性状QTL体系的主位点组检测方法分析不同遗传来源的 12个甘蓝型油菜(BrassicanapusL. )亲本的千粒重性状的遗传差异类型、主位点组基因型和基因效应。结果表明: 12个甘蓝型油菜亲本间千粒重性状受 3个主位点组基因控制,各... 应用作物数量性状QTL体系的主位点组检测方法分析不同遗传来源的 12个甘蓝型油菜(BrassicanapusL. )亲本的千粒重性状的遗传差异类型、主位点组基因型和基因效应。结果表明: 12个甘蓝型油菜亲本间千粒重性状受 3个主位点组基因控制,各主位点组基因效应的方向、大小不等。3个主位点组基因的加性效应 (dM )分别为0 316 3**、-0 141 9*和-0 003 7; 3个主位点组基因的显性效应(hM)分别为-0 033 7、0 184 3*和-0 212 9*。主位点组基因加性效应(dM)的和为正值;显性效应(hM)的和为负值。主位点组遗传变异百分率为 54 44%,微位点组的遗传变异百分率为 33 26%。文中讨论了亲本和亲本间杂种千粒重性状的遗传特点和改良潜力。 展开更多
关键词
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Foliar K application delays leaf senescence of winter rapeseed(Brassica napus L.) under waterlogging 被引量:1
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作者 Lin Wan Chao Hu +3 位作者 Chang Chen Liyan Zhang Ni Ma Chunlei Zhang 《Oil Crop Science》 2017年第1期1-12,共12页
To better understand waterlogging effect on leaf senescence in winter rapseed (Brassica napus L.) during flowering stage, experiments were designed to explore foliar K application influences on adverse effects of wate... To better understand waterlogging effect on leaf senescence in winter rapseed (Brassica napus L.) during flowering stage, experiments were designed to explore foliar K application influences on adverse effects of waterlogging stress. Winter rapeseed was sprayed with K after waterlogging at initial flowering stage. Results indicated that waterlogging significantly decreased leaf net photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO2 concentration (Ci) and transpiration rate (Tr). It also declined maximum quantum yield of PS II (Fv/Fm), quantum yield of electron transport (ΦPS II) and photochemical quenching (qP), but increased leaf non-photochemical quenching (NPQ) and minimal fluorescence (Fo). Interestingly, exogenous application of K significantly alleviated waterlogging-induced photosynthesis inhibition. Foliar K application increased RuBisCO activation, chlorophyll and soluble protein contents, while significantly decreased MDA content under waterlogging stress. Moreover, K supplementation improved accumulation of K+, Ca2+, Mg2+, N, Zn2+, Mn2+, Fe2+ in leaves. In general, foliar K application is effective inalleviating deleterious effects of waterlogging stress and delays leaf senescence of winter rapeseed. 展开更多
关键词 winter RAPESEED (brassica NAPUS l.) K fertilizer WATERlOGGING stress leaf SENESCENCE physiological characteristics
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Physiological Response of Halophyte (<i>Suaeda altissima</i>(L.) Pall.) and Glycophyte (<i>Spinacia oleracea</i>L.) to Salinity 被引量:2
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作者 Nataly R. Meychik Yuliya I. Nikolaeva Igor P. Yermakov 《American Journal of Plant Sciences》 2013年第2期427-435,共9页
We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels i... We have done a comparative study of ion status, growth and biochemical parameters in shoots and roots of seablite (Suaeda altissima (L.) Pall.) and spinach (Spinacia oleracea L.) grown with different salinity levels in the medium (0.5 - 750 mМ). A distinctive feature of the halophyte was a high Na+ content in tissues at its low concentration in the medium (0.5 mM). In these conditions, Na+ accumulation in seablite roots was four-fold higher than in spinach roots, and Na+ content in seablite leaves was almost 20-fold higher than in spinach. Together with an increase in sodium concentration in the medium, K+ content decreased six-fold in seablite leaves, while in spinach it did not decrease so drastically. We can suppose that in the halophyte, some processes occur only in the presence of sodium, and these functions of sodium cannot be fully fulfilled by potassium. Analysis of protein and total nitrogen content in tissues shows that at high salinity, the ability to synthesize non-protein nitrogen-containing compounds increases in the halophyte and decreases in the glycophyte. Data on proline content dynamics show that its increase in tissues of spinach (salinity levels 150 and 250 mМ) and seablite (salinity levels 0.5 and 750 mМ) is an indicator of plant injury. In seablite and spinach, proline is not a major osmoregulator. Its concentration both in roots and leaves was no more than 2.5 μmol/g fresh weight. The data presented in this work concern the accumulation and distribution of Na+, Cl?, K+ and ions, as well as growth and biochemical parameters. Our data show that the development of adaptation reactions in the whole plants in the conditions of high salinity is determined by morphofunctional systems and their interaction. 展开更多
关键词 Suaeda altissima (l.) Pall. SPINACIA oleracea l. SAlINITY Ions PRAlINE Nitrogen Protein
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Improved genome annotation of Brassica oleracea highlights the importance of alternative splicing
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作者 Yinqing Yang Lei Zhang +7 位作者 Qi Tang Lingkui Zhang Xing Li Shumin Chen Kang Zhang Ying Li Xilin Hou Feng Cheng 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第4期961-970,共10页
Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,ha... Brassica oleracea has been developed into many important crops,including cabbage,kale,cauliflower,broccoli and so on.The genome and gene annotation of cabbage(cultivar JZS),a representative morphotype of B.oleracea,has been widely used as a common reference in biological research.Although its genome assembly has been updated twice,the current gene annotation still lacks information on untranslated regions(UTRs)and alternative splicing(AS).Here,we constructed a high-quality gene annotation(JZSv3)using a full-length transcriptome acquired by nanopore sequencing,yielding a total of 59452 genes and 75684 transcripts.Additionally,we re-analyzed the previously reported transcriptome data related to the development of different tissues and cold response using JZSv3 as a reference,and found that 3843 out of 11908 differentially expressed genes(DEGs)underwent AS during the development of different tissues and 309 out of 903 cold-related genes underwent AS in response to cold stress.Meanwhile,we also identified many AS genes,including BolLHCB5 and BolHSP70,that displayed distinct expression patterns within variant transcripts of the same gene,highlighting the importance of JZSv3 as a pivotal reference for AS analysis.Overall,JZSv3 provides a valuable resource for exploring gene function,especially for obtaining a deeper understanding of AS regulation mechanisms. 展开更多
关键词 brassica oleracea Oxford nanopore technologies Gene annotation Alternative splicing
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甘蓝型油菜BnaSLY1基因进化分析及功能研究
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作者 李嘉欣 黄莹莹 +7 位作者 吴潞梅 赵伦 易斌 马朝芝 涂金星 沈金雄 傅廷栋 文静 《作物学报》 CAS 北大核心 2025年第1期44-57,共14页
赤霉素调控植物表皮细胞增长、茎叶伸长以及株型建成。拟南芥SLY1属于F-box蛋白,它通过靶向泛素化赤霉素信号转导路径的负向调控因子——DELLA蛋白来影响植物生长发育,然而油菜中BnaSLY1的基因功能尚未揭示。本研究对BnaSLY1进行了表达... 赤霉素调控植物表皮细胞增长、茎叶伸长以及株型建成。拟南芥SLY1属于F-box蛋白,它通过靶向泛素化赤霉素信号转导路径的负向调控因子——DELLA蛋白来影响植物生长发育,然而油菜中BnaSLY1的基因功能尚未揭示。本研究对BnaSLY1进行了表达特征和进化树分析,利用CRISPR/Cas9技术创制了BnaSLY1不同拷贝数的突变体,结合RNA-Seq技术对BnaSLY1的生物学功能及其对油菜生长发育的影响进行了研究。结果表明,甘蓝型油菜Westar中有2个SLY1同源拷贝BnaA01.SLY1和BnaA06.SLY1,它们表达模式基本相同,为组成型表达基因,其蛋白定位在细胞核,且在不同的油菜品种及十字花科植物间序列保守。与对照相比,单突bnaa01sly1和bnaa06sly1开花时间推迟,株高显著降低,而双突bnasly1还表现出深绿色光叶表型,叶片厚度增加,开花期比单突进一步推迟,株高也进一步降低。RNA-Seq结果显示,双突与Westar之间的差异表达基因显著富集在生长素信号转导路径以及蜡质合成通路,多个开花时间相关基因表达也发生显著变化。本研究表明, BnaSLY1除影响植物株高和开花时间等生长发育进程,还影响表皮蜡质合成,为探索赤霉素信号转导路径在甘蓝型油菜生长发育中的重要作用奠定了理论基础。 展开更多
关键词 BnaSlY1 RNA-SEQ
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Physiological and molecular responses to cold stress in rapeseed(Brassica napus L.) 被引量:9
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作者 YAN Lei Tariq Shah +3 位作者 CHENG Yong Lü Yan ZHANG Xue-kun ZOU Xi-ling 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第12期2742-2752,共11页
Low temperature is one of the most important abiotic factors inhibiting growth, productivity, and distribution of rapeseed(Brassica napus L.). Therefore, it is important to identify and cultivate cold-tolerant germpla... Low temperature is one of the most important abiotic factors inhibiting growth, productivity, and distribution of rapeseed(Brassica napus L.). Therefore, it is important to identify and cultivate cold-tolerant germplasm. The objective of this study was to figure out the mechanism of chilling(4 and 2°C) and freezing(–2 and –4°C) stresses along with a control(22°C) in B. napus cultivars(1801 and C20) under controlled environment(growth chamber). The experiment was arranged in a complete randomized design with three replications. Our results exhibited that under chilling and freezing stresses, the increment of proline accumulation, soluble sugar and protein contents, and antioxidant enzyme activity were enhanced more in 1801 cultivar compared with C20 cultivar. At –2 and –4°C, the seedlings of C20 cultivar died completely compared with 1801 cultivar. Hydrogen peroxide(H2 O2) and malondialdehyde contents(MDA) increased in both cultivars, but when the temperature was decreased up to –2 and –4°C, the MDA and H2 O2 contents continuously dropped in 1801 cultivar. Moreover, we found that leaf abscisic acid(ABA) was enhanced in 1801 cultivar under chilling and freezing stresses. Additionally, the transcriptional regulations of cold-tolerant genes(COLD1, CBF4, COR6.6, COR15, and COR25) were also determined using real-time quantitative PCR(RT-q PCR). RT-q PCR showed that higher expression of these genes were found in 1801 as compared to C20 under cold stress(chilling and freezing stresses). Therefore, it is concluded from this experiment that 1801 cultivar has a higher ability to respond to cold stress(chilling and freezing stresses) by maintaining hormonal, antioxidative, and osmotic activity along with gene transcription process than C20. The result of this study will provide a solid foundation for understanding physiological and molecular mechanisms of cold stress signaling in rapeseed(B. napus). 展开更多
关键词 brassica NAPUS l. cold stress MORPHOlOGICAl features molecular regulation PHYSIOlOGICAl INDICATORS
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Identi cation of seed heat tolerance and its correlation with agronomic traits in Brassica napus L.
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作者 Guizhen Gao Kun Xu +5 位作者 Biyun Chen Xi Cheng Guixin Yan Jiangwei Qiao Qian Huang Xiaoming Wu 《Oil Crop Science》 2018年第1期21-32,共12页
To better understand the effect of high temperature on seed viability, artificial aging treatments were implemented on 472 accessions of rapeseed (Brassica napus L.) by constant high temperature at 70℃for 8h. Results... To better understand the effect of high temperature on seed viability, artificial aging treatments were implemented on 472 accessions of rapeseed (Brassica napus L.) by constant high temperature at 70℃for 8h. Results showed a remarkable impact of constant heat on seed germination. After heat treatment, considerable variation was found in seed germinability, some genotypes even lost the ability to germinate. The effect of accelerated aging was highly significant. Germination parameters varied among ecotypes. Seed viability varied with different origin, and was significantly and positively correlated with seed yield per plant and dry weight of aboveground parts per plant among 14 agronomic traits. Germination traits were positively correlated with oil contents. 展开更多
关键词 brassica NAPUS l. SEED VIABIlITY SEED germination heat stress AGRONOMIC traits yield quality
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QTL mapping for yield components of Brassica napus L. using double haploid and immortalized F_2 populations
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作者 Hui Wang Wenhui Huang +6 位作者 Jun Wang Jia Liu Wenxiang Wang Li Fu QAMAR U Zaman Qiong Hu Desheng Mei 《Oil Crop Science》 2018年第4期203-214,共12页
Effective silique number per plant(ESN), seeds per silique(SS), thousand seeds weight(TSW), silique length(SL) and silique density(SD) are important seed yield potential determinant traits in rapeseed(Brassica napus L... Effective silique number per plant(ESN), seeds per silique(SS), thousand seeds weight(TSW), silique length(SL) and silique density(SD) are important seed yield potential determinant traits in rapeseed(Brassica napus L.), which are controlled by quantitative trait loci(QTL). Mapping QTL to delimited chromosomal region offers an effective method for genetic dissection of these traits. A set of 96 double haploid(DH) lines were developed by crossing 2 Brassica napus lines R1 and R2, and an immortalized F_2(IF_2) population containing 124 combinations was developed by crossing those DH lines. DH populations were planted at 2 locations for 2 years and IF_2 populations were planted in 2 locations for 1 year. Based on the established 2,217.2 cM length high density genetic map, 42 QTLs were identified, with 26 QTLs detected repeatedly in different environments or populations, including 8 for SL, 7 for TSW, 4 for ESN, 4 for SS and 3 for SD. Among these identified QTLs, 3, 4, 1, 1 and 3 QTLs were considered as major QTLs for SL, TSW, ESN, SS and SD, respectively. In addition, 2 QTLs on A9 chromosome which control multiple traits were identified. These results warrant further study of fine mapping for yield and yield components. 展开更多
关键词 brassica NAPUS l. quantitative TRAIT locus(QTl) single NUClEOTIDE polymorphism(SNP) yield component traits
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