OBJECTIVE To investigate the expression of the RECK gene in human breast （cancer） cell lines, and to determine the relationship between RECK gene expression and the invasive capacity of the breast cancer cell lines....OBJECTIVE To investigate the expression of the RECK gene in human breast （cancer） cell lines, and to determine the relationship between RECK gene expression and the invasive capacity of the breast cancer cell lines.METHODS The invasive capacity of breast （cancer） cell lines including HBL-100, MCF-7 and MDA-MB-435S were determined by the Tran- swell method. The protein expression levels of RECK, MMP-2 and MMP- 9 genes in these three cell lines were measured by immunocytochemical methods. The expressions of the RECK gene and protein level were measured hv RT-PCR and Westrn hints in the cell lines respectively.RESULTS The order of the nvasive capacity of the breast （cancer） cell lines was MDA-MB-435S, being the highest, and HBL-100, being the lowest. The invasive capacity difference between any two groups among the three groups was significant （P〈0.01）. The protein expression level of the RECK gene in the HBL-100 cell line was highest, and no expression was detected in MDA-MB-435S cells. Moreover, the expression of the RECK gene was negatively correlated with the expression of the MMP-2 and MMP-9 genes. The mRNA level of the RECK gene in HBL-100 cells was the highest, but no expression was found in the MDA-MB-435S cells （P〈0.001）.CONCLUSION There was a significant negative correlation between the expression level of the RECK gene and invasive capacity in vitro, and the RECK gene expression showed an inverse proportion to that of the MMP-2, MMP-9 genes.展开更多
Objective: To investigate the correlativity between mammographic features and c-erbB-2 of breast cancer. Meth- ods: The mammographic features of 165 patients, including calcification, distinct, esion concentration, br...Objective: To investigate the correlativity between mammographic features and c-erbB-2 of breast cancer. Meth- ods: The mammographic features of 165 patients, including calcification, distinct, esion concentration, breast cysitic hyperplasia accompanied, were studied comparatively with c-erbB-2 gene stained with immunohistochemical technique. Results: Of 165 cases, calcification impression was 84 cases (50.91%), indistinct 80 cases (48.40%), lesions were concentrated of 87 case (52.73%), accompanied breast cysitic hyperplasia 85 cases (51.52%). Conclusion: Mammographic features of breast cancer could show the status of c-erbB-2, the positive chance is higher with calcification, indistinct, lesion concentration and accompa- nied breast cysitic hyperplasia.展开更多
Scirrhous carcinoma is charactertzed by reinarkable amount of collagen fibrils, mainly type I and type III collagens. The origin of collagens is still under debate.cDNA fragments of type I and type III procollagens ...Scirrhous carcinoma is charactertzed by reinarkable amount of collagen fibrils, mainly type I and type III collagens. The origin of collagens is still under debate.cDNA fragments of type I and type III procollagens were subcloned into Gemini pGEM vectors to synthesize the 35Slabeled cRNA probes. By in situ hybridization, we have found the fibroblasts surrounding the tumor cells and cords contained abundent type I and type III procollagen mRNAs which decreased with the distance of fibroblasts from the tumor cells. In all freshly prepared tissues, the tumor cells also contained significant pro α1 (I) and pro α1 (III) mRNAs, but no or little pro α2 (I) mRNA. The results indicated that type I and type III collagens in human scirrhous carcinoma of breast are mainly produced by fibroblasts. Tumor cells also perticipate in the disposition of collagen fibrils, probably type I trimer and type III collagens in accordance with what was observed in biochemical展开更多
文摘OBJECTIVE To investigate the expression of the RECK gene in human breast （cancer） cell lines, and to determine the relationship between RECK gene expression and the invasive capacity of the breast cancer cell lines.METHODS The invasive capacity of breast （cancer） cell lines including HBL-100, MCF-7 and MDA-MB-435S were determined by the Tran- swell method. The protein expression levels of RECK, MMP-2 and MMP- 9 genes in these three cell lines were measured by immunocytochemical methods. The expressions of the RECK gene and protein level were measured hv RT-PCR and Westrn hints in the cell lines respectively.RESULTS The order of the nvasive capacity of the breast （cancer） cell lines was MDA-MB-435S, being the highest, and HBL-100, being the lowest. The invasive capacity difference between any two groups among the three groups was significant （P〈0.01）. The protein expression level of the RECK gene in the HBL-100 cell line was highest, and no expression was detected in MDA-MB-435S cells. Moreover, the expression of the RECK gene was negatively correlated with the expression of the MMP-2 and MMP-9 genes. The mRNA level of the RECK gene in HBL-100 cells was the highest, but no expression was found in the MDA-MB-435S cells （P〈0.001）.CONCLUSION There was a significant negative correlation between the expression level of the RECK gene and invasive capacity in vitro, and the RECK gene expression showed an inverse proportion to that of the MMP-2, MMP-9 genes.
基金Supported by a grant from the Science and Technology Bureau, Shen-zhen City (No. 200404025).
文摘Objective: To investigate the correlativity between mammographic features and c-erbB-2 of breast cancer. Meth- ods: The mammographic features of 165 patients, including calcification, distinct, esion concentration, breast cysitic hyperplasia accompanied, were studied comparatively with c-erbB-2 gene stained with immunohistochemical technique. Results: Of 165 cases, calcification impression was 84 cases (50.91%), indistinct 80 cases (48.40%), lesions were concentrated of 87 case (52.73%), accompanied breast cysitic hyperplasia 85 cases (51.52%). Conclusion: Mammographic features of breast cancer could show the status of c-erbB-2, the positive chance is higher with calcification, indistinct, lesion concentration and accompa- nied breast cysitic hyperplasia.
文摘Scirrhous carcinoma is charactertzed by reinarkable amount of collagen fibrils, mainly type I and type III collagens. The origin of collagens is still under debate.cDNA fragments of type I and type III procollagens were subcloned into Gemini pGEM vectors to synthesize the 35Slabeled cRNA probes. By in situ hybridization, we have found the fibroblasts surrounding the tumor cells and cords contained abundent type I and type III procollagen mRNAs which decreased with the distance of fibroblasts from the tumor cells. In all freshly prepared tissues, the tumor cells also contained significant pro α1 (I) and pro α1 (III) mRNAs, but no or little pro α2 (I) mRNA. The results indicated that type I and type III collagens in human scirrhous carcinoma of breast are mainly produced by fibroblasts. Tumor cells also perticipate in the disposition of collagen fibrils, probably type I trimer and type III collagens in accordance with what was observed in biochemical
