For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from t...For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from the slope of the outtflow rate, The cell cycling time and the durations of G1 phase and S phase can be calculated from the measured fractions of G1, S cells and the cell age distribution theory. The method described here was simple and only about 1 hour was needed Several cell lines were measured and the obtained results were equivalent to the values determined by the FLSm curve or other methods.展开更多
前言已经证明X线诱导染色体畸变比诱导姐妹染色单体互换有效得多(Perry and Evans 1975,Pont et al,1976),低水平电离辐射所致损害的检测已在体外使用畸变作为一种损害标志成功地进行了研究(Kucerováet al.1972,Luchnik and Sevatt...前言已经证明X线诱导染色体畸变比诱导姐妹染色单体互换有效得多(Perry and Evans 1975,Pont et al,1976),低水平电离辐射所致损害的检测已在体外使用畸变作为一种损害标志成功地进行了研究(Kucerováet al.1972,Luchnik and Sevattkaev1976,Pant et al,1976,Schmickel 1967)。在1978年莫斯科第十四届国际遗传学会议上,Serra等介绍了一篇论文,报告了持续接受小剂量照射的受试组人群的每个淋巴细胞的姐妹染色单体互换平均数显著增加。Serra等人过去关于淋巴细胞体外急性X线照射不使SCE频率增加的报导是出人预料的。但是如果他们的发现被证实了。展开更多
文摘For any cultured cell lines during their exponentially growing phase,the outflow rate of G2M cells can be measured with bromodeoxyuridine (BrdUrd) continuous labeling.The duration of G2M phase can be estibmated from the slope of the outtflow rate, The cell cycling time and the durations of G1 phase and S phase can be calculated from the measured fractions of G1, S cells and the cell age distribution theory. The method described here was simple and only about 1 hour was needed Several cell lines were measured and the obtained results were equivalent to the values determined by the FLSm curve or other methods.
文摘前言已经证明X线诱导染色体畸变比诱导姐妹染色单体互换有效得多(Perry and Evans 1975,Pont et al,1976),低水平电离辐射所致损害的检测已在体外使用畸变作为一种损害标志成功地进行了研究(Kucerováet al.1972,Luchnik and Sevattkaev1976,Pant et al,1976,Schmickel 1967)。在1978年莫斯科第十四届国际遗传学会议上,Serra等介绍了一篇论文,报告了持续接受小剂量照射的受试组人群的每个淋巴细胞的姐妹染色单体互换平均数显著增加。Serra等人过去关于淋巴细胞体外急性X线照射不使SCE频率增加的报导是出人预料的。但是如果他们的发现被证实了。
