Minimizing sperm oxidative stress using nanotechnology for breeding programs in rams

Background Artificial insemination(AI)is a routine breeding technology in animal reproduction.Nevertheless,the temperature-sensitive nature and short fertile lifespan of ram sperm samples hamper its use in AI.In this sense,nanotechnology is an interesting tool to improve sperm protection due to the development of nanomaterials for AI,which could be used as delivery vehicles.In this work,we explored the feasibility of vitamin E nanoemulsion(NE)for improving sperm quality during transport.Results With the aim of evaluating this proposal,ejaculates of 7 mature rams of Manchega breed were collected by artificial vagina and extended to 60×10^(6)spz/mL in AndromedR.Samples containing control and NE(12 mmol/L)with and without exogenous oxidative stress(100μmol/L Fe2+/ascorbate)were stored at 22 and 15℃and motility(CASA),viability(YO-PRO/PI),acrosomal integrity(PNA-FITC/PI),mitochondrial membrane potential(Mitotracker Deep Red 633),lipoperoxidation(C11 BODIPY 581/591),intracellular reactive oxygen species(ROS)production and DNA status(SCSAR)monitored during 96 h.Our results show that NE could be used to maintain ram spermatozoa during transport at 15 and 22℃for up to 96 h,with no appreciable loss of kinematic and physiological characteristics of freshly collected samples.Conclusions The storage of ram spermatozoa in liquid form for 2-5 d with vitamin E nanoemulsions may lead more flexibility to breeders in AI programs.In view of the potential and high versatility of these nanodevices,further studies are being carried out to assess the proposed sperm preservation medium on fertility after artificial insemination.

The Purification and Rejuvenation and Breeding Technology of Rice Variety Yunjing 29 with Fragrant and Soft Rice

Yunjing 29 bred by Institute of Food Crops of Yunnan Academy of Agricultural Sciences is a new conventional japonica rice variety with fragrant and sof rice, it was examined and approved by Yunnan Provincial Variety Examination and Approval Committee in March of 2011. The variety is early-maturing, high-yield and good-quality, and has resistances to lodging, blast and bacterial leaf blight, its rice is sweet soft, goluptious and lucidus as well as not coarse when it is cold, so it is a good commodity. To further promote the popularization and cultivation of the variety, maintain its characteristics of high quality and high yield, and prevent commingling and degeneration, the purification and rejuvenation as well as breeding technology of high-quality seeds were proposed after continuous exploration and study.

Breeding and Seed Production Techniques of New Aromatic Danxiangyouzhenliang Rice Variety with Good Quality and High Yield

Danxiangyouzhenliang rice is a high-quality rice variety derived from the hybrid breeding of Danxiang 12A and Xianghuixiangmiao R133.Its unique temperature-sensitive three-line characteristics endow it with significant advantages of high and stable yield,and its rice fragrance is exquisite and its taste is delicate.Danxiangyouzhenliang rice showed good resistance to rice blast and bacterial blight.On June 10,2021,Danxiangyouzhenliang rice passed the Guangxi Crop Variety Approval(Guishendao 2021074).Danxiangyouzhenliang rice is suitable to be planted as early and late rice in southern,central and northern Guangxi.This study summarized the breeding process and high-yielding seed production techniques of Danxiangyouzhenliang rice,in order to provide useful reference for rice breeders and growers.

On the Comprehensive Prevention and Control Technology of the Reproductive Organ Disease of Breeding Geese under the Anti-season Breeding Mode

Reproductive organ disease of geese is an endemic and multiple infectious disease in large-scale breeding of breeding geese, especially in anti-season production, which brings a great economic loss to goose production. To make effective prevention and control of reproductive organ disease of breeding geese under the anti-season breeding mode, the characteristics of control principles of infectious diseases of the poultry and the occurrence and prevalence of reproductive organ disease must be combined, so as to carry out scientific prevention and control.At the same time, according to the climate characteristics of summer, the feeding and management of breeding geese and water quality control should also be done well, and many corresponding measures should also be taken, thus obtaining better effect.

Research Progress on Rapid Breeding Technology of Anoectochilus roxburghii Seedlings

As a traditional precious Chinese herbal medicine,Anoectochilus roxburghii has the cooling and detoxifying effects,and can nourish yin to reduce pathogenic fire,and has anti-inflammatory and analgesic effects. In this paper,we summarized the research progress in detail about the biological characteristics,resource distribution,composition,medicinal value and rapid seedling breeding technology concerning the rare Chinese herbal medicine A. roxburghii,in order to provide a reference for the in-depth study and rational development and utilization of A.roxburghii.

Studies on the Technology for Mass Production of Abalone Breeding

Based on the traditional way of abalone breeding,the technological process of industrial abalone breeding suitable for the coastal areas of southern China has been developed.The process is simple and easy to handle,and results in a high yield of young abalones after being applied.

Breeding and High-yield Cultivation Technology of Wuqing No.1, a New Cultivar of Black Soybeans

The research introduced selection and breeding process, characteristics and high-yield cultivation technology of Wuqing No.l. Wuqing No.l, also named Gaoyou black soybean, is bred based on Danbo black soybean introduced from Japan in 1996 by Gaoyou Vegetable Cultivation Technology Station and Yangzhou Agricultural Technology Promotion Station. It was named in 2014 by Jinagsu Crop Cultivar Approvement Committee. The cultivar is high-yielding with high quality, big seeds, high resistance to stress, as well as high health-care value.

Breeding Technology of Sunflower Inbred Lines with Four Generations in One Year

The breeding technology of sunflower inbred lines with four generations in one year was explored through the research on the biological characteristics of sunflower itself and the breeding status of sunflower inbred lines using the young embryo culture technology,the greenhouse seedling technology and field test planting,based on sunflower field breeding and planting combined with greenhouse planting.The reasonable selection of planting sites as the core of the breeding technology of sunflower inbred lines with four generations in one year is a guarantee.Embryos can be taken 7-10 d after pollination to enter the next generation of cultivation,thus shortening the generational cultivation cycle.The establishment of a breeding method of sunflower inbred lines with four generations in one year reduces the cost of breeding and has a practical role and significance for the field of sunflower inbred line breeding technologies.

Analysis on the High-end Feeding System and the Application Scenarios on the Lake Sheep Barn Feeding Scale Breeding Technology Research

In this paper, we conduct research on the high-end feeding system and the application scenarios on the lake sheep barn feeding scale breeding technology research. Sheep farm reproduction for the main places, so make sure sheep pen set reasonable. At the same time, we should reduce/avoid adverse effects caused by the surroundings of the flock, and conducive to the sheep’s normal growth and reproduction.Goats engineering technology mainly includes process of design and construction, building facilities, environmental control, waste treatment technology, sheep product processing technology, such as sheep breeding engineering technology is connected to the biological technology. This paper integrates the related theories to propose the countermeasures that will be meaningful.

Doubled haploid technology and synthetic apomixis:Recent advances and applications in future crop breeding

Doubled haploid(DH)technology and synthetic apomixis approaches can considerably shorten breeding cycles and enhance breeding efficiency.Compared with traditional breeding methods,DH technology offers the advantage of rapidly generating inbred lines,while synthetic apomixis can effectively fix hybrid vigor.In this review,we focus on(i)recent advances in identifying and characterizing genes responsible for haploid induction(Hl),(ii)the molecular mechanisms of Hl,(ili)spontaneous haploid genome doubling,and(iv)crop synthetic apomixis.We also discuss the challenges and potential solutions for future crop breeding programs utilizing DH technology and synthetic apomixis.Finally,we provide our perspectives about how to integrate DH and synthetic apomixis for precision breeding and de novo domestication.

21世纪国外图书馆技术产业的历史考察——对Marshall Breeding图书馆技术产业报告的分析

文章梳理21世纪以来国外图书馆技术产业的发展脉络,总结发展特征和规律,对我国图书馆技术产业可持续发展具有参考价值。文章采集马歇尔·布汀2002-2022年发布的图书馆技术产业报告,以图书馆2.0和图书馆服务平台为标志性事件,将国外21世纪图书馆技术产业的发展划分为3个阶段,采用共词可视化技术依次概括出各阶段的发展重心,提炼出国外技术产业发展的总体特征。文章最后为我国图书馆技术产业的可持续发展提出若干启示。

Breeding of Brassica napus Cultivar Shuangyou 092 with High Oil Content and Its Fine-quality and High-yield Cultural Techniques

Shuangyou 092, a Brassica napus cultivar with high oil content, high quality and high yield, was bred by Institute of Industrial Crops of Henan Academy of Agricultural Sciences with Zhongshuang 9 as female parent, and the cytoplasmic male sterility maintainer line 32B as male parent. It was registered and released in 2014. The average yield was 2 858.5 kg/hm^2 in Henan province regional test of high quality, 12.43% higher than the check variety Fengyou 9 in 2010-2011 and 2011-2012. Its yield was 3 478.8 kg/ha in Henan Province productive test of high quality,9.52% higher than the check variety Fengyou 9 in 2012-2013. According to the test results in 2011-2012 and 2012-2013, the seeds quality of Shuangyou 092 was very good with near zero erucic acid （0.0%, 0.1%） in fat acid, 17.19 μmol/g and 19.78 μmol/g glucosinolates in its meal, the oil content of 46.60% and 45.76%. The cultivar was resistant （tolerant） to Sclerotinia, virus diseases, cold, and lodging. Its high yield and high quality can be obtained by planting in large scale, sowing at right time, rational close planting, balancing fertilizer and harvesting at right time.

Genomics-assisted breeding——A revolutionary strategy for crop improvement

Food shortages arise more frequently owing to unpredictable crop yield losses caused by biotic and abiotic stresses. With advances in molecular biology and marker technology, a new era of molecular breeding has emerged that has greatly accelerated the pace of plant breeding. High-throughput genotyping technology and phenotyping platforms have enabled large-scale marker-trait association analysis, such as genome-wide association studies, to precisely dissect the genetic architecture of plant traits. Large-scale mapping of agronomically important quantitative trait loci, gene cloning and characterization, mining of elite alleles/haplotypes, exploitation of natural variations, and genomic selection have paved the way towards genomics-assisted breeding（GAB）. With the availability of more and more informative genomic datasets, GAB would become a promising technique to expedite the breeding cycle for crop improvement.

Breeding and Application of Guidan 903,a New High-yield and Good-quality Maize Variety

To meet the demand of maize production in Guangxi and even the southwest region of China and realize the upgrading and undating of maize varieties which will continuously improve the yield and quality of maize,a new big-ear maize variety Guidan 903 with high yield,stable yield and good quality was established by the Maize Research Institute of Guangxi Academy of Agricultural Sciences with two self-selected inbred line GRL17349 and GRL21191 as female and male parents seperately.The average yield of this variety were 7429.5 and 7000.5 kg/hm^(2)in Guangxi Maize Regional Trial and Production Test respectively,which increased by 6.3%and 5.6%than the control(Guidan162).It was approved by Guangxi Zhuang Autonomous Region Crop Variety Certification Committee in June 2020(Approval No.Gui sheng yu 2020083).Determined by the Cereal Quality Supervision and Testing Center of Ministry of Agriculture and Rural Affairs(Bejjing),the grain bulk density,ratio of crude protein,crude fat,crude starch and lysine of Guidan 903 were 782 g/L,9.41%,6.19%,71.45%,0.35%respectively.This variety has the characterisics of strong resistance to collapse and disease,good quality,high and stable yield and wide adaptability.Furthermore,its seed yield is high with easy production technology and low cost.

Artificial Breeding Techniques of Whitmania pigra

The artificial breeding technology for juvenile of Whitmania pigra was introduced in the paper,including selection of sites and water quality,construction of spawning pool,hatching pool and escape proof facilities,key technology of leech selection,feeding,cocoon hatching,juvenile feeding and management.

Selection and Breeding of Drought Resistant,Water-saving and High-yield Wheat Variety Cangmai 028

Cangmai 028 was selected and bred according to the climatic characteristic of the winter wheat in Heilonggang Basin taking 98(083)as the female parent and the Canghe 030 as the male parent by "two-row parallel alternate selection" method.Cangmai 028 has characteristics of high resistance,water-saving,high and stable yield,and rich genetic background.This study elaborated selection and breeding background and methods,characteristics,quality,and yield of Cangmai 028.

Seed Propagation Technology and Quality Standards of Zhuoyou 1032

The breeder’s seeds of 56 A are produced through individual selection,sib mating in pairs and selection in isolation zone(net) plant line nursery.The foundation seeds are produced by isolated propagation of the breeder’s seeds,and the sterile line seeds for field production are produced by isolated propagation of the foundation seeds.The breeder’s seeds of restorer line 8020 R are produced by bagging and selfing and selection in isolation zone(net) plant line nursery,and the foundation seeds are produced by re-propagation.The hybrid seed production requires better isolation conditions,which can be realized using natural forests,mountains,rivers,roads(railways,highways),houses,etc.,and the isolation distance in open areas is equal to or larger than 800 m.It also requires early sowing of the male parent,so that the male parent can make full use of the light and temperature resources before winter to grow vegetatively,forming strong male parent plants that can provide more pollen.The seeds of the male parent are sown on September 8-10,and those of the female seeds are sown on September 12-16.Quality test is conducted during breeding and seed production in accordance with relevant national standards.The purity standards of the parent seeds are as follows: purity≥99%,neatness≥98%,germination rate≥80%,moisture≤9%,glucosinolate content≤20 μmol/g,and erucic acid content≤0.5%;the quality standards for parental field production seeds are as follows: purity≥98%,neatness≥98%,germination rate≥80% and moisture≤9%;and quality standards for hybrid seeds: purity≥85%,neatness≥98%,germination rate≥80% and moisture≤9%.

Breeding and High-yield Cultivation Techniques for Oil Sunflower New Variety"Xin Kui No.27"

This paper introduced the breeding process,characteristics,yield performance,disease resistance,quality characters,and suitable production areas of the new early-maturing oil sunflower variety"Xin Kui No.27",and summarized high-yield cultivation techniques based on large-area continuous cultivation demonstration to serve agricultural production.

Hybrid Rice Breeding in China

Hybrid rice technology in China was developed in three stages. From the 1950s onwards, hybrid rice researchers, as typified by Yuan Longping, sought out a feasible approach to utilizing rice heterosis, and developed the three-line indica hybrid rice. Led by the government, the hybrid rice technology R&D teams adopted the research mode of "large-scale cooperation"(大协作), and accomplished technical innovations in the three-line system, selective breeding, and production of hybrid seeds. In the ensuing decade, Yuan Longping and others discovered the photo-thermo sensitive genic male sterility(PTGMS) of rice and developed PTGMS lines, turning highly advantageous two-line rice breeding from scientific vision into reality. Over the next twenty-five years, China’s super-rice breeding program integrated the conventional, three-line, and two-line breeding methods with modern molecular biotechnology methods. For nearly half a century, China made outstanding attainments in acquiring quality strains that yielded enormous economic and social benefits. Throughout this process, factors such as urgent social demand for increased grain production and scientific research cooperation provided significant impetus.

Analysis and Research on the Functional Value and Development Opportunities of Hainan National Breeding and Multiplication

To further specify significance and development orientation of Hainan National Breeding and Multiplication ( hereinafter referred to as HNBM) ,such concepts as value engineering,industrial cluster and regional economy were introduced by analyzing its properties and interpreting relevant policies,its functions,value and development opportunities were analyzed from multiple perspectives. According to the comprehensive analysis,HNBM concerns national core political and economic benefits in strategic and fundamental sense,which is a result of powerful comprehensive functions of HNBM and need of modern agricultural development. In addition,HNBM has a bottleneck in the development of industrial cluster. Only co-promoted by the province and the ministry,and by applying top-level design,breaking the conditionality,integrating and optimizing existing resources,HNBM will be constructed into a high-new seed industry center.