Construction of a RNA Interference Vector of Brassica napus L.Pyruvate Kinase Gene

A predominantly expressed gene, pyruvate kinease （PK） gene, control ing oil accumulation, had been identified in previous study. To construct a PK RNAi vector, a 498-bp target PK gene sequence was amplified and transferred into the pEASY-T1vector. Subsequently, the target DNA fragments were cut off by enzymes Not I and Xho I and directional y inserted into plant RNAi platform vector pHurricane, a newly developed RNAi vector in our lab, to form the PK inverse repeats. The PK inverted repeats fragment was then cloned into a modified vector pCAMBIA1390, driven by a rapeseed seed-specific promoter napin. Restriction enzyme digestion verified the successful construction of RNA interference vector. The PK RNAi con-struction wil lay a foundation for study in the function of PK in oil accumulation and metabolism in rapeseeds.

氮磷配施对麦后复种饲料油菜农艺性状及产量的影响

为探讨适宜饲料油菜(Brassica napus)生产的氮磷用量,研究氮磷施用量对麦后复种饲料油菜生长性状及其产量的影响。以华油杂62号为试验材料,采用双因素三水平随机区组设计,设置3个施氮水平(纯N1=75 kg/hm^2、N2=150 kg/hm^2、N3=225 kg/hm^2),3个施磷水平(P1=60 kg/hm^2、P2=120 kg/hm^2、P3=180 kg/hm^2)。结果表明,氮肥施用量在150～225 kg/hm^2时,饲料油菜的农艺性状和产量均有显著改善和提高。不同施磷量对饲料油菜的农艺性状和产量的影响均不显著。不同氮磷组合对饲料油菜生物产量、肥料经济效益影响明显,N2P1处理产量达94 016 kg/hm^2,产投比最高(12.68);N2P2处理的饲料油菜产量最高(100 832 kg/hm^2),产投比居中,而净收益最高。因此在饲料油菜生育期内配施N 150 kg/hm^2、P120 kg/hm^2的肥料用量,能使饲料油菜获得高产,节约肥料成本,增加经济收益。

利用正交设计优化甘蓝型油菜高频再生体系

采用L16(45)正交试验比较了BAP、NAA、AgNO3、蔗糖和外植体等5因素对甘蓝型油菜离体再生的影响,结果表明:BAP为甘蓝型油菜再生频率最主要的影响因素,其次依次为外植体、NAA和AgNO3,蔗糖对其影响不显著。结果显示,MS+BAP4mg/L+NAA0.3mg/L+AgNO32.5mg/L+2%蔗糖+0.8%琼脂为培养基,以带柄子叶为外植体的甘蓝型油菜再生体系的再生频率最高,达到94.45%。