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Rifaximin discontinuation during broad-spectrum antibiotic treatment in critically ill patients with hepatic encephalopathy
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作者 Lorenzo Ridola Alessandro Mari 《World Journal of Hepatology》 2024年第11期1356-1360,共5页
Hepatic encephalopathy(HE)is one of the main complications of cirrhosis,characterized by a wide spectrum of neuropsychiatric alterations that lead to an increase in mortality,morbidity and recurrent hospitalizations.D... Hepatic encephalopathy(HE)is one of the main complications of cirrhosis,characterized by a wide spectrum of neuropsychiatric alterations that lead to an increase in mortality,morbidity and recurrent hospitalizations.Due to the central role in HE pathogenesis of ammonia and other neurotoxins primarily produced by the gut microbiota,the main therapeutic approaches for the treatment of HE are based on the modulation of the gut microbiota.Rifaximin is a non-absorbable broad-spectrum antibiotic,that is effective against ammonia-producing grampositive,gram-negative,and anaerobic species,approved for the treatment of HE in secondary prophylaxis.The chronic administration of rifaximin in this setting is associated with a lower risk of HE recurrence and mortality,while the role of rifaximin for the treatment of an overt-HE episode in inpatients is still unclear.Limited data exist about the coadministration of rifaximin and broad-spectrum antibiotics commonly used to treat concomitant infections,as patients receiving or recently treated with antibiotics were frequently excluded from clinical trials.In this editorial we comment on the article by Ward et al published in the recent issue of the World Journal of Hepatology.It is a single center,retrospective,quasiexperimental,pharmacist-driven protocol,with the aim to evaluate the feasibility and safety of rifaximin discontinuation in critically ill patients with HE and chronic liver disease receiving broad-spectrum antibiotic therapies in intensive care units.The study revealed no differences between the protocol and control group in terms of primary outcome(days alive and free of delirium and coma to day 14)and secondary outcomes which include:Intensive care mortality,intensive care length of stay,intravenous vasopressor requirement changes and adverse effects rate.Therefore,rifaximin discontinuation during broad-spectrum antibiotic therapy does not appear to negatively impact the clinical status of critically ill liver patients,with a similar safety profile and significant cost savings,as compared to the coadministration of rifaximin and broad-spectrum antibiotics.In agreement with Ward et al,a recently published double-blind,randomized controlled trial provided additional evidence to support the feasibility of withholding rifaximin during broad-spectrum antibiotic therapy in critically ill cirrhotic patients.However,given the limitations of these studies,further multicentric and prospective clinical trials,enrolling a larger sample of non-critically ill patients,are needed to better establish the role of rifaximin in this setting. 展开更多
关键词 Hepatic encephalopathy Rifaximin discontinuation broad-spectrum antibiotics Critically ill patients Intensive care unit
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Can rifaximin for hepatic encephalopathy be discontinued during broad-spectrum antibiotic treatment?
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作者 Chien-Hao Huang Piero Amodio 《World Journal of Hepatology》 2024年第2期115-119,共5页
Hepatic encephalopathy(HE)is a formidable complication in patients with decompensated cirrhosis,often necessitating the administration of rifaximin(RFX)for effective management.RFX,is a gut-restricted,poorly-absorbabl... Hepatic encephalopathy(HE)is a formidable complication in patients with decompensated cirrhosis,often necessitating the administration of rifaximin(RFX)for effective management.RFX,is a gut-restricted,poorly-absorbable oral rifamycin derived antibiotic that can be used in addition to lactulose for the secondary prophylaxis of HE.It has shown notable reductions in infection,hospital readmission,duration of hospital stay,and mortality.However,limited data exist about the concurrent use of RFX with broad-spectrum antibiotics,because the patients are typically excluded from studies assessing RFX efficacy in HE.A pharmacist-driven quasi-experimental pilot study was done to address this gap.They argue against the necessity of RFX in HE during broad-spectrum antibiotic treatment,particularly in critically ill patients in intensive care unit(ICU).The potential for safe RFX discontinuation without adverse effects is clearly illuminated and valuable insight into the optimization of therapeutic strategies is offered.The findings also indicate that RFX discontinuation during broadspectrum antibiotic therapy was not associated with higher rates of delirium or coma,and this result remained robust after adjustment in multivariate analysis.Furthermore,rates of other secondary clinical and safety outcomes,including ICU mortality and 48-hour changes in vasopressor requirements,were comparable.However,since the activity of RFX is mainly confined to the modulation of gut microbiota,its potential utility in patients undergoing extensive systemic antibiotic therapy is debatable,given the overlapping antibiotic activity.Further,this suggests that the action of RFX on HE is class-specific(related to its activity on gut microbiota),rather than drug-specific.A recent double-blind randomized controlled(ARiE)trial provided further evidence-based support for RFX withdrawal in critically ill cirrhotic ICU patients receiving broad-spectrum antibiotics.Both studies prompt further discussion about optimal therapeutic strategy for patients facing the dual challenge of HE and systemic infections.Despite these compelling results,both studies have limitations.A prospective,multi-center evaluation of a larger sample,with placebo control,and comprehensive neurologic evaluation of HE is warranted.It should include an exploration of longer-term outcome and the impact of this protocol in non-critically ill liver disease patients. 展开更多
关键词 Rifaximin discontinuation Hepatic encephalopathy broad-spectrum antibiotics Crit-ically ill Medical intensive care unit Pharmacist-driven protocol
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Improving treatment plan and mental health in children with abdominal infection for broad-spectrum bacterial infections
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作者 Gui-Bo Wang Xue-Feng Zhang +2 位作者 Bing Liang Jie Lei Jun Xue 《World Journal of Psychiatry》 SCIE 2024年第9期1319-1325,共7页
BACKGROUND Pediatric abdominal infection is a common but serious disease that requires timely and effective treatment.In surgical treatment,accurate diagnosis and rational application of antibiotics are the keys to im... BACKGROUND Pediatric abdominal infection is a common but serious disease that requires timely and effective treatment.In surgical treatment,accurate diagnosis and rational application of antibiotics are the keys to improving treatment effects.AIM To investigate the effect of broad-spectrum bacterial detection on postoperative antibiotic therapy.METHODS A total of 100 children with abdominal infection who received surgical treatment in our hospital from September 2020 to July 2021 were grouped.The observation group collected blood samples upon admission and sent them for broad-spectrum bacterial infection nucleic acid testing,and collected pus or exudate during the operation for bacterial culture and drug sensitivity testing;the control group only sent bacterial culture and drug sensitivity testing during the operation.RESULTS White blood cell count,C-reactive protein,procalcitonin,3 days after surgery,showed better postoperative index than the control group(P<0.05).The hospital stay in the observation group was significantly shorter than that in the control group.The hospitalization cost in the observation group was significantly lower than that in the control group,and the difference between the two groups was statistically significant(P<0.05).CONCLUSION Early detection of broad-spectrum bacterial infection nucleic acids in pediatric abdominal infections can help identify pathogens sooner and guide the appropriate use of antibiotics,improving treatment outcomes and reducing medical costs to some extent. 展开更多
关键词 Pediatric abdominal infection Nucleic acid detection of broad-spectrum bacterial infection Bacterial culture Drug sensitivity testing Treatment effect COST Mental health
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Assessment of the Effect of Environmental Factors on the Antagonism of <i>Bacillus amyloliquefaciens</i>and <i>Trichoderma harzianum</i>to <i>Colletotrichum acutatum</i> 被引量:2
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作者 Rababe Es-Soufi Brahim El Bouzdoudi +3 位作者 Mounia Bouras Mohammed L’Bachir El Kbiach Alain Badoc Ahmed Lamarti 《Advances in Microbiology》 2017年第11期729-742,共14页
The effect of temperature (18°C - 30°C), water activity (0.85 - 1) and pH (4 - 9) was studied by dual culture technique on the antagonism of Bacillus amyloliquefaciens and Trichoderma harzianum to Colletotri... The effect of temperature (18°C - 30°C), water activity (0.85 - 1) and pH (4 - 9) was studied by dual culture technique on the antagonism of Bacillus amyloliquefaciens and Trichoderma harzianum to Colletotrichum acutatum, responsible of strawberry (Fragaria x ananassa (Weston) Duchesne ex Rozier) anthracnose. The antagonistic bacteria’s strains behave significantly and differently according to the parameters studied. These results reveal useful information about the applicability of their biocontrol in agricultural culture with the change of environmental factors. 展开更多
关键词 antagonism ANTHRACNOSE Biocontrol Environmental FACTORS
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Studies on Rhizospheric Mycoflora of Tea (Camellia sinensis): In vitro Antagonism with Dominant Bacteria 被引量:1
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作者 SINGH Shuchi SOOD Anchal +3 位作者 SHARMA Shivesh KUMAR Vivek SINGH Kamal Deep PANDEY Piyush 《应用与环境生物学报》 CAS CSCD 北大核心 2007年第3期357-360,共4页
Species of Trichoderma, Penicillium, Aspergillus and Mucor were found to dominate the rhizosphere of tea bushes in different regions of the Indian Himalayas. The Himachal Himalayas were dominated by Monilia humiscola ... Species of Trichoderma, Penicillium, Aspergillus and Mucor were found to dominate the rhizosphere of tea bushes in different regions of the Indian Himalayas. The Himachal Himalayas were dominated by Monilia humiscola (45.08%) and Mucor hiemalis (33.11%). In the Uttaranchal Himalayas, Aspergillus clavatum dominated the rhizosphere (54.01%), followed by A. flavous (20.07%). Although the fungal isolates require a mesophillic temperature (15 ℃ to 35 ℃), the tea rhizospheric isolates tolerate a wide range of temperatures (5 ℃ to 45 ℃). When tested for pH tolerance, these fungi showed a wide range (2.0 to 13.0) of pH tolerance under in vitro conditions. Seasonal variations also influenced the colony forming units (cfu) of the rhizospheric fungi, with the maximum forming in November and March, and the minimum in December^February. During this period, the populations of the fungi were found inversely proportional to the bacterial populations. In addition, the dominant bacteria in the tea rhizosphere, i.e., Bacillus subtilis and B. mycoides, showed antagonistic activity against fungal isolates by inhibiting the growth and causing structural abnormalities in mycelium. Tab 5, Ref 展开更多
关键词 细菌 拮抗作用 真菌区系 丛矮病 优势菌种
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Identification and Antagonism Study of a Novel Chitinase-producing Bacterium Burkholderia Sp.C3 against Phytopathogenic Fungi 被引量:1
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作者 金虹 《成都医学院学报》 CAS 2006年第2期110-115,共6页
Through a modified agar well diffusion assay,antagonism of a novel chitinase-producing strain C3 against the phy- topathogenic fungi including Phoma wasabiae Yokogi,Cochlibolus Heterostrophus,Exserohilum Turcicum,Curu... Through a modified agar well diffusion assay,antagonism of a novel chitinase-producing strain C3 against the phy- topathogenic fungi including Phoma wasabiae Yokogi,Cochlibolus Heterostrophus,Exserohilum Turcicum,Curuvularia Lunata (Walk)Boed,Thantephorus cucumris,Fusarium graminearum was tested.The data showed that the crude extracts of strain C3 had stable antifungal activity in the range of pH 5.0 to pH 8.0.The active components were heat labile and sensitive to proteinase K.A series of experiments supported that the compound responsible for inhibitory aetivity appeared to be chitinase.The 16s rDNA analysis indicated that C3 was subject to genus Burkholderia.Phenotypic characterization of C3 was also consisted with the result of molecular identification. 展开更多
关键词 拮抗作用 植物致病病菌 鉴别 生物防治 几丁质酶 C3菌株 提取物 16s RDNA 伯克霍尔德菌
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Identification of A Strain HN-1 against Banana Wilt Disease and Determination of Its Antagonism
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作者 Shen Linbo Xiong Guoru +3 位作者 Dong Lisha Kong Ran Guo Ting Zhang Shuzhen 《Plant Diseases and Pests》 CAS 2013年第4期12-16,共5页
[Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. ... [Objective] The paper was to identify strain HN-1 against banana wilt disease and to determine its antagonism. [Method] The strain HN-1 was ob- tained from the soil in fields heavily infected by Fusarium oxysporum f. sp. cubense (FOC). Antagonism of the strain against F. oxysporum was tested via dual-cul- ture and inhibition test on spore germination. [Result] HN-1 effectively inhibited mycelial growth and spore germination of F. oxysporum, Strain HN-1 was identi- fied as BrevibaciUus brevis according to its characteristics in morphology, physiology and biochemistry and its 16S rDNA sequence. The strain showed high inhibition effect on 15 species of fungal pathogens in the dual-culture trials with fungal pathogens. [ Conclusion] The study provides theoretical basis for application of strain HN-1 in agricultural fields. 展开更多
关键词 Banana wilt disease BrevibaciUus brevis HN-1 antagonism
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Growth and Antagonism of <i>Trichoderma</i>spp. and Conifer Pathogen <i>Heterobasidion annosum</i>s.l. <i>in Vitro</i>at Different Temperatures
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作者 Vizma Nikolajeva Zaiga Petrina +5 位作者 Livija Vulfa Laura Alksne Daina Eze Lelde Grantina Talis Gaitnieks Anita Lielpetere 《Advances in Microbiology》 2012年第3期295-302,共8页
Variations in the radial growth rate of 24 isolates belonging to ten species of Trichoderma, three isolates of conifer pathogen Heterobasidion annosum s.s. and four isolates of H. parviporum were evaluated by incubati... Variations in the radial growth rate of 24 isolates belonging to ten species of Trichoderma, three isolates of conifer pathogen Heterobasidion annosum s.s. and four isolates of H. parviporum were evaluated by incubation on a solid malt extract medium at a temperature of 4℃, 15℃ and 21℃. Trichoderma antagonism against Heterobasidion was investigated in dual culture in vitro. The slowest rate of growth was referable to all seven strains of Heterobasidion spp. All Heterobasidion spp. strains were overgrown by 63% of Trichoderma spp. strains after two weeks at 21℃ and by 33% of strains at 15℃. 21% of Trichoderma strains did not grow and only four strains belonging to T. koningii, T. viride and T. viridescens demonstrated the ability to completely overgrow Heterobasidion spp. after two weeks incubation at 4℃. According to the antagonistic efficiency, Trichoderma strains were divided into five groups with an Euclidean distance of 25. The groups contained isolates from different species. It was suggested that selected psychrotrophic fast growing T. viride, T. koningii and T. viridescens strains could be examined in different substrate conditions as suitable antagonist agents for the control of H. annosum and H. parviporum. 展开更多
关键词 TRICHODERMA HETEROBASIDION antagonism GROWTH Rate Temperature
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Effect of Synergism and Antagonism between Metals on Toxicity in Soils
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作者 ZHOUDE-ZHI GUZONG-LIAN 《Pedosphere》 SCIE CAS CSCD 1991年第2期177-187,共11页
Synergism and antagonism of cadmium (Cd), copper (Cu) and selenium (Se) to biological toxicities in red soil, yellow brown soil and black soil were evaluated by MICROTOX method. The relation between forms of the teste... Synergism and antagonism of cadmium (Cd), copper (Cu) and selenium (Se) to biological toxicities in red soil, yellow brown soil and black soil were evaluated by MICROTOX method. The relation between forms of the tested metals in soil and the synergism or antagonism between them was also studied.Results showed that owing to the difference of soil chemical properties, toxicity of these metals in soils was different. In red soil with acid reaction and low in cation exchange capacity, antagonism occurred significantly between metals when they coexisted at high concentrations, while synergism occurred only under low concentrations. It is indicated that in red soil, toxicity of metals affected by synergism or antagonism depends on concentration of the metals present. For yellow brown soil and black soil with larger cation exchange capacity and lower exchangeable aluminium (A1), no toxicity of metals was observed even if metals were added to soil in high concentrations. Synergism and antagonism between Cd, Cu and Se were controlled by the forms of metals present. The amount of water-soluble metals was the most important factor in determining synergism and antagonism.In this paper, comparisons of synergism and antagonism between metals in soils and in water solutions were made. There occurred the synergism of metal toxicity in water solutions when the concentration of coexisting metals was high. This is just opposite to the case in soils. 展开更多
关键词 antagonism biotoxictty metal pollution SOIL synergism
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Antagonism of local isolates of Trichoderma spp. on citrus root rot disease by Fusarium solani in the mekong delta of vietnam
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作者 Duong Minh Jozef Coosemans +2 位作者 Le Lam Cuong Ester Vandersmissen2 Pham Van Kim 《浙江大学学报(农业与生命科学版)》 CAS CSCD 北大核心 2004年第4期392-392,共1页
The local isolates of Trichoderma spp. and Fusarium solani were colected from citrus orchards in the Mekong delta of Vietnam and isolated on PDA, PDB and TSM medium for antagonism and Koch’s postulate testing. The re... The local isolates of Trichoderma spp. and Fusarium solani were colected from citrus orchards in the Mekong delta of Vietnam and isolated on PDA, PDB and TSM medium for antagonism and Koch’s postulate testing. The results showed that the high chitinolytic enzymes content of Trichoderma isolates can antagonise with Fusarium solani isolates by preventing the germination of Fusarium macroconidia in in-vitro condition. There are five promising isolates of Trichoderma spp. having high antagonism with Fusarium solani. These Trichoderma isolates also grew well in rice straws, maize stems, weeds and water hyacinth biowaste materials. These results supply the promising trend for biological control of root rot disease on citrus orchards of the Mekong delta. 展开更多
关键词 镰刀霉 木霉素 拮抗作用 柑橘 几丁酶 根腐病 越南
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WORLD MILITARY SITUATION 2016: ANTAGONISM INTENSIFIES BUT CRISES REMAIN CONTROLLABLE
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作者 MENG XIANGQING MA FENG 《Contemporary World》 2017年第1期20-24,共5页
The world military situation in 2016was complex,military strategies of major countries undergoing profound readjustment,contest in emerging fields increasing and a new round of populism cropping up worldwide which res... The world military situation in 2016was complex,military strategies of major countries undergoing profound readjustment,contest in emerging fields increasing and a new round of populism cropping up worldwide which resulted in'Black swan events'happening one after another,major-country contest and geopolitical conflicts piling up and local 展开更多
关键词 US WORLD MILITARY SITUATION 2016 antagonism INTENSIFIES BUT CRISES REMAIN CONTROLLABLE
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Isolation,identification,and antagonism protein analysis of B34 against Thanatephorus cuc-umeris
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作者 ZHENG Aiping, Li Ping, SUN Huiqing, and WANG Lingxia, Rice Res Inst, Sichuan Agri Univ, Sichuan Agri Biotechnology Engineering Res Center, Wenjiang 611130, China 《Chinese Rice Research Newsletter》 2002年第3期21-21,共1页
A strain B34 against Thanatephorus cucumeris was screened from rice plants. Lab and field experiments showed that the control effects of this fungal strain were better than that of Jinggangmycin on PDA plate. Based on... A strain B34 against Thanatephorus cucumeris was screened from rice plants. Lab and field experiments showed that the control effects of this fungal strain were better than that of Jinggangmycin on PDA plate. Based on the chemical components of cell wall and physiological and biochemical characters of B34, the fungal was named as Pseudomonas aureofaciens. It was a new antagonistic strain against Thanatephorus cucumeris. 展开更多
关键词 Figure Isolation identification and antagonism protein analysis of B34 against Thanatephorus cuc-umeris
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从劳资对立到相互成就:对抗性劳动法制的反思和变革 被引量:1
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作者 史际春 赵忠龙 《经贸法律评论》 2024年第3期118-139,共22页
西方资本主义国家的劳动法制以“劳资对立”为基础和逻辑,背景是大机器生产时代阶级对立的雇佣关系和中重度体力劳动,我国现行劳动法制在一定程度上受其影响。社会主义不存在劳动者和企业之间关系的根本性矛盾,而且公有制在我国占主导地... 西方资本主义国家的劳动法制以“劳资对立”为基础和逻辑,背景是大机器生产时代阶级对立的雇佣关系和中重度体力劳动,我国现行劳动法制在一定程度上受其影响。社会主义不存在劳动者和企业之间关系的根本性矛盾,而且公有制在我国占主导地位,劳动者远超“劳工”范围;普遍的脑力劳动和轻微体力劳动,高度市场化和科技进步条件下的灵活生产、工作,冲击着僵化的劳动法制;随着社会进步,越来越多的人出于兴趣志向和事业追求而工作;中国共产党的领导,确保中国不存在也不允许出现既得“利益集团”。故应当在此新的历史条件和国情基础上,构建一种劳动者和企业相互成就的和谐劳动关系与包容、柔性的社会主义劳动法制,一体适用于各种经营性的雇佣及劳动合作,劳动事故和失业等风险概由社会保险暨社会承担以解决个别企业经营及市场竞争的后顾之忧。 展开更多
关键词 劳资对立 劳动者和企业相互成就 劳动法制 经营性雇佣 劳动合作
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妊娠期糖尿病患者血清LncRNA DANCR和miR-33a-5p水平表达对妊娠结局预测价值研究 被引量:1
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作者 王春燕 刘慧赏 马少未 《现代检验医学杂志》 CAS 2024年第3期84-89,共6页
目的 探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)患者血清长链非编码核糖核酸分化拮抗非蛋白编码RNA (long noncoding RNA differentiation antagonizing nonprotein coding RNA,LncRNA DANCR)、微小RNA-33a-5p (miR-33a-5p... 目的 探讨妊娠期糖尿病(gestational diabetes mellitus,GDM)患者血清长链非编码核糖核酸分化拮抗非蛋白编码RNA (long noncoding RNA differentiation antagonizing nonprotein coding RNA,LncRNA DANCR)、微小RNA-33a-5p (miR-33a-5p)水平表达对妊娠结局的预测价值。方法 选取2021年8月~2023年2月于衡水市第四人民医院产检和分娩的154例GDM患者为GDM组,并根据妊娠结局分为良好结局组(n=115)和不良结局组(n=39);同期收集24周葡萄糖耐量试验正常的149例健康孕产妇作为对照组。采用实时荧光定量PCR(qRT-PCR)检测血清LncRNA DANCR和miR-33a-5p水平;采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清LncRNA DANCR和miR-33a-5p预测GDM患者不良妊娠结局的价值;采用Pearson相关性分析GDM不良妊娠结局患者血清LncRNA DANCR,miR-33a-5p与空腹血糖(FBG)、空腹胰岛素(FINS)、稳态模式评估法计算胰岛素抵抗指数(HOMA-IR)的相关性;Logistic回归分析GDM患者不良妊娠结局的影响因素。结果 GDM组血清LncRNA DANCR水平(0.69±0.15)显著低于对照组(1.01±0.22),miR-33a-5p (1.59±0.40)及不良妊娠结局总发生率(25.34%)显著高于对照组(1.02±0.23,5.36%),差异具有统计学意义(t/χ^(2)=14.835,15.140,23.011,均P<0.05)。不良妊娠结局组血清LncRNADANCR水平(0.50±0.14)显著低于良好结局组(0.75±0.18),miR-33a-5p (2.00±0.58),FBG (8.97±0.66mmol/L),FINS (18.63±1.31pmol/ml)和HOMAIR (7.42±0.98)显著高于良好结局组(1.45±0.26,8.01±0.59mmol/L,14.32±1.29pmol/ml,5.10±0.86),差异具有统计学意义(t=7.895~17.961,均P<0.05)。LncRNA DANCR,miR-33a-5p单独及二者联合预测GDM患者不良妊娠结局的曲线下面积(area under curve,AUC)分别为0.820,0.819和0.897。GDM不良妊娠结局患者血清LncRNA DANCR与FBG,FINS,HOMA-IR呈负相关(r=-0.498,-0.513,-0.509,均P<0.05),miR-33a-5p与FBG,FINS,HOMA-IR呈正相关(r=0.517,0.494,0.507,均P<0.05)。LncRNA DANCR是影响GDM患者不良妊娠结局的保护因素(OR=0.804,95%CI:0.693~0.933,P=0.004),miR-33a-5p是影响GDM患者不良妊娠结局的危险因素(OR=2.747,95%CI:1.444~5.225,P=0.002)。结论 GDM不良妊娠结局患者LncRNADANCR降低,miR-33a-5p升高,二者均是不良妊娠结局的影响因素。 展开更多
关键词 妊娠期糖尿病 妊娠结局 长链非编码核糖核酸分化拮抗非蛋白编码RNA 微小RNA-33a-5p
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1株抗真菌贝莱斯芽孢杆菌(Bacillus velezensis)lut-Y1的鉴定及抑菌活性研究
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作者 袁惠君 余诗曼 +3 位作者 徐琰莹 袁毅君 冯欢 张欢欢 《中国畜牧兽医》 CAS CSCD 北大核心 2024年第6期2354-2364,共11页
【目的】探究1株来自枸杞果实的贝莱斯芽孢杆菌lut-Y1的生长特性及抑菌活性,为微生态制剂的开发和应用提供参考。【方法】通过形态观察和16S rDNA基因测序分析对该菌株进行鉴定;测定该菌株生长曲线及最适pH、碳源、氮源及盐耐受度;采用... 【目的】探究1株来自枸杞果实的贝莱斯芽孢杆菌lut-Y1的生长特性及抑菌活性,为微生态制剂的开发和应用提供参考。【方法】通过形态观察和16S rDNA基因测序分析对该菌株进行鉴定;测定该菌株生长曲线及最适pH、碳源、氮源及盐耐受度;采用平板对峙法观察该菌株对链格孢菌(Alternaria alternate)、小麦根腐离蠕孢(Bipolaris sorokiniana)和小孢壳二孢(Ascochyta leptospora)的颉颃作用,并分析该菌株发酵液对3种病原真菌的抑菌活性。【结果】lut-Y1菌株在LB、NA和PDA培养基上均生长良好。根据形态和16S rDNA基因序列特征将lut-Y1菌株鉴定为贝莱斯芽孢杆菌(Bacillus velezensis)。在LB液体培养基中,lut-Y1菌株在0~3 h为迟滞期,3~18 h为对数期,18~62 h为稳定期,62 h后进入衰亡期;最适pH为7.0,最适碳源和氮源分别为蔗糖和蛋白胨,对NaCl的耐受量不超过2.0 g/L。lut-Y1菌株对链格孢菌有明显的颉颃作用,对小麦根腐离蠕孢和小孢壳二孢的颉颃作用较弱。对峙平板的两菌交界处,链格孢菌基内菌丝细弱、弯曲;小麦根腐离蠕孢基内菌丝细胞壁被破坏,有泡状突起。发酵液添加量为25%时生长圈直径分别缩小72.80%和83.24%。【结论】贝莱斯芽孢杆菌lut-Y1具有较好的抑菌活性,可为开发以芽孢杆菌为主要活性成分的微生态制剂提供材料。 展开更多
关键词 贝莱斯芽孢杆菌 鉴定 生物学特性 颉颃作用 抑菌效果
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汉中地区天麻萌发菌遗传多样性和促萌效果分析
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作者 彭浩 曹丹 +3 位作者 司豆豆 张升明 张怀荣 李娜 《北方园艺》 CAS 北大核心 2024年第2期118-124,共7页
以汉中地区9株天麻萌发菌及天麻花粉种子为试材,采用拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等方法,研究了9株天麻萌发菌的遗传多样性、亲缘关系和促萌效果,以期为天麻萌发菌的种质资源管理与评价等提供参考依据。结果表明:9... 以汉中地区9株天麻萌发菌及天麻花粉种子为试材,采用拮抗试验、ITS序列测定、酯酶同工酶技术和种子拌播等方法,研究了9株天麻萌发菌的遗传多样性、亲缘关系和促萌效果,以期为天麻萌发菌的种质资源管理与评价等提供参考依据。结果表明:9株萌发菌根据两两拮抗试验的程度不同分为不显著、显著和极显著3类;根据ITS序列的差异分为Mycena sp.和Heydenia sp.2类;根据酯酶同工酶酶谱条带在80%的相似水平上可分为5类;9株萌发菌与天麻种子共生萌发,其中C3菌株菌丝生长速度最高为(0.30±0.01)cm·d^(-1),C2菌株的萌发率最高为64.66%±2.89%,C2和C3菌株的综合表现最佳。 展开更多
关键词 天麻 萌发菌 拮抗 ITS 酯酶同工酶
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登记对抗主义下相对性物权说之批判
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作者 李宗录 《山东科技大学学报(社会科学版)》 2024年第3期49-56,共8页
登记对抗主义物权变动规则由设立要件与对抗要件构成,其适用难点在于如何解释对抗要件所产生的登记对抗效力。相对性物权说在承认未登记物权仍为物权属性可对抗一般债权的前提下,主张未登记物权不能对抗特殊债权乃对抗要件发挥其登记对... 登记对抗主义物权变动规则由设立要件与对抗要件构成,其适用难点在于如何解释对抗要件所产生的登记对抗效力。相对性物权说在承认未登记物权仍为物权属性可对抗一般债权的前提下,主张未登记物权不能对抗特殊债权乃对抗要件发挥其登记对抗效力的必然结果,进而认为,在登记对抗主义下,依据合同设立的未登记物权具有相对性。然而,该学说偏离了物权界定规则与登记对抗规则的位阶关系,对域外法的借鉴未能作出契合本土的诠释,导致设立要件与对抗要件的功能错位。实质上,相对性物权说是以物权的相对性替换债权物权化。特殊债权优先受偿与登记对抗效力不存在必然联系,因而不应纳入登记对抗效力的调整范围。 展开更多
关键词 民法典 登记对抗主义 未登记物权 相对性 债权物权化
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正确认识保护妇女合法权益的核心要义及其价值追求
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作者 李明舜 《中华女子学院学报》 2024年第4期5-9,共5页
保护妇女合法权益和男女平等都是我国的重要法律原则;保护妇女合法权益是促进男女平等实现的必要手段,是为了更好地促进男女平等;保护妇女合法权益必须坚持尊重和保障人权的基本理念;保护妇女合法权益、促进男女平等是家庭和睦的重要基... 保护妇女合法权益和男女平等都是我国的重要法律原则;保护妇女合法权益是促进男女平等实现的必要手段,是为了更好地促进男女平等;保护妇女合法权益必须坚持尊重和保障人权的基本理念;保护妇女合法权益、促进男女平等是家庭和睦的重要基础,是社会文明进步的重要标志,是中国式现代化的重要内容。 展开更多
关键词 保护妇女合法权益 促进男女平等 反对性别对立
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嗜酸乳杆菌对3种弧菌的拮抗效果及对凡纳滨对虾肠道菌群结构的影响 被引量:2
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作者 刘钢 胡晓娟 +4 位作者 苏浩昌 徐武杰 徐煜 文国樑 曹煜成 《南方水产科学》 CAS CSCD 北大核心 2024年第2期83-91,共9页
使用益生菌是预防和控制水产养殖细菌感染的非抗生素方法之一。嗜酸乳杆菌(Lactobacillus acidophilus)可在机体内分泌抗菌物质,具有抑制有害菌生长、调节肠道微生态平衡和增强抗毒抗病能力等功能。研究了不同氧环境和不同培养基条件下... 使用益生菌是预防和控制水产养殖细菌感染的非抗生素方法之一。嗜酸乳杆菌(Lactobacillus acidophilus)可在机体内分泌抗菌物质,具有抑制有害菌生长、调节肠道微生态平衡和增强抗毒抗病能力等功能。研究了不同氧环境和不同培养基条件下嗜酸乳杆菌、副溶血弧菌(Vibrio parahaemolyticus)、哈维氏弧菌(V.harveyi)、溶珊瑚弧菌(V.coral-liilyticus)的生长情况,并通过牛津杯法和液体培养法结合PCR荧光定量技术、高通量测序技术,系统评价了嗜酸乳杆菌对上述弧菌的拮抗效果及对凡纳滨对虾(Litopenaeus vannamei)肠道菌群的组成和变化的影响。结果显示,在厌氧条件下,哈维氏弧菌的菌量显著高于在好氧环境培养后的菌量(P<0.05)。不同培养基配比对4种细菌的生长均具有显著性影响(P<0.05)。嗜酸乳杆菌对3种弧菌的生长均有良好的抑制效果。在对虾肠道菌群背景下,嗜酸乳杆菌对副溶血弧菌的生长有显著抑制作用(P<0.05)。处理组的弧菌属比例较对照组显著减少,高菌量组的有益菌属均显著增加。研究表明,嗜酸乳杆菌对上述3种弧菌的生长有良好的抑制效果,在对虾肠道菌群背景下对副溶血弧菌的生长也有良好的抑制效果,且能够优化菌群结构。 展开更多
关键词 嗜酸乳杆菌 弧菌 拮抗 凡纳滨对虾 肠道菌群结构
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烟草赤星病拮抗菌YZ01发酵条件优化及抑菌活性物质初探 被引量:1
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作者 张子奇 李光哲 +8 位作者 丁佳玉 王平平 谭嘉琦 常邦帅 艾绥龙 王娜娜 颜霞 杨明明 黄丽丽 《中国生物防治学报》 CSCD 北大核心 2024年第2期347-358,共12页
由链格孢菌Alternariaalternata引起的赤星病是烟草上的主要叶部病害之一,常常造成巨大的经济损失。对烟草赤星病菌具有显著拮抗活性的菌株YZ01进行鉴定、活性物质探究及发酵条件优化,可为烟草赤星病的防治提供菌种资源,为指导其实际生... 由链格孢菌Alternariaalternata引起的赤星病是烟草上的主要叶部病害之一,常常造成巨大的经济损失。对烟草赤星病菌具有显著拮抗活性的菌株YZ01进行鉴定、活性物质探究及发酵条件优化,可为烟草赤星病的防治提供菌种资源,为指导其实际生产应用奠定基础。通过形态学、生理生化特征及16S rRNA和gyrA基因序列系统发育分析,鉴定菌株YZ01为贝莱斯芽胞杆菌Bacillusvelezensis。无菌滤液抑菌试验发现贝莱斯芽胞杆菌YZ0110%无菌滤液对烟草赤星病菌Alternariaalternata的抑菌率为86.56%,对苹果腐烂病菌Valsa mali、辣椒疫霉病菌Phytophthora capsici、番茄灰霉病菌Botrytis cinerea、苹果炭疽叶枯病菌Colletotrichum fructicola的抑菌率为100.00%。进一步研究发现,贝莱斯芽胞杆菌YZ01发酵液可使烟草赤星病菌菌丝细胞结构受损,导致其原生质体外渗,从而抑制菌丝生长,其发酵液抑菌活性成分主要为脂肽及蛋白类物质。单因素试验法和正交实验优化菌株YZ01的最佳发酵培养基配方为葡萄糖0.50%、胰蛋白胨1.50%和磷酸氢二钾1.50%。发酵液稳定性测定发现,其抑菌活性受温度、pH和紫外处理的影响较小。综上所述,该结果为烟草赤星病的生物防治提供了菌种资源,也为后续田间应用奠定了基础。 展开更多
关键词 烟草赤星病 贝莱斯芽胞杆菌 拮抗作用 抑菌活性物质
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