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QTL Mapping of Vernalization Requirement in Brassica rapa
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作者 Yating LIU Lei ZHANG +3 位作者 Xiaohong JI Xixi LV Yugang WANG Hui FENG 《Agricultural Biotechnology》 CAS 2015年第4期1-5,共5页
An F2 population, derived from a cross between the vernalization independent genotype ' RcBr' and the vernalization dependent genotype'08A061 ' , was developed to eonstruet a linkage map with 165 SSR and InDel mar... An F2 population, derived from a cross between the vernalization independent genotype ' RcBr' and the vernalization dependent genotype'08A061 ' , was developed to eonstruet a linkage map with 165 SSR and InDel markers. QTL analysis was performed by two phenotypie evaluation ( days to 5 em elongate stalk and flowering time) based on the difference in F2:3 families under vernalization and no vernalization. The results showed that the vernalization requirement was reeessive in Brassica rapa. Seven QTLs that controlled vernalization requirement were detected on A02 and A06 linkage groups, which explained phenotypic variation ranging from 0.08% to 22.52%. Two QTLs ( VR-DE01, VR-b391 ) were detected on the top of A02, which explained 22.52% and 14. 54% of the phenotypic variation, respectively, and closely linked with BrFLC2 gene. Two QTLs (VR-DE03, VR-DE04) were deteeted on A06 with 13.30% and 13.64% of phenotypie variation. These detected QTLs will provide useful information on understanding the genetic basis between vernalization requirement and bolting, and that will be useful for marker-assisted selection (MAS) in a breeding program for bolting resistant eultivars. 展开更多
关键词 brassica rapa vernalization requirement quantitative trait locus (qtl
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白菜春化需要的QTL定位分析 被引量:2
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作者 张磊 纪小红 +3 位作者 吕茜茜 刘亚婷 王玉刚 冯辉 《分子植物育种》 CAS CSCD 北大核心 2014年第4期726-732,共7页
本研究以一年生(春化不需要型)白菜型油菜自交系RcBr与二年生(春化需要型)大白菜高代自交系08A061为亲本材料,在春化和未春化2种不同春化处理下,调查F2:3家系薹长5cm时间和开花时间的差异,对春化需要性状进行QTL定位和分析。结果表... 本研究以一年生(春化不需要型)白菜型油菜自交系RcBr与二年生(春化需要型)大白菜高代自交系08A061为亲本材料,在春化和未春化2种不同春化处理下,调查F2:3家系薹长5cm时间和开花时间的差异,对春化需要性状进行QTL定位和分析。结果表明,春化需要为隐性性状,两种表型测量方法共检测到7个与春化需要相关的QTLs,分布于A02和A06连锁群上,各QTL可解释的表型变异在0.08%-22.52%之间。其中在A02连锁群顶部重复检测到VR-DE01和VR-FT01,表型变异贡献率分别为22.52%和14.54%,均与BrFLC2基因紧密连锁。在A06连锁群上检测到VR-DE03和VR-DE04,解释表型变异分别为13.30%和13.64%。这些QTL将为研究白菜的春化需要与抽薹的遗传关系以及晚抽薹品种的分子辅助选育奠定理论基础。 展开更多
关键词 白菜 春化需要 数量性状位点(quantitative trait locus qtl)
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