The nucleotide sequence of small coat protein(CPS) gene of Broad bean stain virus(BBSV) was determined and compared with other comoviruses.The CPS gene of BBSV consisted of 687 nucleotides and encodes a putative prote...The nucleotide sequence of small coat protein(CPS) gene of Broad bean stain virus(BBSV) was determined and compared with other comoviruses.The CPS gene of BBSV consisted of 687 nucleotides and encodes a putative protein of 228 amino acid residues.The CPS sequence of BBSV and those of other comoviruses shared identities of 36.5%-58.9% and 35.2%-70.3% at the nucleotide and amino acid levels,respectively.The RT-PCR method specific for BBSV detection was developed based on the determined CPS sequence.The RT-PCR assay presented here allows,for the first time,rapid and specific detection of BBSV.展开更多
文摘The nucleotide sequence of small coat protein(CPS) gene of Broad bean stain virus(BBSV) was determined and compared with other comoviruses.The CPS gene of BBSV consisted of 687 nucleotides and encodes a putative protein of 228 amino acid residues.The CPS sequence of BBSV and those of other comoviruses shared identities of 36.5%-58.9% and 35.2%-70.3% at the nucleotide and amino acid levels,respectively.The RT-PCR method specific for BBSV detection was developed based on the determined CPS sequence.The RT-PCR assay presented here allows,for the first time,rapid and specific detection of BBSV.
