The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Uni...The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost;cost of finished products and increases gross domestic products.展开更多
Background:This study was conducted to evaluate the feed nutritional value of brewers’spent grain(BSG)residue resulting from protease aided protein removal.The nutritional value was measured as nutrient content,gas p...Background:This study was conducted to evaluate the feed nutritional value of brewers’spent grain(BSG)residue resulting from protease aided protein removal.The nutritional value was measured as nutrient content,gas production,nutrient digestibility and fermentation characteristics in batch culture.Results:Protein extraction process decreased content of crude protein but concentrated the neutral detergent fibre(NDF)and ferulic acid in BSG residue.The changes in the chemical composition of BSG residue varied with enzyme and enzyme dosage.Digestibility of dry matter(DMD)and NDF of residue differed among proteases.Increasing alcalase dosage linearly decreased DMD,whereas,the DMD linearly increased as everlase or flavourzyme dosage increased.Compared with BSG,the DMD,gas production and fermentation acid concentration of BSG residues were lower,whereas NDF digestibility was higher.Conclusions:The substantially increased NDF content and improved in vitro NDF digestibility due to protease hydrolysis suggest that BSG residue can be potentially exploited as a viable fibre source for ruminant feeding.展开更多
This paper analyzes a pro-forma economic market and supply chain system for the reuse of a lignocellulose (brewer’s spent grain) in an industrial biotechnology environment. An extant literature review was conducted, ...This paper analyzes a pro-forma economic market and supply chain system for the reuse of a lignocellulose (brewer’s spent grain) in an industrial biotechnology environment. An extant literature review was conducted, followed by a technical analysis of BSG, and the development of a supply chain system and economic market analysis based upon a participant brewing company and industry experts. In this paper, it was found that, even with the potential for future improvements in the conversion of brewer’s spent grain (BSG) from an efficiency standpoint, this industrial residual is supply chain prohibitive as a biofeedstock in comparison to other lignocellulose materials, therefore, centralized market relationships would not be advantageous for sellers and buyers. Future research should consider the viability of centralized supply chain structures for alternatives that may exist as future bio-feedstocks.展开更多
文摘The properties of extracellular cellulase obtained from Bacillus species (FIIRO Strain B223) on supplying cellulose from Brewers Spent Grain (BSG) were studied. Generally, a crude cellulase activity between 11,757 Units to 13,930 Units was observed for both sources of carbon (BSG and crystalline cellulose). The optimum pH and temperature of 5.0, and 30°C - 40°C for both sources of carbon was observed. Heavy metals such as copper, zinc, and iron inhibited the activities of B223 cellulase while the cellulase activities on alkaline earth metal (manganese) were moderate. The co-factor behaviour of manganese ion was also established. Local production of cellulase in Nigeria using local raw materials such as Brewers Spent Grain reduces enzyme cost;cost of finished products and increases gross domestic products.
基金funded by AAFC Growing forward program(GF2#1542).
文摘Background:This study was conducted to evaluate the feed nutritional value of brewers’spent grain(BSG)residue resulting from protease aided protein removal.The nutritional value was measured as nutrient content,gas production,nutrient digestibility and fermentation characteristics in batch culture.Results:Protein extraction process decreased content of crude protein but concentrated the neutral detergent fibre(NDF)and ferulic acid in BSG residue.The changes in the chemical composition of BSG residue varied with enzyme and enzyme dosage.Digestibility of dry matter(DMD)and NDF of residue differed among proteases.Increasing alcalase dosage linearly decreased DMD,whereas,the DMD linearly increased as everlase or flavourzyme dosage increased.Compared with BSG,the DMD,gas production and fermentation acid concentration of BSG residues were lower,whereas NDF digestibility was higher.Conclusions:The substantially increased NDF content and improved in vitro NDF digestibility due to protease hydrolysis suggest that BSG residue can be potentially exploited as a viable fibre source for ruminant feeding.
文摘This paper analyzes a pro-forma economic market and supply chain system for the reuse of a lignocellulose (brewer’s spent grain) in an industrial biotechnology environment. An extant literature review was conducted, followed by a technical analysis of BSG, and the development of a supply chain system and economic market analysis based upon a participant brewing company and industry experts. In this paper, it was found that, even with the potential for future improvements in the conversion of brewer’s spent grain (BSG) from an efficiency standpoint, this industrial residual is supply chain prohibitive as a biofeedstock in comparison to other lignocellulose materials, therefore, centralized market relationships would not be advantageous for sellers and buyers. Future research should consider the viability of centralized supply chain structures for alternatives that may exist as future bio-feedstocks.
