Background Artificial insemination(AI)is a routine breeding technology in animal reproduction.Nevertheless,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI.In this ...Background Artificial insemination(AI)is a routine breeding technology in animal reproduction.Nevertheless,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI.In this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery vehicles.In this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during transport.Results With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in AndromedR.Samples containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 h.Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.Conclusions The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs.In view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.展开更多
As a traditional precious Chinese herbal medicine,Anoectochilus roxburghii has the cooling and detoxifying effects,and can nourish yin to reduce pathogenic fire,and has anti-inflammatory and analgesic effects. In this...As a traditional precious Chinese herbal medicine,Anoectochilus roxburghii has the cooling and detoxifying effects,and can nourish yin to reduce pathogenic fire,and has anti-inflammatory and analgesic effects. In this paper,we summarized the research progress in detail about the biological characteristics,resource distribution,composition,medicinal value and rapid seedling breeding technology concerning the rare Chinese herbal medicine A. roxburghii,in order to provide a reference for the in-depth study and rational development and utilization of A.roxburghii.展开更多
The breeding technology of sunflower inbred lines with four generations in one year was explored through the research on the biological characteristics of sunflower itself and the breeding status of sunflower inbred l...The breeding technology of sunflower inbred lines with four generations in one year was explored through the research on the biological characteristics of sunflower itself and the breeding status of sunflower inbred lines using the young embryo culture technology,the greenhouse seedling technology and field test planting,based on sunflower field breeding and planting combined with greenhouse planting.The reasonable selection of planting sites as the core of the breeding technology of sunflower inbred lines with four generations in one year is a guarantee.Embryos can be taken 7-10 d after pollination to enter the next generation of cultivation,thus shortening the generational cultivation cycle.The establishment of a breeding method of sunflower inbred lines with four generations in one year reduces the cost of breeding and has a practical role and significance for the field of sunflower inbred line breeding technologies.展开更多
Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,an...Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,and results in a high yield of young abalones after being applied.展开更多
The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic m...The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic minerals, and the biotreatment for minewaste reclamation. The application of microbial technology to mineralprocess- ing has shown the advantages of high efficiency, low en-Ergy and reagent consumption, low capital and operating Costs, andlow pollution of the environment.展开更多
Food shortages arise more frequently owing to unpredictable crop yield losses caused by biotic and abiotic stresses. With advances in molecular biology and marker technology, a new era of molecular breeding has emerge...Food shortages arise more frequently owing to unpredictable crop yield losses caused by biotic and abiotic stresses. With advances in molecular biology and marker technology, a new era of molecular breeding has emerged that has greatly accelerated the pace of plant breeding. High-throughput genotyping technology and phenotyping platforms have enabled large-scale marker-trait association analysis, such as genome-wide association studies, to precisely dissect the genetic architecture of plant traits. Large-scale mapping of agronomically important quantitative trait loci, gene cloning and characterization, mining of elite alleles/haplotypes, exploitation of natural variations, and genomic selection have paved the way towards genomics-assisted breeding(GAB). With the availability of more and more informative genomic datasets, GAB would become a promising technique to expedite the breeding cycle for crop improvement.展开更多
To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new b...To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new big-ear maize variety Guidan 903 with high yield,stable yield and good quality was established by the Maize Research Institute of Guangxi Academy of Agricultural Sciences with two self-selected inbred line GRL17349 and GRL21191 as female and male parents seperately.The average yield of this variety were 7429.5 and 7000.5 kg/hm^(2)in Guangxi Maize Regional Trial and Production Test respectively,which increased by 6.3%and 5.6%than the control(Guidan162).It was approved by Guangxi Zhuang Autonomous Region Crop Variety Certification Committee in June 2020(Approval No.Gui sheng yu 2020083).Determined by the Cereal Quality Supervision and Testing Center of Ministry of Agriculture and Rural Affairs(Bejjing),the grain bulk density,ratio of crude protein,crude fat,crude starch and lysine of Guidan 903 were 782 g/L,9.41%,6.19%,71.45%,0.35%respectively.This variety has the characterisics of strong resistance to collapse and disease,good quality,high and stable yield and wide adaptability.Furthermore,its seed yield is high with easy production technology and low cost.展开更多
The artificial breeding technology for juvenile of Whitmania pigra was introduced in the paper,including selection of sites and water quality,construction of spawning pool,hatching pool and escape proof facilities,key...The artificial breeding technology for juvenile of Whitmania pigra was introduced in the paper,including selection of sites and water quality,construction of spawning pool,hatching pool and escape proof facilities,key technology of leech selection,feeding,cocoon hatching,juvenile feeding and management.展开更多
Cangmai 028 was selected and bred according to the climatic characteristic of the winter wheat in Heilonggang Basin taking 98(083)as the female parent and the Canghe 030 as the male parent by "two-row parallel al...Cangmai 028 was selected and bred according to the climatic characteristic of the winter wheat in Heilonggang Basin taking 98(083)as the female parent and the Canghe 030 as the male parent by "two-row parallel alternate selection" method.Cangmai 028 has characteristics of high resistance,water-saving,high and stable yield,and rich genetic background.This study elaborated selection and breeding background and methods,characteristics,quality,and yield of Cangmai 028.展开更多
The breeder’s seeds of 56 A are produced through individual selection,sib mating in pairs and selection in isolation zone(net) plant line nursery.The foundation seeds are produced by isolated propagation of the bree...The breeder’s seeds of 56 A are produced through individual selection,sib mating in pairs and selection in isolation zone(net) plant line nursery.The foundation seeds are produced by isolated propagation of the breeder’s seeds,and the sterile line seeds for field production are produced by isolated propagation of the foundation seeds.The breeder’s seeds of restorer line 8020 R are produced by bagging and selfing and selection in isolation zone(net) plant line nursery,and the foundation seeds are produced by re-propagation.The hybrid seed production requires better isolation conditions,which can be realized using natural forests,mountains,rivers,roads(railways,highways),houses,etc.,and the isolation distance in open areas is equal to or larger than 800 m.It also requires early sowing of the male parent,so that the male parent can make full use of the light and temperature resources before winter to grow vegetatively,forming strong male parent plants that can provide more pollen.The seeds of the male parent are sown on September 8-10,and those of the female seeds are sown on September 12-16.Quality test is conducted during breeding and seed production in accordance with relevant national standards.The purity standards of the parent seeds are as follows: purity≥99%,neatness≥98%,germination rate≥80%,moisture≤9%,glucosinolate content≤20 μmol/g,and erucic acid content≤0.5%;the quality standards for parental field production seeds are as follows: purity≥98%,neatness≥98%,germination rate≥80% and moisture≤9%;and quality standards for hybrid seeds: purity≥85%,neatness≥98%,germination rate≥80% and moisture≤9%.展开更多
This paper introduced the breeding process,characteristics,yield performance,disease resistance,quality characters,and suitable production areas of the new early-maturing oil sunflower variety"Xin Kui No.27"...This paper introduced the breeding process,characteristics,yield performance,disease resistance,quality characters,and suitable production areas of the new early-maturing oil sunflower variety"Xin Kui No.27",and summarized high-yield cultivation techniques based on large-area continuous cultivation demonstration to serve agricultural production.展开更多
To further specify significance and development orientation of Hainan National Breeding and Multiplication ( hereinafter referred to as HNBM) ,such concepts as value engineering,industrial cluster and regional economy...To further specify significance and development orientation of Hainan National Breeding and Multiplication ( hereinafter referred to as HNBM) ,such concepts as value engineering,industrial cluster and regional economy were introduced by analyzing its properties and interpreting relevant policies,its functions,value and development opportunities were analyzed from multiple perspectives. According to the comprehensive analysis,HNBM concerns national core political and economic benefits in strategic and fundamental sense,which is a result of powerful comprehensive functions of HNBM and need of modern agricultural development. In addition,HNBM has a bottleneck in the development of industrial cluster. Only co-promoted by the province and the ministry,and by applying top-level design,breaking the conditionality,integrating and optimizing existing resources,HNBM will be constructed into a high-new seed industry center.展开更多
基金the financial support,grants AGL2017-85603-P,PID2020-120281RB-100 and PID2020-117788RB-100 funded by MCIN/AEI/10,13039/501100011033grants SBPLY/21/180501/000111 and SBPLY/21/180501/000050 funded by JCCM by EU through Fondo Europeo de Desarrollo Regional+1 种基金supported by a UCLM scholarshipsupported by a JCCM scholarship
文摘Background Artificial insemination(AI)is a routine breeding technology in animal reproduction.Nevertheless,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI.In this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery vehicles.In this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during transport.Results With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in AndromedR.Samples containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 h.Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.Conclusions The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs.In view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.
基金Supported by Special Chinese Herbal Medicine Breeding Project of Provincial Department of Science and Technology(2016C02058)Key Provincial Research and Development Project(2017C02019)
文摘As a traditional precious Chinese herbal medicine,Anoectochilus roxburghii has the cooling and detoxifying effects,and can nourish yin to reduce pathogenic fire,and has anti-inflammatory and analgesic effects. In this paper,we summarized the research progress in detail about the biological characteristics,resource distribution,composition,medicinal value and rapid seedling breeding technology concerning the rare Chinese herbal medicine A. roxburghii,in order to provide a reference for the in-depth study and rational development and utilization of A.roxburghii.
基金Supported by China Agriculture Research System of MOF and MARA(CARS-14-1-12)Science and Technology Research Project in Key Fields of the Corps(2021AB011)Science and Technology Project of the Nine Division(2021JS007).
文摘The breeding technology of sunflower inbred lines with four generations in one year was explored through the research on the biological characteristics of sunflower itself and the breeding status of sunflower inbred lines using the young embryo culture technology,the greenhouse seedling technology and field test planting,based on sunflower field breeding and planting combined with greenhouse planting.The reasonable selection of planting sites as the core of the breeding technology of sunflower inbred lines with four generations in one year is a guarantee.Embryos can be taken 7-10 d after pollination to enter the next generation of cultivation,thus shortening the generational cultivation cycle.The establishment of a breeding method of sunflower inbred lines with four generations in one year reduces the cost of breeding and has a practical role and significance for the field of sunflower inbred line breeding technologies.
文摘Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,and results in a high yield of young abalones after being applied.
基金Supported by the Natural Science Foundation of Hubei Province
文摘The application of microbial technology to mineral processing hasbeen reviewed with respect to the bioleaching of metals fromminerals, the influence of biotreatment on flotation, the biobeneficication of nonmetallic minerals, and the biotreatment for minewaste reclamation. The application of microbial technology to mineralprocess- ing has shown the advantages of high efficiency, low en-Ergy and reagent consumption, low capital and operating Costs, andlow pollution of the environment.
文摘Food shortages arise more frequently owing to unpredictable crop yield losses caused by biotic and abiotic stresses. With advances in molecular biology and marker technology, a new era of molecular breeding has emerged that has greatly accelerated the pace of plant breeding. High-throughput genotyping technology and phenotyping platforms have enabled large-scale marker-trait association analysis, such as genome-wide association studies, to precisely dissect the genetic architecture of plant traits. Large-scale mapping of agronomically important quantitative trait loci, gene cloning and characterization, mining of elite alleles/haplotypes, exploitation of natural variations, and genomic selection have paved the way towards genomics-assisted breeding(GAB). With the availability of more and more informative genomic datasets, GAB would become a promising technique to expedite the breeding cycle for crop improvement.
基金Special Fund for Basal Scientific Research of Guangxi Academy of Agricultural Sciences(GNK 2021YT017,GNK 2017YM08)Science and Technology Development Fund of Maize Research Institute,Guangxi Academy of Agricultural Sciences(GYK 2017010)Natural Science Foundation Project of Guangxi(2018JJB130196).
文摘To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new big-ear maize variety Guidan 903 with high yield,stable yield and good quality was established by the Maize Research Institute of Guangxi Academy of Agricultural Sciences with two self-selected inbred line GRL17349 and GRL21191 as female and male parents seperately.The average yield of this variety were 7429.5 and 7000.5 kg/hm^(2)in Guangxi Maize Regional Trial and Production Test respectively,which increased by 6.3%and 5.6%than the control(Guidan162).It was approved by Guangxi Zhuang Autonomous Region Crop Variety Certification Committee in June 2020(Approval No.Gui sheng yu 2020083).Determined by the Cereal Quality Supervision and Testing Center of Ministry of Agriculture and Rural Affairs(Bejjing),the grain bulk density,ratio of crude protein,crude fat,crude starch and lysine of Guidan 903 were 782 g/L,9.41%,6.19%,71.45%,0.35%respectively.This variety has the characterisics of strong resistance to collapse and disease,good quality,high and stable yield and wide adaptability.Furthermore,its seed yield is high with easy production technology and low cost.
基金Supported by Science and Technology Innovation Projects of Jurong City(2012018)
文摘The artificial breeding technology for juvenile of Whitmania pigra was introduced in the paper,including selection of sites and water quality,construction of spawning pool,hatching pool and escape proof facilities,key technology of leech selection,feeding,cocoon hatching,juvenile feeding and management.
基金Supported by Key Sci-Tech Project of Hebei Provincial Science and Technology Department(16226320D)Modern Agricultural Industrial Technology System of the Ministry of Agriculture and Ministry of Finance(CARS-3-2-5)
文摘Cangmai 028 was selected and bred according to the climatic characteristic of the winter wheat in Heilonggang Basin taking 98(083)as the female parent and the Canghe 030 as the male parent by "two-row parallel alternate selection" method.Cangmai 028 has characteristics of high resistance,water-saving,high and stable yield,and rich genetic background.This study elaborated selection and breeding background and methods,characteristics,quality,and yield of Cangmai 028.
文摘The breeder’s seeds of 56 A are produced through individual selection,sib mating in pairs and selection in isolation zone(net) plant line nursery.The foundation seeds are produced by isolated propagation of the breeder’s seeds,and the sterile line seeds for field production are produced by isolated propagation of the foundation seeds.The breeder’s seeds of restorer line 8020 R are produced by bagging and selfing and selection in isolation zone(net) plant line nursery,and the foundation seeds are produced by re-propagation.The hybrid seed production requires better isolation conditions,which can be realized using natural forests,mountains,rivers,roads(railways,highways),houses,etc.,and the isolation distance in open areas is equal to or larger than 800 m.It also requires early sowing of the male parent,so that the male parent can make full use of the light and temperature resources before winter to grow vegetatively,forming strong male parent plants that can provide more pollen.The seeds of the male parent are sown on September 8-10,and those of the female seeds are sown on September 12-16.Quality test is conducted during breeding and seed production in accordance with relevant national standards.The purity standards of the parent seeds are as follows: purity≥99%,neatness≥98%,germination rate≥80%,moisture≤9%,glucosinolate content≤20 μmol/g,and erucic acid content≤0.5%;the quality standards for parental field production seeds are as follows: purity≥98%,neatness≥98%,germination rate≥80% and moisture≤9%;and quality standards for hybrid seeds: purity≥85%,neatness≥98%,germination rate≥80% and moisture≤9%.
基金Supported by China Agriculture Research System of MOF and MARA(CARS-14-1-12)Scientific and Technological Research Project in Key Areas of the Corps(2021AB011)Nine Division Science and Technology Planning Project(2021JS007)。
文摘This paper introduced the breeding process,characteristics,yield performance,disease resistance,quality characters,and suitable production areas of the new early-maturing oil sunflower variety"Xin Kui No.27",and summarized high-yield cultivation techniques based on large-area continuous cultivation demonstration to serve agricultural production.
基金Supported by Hainan Natural Science foundation(313114)Sanya Science Project(2012YD71)
文摘To further specify significance and development orientation of Hainan National Breeding and Multiplication ( hereinafter referred to as HNBM) ,such concepts as value engineering,industrial cluster and regional economy were introduced by analyzing its properties and interpreting relevant policies,its functions,value and development opportunities were analyzed from multiple perspectives. According to the comprehensive analysis,HNBM concerns national core political and economic benefits in strategic and fundamental sense,which is a result of powerful comprehensive functions of HNBM and need of modern agricultural development. In addition,HNBM has a bottleneck in the development of industrial cluster. Only co-promoted by the province and the ministry,and by applying top-level design,breaking the conditionality,integrating and optimizing existing resources,HNBM will be constructed into a high-new seed industry center.